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RISS 인기검색어
- 검색키워드
- 저자 : ( Ramadan Abdelsalam Shaban Bagar ) (검색결과 3 건)
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( Ramadan Abdelsalam Shaban Bagar ) 대한내과학회 2014 대한내과학회 추계학술발표논문집 Vol.2014 No.1
Background: Infections with hepatitis B virus (HBV) continue to be a worldwide problem and a considerable proportion of these infections usually progress to chronic infection and hepatocellular carcinoma. The available treatments of HBV infections include interferon- alpha (IFN-a) and nucleoside analogue agents (lamivudine, adefovir, entecavir, telbivudine, and others). it has become increasingly evident that lamivudine- resistant mutations occur naturally and exist in HBV carriers who have not received lamivudine therapy. The HBV isolates in Libya have not been characterized in terms of lamivudine drug resistance. Methods: One hundred and fi fteen clinical plasma specimens of HBV infected patients were collected and HBV-DNA quantifi cation were performed in all specimens by Rotor- Gene 3000™. INNO-LiPA HBV Drug assay were applied for HBV drug resistance in 57 isolates. All isolates were collected at Saint James medical Laboratory, Tripoli, where HBV-DNA quantity and HBV drug resistance were performed. Questionnaires completed for each patient including personal data (name, address, gender, age and nationality) and the “RoboGene HBV Quantifi cation Kits” allows both standardized purifi cation and real-time in vitro quantifi cation of HBV-DNA isolates via amplifi cation of a subsequence of the viral gene coding for the HBsAg. Results: This study was conducted during the period from August 20, 2009 to February 25, 2010. Serum samples were collected from 115 patients, 74 (64.3%) males and 41 (35.7%) females. Conclusions: HBV DNA polymerase has an activity of reversed transcription and a highly conservative YMDD order locating in the polymerase structural region C area, which is the combing and functioning site of LAM (nucleoside antiviral medicine). Lamivudine-resistant HBV strains with YMDD mutations are an important factor for the failure of chronic hepatitis B treatment. Although emergence of drug-resistant strains is closely related to the duration of the lamivudine treatment.
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( Ramadan Abdelsalam Shaban Bagar ) 대한내과학회 2014 대한내과학회 추계학술발표논문집 Vol.2014 No.1
Background: Hepatitis B virus (HBV) represents a global health challenge due to its worldwide distribution and serious complications. The HBV genotypes have different infi uence on clinical picture, response to treatment and the long-term prognosis. Eight genotypes have been identifi ed and a ninth is on the horizon, with distinct geographical distributions. Aim: The aim of this study was to investigate the prevalence of HBV genotypes in Libyan patients. Methods: Quantitative real-time PCR assay was carried out on 121 clinical specimens.The specimens were extracted and amplifi ed using INNO-LiPA HBV Genotyping Primers and run on a 2% agarose gel, then hybridized by INNO-LiPA HBV Genotyping assay that allows the identifi cation of HBV genotypes A to H. Results: DNA Quantity ranged from < 50 to 3.6x10 IU/ml, 85 specimens with DNA quantity more than 1.000 copies/ml were extracted and amplified, 60 specimens which had bands on 2% agarose gel were hybridized by the INNO-LiPA HBV genotyping assay. Three genotypes were detected; D in 54 specimens (90%), A and E in 1 specimen for each (1.70%) and 4 specimens (6.70%) were mixed genotypes D/E. Conclusions: This study indicates that the predominant genotype in Libyan patients is genotype D.
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( Shaban Ramadan Abdelsalam Bagar ) 대한내과학회 2014 대한내과학회 추계학술발표논문집 Vol.2014 No.1
Background: E. coli O157: H7 is a cause of acute infectious diarrhoea in humans and the leading cause of haemolytic uremic syndrome, especiallyamong children. E. coli O157:H7 was known as Verotoxin producing E. Coli (VTEC).It is also called as Shiga toxin producing E. coli(STEC). More than 50 serotypes of EHEC have been isolated. We undertook a study to Estimate the incidence of Escherichia coli O157:H7 infection in diarrheic children attending Ibn Sinna Hospital, Sirte - Libya Methods: 124 stool specimens of diarrheic children were collected at paediatric department in Ibn Sinna teaching hospital in the periodfrom September 2009 to may 2010. All the samples were cultured on SMAC.Non sorbitol - fermenting colonies are tested for the somatic O157 antigen before being confi rmed as E. Coli O157.The colonies are gram stained and confi rmed with O157 antiserum or latex reagent. These colonies were later biochemically confi rmed as E. coli using API20E and reported as presumptively positive for E. coli O157:H7. Results: 124 stool samples which were obtained from 124 diarrheic children contained 127 bacterial isolates belonging to 6 different genera or strains, as detected on SMAC plates Only 4 isolates were non-sorbitol fermenting colonies on SMAC agar. These sorbitol-negatives isolates were later identifi ed serologically and biochemically as: E. COLI O157:H7, one isolate; Shigella sonnei, one isolate; Enterobcater sp, two isolates; 123 isolates fermented sorbitol on SMAC agar, and later identifi ed serologically and biochemically as:Pseudomonas sp. Conclusions: Among 124 stool specimens, only 1(0.7%) was positive for E.coli O157:H7 Magnitude of the problem due to E. coli O157:H7 appears to be not much at Sirte but, because of its ability to cause complication, E. coli O157:H7 should be screened in routine culture and identifi ed. Out of 124 diarrhea samples tested, only four cases (3.2%).
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