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Value of Blood Culture for the Ascetic Fluid in the Diagnosis of Spontaneous Bacterial Peritonitis
( Ali Kassem ),( Usama Arafa ),( Laela Yusef ),( Abdella Mo-hamed ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1
Aims: Liver cirrhosis is the end result of hepatocellular injury that leads to both fibrosis and nodular regeneration. Usual presentations are upper gastrointestinal bleed, ascites, spontaneous bacterial peritonitis (SBP), hepatic encephalopathy, hepatocellular carcinoma and hepatorenal syndrome. Inoculation of the ascetic fluid into blood culture bottles at the patient’s bedside resulted in more rapid detection of bacterial growth, and it is superior to inoculation of the ascetic fluid into conventional culture. We aim to compare the diagnostic accuracy of ascetic fluid inoculation into blood culture versus the conventional culture method in the diagnosis of SBP in patients attending Sohag university Hospital at Upper Egypt. Methods: One hundred consecutive cirrhotic patients (54 males; mean age: 62.02±years) were included, and a total of 100 paracenteses were performed. All ascetic fluid samples were analyzed using the cytology, neutrophils, lymphocyte count and culturing. The Culturing was done using both Conventional and Blood cultures technique. Results: In our study 47 patients of 100 cirrhotic patients had spontaneous bacterial peritonitis of them 32 of the inoculated sets demonstrated growth ; of which were 11(23,4%) set demonstrated growth by both conventional and blood culture bottles, and 21 set demonstrated growth by culturing in blood culture bottles alone. Conclusions: Ascetic fluid culture has an important role in the diagnosis and management of SBP, the ascetic fluid inoculation into blood culture bottles at the patient’s bedside resulted in more rapid detection of bacterial growth and it is superior to inoculation of the ascetic fluid into conventional culture.