Transcriptional activation of hypoxia-inducible factor-1α by HDAC4 and HDAC5 involves differential recruitment of p300 and FIH-1

Seo, Hee-Won,Kim, Eun-Jin,Na, Hyelin,Lee, Mi-Ock Elsevier 2009 FEBS letters Vol.583 No.1

<P><B>Abstract</B></P><P>The interplay between hypoxia-inducible factor-1α (HIF-1α) and histone deacetylase (HDACs) have been well studied; however, the mechanism of cross-talk is unclear. Here, we investigated the roles of HDAC4 and HDAC5 in the regulation of HIF-1α function and its associated mechanisms. HDAC4 and HDAC5 enhanced transactivation by HIF-1α without stabilizing HIF-1α. HDAC4 and HDAC5 physically associated with HIF-1α through the inhibitory domain (ID) that is the binding site for factor inhibiting HIF-1 (FIH-1). In the presence of these HDACs, binding of HIF-1α to FIH-1 decreased, whereas binding to p300 increased. These results indicate that HDAC4 and HDAC5 increase the transactivation function of HIF-1α by promoting dissociation of HIF-1α from FIH-1 and association with p300.</P><P><B>Structured summary:</B></P><P>MINT-6802187:</P><P><I>HIF1 alpha</I> (uniprotkb:Q16665) <I>physically interacts</I> (MI:0218) with <I>FIH1</I> (uniprotkb:Q9NWT6) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-6802058:</P><P><I>HIF1 alpha</I> (uniprotkb:Q16665) <I>physically interacts</I> (MI:0218) with <I>HDAC4</I> (uniprotkb:P56524) by <I>pull down</I> (MI:0096)</P><P>MINT-6802021:</P><P><I>HIF1 alpha</I> (uniprotkb:Q61221) <I>physically interacts</I> (MI:0218) with <I>HDAC4</I> (uniprotkb:P56524) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-6802036:</P><P><I>HIF1 alpha</I> (uniprotkb:Q61221) <I>physically interacts</I> (MI:0218) with <I>HDAC5</I> (uniprotkb:Q9UQL6) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-6802102:</P><P><I>HIF1 alpha</I> (uniprotkb:Q16665) <I>physically interacts</I> (MI:0218) with <I>HDAC5</I> (uniprotkb:Q9UQL6) by <I>pull down</I> (MI:0096)</P><P>MINT-6802121, MINT-6802156:</P><P><I>P300</I> (uniprotkb:Q09472) <I>physically interacts</I> (MI:0218) with <I>HIF1 alpha</I> (uniprotkb:Q16665) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P>

Alkylhalide가 Mouse의 LDH isozyme 분포에 미치는 영향 : Cellulose Acetate Electrophoresis Method

권미경,김영옥,박은주,이동화,이명미,이문희,이양자 曉星女子大學校 藥學大學 學生會 1988 曉星藥誌 Vol.4 No.-

The effect of Carbontetrachloride administration on Lactatedehydrogenase isozyme patterns were analyzed by cellulose acetate electrophoresis for the brain, heart, kidney, lung, liver and mouse muscle. Each other tissue was found to have a characteristic distribution of these isozymes. At normal condition, aerobic tissue such as brain, heart, kidney, and lung were found to have all five isozymes from LDH_1 to LDH_5, while anaerobic tissues such as liver and muscle were found to have superiorly LDH_5 The toxicity of Carbontetrachloride administration was most prominence in the liver, and lung toxicity was occured also.

GIC를 활용한 도로중심의 건물주소 부여시스템

이미란,김부영,옥철영 울산대학교 1999 공학연구논문집 Vol.30 No.1

현행 국내 건물주소는 토지지번 중심으로 구축되어 교통 및 산업구조에서 물류비용을 증가시키는 요인이 되어 왔다. 내무부에서는 이러한 문제를 극복하기 위해 도로중심의 건물주소체계를 구축하고자 1997년부터 서울 강남구와 안양시를 대상으로 시범사업을 수행하였다. 시범사업에서는 먼저 도로의 길이를 건물수로 나눈 기초간격에 따라 기초번호를 구하고, 기초번호에 근거하여 수작업으로 건물주소를 부여하였다. 그러나, 이러한 방법에서는 기초간격이 도로길이와 건물수에 의해 산출됨으로써 도로사정에 따라 다르고, 수작업으로 부여한 도로중심의 주소체계를 검증하는 수단이 없었다. 본 논문에서는 시범사업에서 나타난 문제점을 해결하고 새로운 건물주소를 자동적으로 부여하기 위해 GIS를 활용한 도로중심의 건물주소 부여시스템을 제시했다. 이를 위해 시스템 설계의 기본원칙을 새롭게 설정하고, 공간정보와 속성정보가 구축된 GIS를 활용하여 시스템을 구현한다. 본 시스템에서 도로의 인접한 건물은 도로의 기점에서부터 건물까지의 도로경로거리와 방향에 따라서 건물주소가 부여되고, 골목길에 인접한 건물은 골목길의 시작점에 가상건물번호(X)를 부여하고, 골목길의 시작점에서부터 건물까지의 거리와 방향에 따라서 가상건물번호의 하위번호(n)를 부여하여 'Ⅹ-n'형태의 건물주소를 부여한다. 이와 같이, 도로에 인접한 건물과 골목길에 인접한 건물에 대해 서로 다른 방식으로 건물주소를 부여함으로써 차별화된 도로방식의 주소체계를 구축한다. 본 시스템은 일괄적으로 주소변환작업을 수행하며, 현재 사용하고 있는 토지지번 중심의 현주소와 도로중심의 신주소를 동시에 검색한다. Current lot-based building address system caused to increase the distribution cost of industry. Thus in order to decrease the cost, the Ministry of Home Affairs began to design and implement a pilot system for a road-based building address at KangNamGu and AnYang city in 1997. The pilot system calculated a basic number which was obtained from dividing the length of road by the number of building and assigned manually a road-based building address. But the basic length is different according to the length of road and the number of building and there is no verifying means of manually assigned address. This thesis presents a road-based building address assignment system using GIS that solves various problems in the pilot system and assigns a new building address automatically. This thesis establishes fundamental principles for the system design and implements the system using GIS in which spatial and attribute data are stored. In the system, the building address of road is assigned according to the direction and road path-distance from starting point of the road. The system assigns a virtual building address(X) to the cross point of adjacent road and alley, and assigns the building address(X-n) of alley to sub number(n) of the virtual building number according to the direction and distance from starting point of the alley. It assigns the building address of road and alley in different way so that it produces a distinctive road-based address organization. This system assigns a new building address in batch and searches current lot-based and new road-based address simultaneously.

중도·중복장애 학생의 성공적인 통합교육을 위한 교육적 지원에 대한 질적 연구 : 초등학교 통합학급 교사, 특수학급 교사 및 특수학교 초등교사의 인식을 중심으로

이미경,박경옥,한경근 국립특수교육원 2009 특수교육연구 Vol.16 No.2

본 연구는 초등학교에 근무하는 통합학급 교사, 특수학급 교사 및 특수학교 초등교사 15명을 대상으로 중도·중복장애 학생들의 성공적인 통합교육을 위해 교육적 효과가 좋을 것으로 기대되는 학생의 수준, 통합교육을 방해하는 요인, 그리고 교육적 지원에 관해 인식을 개별면담을 통해 알아보았다. 연구결과, 중도·중복장애의 통합 및 통합 가능한 학생의 수준에 대해서는 중도·중복장애의 통합교육의 효과성에 대한 논란만큼 교사들마다 인식의 차이를 보였으며, 중도·중복장애 학생의 통합교육에 대한 방해요인으로는 1) 장애학생의 문제행동, 2) 장애학생에 대한 인식 부족, 3) 체계적인 교육 및 행정적 전문성의 부족, 4) 심리적 어려움을 해결할 창구의 부족, 5) 장애학생에 대한 교육적, 의료적, 치료적, 심리적인 전문지식의 부족 등으로 나타났다. 중도·중복장애 학생을 위한 교육적 지원으로는 1) 중도·중복장애 학생에게 맞는 개별 처방적인 교육과정 편성의 운영의 내실화, 2) 개별화 교육의 강화 및 교사간 협력, 3) 통합학급 교사와 특수교사의 전문성 강화 등을 꼽고 있었다. 연구 결과를 바탕으로 중도·중복장애 학생들이 성공적인 통합교육으로 교육성 성취를 높일 수 있는 방안에 대한 논의를 하였다.

저열량 마요네즈의 소비욕구 조사

이미옥 영산대학교 2006 영산논총 Vol.16 No.-

In this research, I'm trying to investigate consumer's interest to nutritive food and healthy food and figure out consumers' thoughts about mayonnaise, one of the high fatted products, by means of answering sheet. At the result of this survey, respondents think they should ingest nutritive food daily (83.0% of the answerer) but they do not have assurance of nutritionally superior products and if there is an inexpensive healthy food which is turned out to be scientifically a good food. 85.0% of the answerers are aware of intaking oils and fats and 63.9% said they know that mayonnaise is a high fatted product. Inspite of these answering, 39.0% of the respondents eat squids with mayonnaise, which is the highest percentage of the responds. 78.0% of the answerers would purchase the low fatted and low cholesterol mayonnaise, if any, instead of ordinary one.

이문열과 위화 소설에 나타난 폭력성 비교연구

이미옥(Lee, Mi-Ock) 동북아시아문화학회 2018 동북아 문화연구 Vol.1 No.55

This study is going to figure out difference between Yu Hua and Lee Mun-yeol regarding their recognition of violence. It is a meaningful attempt to understand the ideological gap between the two authors and examine the detailed aspects of violence in the two countries which their ideological discourse is grounded on. In the 1980’s, Lee Mun-yeol began his journey of literature and created works actively, and it was the period that started with the Gwangju Democratic Movement against the military dictatorship. The 1980’s was the period when culture and ideology represented as ‘the National People’s Movement’ were prevalent; however, even in the circumstances, Lee Mun-yeol tried to dig deeply into the ultimate meaning of humans, not historic realism. As what is written on his novels, Lee Mun-yeol did not put his trust in the crowd realizing any true revolution even under the democratic system. He thinks that the people can never be the main agents of history due to their snobbish and selfish nature, and who grasps authority does not matter as authority will spoil, and the people will lead their lives according to that. He maintained that tendency consistently, and it is revealed in other works like 『Son of a Man』, too. On account of his tendency of writing works like that, Lee Mun-yeol was criticized by some critics as being “a conformer to the system”. On the contrary, Yu Hua did show changes gradually. In the 1990’s, he transferred from demagogic literature to folk literature. This is not unassociated with Yu Hua’s experience of Tiananmen Massacre in 1989. Although they failed to bring revolution, Yu Hua found individuals’ power and potential through the explosion of the people’s energy and cohesiveness then. Despite that enormous alteration, however, in Yu Hua’s novels, the people are still depicted as the victims of violence within the great tide of history and fragile individuals that can never escape from the bonds of destiny no matter how hard they try. They just appear to suffer pathetically and desperately in order to survive as a human that is more humane. Yu Hua focuses on the people’s joys and sorrows more like this while Lee Mun-yeol pays more attention to snobbery internalized in the crowd. But the aspects of violence cannot but

Elevated Levels of PDGF Receptor and MDM2 as Potential Biomarkers for Formaldehyde Intoxication

Lee, Min-Ho,Lee, Byung-Hoon,Shin, Ho-Sang,Lee, Mi-Ock Korean Society of ToxicologyKorea Environmental Mu 2008 Toxicological Research Vol. No.

Formaldehyde has been identified as the most prevalent cause of sick building syndrome (SBS), which has become a major social problem, especially in developing urban areas. However, studies on the molecular mechanisms associated with formaldehyde toxicity have been limited, probably because it is difficult to relate the experimental results obtained from in vitro studies to human exposure in vivo. Using polymerase chain reaction-based suppression subtractive hybridization, we recently identified 27 different formaldehyde-inducible genes including platelet-derived growth factor receptor alpha gene (PDGFRA) and mouse double minute 2 (MDM2) gene which were increased significantly in both formaldehyde-exposed human trachea cells, 680.Tr, and rat tracheas. To establish a possible relationship between induction of these formaldehyde-inducible genes and symptoms of SBS, we examined expression levels of these genes in peripheral lymphocytes of residents of new apartments. Here, we report that the expression of PDGFRA and MDM2 transcripts was significantly higher in peripheral blood lymphocytes obtained from 15 residents in new buildings than in seven control individuals. Our results suggest that the elevated levels of PDGFRA and MDM2 may be associated with the formaldehyde-induced pathophysiology that is closely related with SBS, and that they deserve evaluation as potential biomarkers for formaldehyde intoxication.

Regulation of Tumor Necrosis Factor-${\alpha}$-induced Airway Mucin Production and Gene Expression by Carbenoxolone, Prunetin, and Silibinin

Lee, Hyun-Jae,Lee, Su-Yel,Jeon, Byeong-Kyou,Lee, Jae-Woo,Lee, Mi-Nam,Kim, Ju-Ock,Lee, Choong-Jae The Korean Academy of Tuberculosis and Respiratory 2010 Tuberculosis and Respiratory Diseases Vol.69 No.5

Background: In this study, we tried to investigate whether carbenoxolone, prunetin, and silibinin affect tumor necrosis factor (TNF)-${\alpha}$-induced MUC5AC mucin production and gene expression from human airway epithelial cells. Methods: Confluent NCI-H292 cells were pretreated with each agent (carbenoxolone, prunetin, and silibinin) for 30 min and then stimulated with TNF-${\alpha}$ for 24 hours. The MUC5AC mucin gene expression and mucin protein production were measured by reverse transcription-polymerase chain reaction and enzyme linked immunosorbent assay, respectively. Results: Carbenoxolone, prunetin and silibinin inhibited the production of MUC5AC mucin protein induced by TNF-${\alpha}$; the 3 compounds also inhibited the expression of MUC5AC mucin gene induced by TNF-${\alpha}$. Conclusion: This result suggests that carbenoxolone, prunetin and silibinin can inhibit mucin gene expression and production of mucin protein induced by TNF-${\alpha}$, by directly acting on airway epithelial cells.

The association between dietary sodium intake and obesity in adults by sodium intake assessment methods: a review of systematic reviews and re-meta-analysis

Lee Jounghee,Sohn Cheongmin,Kim Oh-Yoen,Lee Young-Min,Yoon Mi Ock,Lee Myoungsook 한국영양학회 2023 Nutrition Research and Practice Vol.17 No.2

BACKGROUND/OBJECTIVES: The scientific evidence of a sodium-obesity association is limited by sodium intake assessments. Our specific aim is to synthesize the association between dietary sodium intake and obesity across the sodium intake assessments as evidenced by systematic reviews in adults. SUBJECTS/METHODS: A systematic search identified systematic reviews comparing the association of dietary sodium intakes with obesity-related outcomes such as body mass index (BMI), body weight, waist circumference, and risk of (abdominal) obesity. We searched PubMed on October 24, 2022. To assess the Risk of Bias in Systematic Reviews (ROBIS), we employed the ROBIS tool. RESULTS: This review included 3 systematic reviews, consisting of 39 unique observational studies (35 cross-sectional studies and 4 longitudinal studies) and 15 randomized controlled trials (RCTs). We found consistently positive associations between dietary sodium intake and obesity-related outcomes in cross-sectional studies. Studies that used 24-h urine collection indicated a greater BMI for those with higher sodium intake (mean difference = 2.27 kg/m2 ; 95% confidence interval [CI], 1.59–2.51; P < 0.001; I2 = 77%) compared to studies that used spot urine (mean difference = 1.34 kg/m2 ; 95% CI, 1.13–1.55; P < 0.001; I2 = 95%) and dietary methods (mean difference = 0.85 kg/m2 ; 95% CI, 0.1–1.51; P < 0.05; I2 = 95%). CONCLUSIONS: Quantitative synthesis of the systematic reviews has shown that crosssectional associations between dietary sodium intake and obesity outcomes were substantially different across the sodium intake assessments. We need more high-quality prospective cohort studies and RCTs using 24-h urine collection to examine the causal effects of sodium intake on obesity.

Development and Gene Expression of Porcine Cloned Embryos Derived from Bone Marrow Stem Cells with Overexpressing Oct4 and Sox2

Lee, Jeong-Hyeon,Lee, Won-Jae,Jeon, Ryoung-Hoon,Lee, Yeon-Mi,Jang, Si-Jung,Lee, Sung-Lim,Jeon, Byung-Geun,Ock, Sun-A,King, W. Allen,Rho, Gyu-Jin Mary Ann Liebert 2014 Cellular reprogramming Vol.16 No.6

<P>The present study compared the potential of porcine bone marrow mesenchymal stem cells (pBMSCs) at different passages as nuclear transfer (NT) donors and the developmental efficiency of NT embryos from donor cells transfected with/without Oct4 and Sox2. Early-passage pBMSCs showed higher proliferation and expression of Oct4 and Sox2 and differentiation potential into mesenchymal lineages than middle- and late-passage pBMSCs. Cleavage rate did not differ among pBMSCs at different passages, but NT embryos with early-passage pBMSCs and middle-passage pBMSCs transfected with Oct4 (Oct4-pBMSCs) had significantly (p<0.05) higher blastocyst development than those with middle-passage pBMSCs. The incidence of apoptotic bodies in NT blastocysts from late-passage pBMSCs and Sox2-transfected middle-passage pBMSCs (Sox2-pBMSCs) was significantly (p<0.05) higher than others. The transcriptional levels of Oct4, Sox2, Nanog, Cdx2, Dnmt3a, and Igf2r genes were significantly (p<0.05) higher in Oct4- and Sox2-pBMSCs NT embryos. Middle-passage pBMSCs NT embryos revealed lower transcriptional levels of Bcl2 than others, except Sox2-pBMSCs NT embryos. The transcriptional level of Bax increased gradually in NT embryos derived from pBMSCs following extended passages and was significantly (p<0.05) higher in Sox2-pBMSCs NT embryos. Our results demonstrated that early-passage pBMSCs are more potent in expressing transcription factors and displayed higher differentiation ability, and middle-passage pBMSCs transfected with Oct4 improved the developmental efficiency of NT embryos, suggesting that high Oct4 expression cells are more efficient as NT donors.</P>