금식시 흰쥐 소장 점막 유당분해효소에 대한 갑상선 호르몬의 영향 및 이의 유전적 조절 기전

이국래,이동호,이한주,윤정환,김재규,정운태,정현채,송인성,김정룡 大韓消化器病學會 1996 大韓消化器病學會誌 Vol.28 No.6

DNA Microarray를 이용한 인유두종 바이러스(Human Papilloma Virus:HPV)의 진단

정운원,이승관,이창규,조경진,김성욱 高麗大學校 倂設 保健大學 保健科學硏究所 2001 保健科學論集 Vol.27 No.1

Human papillomavirus(HPV) has been known as one of the important pathogenic agent in uterine cervical carcinoma. The molecular works such as PCR enable the detection of large number of HPV genotypes obtained from viginal swab. Many of the PCR-based methods for HPV detection involve an amplification step followed by any of a number of methods for distinguishing different HPV types. In this study, we adopted the DNA chip technology enabling a HPV type-specific differentiation both low-risk group(type-6, 11, 34, 40, 42, 43, and 44) and high-risk group(type-16, 18, 31, 33, 35, 39, 45, 51,52,54, 56, and 58). MY09/MY11 and GP5+/GP6+primers covered LI region are used in nested PCR to improve PCR amplification. HPV type-specific probes for DNA chip were modified with NH2-C6, followed by spotting on silylated slides, washing slides and hybridization with each PCR products. Of 163 DNA samples chosen randomly, 42 samples were negative, 8 ones for low-risk group of HPV and 96 ones for high-risk group of HPV. Especially, co-infections with various HPVs were shown in 17 samples. A recent study found that multiple HPV is a factor in persistent HPV infection, resulting in the development of cervical dysplasia. This result emphasized the necessity to detect multiple HPV infection. The application of DNA-chip to determinate specific HPV typing will be a stronger candidate than any other PCR-based methods. Furthermore, the sequencing data of the positive PCR products were shown no discrepancy with DNA chip results. This means that DNA chip is very useful tool for both HPV detection and typing.

일측전정기과 손상 희쥐의 전정보상과정에서 뇌간 신경핵의 cAMP-Response Element Binding 단백질 활성화

윤상대,이정헌,김진호,김민선,박병림 대한이비인후과학회 2001 대한이비인후과학회지 두경부외과학 Vol.44 No.12

구획 화재시 창유리 파괴 현상에 관한 실험적 연구

이수경,김종훈,최종운,이정훈 韓國火災ㆍ消防學會 1998 한국화재소방학회논문지 Vol.12 No.3

본 연구는 실제 화재실험을 통하여 구획 화재 시 일어날 수 있는 창유리의 파괴 형태 및 시간 그리고 개구 조건과의 관계 등을 고찰하고자 했다. 화재 시 창유리의 파괴현상은 창호 유리 중간 부분과 프레임 안에 있는 유리부분의 현저한 온도차이로 인한 열응력으로 일어남을 확인했다. 실험 1-3은 프레임 내부와 유리면의 온도차가 233.4℃ 였으며, 실험 2-1은 138℃, 실험 2-2는 83.6℃의 차이를 보였다. 파괴 발생 시 실내의 온도는 실험 2-1의 경우 434.4℃, 실험 2-2는 83.6℃의 차이를 보였다. 파괴 발생 시 실내의 온도는 실험 2-1의 경우 434.4℃, 실험 2-2는 440.6℃ 실험 2-3은 400.9℃를 기록하여 화염이 직접 닿지 않는 경우의 균열은 400 ~ 500℃의 온도에서 발생한다. 화재로 인한 창유리(한글라스 플로팅 유리)는 1541.14kW의 화재에 노출되었을 시 열응력에 의한 파괴가 일어나기는 하나 유리면이 파괴에 의해 개구부가 되지 않는다. This is a study on the relation of window glass breaking time, shape, and vent condition in a compartment fire through the experiment. We recognize the phenomenon that window glass breakage in a compartment fire be arose from the thermal stresses due to the temperature temperature difference was 233.4℃ for test 1-3, 138℃ for text 2-1, 83.6℃ for test 2-2. The interior test 2-2, 400.9℃ for test 2-3. so if the flame didn't reach at the surface of window glass, the breakage of glass occur at 400℃ ~ 500℃. When the fire size reached to 1541.14kW, the window might be broken by thermal stress. But window glass was not collapsed.

박막 크로멜-알루멜 열전퇴형 교류-직류 변환기

정인식,김호운,김진섭,이정희,이종현,신장규,박세일,권성원 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

A planar chromel-alumel multijunction ac-dc converter was fabricated on a LPCVD Si_(3)N_(4)/SiO_(2)/Si_(3)N_(4)-diaphragm, prepared by silicon bulk micromachining, which thermally isolated a bifilar evanohm-heater and the hot junctions of a chromel-alumel thermopile from the silicon substrate. The voltage responsivity, the ac-dc transfer error, and the fluctuation of the output thermoelectric voltage of the converter were investigated. The respective voltage responsivities in air and in a vacuum of the converter were about 3.16 mV/mW and 9.41 mV/mW. The ac-dc voltage and the current transfer errors in air were about ±1.5 ppm and ±0.7 ppm in a frequency range from 10 Hz to 10 kHz, respectively. The fluctuation of the output thermoelectric voltage from 5 seconds to 120 seconds after preheating in air for 5 seconds was about 0.06 % for a heater input of dc 1 V.

WDM 시스템용 광대역 증폭기의 시뮬레이션

李鎬俊,沈雲用,鄭白鎬 湖西大學校工業技術硏究所 1999 工業技術硏究所論文集 Vol.18 No.-

In recent years, the biggest issue of WDM system is in increasing its bandwidth. Transmission bandwidth of WDM may be limited by many kinds of component, and the bandwidth plays impotent role in most of all optical amplifiers. Transmission bandwidth of recent WDM is limited in about 40 nm. This limit depends on EDFA(?? Fiber Amplifier) gain bandwidth. To overcome this problem, we may use L and S band using Yb/Nd doped fiber amplifier. Wideband amplifier, which is consisted of RFA for L band and EDFA for M band using single pump laser diode, is presented. The amplifiers have been analyzed numerically for the design.

졸 - 겔법에 의한 다공성 알루미나 미분체 제조에 관한 연구 : 2 . 물과 알콕시드의 비가 알루미나 미분체의 결정화 공정에 미치는 영향 2 . The Effect of [ H2O ] / [ Al - alkoxide ]on The Crystallization Mechanism of As - Prepared Particles

이성원,박형상,윤호성,황운연,구기갑,유승준,김용렬,이정운 한국화학공학회 2001 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.39 No.2

알루미늄 알콕시드의 반응속도를 제어하기 위한 혼합용매와 입자제조 과정에서의 응집을 방지하기 위한 분산제로 HPC를 첨가하는 방법에 의하여 구형의 다공성 알루미나 미분체를 제조하였으며, XRD, FT-IR 및 TG-DTA 분석을 통하여 공정변수로 선정한 물과 알루미늄 알콕시드의 비가 제조된 미분체의 결정화 공정에 미치는 영향에 대하여 고찰하였다. 축중합 반응에 첨가된 물과 Al-알콕시드의 비는 제조된 미분체 및 하소 공정을 거친 미분체의 결정구조에 영향을 미침을 알 수 있었다. [H₂O]/[ASB]의 비가 증가할수록 제조된 미분체는 비정질의 Al(OH)₃로부터 점진적으로 AlO(OH)의 화학조성을 갖는 의사-보헤마이트로 결정성이 바뀌었다. 그리고 건조 공정을 거친 미분체의 결정성이 좋을수록 δ와 θ-Al₂O₃는 저온에서 생성되었으나, α-Al₂O₃는 고온에서 생성되었다. 이와 같은 결과는 Al^(3+)이온의 결정 내에서의 배위체 변화와 관계됨을 알 수 있었다. 그리고 [H₂O]/[ASB]=2의 조건에서 제조된 입자는 다른 시료와 달리 600℃에서 η-Al₂O₃로 1,100℃에서 α-Al₂O₃2로 상전이 되었다. Spherical porous alumina particles were prepared by mixed solvent to control the reaction rate of Al-alkoxide and the particle shape with hydroxy-propyl cellulose(HPC). HPC was used as a dispersant to prevent aggregation during the formation of particles. The effect of [H₂O]/[ASB] ratio on the crystallization reactions of as-prepared particles was studied by XRD, FT-IR, and TG-DTA analysis. The mole ratio of water and Al-alkoxide added in the condensation reaction had an important effect on the crystal structure of as-prepared particles and the calcined particles. As the [H₂O]/[ASB] ratio increase, the crystal structure of as-prepared particles was continuously varied from amorphous Al(OH)₃ to pseudo-boehmite with chemical composition of AIO(OH). The samples which have higher crystallinite after drying process were transformed into the δ- and θ-Al₂O₃ at lower calcination temperature, but these sample were transformed into α-Al₂O₃ at higher calcination temperature. This result was thought to be the transformation of the coordination number of Al^(3+) ion in the crystal structure. The particles prepared by [H₂O]/[ASB]=2 condition crystallized to α-Al₂O₃at 600℃ and converted to η-Al₂O₃at 1,100℃ without intermediate phases.

Hall 소자를 이용한 승강기용 로프의 평가

이종오,윤운하,손영호,김정우,이종규 한국비파괴검사학회 2003 학술대회 논문집 Vol.- No.1

홀 소자를 이용한 누설자속 측정으로 승강기용 와이어 로프의 결함 검출에 대한 연구를 수행하였다. 소선파단, 마모, 내부 소선파단 형태의 인공결함을 제작하여 시험편에 영구자석으로 자장을 걸어 결함에서 누설되는 누설자속을 홀 센서를 사용하여 검출하였다. 로프의 소선은 0.5, 0.8mm 두 종류로 시험결과 단선모델 시험에서 0.4mm, 내부의 소선 파단의 경우 1mm 정도 깊이를 가진 인공결함까지 검출이 가능하여 1개의 소선 파단도 검출할 수 있는 것으로 보인다. 마모모델에서 결함의 폭이 작은 경우 검출이 불가능하였으나, 폭이 4mm이상인 경우 자극이 결함을 통과할 때 자속의 변화에 의해 깊이가 0.2mm 정도의 작은 결함도 구별이 가능하였다. 따라서 Hall 센서에 의한 누설자속 측정이 wire rope의 결함 검출에 좋은 도구가 된다고 본다. Defect detection of wire rope for an elevator was investigated through the measurement of magnetic flux leakage. The types of defect usually found in wire rope categorized such as inner and outer wire breakage and wear. The specimens that has artificial defects were magnetized via permanent magnet, and measurement of magnetic flux leakage on the defects was performed with Hall sensor. In wire broken model, a defect smaller than 0.4mm and 1mm in depth on outer and inner wire rope, respectively, could be detected well. In wear model, smaller defect could not be detected clearly, however, appearance of changing of total magnetic flux during magnetic pole of the sensor passing through a defect 0.2mm in depth at 4mm or above width could make possible to detect it. From the results, the measurement via Hall sensor might be useful tool for defect detection of wire rope.

티타늄 레이저 용접부의 기계적 성질 평가

이중재,김길수,안승근,배태성,박찬운 大韓齒科器材學會 2003 대한치과재료학회지 Vol.30 No.2

This study was performed to examine the effects of laser output energy on the mechanical properties of laser-welded titanium joints. Titanium rods of 3 ㎜ in diameter were perpendicularly cut to 30 ㎜ in length for the laser welding. Two rods were fixed to each other with a jig and welded vertically using a Nd:YAG laser at output current of 210-270 A. The penetration depth of the laser beam against the titanium was measured with the changes of output current, pulse duration, and spot diameter. Mechanical tests included tensile testing and Vickers microhardness of the joints. Tensile testing was performed at the crosshead 00speed of 1.0 ㎜/min and a gauge length of 30 ㎜ with a universal testing machine. Vickers microhardness was mesured under 9.8 N load for 15 seconds. The results obtained were summarized as follows; With increasing the output current, the penetration depth of the laser to titanium was increased. Tensile strength values of laser-welded groups were the relatively higher than that of non-welded parent material group. There were significant differences in tensile strength between the specimens of laser-welded at output currents 250 A, 270 A and non-welded parent material group(p<0.05). Microhardness values were increased in the heat-affected zone. Brittle fracture regions were observed in the specimens of laser-welded at output currents 270 A.

Canine Distemper Virus 검출을 위한 정량 Real-time PCR

이승관,이창규,정운원,조경진,김현숙,강영대,서장훈 高麗大學校 倂設 保健大學 保健科學硏究所 2002 保健科學論集 Vol.28 No.2

Canine distemper virus(CDV) infection causes a fatal systemic disease in wide range of carnivore species, including domestic dogs. It has been a highly contagious disease of veterinary importance for centurise. Recently many researches show that qualitative RT-PCR for the detection of CDV was sensitive and specific method for the early and safe antemortem diagnosis of distemper by using whole blood regardless of clinical sign and pathological finding. Recently, results were correlated to clinical findings, anti-CDV neutralizing antibody titers, postmortem findings, and demonstration of CDV NP(nucleoprotein) antigen by immunohistochemistry. We have studied a quantitative real-time PCR assay for CDV in order to improve and broaden the clinical usefulness of RT-PCR assay. Total 37 samples including 9 clinically positive and 29 randomly selected ones have investigated. CDV RNA was extracted from whole blood. The isolated RNA was reverse-transcripted into cDNA followed by PCR amplification. The assay based on real-time PCR using SYBR Green I dye as quantitative method with a LightCycler. Calibration curves covered 30-3×10^(8) copies of CDV were created using cloned 240bp CDV cDNA of viral RNA. CDV was detected all in 8 positive samples whereas 8 in 29 randomly collected ones. The detection ranges of clinical CDV specimens were various from 3×10² to 3×10⁴ copies. Comparision of real-time PCR to conventional PCR showed equal sensitivity and specificity. But real-time PCR method has a merit of quantification as well as more rapid results than those of common RT-PCR. CDV can be accurately detected and quantified in the whole blood from the dog infected with CDV. This quantitative technique may improve the clinical utility of RT-PCR for the detection of CDV.