기계적 자극을 이용한 인체 중간엽줄기세포로부터 유사-섬유모세포로의 분화 유도

최경민 ( Kyung Min Choi ),서영권 ( Young Kwon Seo ),윤희훈 ( Hee Hoon Yoon ),권순용 ( Soon Yong Kwon ),이화성 ( Hwa Sung Lee ),박용순 ( Yong Soon Park ),손영숙 ( Young Sook Son ),송계용 ( Kye Yong Song ),김영진 ( Young Jin Kim 한국조직공학과 재생의학회 2006 조직공학과 재생의학 Vol.3 No.4

Recently, in vitro reconstruction of anterior cruciate ligament(ACL) has been tried using tissue engineering technique, especially mesenchymal stem cell(MSC) is considered as a good cell source. In this study, we tried to find the effect of mechanical stimulus on the differentiation of bone marrow-derived MSC into ACL fibroblastlike cell using a cell training bioreactor imposing cyclic mechanical tension whose parameters were 120 min/day, 1 Hz, and 5% strain. After 2 weeks of culture, MSCs under cyclic tension showed more regularly oriented alignment and lower expression of CD73 and CD105 than control by microscopy and FACS analysis. In transcriptional level, type I collagen, type II collagen, fibronectin, elastin, α-smooth muscle actin mRNA expressions of MSCs under cyclic tension were higher than control and similar to native ACL. In conclusion, it is thought that mechanical cyclic tension induced the loss of stemness of MSC, therefore improved the differentiation into ACL fibroblast-like cell.

肝吸蟲症의 肝癌 誘發 可能性에 對한 發癌物質 3´-methyl-4-dimethylaminoazobenzene의 影響

閔弘基,鄭慶淑 梨花女子大學校 韓國生活科學硏究院 1986 韓國生活科學硏究院 論叢 Vol.37 No.-

原發性 肝癌 특히 膽管癌이 肝吸蟲症을 동반하고 있는 예가 간흡충증이 高率로 만연되고 있는 극동 및 동남아의 여러 지역에서 드물지 않게 발견되어 왔으며 이런 현상은 偶然의 合倂일 것이라고 주장되기도 하였으나 그 동안의 疫學的, 病理組織學的 및 實驗的 觀察로 제시된 결과는 이들 兩者 사이의 밀접한 相關性을 강력하게 시사하고 있다. 간흡충증으로 인하여 惹起되는 담관의 腺腫性 增殖과 담관상피세포의 粘液細胞化生은 動物宿主의 차이에 관계없이 나타나는 특징적인 병리조직학적 공통소견인 동시에 암으로 轉化될 수 있는 前殷階의 변화로 看做되고 있으나 간흡충감염만으로는 실험적으로 암을 유발시키지 못하며 某種의 發癌物質의 介入으로 이루어질 것이라는 의견이 支配的이다. 그러나 발암물질을 사용한 실험적 연구가 우리나라에서는 수행된 바 없었다. 본실험은 간흡충감염으로 유발되는 담관조직의 변화에 관한 根本 性狀을 해명하고 발암물징이 이에 미치는 영향을 규명하려는 노력의 一環으로 진행 되었다. 食品染料로 많이 쓰이고 있는 발암물질이며 axo화합물의 하나인 3‘-MeDAB을 간흡충감염 마우스에 투여함으로써 담관상피세포가 암세포로 轉化되어가는 移行期 所見을 경험할 수 있었으나 간흡충 또는 발암물질 단독투여의 경우에 있어서는 암발생 소견을 발견할 수 없었다. 이러한 결과를 근거로, 간흡충감염은 담관상피세포의 화생을 유발하는 先行的 要因의 役割을 하며 3‘-MeDAB은 화생된 세포를 자극하여 惡性化시키는 促進的 要因으로서 담관암 발생에 있어 相互 協同作用을 나타내는 것으로 고려되었다. The main objective of the present study is to observe the effect of a carcinogen, 3'-methyl-4-dimethylaminoazobenzene(3'-MeDAB), on bile duct epithelial cells in Clonorchis-infected mice. The animals used were 90 male BALB/c albino mice, and divided into 3 groups: group I, treated with 0.03% 3'-MeDAB(Kwasei Ind. Co., Seoul, Korea) alone for 12 weeks; group II, given approximately 200 Clonorchis metacercriae alone, and group III, given 200 metacercariae at the end of the 12 week treatment with 0.03% 3'-MeDAB. From the 16th week when the autopsies have commenced, 3 mice were removed at random from each group and examined grossly and histopathologically at 4 week interval up to the 52nd week from the beginning of the experiment. For histopathological examination, liver tissues were stained with hematoxylin-eosin. The results obtained are summarized as follows; 1. The gross findings and histopathological changes observed were remarkable in groups II and III, particularly in group III. Mild to severe mucinous cell metaplasia and goblet cell metaplasia were demonstrable in epithelial cells of the adenomatous hyperplastic glands and the biliary ductules peripheral to the second-order bile ducts. Whereas, in group I there were no significant tissue changes except the lower degrees of bile duct proliferation. cholangiofibrosis and inflammatory cell infiltration which were transient. 2. Mice in groups I and II did not develop tumors at any stage. On the other hand, there were strong histopathological evidences of neoplastic transformation of epithelial cells of the biliary ductules and hyperplastic glandular tissues in 2 out of 30 mice in Group III throughout the observation period. The cells were identified as those of transitional stages in the development of cholangiocarcinoma. With the overall findings it was clear that a carcinogen, 3'-MeDAB, played a potentiating role as a promotor for the turnover of metaplastic biliary epithelial cells induced due to Clonorchis infection into neoplastic cells in mice

중학생의 흡연 실태

천민경,박인숙 충남대학교 간호과학연구소 2002 충남대 간호학술지 Vol.5 No.1

The purpose of this study was to serve as a basis for improving the health promotion behavior of adolescents, by examining to what extent middle school students actually had a smoke, in consideration of home, social and school life variables. The subjects in this study were 840 students randomly selected from two middle schools in rural area and three in urban area, North Chung-cheng Province, which included 420 girls and 420 boys. The data were collected for 17 days from June 12 through 30, 2000. And the responses from 799 students were selected for final analysis. The questionnaire used in this study was consisted of 35 items regarding four areas: general characteristics, home variables, social variables and school life variables. The collected data were analyzed with SAS PC + program. The frequency and percentage were employed to find out the general characteristics of the subjects, and x2 test was conducted to determine the relationship of smoking behavior to home, social and school life variables. The findings of this study were as follows : 1. General Characteristics First, as a result of analyzing smoking behavior by grade, gender and place of residence, it' s found that the number of smoker student increased with the advance of grade. Boy students had a smoke, more than girl did. The percentage of smoker student was 75.66% of the boys and 24.34% of the girls. The smoking rate was higher in urban area than in rural one. Second, as a result of investigating when the smoker students started smoking by grade, gender and place of residence, the largest number of them began smoking in elementary school. Third, as a result of analyzing the motivation of smoking by grade, gender and place of residence, their smoking behavior was mostly prompted by curiosity. 2. Family Variable and Smoking Behavior First, the more their family was peaceful, the less they smoked. This factor made a significant difference in smoking behavior.(p<0.001). Second, regarding parental acceptance and parental rejection, the students whose parent had an acception attitude smoked less, and there was a significant difference between the groups(p<0.01). For parental autonomy and control, the students whose parent showed an autonomous attitude smoked less, and the difference between the two was significant(p<0.05). Third, whether or not their fathers smoked didn't give a significant impact on their smoking, but mothers' smoking yielded a significant difference(p<0.05). 3. Social Variable and Smoking Behavior First, those who had more smoker friends smoken more, and the nonsmoker students had no smoker friend or had just one or two. This difference was significant(p<0.001). Second, smoking was affected by time they spent to get together with friends after school, and there was a significant difference in this point between the smoker and nonsmoker groups(p<0.001). Third, 19.74% of the smoker students hung around after school with 6 friends or more, and the rate of the nonsmoker students who got together after school with 6 friends or more was 9.89%. The difference between the two was statistically significant(p<0.001). Fourth, the percentage of having the best friend, among those who had ever been punished in or outside school, was 26.97% of the smokers and 9.87% of the nonsmokers. The disparity between the groups was significant(p<0.001). 4. School Life Variable and Smoking Behavior First, the smokers mostly ranked low in their class, while the nonsmokers ranked high in many cases. This difference was significant(p<0.01). Second, 4.61% of the smokers stayed away from school without due notice three times or more for the last three months, and the same case for the nonsmokers was 0.77%. The difference between the two was significant(p<0.001).

소금 종류 및 농도에 따른 김치 효모균의 생육특성

한영숙,권민경,현영희,송주은,오지영 동아시아식생활학회 2001 동아시아식생활학회지 Vol.11 No.5

Effects of various types of salts (commercial low salt, sea salt, refined salt, and bamboo salt) and concentrations (0, 3.0 and 5.0%) on the growth of yeasts isolated from Kimchi were investigated. The isolated yeasts used in the study are as follows: Saccharomyces cerevisiae. Sporobolomyces albo-rubescens, Issatchenkia orientalis, Cryptococcus luteolous, Ustilago maydis. Candida humilis, Pichia onychis, Cadida nitratophila, and Pichia jadinii. The growths of the yeasts were inhibited against each salt concentration. The growths of Candida sp. isolated from the later stage of fermentation was strongly inhibited against 5% concentration of salt. On the other hand the induction phase of Issatchenkia orientalis isolated at the early stage of fermentation was the quickest among all the tested yeasts. Among the salts. bamoo salt was found to be strongest inhibitor of, the growth of yeasts.

상호학습형 학습활동지를 활용한 체육수업의 효과 및 교육적 의미 탐색

권민정,김경숙 이화여자대학교 동작과학연구소 2007 이화체육논집 Vol.10 No.-

This study aims to form the groundwork for realization of learner-centered physical education by selecting 'reciprocal style" of Mosston from among several physical education styles and applying it to the field of physical education in school to research educational meanings for development and application of worksheet of reciprocal style. In this study, with criterion-based selection, the researcher selected 5 participants from a class with 38 female students in the 10th grade of O high school which he/she is serving presently, conduct depth interview to them for collecting data and compiled files collecting 'worksheet of reciprocal style' which they recorded at school and personal reconsideration diaries as auxiliary data. Depth interview took semi-structured interview type and consisted of several contents of 'worksheet of reciprocal style' such as its effects, its advantages and its disadvantages, opinions to apply to another lessons, general comprehensive opinions, etc. Collected data was analyzed through the procedure of inductive content analysis, and files collecting 'worksheet of reciprocal style' and personal reconsideration diaries were analyzed by content analysis. To confirm reliability of the procedure of analysis and interpretation of data, member check, peer debriefing and triangulation were taken. As a result of applying worksheet of reciprocal style in practical physical education, he/she found out that it has educational meanings such as thought-seeking through learner-centered education, memory-recovery through record and saving, future-planing through reconsideration and plan, recovery of the groundwork of education for the whole man through integration of theory and practice.

고등학생의 운동행동변화단계에 따른 신체적 자기효증감과 자디존중감 분석

천경민,표내숙 釜山大學校 附設 體育科學硏究所 2006 體育科學硏究所 論文集 Vol.22 No.2

The purpose of this study was an analysis on physical self-efficacy and self-esteem of high school students in stages of change in exercise behavior. Subjects of this study were 514 high school students in Pusan. The means of measurement used were the stage of change in exercise behavior scale, physical self-efficacy scale and self-esteem scale. Data were analyzed using by X² analysis, One-way MANOVA, One-way ANOVA, Duncans multiple range as statical method. The level of significance of all hypotheses tested was 5% and the result were as follows. There was significant difference according to the stages of change in exercise behavior for high school students. And maintenance stage was higher than action and preparation stage, which were higher than precontemplation stage in the physical self-efficacy according to the stages of change in exercise behavior. There was no significant difference of self-esteem according to the stages of change in exercise behavior.

Astromicin sulfate의 약동학 및 안전성 평가

정숙인,김연숙,오원섭,복혜숙,김명민,최경업,김연화,김성민,백경란,송재훈 대한화학요법학회 2001 대한화학요법학회지 Vol.19 No.3

목적 : Astromicin은 aminoglycoside계 항생제와 유사한 화학구조와 특성을 지니고, 기존의 aminoglycoside와 유사하거나 향상된 임상효과 및 부작용을 지닌 것으로 알려져 있다. 저자들은 astromicin의 약동학적 특성을 분석하고, astromicin 투여 환자를 대상으로 한국인에서 그 임상적 및 세균학적 효과와 안전성을 평가하고자 하였다. 대상 및 방법 : 약동학적 지표는 정상성인 남자를 대상으로 astromicin 200㎎을 30분간 정주한 후 24시간까지 혈장 및 뇨 검체를 수집하여 one-compartment open model에 따라 분석하였다. 중증으 세균감염이 있는 18세 이상의 환자를 대상으로 병용 또는 단독 투여한 후 임상적 효과와 세균학적 효과를 판정하였고, 치료전과 후의 순음청력검사와 혈청 크레아티닌으로 이독성과 신독성을 평가하였다. 결과 : 정상 성인 남자 12명을 대상으로 약동학적 지표를 평가하였고, 최고혈장농도는 투여 종료시점(C_(0))에서 16.87±1.68㎍/mL였으며, 반감기는 1.86±0.43 시간 이었고, AUC_(0-12h)은 38.12±10.57㎍ㆍhr/mL, Vd는 0.18±0.02L/㎏, CL은 5.25±2.07L/hr였다. 이러한 약동학적 지표는 다른 aminoglycoside와 유사한 결과를 보였다. 59명의 환자를 대상으로 시행한 임상적 효과 분석에서는 단독요법을 시행한 50명 중 임상적 치유율 94%, 세균학적 치유율 100%를 보였고, 순음청력검사를 시행한 48례의 환자중 의미있는 청력감소 소견은 관찰되지 않았으며, 신독성 또한 전혀 나타나지 않았다. 결론 : Astromicin의 약동학적 지표는 다른 aminoglycoside의 약동학적 지표와 비슷한 양상을 보이므로 향후 astromicin의 약물농도 감시에 있어서 다른 aminoglycoside의 치료적 약물 농도 감시 방법과 유사한 방법을 적용할 수 있으리라 기대된다. 또한 임상적 치유율이 우수하면서 신독성이나 이독성이 적어 세균 감염 환자의 치료에 있어서 단독 또는 병합 요법으로 비교적 안전하게 사용할 수 있으리라 생각된다. Astromicin(Fortimicin®) has some characteristics in common with other aminioglycoside antibiotics, although it has a unique chemical structure, which is different from them. This study was performed to elucidate the pharmacokinetic (PK) parameters of astromicin following single-dose intravenous infusion of 200 mg and to evaluate clinical efficacy and safety of astromicin in Korean populations. PK parameters of astromicin were determined in 12 healthy volunteers (65.5±5.2㎏). The plasma and urine samples were collected up to 24hrs. PK variables were calculated by fitting individual concentration-time curves to a one-compartment open model. Plasma level at the end of infusion was 16.87?1.68 ㎍/mL and declined to 1.05±0.35 (㎍/mL 8hr later. The half-life was 1.86±0.43 hr. Apparent volume of distribution was 0.18±0.02 L/㎏g, and total body clearance was 5.25±2.07 L/hr. These values were similar to those of other aminioglycosides. Clinical efficacy and safety were eviuated in 59 patients with moderate to severe bacterial infections who needed parenteral antibiotics. Among 50 patients who recieved astromicin monotherapy, 49 (98%) had favorable clinical reponse and 28 (100%) had favorable bacteriological response. Serial audiograms revealed no change in all of 48 patients. No nephrotoxicity was observed in all patients. Conclusively, our data suggest that therapeutic drug monitoring of astromicin can be conducted in a similar fashion as other aminioglycosides and astromicin is a useful and safe antibiotic in the treatment of severe bacterial infections.

자유생활아메바의 isoenzyme 양상에 관한 연구

韓慶淑,閔得暎,蘇鎭琸 연세대학교 의과대학 1982 연세의대논문집 Vol.15 No.2

Begonia semperflorens 器內繁殖에 미치는 置床方法과 TIBA效果

金卿淑,閔炳訓,李承雨 경희대학교 부설 식량자원개발연구소 1993 硏究論文集 Vol.14 No.-

Begonia somperflorens 'Prelude Scarlet' 줄기組織의 再分化 및 生長에 미치는 NAA, TIBA 및 置床方法의 效果를 調査하기 위하여 遂行된 實驗結果를 要約하면 다음과 같다. 1. 不定芽 및 不定根의 再分化 및 伸長은 NAA 無處理區에서 가장 優秀하였다. 2. TIBA 處理에 의한 不定芽形成은 TIBA 0.01 및 0.05㎎/l에서 促進되었으며 TIBA 高濃度에서는 심하게 抑制되었다. 반면에 不定根의 形成은 TIBA 處理에 의해 抑制되었다. 3. 最長不定芽의 길이는 TIBA 無處理에서 가장 길었고 最長根은 TIBA 0.005㎎/l에서 가장 길었다. 4. 置床方法에 따른 再分化에 있어서 不定芽形成은 水平置床에서 가장 優秀하였고 不定根形成은 逆位置床이 水平置床과 垂直置床에 비해 높은 分化率을 보였는데 特히 TIBA 0.01과 0.05㎎/l에서 5培 以上의 많은 뿌리를 分化시켰다. 5. 置床方法別로 再分化된 最長길이는 水平置床은 不定芽의 生長, 垂直置床은 不定根의 生長에 가장 效果적이었고 逆位置床에서는 不定芽와 不定根의 길이가 가장 짧은 것으로 나타났다. Experiments were carried out to investigate the effects of NAA, TIBA and orientation methods on regeneration and growth in Begonia semperflorens "Prelude Scarlet" stem segments cultured in vitro. The regeneration and growth of adventitious shoot and root showed the best result without NAA in the medium. The concentrations of 0.01 and 0.05 ㎎/l TIBA promoted adventitious shoot formation, but higher concentrations considerably supressed. On the other hand, the formation of adventitious root was suppressed with all TIBA concentrations. The longest shoot length obtained without TIBA while length of the longest root did at 0.005㎎/l TIBA concentration. Adventitious shoot formation showed the best result in horizontal orientation. However, the formation of adventitious root showed higher regeneration rate in invented orientation compared with both horizontal or vertical orientation. Especially, the concentrations of 0.01 or 0.05㎎/l TIBA were regenerated adventitious root of 5 times or more in invented orientation. The growth of adventitious shoot and root was the most effective in horizontal and vertical orientation, respectively. The inverted orientation suppressed the growth of both adventitious shoot and root.

유니섹스 모드에 반영된 Dualism에 관한 연구

이민경,한명숙 服飾文化學會 1995 服飾文化硏究 Vol.3 No.2

The purpose of this study is to consider the internal meaning and the external form of Dualism in Unisex Mode ; to investigate the dualistic expression appeared on the Unisex Mode by analyzing the general fashion magazine. The results of the study were as follows : First, the phenomenon of Dualism in unisex Mode may be clues indicating that some roles, once assigned exclusively to either males or females, are becoming mutually shared or what may be called human roles. Second, the expression of Dualism in Unisex Mode whether direct away of symbolic way is represented by metaphoric manner such as unisex look(mono sex look, or trans sex look) with spirit of the time's Third, the formality and contents that related dualistic mode shows a various method in style, coordination of texture or item.