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( Md Lemon Hasan ),( Mahmoud A. Elnaggar ),정윤기 한국공업화학회 2020 한국공업화학회 연구논문 초록집 Vol.2020 No.-
Extracted cell membranes derived lipid bilayers is currently promising strategy in biomedical systems due to its biocompatibility and versatility. In this study we fabricated a tethered lipid bilayer (TLB) system, with membranes extracted from endothelial cells, on titanium and embedded a synthesized selenium catalyst that mimics nitric oxide synthase (NOS) to achieve a continuous NO releasing system from metallic surfaces. Surface analysis techniques unveiled successful formation of the TLB with acceptable surface coverage. In vitro cell studies showed superior endothelial cell attachment and proliferation and inhibition of smooth muscle cell growth in comparison with bare titanium and artificial TLB that was prepared with synthesized phospholipids.
( Md Lemon Hasan ),김윤아,( So Young Chun ),( Seung Kwon You ),( Tae Gyun Kwon ),( Dong Keun Han ),( Won-gun Koh ),정윤기 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.1
Reneal tissue regeneration is expected to be a promising strategy, in context of currently limmited treatment and organ donation for cronic kideny disese. In recent year, extracellular matrix (ECM) derived scaffold, owing to its highly biocompatiblity and ability to guid renel tissue regeneration, have been widely investigated to treat kidney regeneration. In our previous study, magnesium hydroxide nanoparticles incorporated three dimensional porous PLGA scaffold was directly coated with fibroblast derived extracellular matrix. Inspired from previous study, here we have prepared nephron progenitor cell containing ECM surface- modified scaffolds for renal tissue regeneration. In in vitro cell studies with NPCs, modified scaffold showed improved cell viability and significantly increased growth factor release in respective time.
Md Lemon Hasan,Mahmoud A. Elnaggar,정윤기 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.1
Supported lipid bilayers membranes are growing interest as models of biological membranes for coating of implantable biosensing devices. Because of the limitation of bilayers which directly supported on glass are separated from the substrate by a thin film of water, bilayer formation on the tethered surface is the choice. Here, we prepared coated gold substrate with tethered lipid bilayer. Firstly, gold coated glass substrates were tethered by different types of thiol lipids. Secondly, 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) was used for the formation of supported lipid bilayers. The successful surface modification was verified by FT-IR, confocal microscopy and FRAP assay. Antifouling assay was conducted with quartz crystal microbalance with dissipation (QCM-D) apparatus and fluorescence microscopy. The result demonstrates the successful formation of tethered lipid bilayer on gold substrate and provides suitable coating system of implantable biosensing devices.
Neural Interfacing Biomaterials Surface-functionalized with Neural Specific Lipid Bilayers
Md Lemon Hasan,Ga Eul Kim,Mahmoud A. Elnaggar,정윤기 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.1
We hypothesized that probe supported with a cellular membrane possessing cell adhesion molecules (CAM) such as L1cam can promote neuronal adhesion and growth. For the fabrication of this bio-mimetic system, PC12 cells were differentiated into neural cells to induce the expression of the neural adhesive L1cam that can promote neurite outgrowth and impede astrocyte growth. The modified substrate has the enhanced air stability by tethering lipids on the surface of titanium and introducing the extracted membrane components by vesicle fusion method to form a tethered lipid bilayer. The successful surface modification was verified by FT-IR, confocal microscopy and FRAP assay. The modified titanium was resistant to protein adsorption. In vitro cell study confirmed that the natural tethered lipid bilayer (PM-TLB) provides highly biocompatible surface for neuron cells, in contrast limits the adhesion and proliferation of astrocyte cells.
Substrate-Tethered Artificial Membranes with Functional Membrane Proteins
Md Lemon Hasan,김가을,정윤기 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.2
Manipulating a cell/tissue interactive surface is a crucial way to develop long-term implantable materials. Here, we produced particular interfaces with cellular membrane proteins to modulate selective interactions with inherent cells. In the first study, for the fabrication of a neuro-specific interface, neural-specific cell adhesion molecules (CAM,L1) containing vesicles extracted from differentiated PC-12 cells was used to formed a lipid bilayer tethered on titanium surface. The modified surface was successful in air stability and biofouling standpoints. In-vitro study confirmed that modified substrate provides a highly biocompatible surface for neuron cells; in contrast, it limits the astrocyte cell activation and local inflammation. In the second study, we are investigating on lipid bilayers incorporating galectin-1 protein (Gal-1).
Hasan, Md Lemon,Kim, Boram,Padalhin, Andrew R.,Faruq, Omar,Sultana, Tamanna,Lee, Byong-Taek Elsevier S.A. 2019 Materials Science and Engineering C Vol. No.
<P><B>Abstract</B></P> <P>Bioglass-calcium phosphate cement (CPC) composite materials have recently received increased attention for bone regeneration purposes, owing to their improved properties in term of biocompatibility and bone ingrowths. In this study, an injectable bone substitute (IBS) system which utilizes bioglass microspheres incorporated into brushite based cement, was evaluated. The microspheres were synthesized with a simple and low sintering temperature process; there was no significant phase difference shown from the powder and good interactivity with cells was obtained. Furthermore, physical properties were optimized in microsphere incorporated brushite cement in order to investigate <I>in vitro</I> and <I>in vivo</I> performance. Accordingly, setting time and compressive strength were hardly altered until a microsphere content of 40% (v/v) was reached. The brushite (BR)/bioglass microsphere (BM) system showed excellent bioactivity to the <I>in-vitro</I> simulated body fluid test: dissolution ions from composite materials influenced apatite growth, countered acidic pH, and increased material degradation. In an <I>in-vitro</I> study with preosteoblasts (MC3T3-E1), BR/BM supported cell adhesion and proliferation, while cell differentiation experiments without osteogenic supplements, demonstrated that BR/BM induced osteogenic differentiation. A post-implantation study conducted in femoral defects showed higher materials degradation and bone formation in BR/BM than in BR. The faster dissolution of bioglass microspheres increased BR/BM composite resorption and hence facilitated bone tissue integration. Our findings suggest that bioglass microspheres incorporated in cement could potentially be used as an injectable bone substitute for bone regeneration applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> 45S5 bioglass microspheres were fabricated with sintering temperature of a 950 °C in order to incorporate into brushite cement. </LI> <LI> In the composite IBS system, the microspheres (40%) were well distributed and retained good handling properties for clinical application. </LI> <LI> Microspheres releasing ions actively guided cellular behaviour and supported for osteoblast differentiation </LI> <LI> The empty space produced by the degradation of microspheres from brushite prompted to neovascularisations and bone tissue integration. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Neuronal cell membrane immobilized on neural interface devices for competent tissue integration
( Md Lemon Hasan ),김가을,( Mahmoud A. Elnaggar ),( Goeen Jeong ),임충만,( Myoung-ryul Ok ),정윤기 한국공업화학회 2020 한국공업화학회 연구논문 초록집 Vol.2020 No.-
As a promising solution to this dilemma, we designed and fabricated titanium substrates covered with cell membranes extracted from differentiated PC12 cells to induce the surface adhesion of neural cell molecules, thus promoting neurite outgrowth and neural network formation on the surface. The modified substrates were prepared by pre-tethering of lipids on the surface of titanium and introducing extracted membrane components onto that as a membrane-like bilayer structure by vesicle fusion method. Successful surface modifications were confirmed via FT-IR, confocal laser scanning microscopy observation and fluorescence recovery after photo-bleaching (FRAP) assays. The coated interface showed significantly protect protein absorption. Furthermore, in vitro cell studies using microscopy and in situ fabricated mock neural sensors, revealed that the surface specifically encouraged adhesion and differentiation of neural cells when compared to astrocytes.
정윤기,김윤아,( Md Lemon Hasan ),( So Young Chun ),( Seung Kwon You ),( Tae Gyun Kwon ),한동근,( Won-gun Koh ) 한국공업화학회 2020 한국공업화학회 연구논문 초록집 Vol.2020 No.-
In recent year, renal tissue regeneration with the help of extracellular matrix (ECM) derived scaffold is expected to be a promising strategy. Extracellular matrix (ECM) derived scaffold owing to its highly biocompatiblity and ability to guid renel tissue regeneration. In our previous study, magnesium hydroxide nanoparticles incorporated three dimensional porous PLGA scaffold was directly coated with fibroblast derived extracellular matrix. Inspired from previous study, here we have prepared nephron progenitor cell containing ECM surface- modified scaffolds for renal tissue regeneration. In in vitro cell studies with NPCs, modified scaffold showed improved cell viability and significantly increased growth factor release in respective time. An 8-week post implantation study conducted on kidney model demonstrated that modified scaffold reduced the expression of fibrogenesis inducing cytokine marker and accelerated the renal tissue regeneration with a low inflammatory response.