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( Md Mahmudul Islam ),( Zahida Yesmin Roly ),( Young Sook Lee ),( Md Khalekuzzaman ) 한국육종학회 2014 Plant Breeding and Biotechnology Vol.2 No.1
Rice (Oryza sativa L.), as a cereal grain, is the most widely consumed staple food for a large part of the world`s human population especially in Asia. Three indica rice (Oryza sativa L.) cultivars namely BRRI dhan29, BRRI dhan56 and BRRI dhan57 were used in this study to investigate the regeneration ability in rice. Regeneration potential was found to be highest (80.00%) for BRRI dhan29 and lowest (70.00%) for BRRI dhan57. Consequently, the highly regenerating indica rice cultivar BRRI dhan29 was used for genetic transformation. Embryogenic calli induced after 20 days were used for genetic transformation in the experiment. Agrobacterium strain LBA4404 was transformed with pBI121 binary vector which contains kanamycin resistance gene as a selectable marker gene and GUS as a reporter gene. Calli infected with this strain were analyzed by using 5-bromo 4-chloro 3-indolyl- D glucuronide (X-gluc) as a substrate. Expression of gene was determined by using the transformed shoots and roots. Stable integration and expression of GUS gene were also confirmed by using PCR analysis. The frequency of transformation in terms of transient GUS assay was found to be 35.0 ± 2 S.E. This study will provide valuable information for genetic transformation in cereal crops.
Phytoremediation of volatile organic compounds by indoor plants : a review
김광진,Md. Khalekuzzaman,Jung Nam Suh,Hyeon Ju Kim,Charlotte Shagol,김호현,김형주 한국원예학회 2018 Horticulture, Environment, and Biotechnology Vol.59 No.2
Air quality in homes, offices, and other indoor spaces has become a major health, economic, and social concern. A plantbased removal system for volatile organic compounds (VOCs) appears to be a low-cost, environment-friendly solution for improving indoor air quality. This review presents and assesses VOC removal mechanisms that use plants and their associated microorganisms as well as the factors that influence the rate and efficiency of VOC removal. To increase removal efficiency, it is important to have a thorough understanding of the mechanisms of VOC degradation by plants and their associated microorganisms. The potential of plants and their associated microorganisms, whether present in pots or forced-air systems, to remove VOCs from indoor environments have been supported by a number of studies. Variations in removal efficiency depend on the plant species used, the chemical properties of the volatiles in question, and a cross-section of other internal and external factors. It is thus critical to select the right plants and use methods that reflect in vivo conditions. Indoor plants with superior air-purifying abilities have been extensively studied; however, the low rates of VOC removal efficiency in interior environments entail the need of more studies. For instance, factors that modulate VOC removal by plants, such as air circulation rate, light intensity, moisture status, and season need to be explored. Improving the efficiency of plants and their associated microorganisms for VOC remediation of indoor air is necessary to ensure sustainable and healthy indoor environments.