정신분열증 환자의 말초임파구에서 Fluorodeoxyuridine에 의해 유도되는 Fragile Sites

박경란,김원식 충남대학교 의과대학 지역사회의학연구소 1996 충남의대잡지 Vol.23 No.2

The expression frequency of FUdR-induced fragile sites was examined in schizo phrenia patients to determine whether this parameter could be used as a marker of genetic susceptibility in at risk individuals. No difference was found in expression frequency between the schizophrenia patients and a group of normal individuals. This indicates that the expression frequency of fluorodeoxyuridine induced fragile sites is not a suitable marker for assessing genetic susecptibility in schizophrenia.

간질환환자의 말초임파구 염색체에서 Fragile Site의 의의

박경란,김수일,이영호,김원식 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.1

To understand chromosomal instability in hepatitis B virus related disease and significance of fragile sites in hepatoma, lymphocytes of peripheral blood from patients of hepatitis, liver cirrhosis and hepatoma were cultured. Fragile sites of chromosomes were counted by chromosome group and the results were as follows. 1. The frequencies of fragile sites were significantly increased in hepatitis, liver cirrhosis and hepatoma compared with control group. 2. The frequency of fragile sites by chromosome group were appeared in chromosome C, A, B and E group in turn on control group, chromosome C, A and B in turn on hepatitis and liver cirrhosis, and chromosome C, B and A in turn on hepatoma respectively. 3. The frequency of fragile sites was significantly increased in liver cirrhosis with HBsAg( + ) in comparison to HBsAg(-) liver cirrhosis. However no differences of frequency were founded in hepatoma by presence of HBsAg. 4. The common specific fragile sites of chromosome were appeared in chromosome 2q13, 5q34, 6p22 and 11q13, It is presumed that chromosomal instability by hepatitis B virus may induce hepatocellular carcinoma, and the common fragile sites found in chromosome 2q13, 5q34, 6p22 and 11q13 would be correlated with sites of oncogenes. However, more study needs to he done in correlation between fragile sites and oncogenes.

사람 말초혈액 림프구에서 FudR에 의해 유도되는 Fragile Sites

박경란,신웅기,박성국,김원식 충남대학교 의과대학 지역사회의학연구소 1996 충남의대잡지 Vol.23 No.1

Chromosomal abnormalities including fragile sites induced by 10 ㎍/ml and 20 ㎍/ml fluorodeoxyuridine (FudR) were studied in lymphocytes cultures of six healthy individuals. A significant decrease in mitotic indexes in respect to control cultures was observed with two FudR concentrations used. The cells showing chromosome aberrations and the total number of cytogenetic alterations were significantly increased both FudR treated cultures with respect to control cultures. Damaged cells showing chromosome aberrations and total number of chromosomal aberrations were not significantly increased in FudR 20 ㎍/ml treated groups compared to FudR lO㎍/ml treated groups. The most frequent type of chromosomal aberration is a gap. A site showing a gap or break was defind as fragile if it appeated in 1% of cells analyzed and in at least three of six individuals studied with the same culture tre atment. Using these criteria, fragile sites 3p14, 6p25, 3q32, 1Oq22, 12q21 and 13q21 were induced in different proportions by different concentration of FudR.

정신 분열증 환자의 말초혈액림프구에서 BrdU에 의해 유도되는 Fragile Sites

박경란 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.2

The expression frequency of BrdU-induced fragile sites was examined in schizophrenia patients to determine whether this parameter could be used as a marker of genetic susceptibility in at-risk individuals. No difference was found in expression frequency between the schizophrenia patients and a group of normal individuals. This indicates that the expression frequency of bromodeoxyuridine induced fragile sites is not a suitable marker for assessing genetic susceptibility in schizophrenia.

Methotrexate가 脾臟에 미치는 影響에 관한 硏究

朴景蘭,李永浩,金源式 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.1

In order to study the morphological changes of spleen after treatment with methotrexate, ICR mice were used as experimental animal. The experimental mice were divided into control, 50mg/kg and 500mg/kg of methotrexate treated groups. Methotrexate injected intravenously via tail vein once on the 8th day of pregancy. The mice were sacrificed on the 1st, 2nd 3rd and 4th day after treatment, the spleens were removed and fixed in 10% neutral buffered formalin solution for 48hrs. Spleens were cutted 5㎛ thick and stained with H-E, then observed under the light microscope. The results were as follows: 1. The size of spleen were reduced after MTX treatment more on dose day by day. 2. The periarterial lymphatic sheath (PALS) and germinal center were also reduced after MTX treatment. 3. The lymphocytes of white pulp showed nuclear pyknotic changes and many nuclear debris were found after MTX treatment. 4. The brown hemosiderin pigments were increased markedly in the red pulp of MTX treated groups. 5. Marked proliferation of fibrous trabeculae were found in the red pulp of MTX treated mice.

사람 말초혈액 림프구에서 Bromodeoxyuridine에 의해 유도되는 Fraglie Sites

박경란,김원식 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.2

Chromosomal abnormalities including fragile sites induced by 50㎍/ml and 100㎍/ml bromodeoxyuridine (BrdU) was studied in lymphocytes cultures of six healthy individuals. A significant decrease in mitotic indexes in respect to control cultures was observed with both BrdU concentrations used. The cells showing chromosome aberrations and the total number of cytogenic alterations were significantly increased both BrdU treated cultures with respect to control cultures. Damaged cells showing chromosome aberrations and total number of chromosomal aberrations were increased in BrdU, 100㎍/ml treated groups compared to BrdU 50㎍/ml treated groups. The most frequent type of chromosomal aberration is a gap. A site showing a gap or break was defind as fragile if it appeared in 1% of cells analyzed and in at least three of six individuals studied with the same culture treatment. Using these criteria, fragile sites 4q31, 5q31, 6p22, 6q13, 9q21, and 15q24 were induced in different proportions by different concentration of BrdU.

제 1형 신경섬유종증 환자에서 발생한 위장관 간질종양 1예

원경준,이준,변유미,조민근,한경택,김원,홍란,임성철,김경종,김영대,박찬국,김만우 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.2

Gastrointestinal stromal tumor (GIST) is the most common non-epithelial neoplasm arising in the gastrointestinal tract, but this tumor is rarely seen in association with type l neurofibromatosis (NFl) Generally, the majority of GISTs are isolated neoplasm and they are sporadically found in the stomach, but the GISTs in NFl patients are usually multiple and usually found in the small intestine. We report a case of multiple GISTs in the jejunum of a 63-year-old woman diagnosed as NFl accompanied by complication of gastrointestinal bleeding, In this case, Patient had multiple cafe-au lait spots and neurofibroma on skin and had freckling on axilla and groin, and then, we made a diagnosis of NFl. Gastrointestinal bleeding is controlled by resection of multiple GISTs. Generally, only 3-5% of all gastrointestinal bleeding comes from the small bowel. Causes of small intestinal bleeding are angiodysplasia, neoplasm, NSAIDs induced ulcer etc. but, If patients have NFl accompanied by complication of gastrointestinal bleeding, GISTs on small bowel must be considered.

교원의 교원능력개발평가 결과, 인구학적 특성과 전문성 인식의 관계

박지혜,이경란,문은주 이화여자대학교 교육과학연구소 2015 교육과학연구 Vol.46 No.4

This study aimed to provide useful information by investigating the relationships between the results of teacher evaluation for professional development, teachers’ demographic characteristics, and their perception of expertise. Among those who participated in the survey asking teachers’ perception of the 2014 teacher evaluation for professional development, the data from 1,622 teachers in the upper and lower limits of one standard deviation of teacher peer evaluation and student satisfaction were used. Study findings were as follows. First, statistically significant and positive correlations existed between teacher evaluation results and teachers’perception of expertise. Second, female teachers, those whose career spanned 5-15 years, elementary teachers perceived their expertise higher than those of male teachers, those whose careers did not span 5-15 years, and middle and high school teachers. Third, mean scores of expertise perception of teachers who had high scores on peer evaluation and student satisfaction were higher than those of other groups. Last, providing information to parents and appropriate evaluation were the key factors distinguishing those who had high scores on teacher evaluation and student satisfaction from those had high scores on teacher evaluation and low scores on student satisfaction. In addition, providing information to parents was the key factor differentiating those who had high scores on teacher evaluation and student satisfaction from those who had low scores on both teacher evaluation and student satisfaction. The significance of these study results was discussed along with limitations and suggestions for future research studies.

가정에서 제조된 재래식 된장, 간장, 고추장 중에 존재하는 Ochratoxin A의 효소면역분석법에 의한 잔존량 분석

김철재,박경란,송태희 숙명여자대학교 환경과학연구소 1995 환경과학 Vol.2 No.-

본 연구에서는 재래 방법에 따라 생산되고 있는 전통발효식품(된장, 간장, 고추장)에 함유되어 있는 Ochratoxin A(OT-A)를 면역학적 정량분석법인 Enzyme-Linked Immuno-sorbent Assay(ELISA) 법을 개발하여 분석하였다. 각 가정에서 생산하여 소비되는 장류 즉, 된장 13종, 간장 12종, 고추장 14종을 전국적으로 수거하여 분석을 실시하였다. OT-A를 정량 조사한 표준곡선의 작성 결과 ELISA 방법의 sensitivity는 20pg/assay 이었으며, 본 실험에서 사용한 면역분석법의 OT-A 회수율은 90%이상이었다. OT-A의 잔존량은 가정에서 생산하여 소비되는 된장이 7.1±3.7ng/g, 간장이 2.1±2.6ng/g, 그리고 고추장이 4.0±1.9ng/g이었으며, OT-A에 대한 오염도가 비교적 낮은 것으로 나타났다. 한편 OT-A의 가열안정성 시험에서는 60, 90, 120℃에서 120분까지 가열 처리하여 OT-A의 잔존량을 조사하였던 바 121℃의 고온에서 120분까지도 안정하였다. This study was undertaken to develop the analytical procedures, Enzyme-Linked Immunosorbent Assay(ELISA) for the quantitative detection of ochratoxin A(OT-A) in the home-made 13 Doenjang, 12 Kanjang, and 14 Gochujang collected throughout the country. The detection limit in ELISA was leveled as low as 20 pg/assay, and that the OT-A recorvery rates from sample clean-up were appeared to be more than 90%. Therefore, ELISA was the appropriate tool to detect the residual OT-A in the traditional fermented foods. The residual contents of OT-A in the home-made traditional fermented foods were 7.1 《3.7 ng/g for Deonjang, 2.1《2.6ng/g for Kanjang, and 4.0《1.9ng/g for Gochujang. Comparatively it was low,and OT-A seemed to be carried over Doenjang to Kanjang from Meju. At heating stability test of OT-A in the traditional fermented foods was it found to be stable even at 121℃ for 120 min.

5-Fluorouracil로 유발된 백서후지의 선천성 기형

김천호,박성국,김선,박경란,김원식 충남대학교 의과대학 지역사회의학연구소 1996 충남의대잡지 Vol.23 No.1

To study the teratogenic mechanism of 5-fluorouracil on the developing rat hindlimbs and to reveal the congenital anomalies induced by 5-FU, 25mg/kg of 5-fluorouracil was injected intramuscularly into S-D rats on gestational day 10.5. The rats were sacrificed under ether anesthesia on gestational day 11.5, 12.5, 13.5, 14.5, 15.5, 16.5 and 17.5. The whole uterine horns were removed for observing the congenital anomalies of the hindlimbs with stereoscope. Some fetuses were fixed by the neutral buffered formalin solution for LM exmination and some hindlimbs were fixed by 2.5% glutaraldehyde solution for TEM and SEM examination. After routine processing, LM and TEM examination were performed. The results were as follows ; 1. The mean survival rate of 5-fluorouracil-treated rat fetuses was decreased sign ificantly(p<0.01) compared with that of the control group. 2. The mean body weight of the 5-fluorouracil-treated rat fetuses on gestaticnal day 17.5 was significantly lower (p<0.01) than that of the control group. 3. The congenital anomalies on the hindlimbs of the rat fetuses were 4 digit, short digit, 3 digit, and 2 digit anomalies in that order. These anomalies occured moreon the right limb about 2.6 times than on the left limb. 4. On LM and TEM examination, 5-fluorouracil injured the apical ectodermal ridge epithelial cells and mesenchymal cells of hindlimb bud initially, but, this recovered around gestational day 14.5. According to the above results, 5-fluorouracil-induced congenital anomalies of the hindlimbs of rat fetuses may be caused by injury to the apical ectodermal ridge epithelial cells of limb bud. Apical ectodermal ridge epithelial cells that have recovered from injury around gestational day 14.5 may elicit changed information of abnormal patterning and differentiation into the underlying mesenchymal cells in progress zone, and the various digital anomalies, such as oligodactyly and short digit anomalies will be resulted.