Diagnostic Performance, Stability, and Usability of Self-Collected Combo Swabs and Saliva for Coronavirus Disease 2019 Diagnosis: A Case-Control Study

Choi Seong Jin,Jung Jongtak,Kim Eu Suk,Kim Hong Bin,Park Jeong Su,Park Kyoung Un,Lee Hyunju,Lee Eunyoung,Choe Pyoeng Gyun,Kim Ji-Yeon,Lee Eun Joo,Song Kyoung-Ho 대한감염학회 2022 Infection and Chemotherapy Vol.54 No.3

Background Self-sampling procedures to detect severe acute respiratory syndrome coronavirus 2 is important for patients who have difficulty visiting the hospital and may decrease the burden for health care workers (HCWs). The objective of this study was to evaluate the diagnostic performance, stability and usability of self-collected nasal and oral combo swabs and saliva specimens. Materials and Methods We conducted a case-control study with 50 patients with coronavirus disease 2019 (COVID-19) and 50 healthy volunteers from March, 2021 to June, 2021. We performed real-time reverse-transcription polymerase chain reaction to compare the diagnostic performance of self-collected specimens using positive percent agreements (PPAs). Results The PPAs between self-collected and HCW-collected specimens were 77.3 - 81.0% and 80.5 -86.7% for the combo swabs and saliva specimens, respectively. The PPAs increased to 88.9 - 89.2% and 81.2 - 82.1% with a cycle threshold value ≤30. Conclusion The diagnostic performance of self sampling was comparable to that of HCW sampling in patients with high viral loads and may thus assist in the early diagnosis of COVID-19. Background Self-sampling procedures to detect severe acute respiratory syndrome coronavirus 2 is important for patients who have difficulty visiting the hospital and may decrease the burden for health care workers (HCWs). The objective of this study was to evaluate the diagnostic performance, stability and usability of self-collected nasal and oral combo swabs and saliva specimens. Materials and Methods We conducted a case-control study with 50 patients with coronavirus disease 2019 (COVID-19) and 50 healthy volunteers from March, 2021 to June, 2021. We performed real-time reverse-transcription polymerase chain reaction to compare the diagnostic performance of self-collected specimens using positive percent agreements (PPAs). Results The PPAs between self-collected and HCW-collected specimens were 77.3 - 81.0% and 80.5 -86.7% for the combo swabs and saliva specimens, respectively. The PPAs increased to 88.9 - 89.2% and 81.2 - 82.1% with a cycle threshold value ≤30. Conclusion The diagnostic performance of self sampling was comparable to that of HCW sampling in patients with high viral loads and may thus assist in the early diagnosis of COVID-19.

Synthesis of Mannich Bases of Antineoplaston A10 and their Antitumor Activity

Choi, Bo Gil,Seo, Hee Kyoung,Chung, Byung Ho,Choi, Sang Un,Lee, Chong Ock 전남대학교 약품개발연구소 1995 약품개발연구지 Vol.3 No.1

Some Mannish bases of Antineoplaston A10 which is antitumor agent under clinical investigation were synthesized and tested for cytotoxicity. The tested compounds (2a, 2b, 2d) showed good activity comparable to that of carboplatin.

항암제 안티네오플라스톤 A10 의 동족체합성 및 항암 활성

최보길,서희경,김옥영,정병호,오인준,조원제,천승훈,박민수,최상운,이정옥 전남대학교 약품개발연구소 1997 약품개발연구지 Vol.6 No.1

Some analogs and their Mannish bases of Antineoplaston A10 (A10) were synthesized Chemical yield for the 2-(or 3-)thienyl, benzoyl, and phenylproptonyl analogs were hight but 1-naphthyl analog was synthesized in low yield. The Mannish bases formation of these analogs with morpholine went very well compared to other bases. 1-Naphthyl, 4-nitrobenzoyl. and phenylpropiortyl analogs of A10 showed weak in vitro activity but the other A10 analogs shaved weaker or no activity at 10-1000 ㎍/㎖. But their Mannish bases containing A10 analogs showed good in vitro activity compared to simple A10 analogs.

Synthesis of Mannich Bases of Antineoplaston A10 and their Antitumor Activity

Choi, Bo-Gil,Seo, Hee-Kyoung,Chung, Byung-Ho,Choi, Sang-Un,Lee, Chong-Ock The Pharmaceutical Society of Korea 1994 Archives of Pharmacal Research Vol.17 No.6

Some Mannich bases of Antineoplaston A10 which is antitumor agent under chlinical investigation were synthesized and tested fro cytotoxicity. The tested compounds (2a, 2b, 2d) showed good activity comparable to that of carboplatin.

Self-Assembled Hierarchical Superstructures from the Benzene-1,3,5-Tricarboxamide Supramolecules for the Fabrication of Remote-Controllable Actuating and Rewritable Films

Choi, Yu-Jin,Kim, Dae-Yoon,Park, Minwook,Yoon, Won-Jin,Lee, Yumin,Hwang, Joo-Kyoung,Chiang, Yeo-Wan,Kuo, Shiao-Wei,Hsu, Chih-Hao,Jeong, Kwang-Un American Chemical Society 2016 ACS APPLIED MATERIALS & INTERFACES Vol.8 No.14

<P>The well-defined hierarchical superstructures constructed by the self assembly of programmed supramolecules can be organized for the fabrication of remote-controllable actuating and rewritable films. To realize this concept, we newly designed and synthesized a benzene-1,3,5-tricarboxamide (BTA) derivative (abbreviated as BTA-3AZO) containing photoresponsive azobenzene (AZO) mesogens on the periphery of the BTA core. BTA-3AZO was first self-assembled to nanocolumns mainly driven by the intermolecular hydrogen-bonds between BTA cores, and these self-assembled nanocolumns were further self-organized laterally to form the low-ordered hexagonal columnar liquid crystal (LC) phase below the isotropization temperature. Upon cooling, a lamello-columnar crystal phase emerged at room temperature via a highly ordered lamello-columnar LC phase. The three-dimensional (3D) organogel networks consisted of fibrous and lamellar superstructures were fabricated in the BTA-3AZO cyclohexane-methanol solutions. By tuning the wavelength of light, the shape and color of the 3D networked thin films were remote-controlled by the conformational changes of azobenzene moieties in the BTA-3AZO. The demonstrations of remote-controllable 3D actuating and rewritable films with the self-assembled hierarchical BTA-3AZO thin films can be stepping stones for the advanced flexible optoelectronic devices.</P>

만성신경압박의 병리조직학적 고찰

조경석,이재수,백민우,하영수,강준기,송진언,최창락 대한신경외과학회 1990 Journal of Korean neurosurgical society Vol.19 No.5

Recently nerve entrapments or nerve root compressions are common clinical symdromes. However, a clear understanding of both pathophysiological and morphological changes is lacking and it may not be completely established in the experimental model for chronic nerve compression, compared with a surprising review of peripheral nerve exposed to various acute damage. Adult Sprague-Dawley rats weighing 250 to 300 g were used as the experimental model to elucidate histopathological changes in chronic nerve compression, which were produced by banding the sciatic nerve(normally 1.2㎜ in diameter) with the length of 1㎝ silastic tubings with inner diameter, 0.6㎜ (Group Ⅰ), 0.9㎜ (Group Ⅱ) and 1.5㎜ (Group Ⅲ) in each. Specimens were obtained for light and electron microscopic studies postoperatively at 1 and 3 months following by nerve conduction study. Grossly in group Ⅰ, the sciatic nerve was compressed to approximately 50% of its normal in cuff area and in thin strand on distal part at 1 month and more progressed at 3 months. The sciatic nerve of group Ⅱ demonstrated 75% of control and distal part in 50% compression at 1 month, but the nerve seemed not to be affected by tubing. Light microscopic findings revealed Wallerian degeneration and diminished large myelinated fiber particularly in the periphery of nerves with 34% of transverse neural percentage in group Ⅰ at postoperative 1 month. Above findings were progressed to epineurial scarring and fibrosis at 3 months. There were marked diminution and deformity in large myelinated fiber in group Ⅱ, but it was not more severe than the ones of group Ⅰ. Electron microscopic findings in this group revealed the appearance of small regenerating unit clusters and thinly myelinated fibers. In group Ⅲ, histological findings were not much different from that of normal nerve. Nerve conduction study revealed the decrease in conduction velocity to mean 10 M/sec in group Ⅰ at 1 month and no electrical conduction at 3 months. In group Ⅱ, diminution of conduction velocity in 73% of normal range at I1month was noted, and 82% at 3 months. These findings explain correspondingly the histopathological changes in part of chronic nerve entrapment syndromes and appeal the need of further investigation in this experimental model.

Quantification of Arbutin in Plant Extracts by Stable Isotope Dilution Gas Chromatography-Mass Spectrometry

Lee, Kyoung Bok,Choi, Jaehyuk,Ahn, Soon Kil,Na, Jong-Kuk,Shrestha, Krishna Kumar,Nguon, Samnang,Park, Sang Un,Choi, Sangho,Kim, Jae Kwang Springer-Verlag 2018 Chromatographia Vol.81 No.3

<P>Arbutin is a skin-whitening agent found in plants such as the Ericaceae species. Herein, we report the development and validation of a method based on gas chromatography-mass spectrometry for the analysis of arbutin in plant extracts. Prior to analysis, silylation with trimethylsilyl (TMS) reagent produced 5TMS and 4TMS derivatives, for which the peak area ratios varied significantly prior to sample injection. However, the use of [d (4)]-arbutin as an internal standard overcame this issue, with little variation in the observed peak area ratios. Method validation confirmed the excellent linearity (r (2) = 0.9989), recovery (accuracy 101.4-105.7%), precision (relative standard deviation ae<currency> 3.43%), limit of detection (0.03 mu g mL(-1)), and limit of quantitation (0.08 mu g mL(-1)) of this novel method. Finally, our method was applied to the detection of arbutin in various plant species. To the best of our knowledge, arbutin was detected and measured in Prismatomeris tetrandra and Phyllanthus reticulatus samples for the first time.</P>

Bioactive triterpenoids from twigs of <i>Betula schmidtii</i>

Park, Kyoung Jin,Subedi, Lalita,Kim, Sun Yeou,Choi, Sang Un,Lee, Kang Ro Elsevier 2018 Bioorganic chemistry Vol.77 No.-

<P><B>Abstract</B></P> <P>Investigation of the MeOH extract of <I>Betula schmidtii</I> twigs resulted in the isolation and identification of three new triterpenoids (<B>1–3</B>), along with ten known ones (<B>4–13</B>). The structures of new compounds (<B>1–3</B>) were elucidated by spectroscopic methods, including 1D, 2D NMR (<SUP>1</SUP>H and <SUP>13</SUP>C NMR, COSY, HSQC, HMBC, and NOESY), HR-MS, and chemical methods. All the isolated compounds were evaluated for their cytotoxicity against A549, SK-OV-3, SK-MEL-2, and HCT-15 cell lines. Compound <B>11</B> exhibited potent cytotoxic activities against four cell lines, and compounds <B>5</B> and <B>13</B> significantly induced nerve growth factor secretion in a C6 rat glioma cell line. Their anti-inflammatory effects were also assessed by measuring nitric oxide production in lipopolysaccharide–activated BV-2 cells. Compounds <B>7</B> and <B>12</B> displayed potent inhibition of nitric oxide production, without significant cell toxicity.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Three new triterpenoids, together with ten known ones were isolated. </LI> <LI> The chemical structures of the compounds were elucidated by NMR and MS. </LI> <LI> Compound <B>11</B> exhibited potent cytotoxic activity against four cancer cell lines. </LI> <LI> Compounds <B>5</B> and <B>13</B> showed significant neuroprotective effect without cell toxicity. </LI> <LI> Compounds <B>7</B> and <B>12</B> strongly inhibited nitric oxide (NO) production. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Survival and Migration of Intracerebrally Transplanted human Adipose Tissue-Stromal Cells after Transient Global Cerebral Ischemia in Rats

( Si Kyoung Jeong ),( Il Woo Lee ),( Seung Pill Choi ),( Un Jung Lee ),( Yeon Hee Chong ),( Heung Jae Chun ),( Kyu Nam Park ) 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.1

We investigated survival and migration of human adipose tissue-derived stromal cells(hATSCs) after transplantation into rat brains with induced transient global ischemia. Subcutaneous tissues were obtained from elective surgeries and hATSCs were cultured. We identified the characteristics of hATSCs as stem cells. Fifteen rats were randomly divided into the following three groups: group I, normal-hATSCs(n=5); group II, ischemia(n=5); and group III, ischemia-hATSCs(n=5). hATSCs were transplanted into the posterior periventricular region above the hippocampus in the normal group and in the ischemic group induced by the four vessel occlusion method. hATSCs had survived for 14 days after transplantation in both the normal and ischemic groups. We could find that hATSCs survived more longer in the ischemic rats(group III) until 39 days after transplantation. In two rats in the normal group, cells were not identified 27 days after transplantation. Grafted cells migrated in both ischemia-hATSCs and normal- hATSCs, but in the normal group, the distribution of most of the grafted cells was restricted to the site of transplantation. In ischemic rats, cells migrated more extensively than in normal rats. The medial migration of cells in the ischemic group had statistical significance(p<0.05). This study revealed that hATSCs survive and migrate after transplantation into rat brains with transient global ischemia.

혈관성 시상부 병변시 다발성 유발전위 검사의 의의

조경석,라형균,안명수,최창락,송진언 대한신경외과학회 1985 Journal of Korean neurosurgical society Vol.14 No.1

We reviewed the cases of 9 patients with vascular lesions in thalamus, 5 hemorrhage and 4 infarctions that were confirmed clinical and CT findings. Generally CT scan and clinical datas proved to be helpful to the diagnosis and prognosis to thalamic lesion. However, these findings are not exactly correlated to prognosis of patients. We can gain more informative factors to our patients for Multimodal evoked potential examination (MEP) to analysis. Especially in our cases, MEP findings proved to be the most effective results in the determinations of the prognosis. We review the literature and discuss the treatment and discuss the treatment and prognosis in thalamic lesion. Emphasis is placed on the current and optimal method of the evaluation of prognosis in thalamic lesions using MEP combined with the finding of CT scan and clinical status.