Differential Manifestation of Ocular Phenotypes in TALEN-mediated p19arf Knockout FVB/N and C57BL/6J mouse lines

Jin‑Sung Park,Joo‑Il Kim,Hyun‑Jin Lim,Soo‑Kyung Ryu,Euna Kwon,Kang‑Min Han,Ki‑Taek Nam,Han‑Woong Lee,Byeong‑Cheol Kang 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.

Conceptual Study for Tissue-Regenerative Biodegradable Magnesium Implant Integrated with Nitric Oxide-Releasing Nanofi bers

Jin‑Kyung Jeon,Hyunseon Seo,Jimin Park,Soo Ji Son,Yeong Rim Kim,Eun Shil Kim,Jong Woong Park,Woong‑Gyo Jung,Hojeong Jeon,Yu‑Chan Kim,Hyun‑Kwang Seok,Jae Ho Shin,Myoung‑Ryul Ok 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.4

The excessive initial corrosion rate of Mg is a critical limitation in the clinical application of biodegradable Mg implantsbecause the device loses its fi xation strength before the fractured bone heals. This study suggests a new approach to overcomethis hurdle by accelerating tissue regeneration instead of delaying the implant biodegradation. As angiogenesis is anessential process in early bone regeneration, a Mg implant coated with electrospun nanofi bers containing nitric oxide (NO),which physiologically promotes angiogenesis, is designed. The integrated device enables adjustable amounts of NO to bestored on the NO donor-conjugated nanofi ber coating, stably delivered, and released to the fractured bone tissue near theimplanted sites. An in vitro corrosion test reveals no adverse eff ect of the released NO on the corrosion behavior of the Mgimplant. Simultaneously, the optimal concentration level of NO released from the implant signifi cantly enhances tube networkformation of human umbilical vein endothelial cells without any cytotoxicity problem. This indicates that angiogenesis canbe accelerated by combining NO-releasing nanofi bers with a Mg implant. With its proven feasibility, the proposed approachcould be a novel solution for the initial stability problem of biodegradable Mg implants, leading to successful bone fi xation.

2차병원에서의 소아과 환자의 응급실 및 외래 이용에 관한 연구

김진희,배진호,전진호,문정웅 인제대학교 2006 仁濟醫學 Vol.27 No.-

Objectives : To analyze the characteristics of the pediatric patients who visited the emergency room and outpatient department of secondary hospital in order to properly guide pediatric patients to use emergency room and raise the efficiency Methods : The authors investigated and analyzed sex, age, visit day, visit time, length of stay, hospitalization and diagnostic entities of the pediatric patients who visited the emergency room and outpatient department of Wallace Memorial Baptist Hospital from January 2003 to December 2005 in a retroactive approach with their medical records. Results : For the three years, the number of total patients who visited the emergency room was 56,051 and among them, the number of pediatric patients was 7,490 (13.4%). The age group of 1 to 2 years was the largest with 2,488 (33.2%). The male to female ratio was 1.34:1. Sunday was the day of the week that the largest number of patienst visited with 2,375 (31.7%) and nearly half (48.4%) of the patients visited the emergency room during the weekend. The time when the most patient visited the emergency room was between 8 pm to 12 am. Regarding the length of stay, the highest number of patients (65.0%) stayed in the emergency room for on hour or less, and the average length of visit was 1.5 hours, which was rather short. Most patients (68.8%) left the hospital after taking simple physical examination and procedure(prescription). The most frequent diagnoses for the pediatric patients who visited the emergency room were acute pharyngitis (26.0%), acute gastroenteritis (20.7%), fever (6.6%). During the same period, the total number of pediatric patients who visited the outpatient department was 125,513. The age group of 3 to 5 was the largest with 36,041 (28.7%). The male to female ratio was 1.34:1. 6.0% of total outpatients required treatment in a hospital. Most frequent diagnoses of the outpatients were asthma (24.4%), pneumonia (12.0%), vaccination (8.7%), febrile convulsion (8.4%), acute pharyngitis (6.6%), acute gastroenteritis (5.8%). Conclusion : It is considered that a large number of pediatric patients still have used emergency room for treatment of simple and mild diseases instead of visiting outpatient department and/or primary hospital. Thus further efforts are required to properly guide pediatric patients to use emergency room and to raise the efficiency of their use.

사람, 집박쥐 및 마우스 파네트세포의 Lysozyme 면역반응성

정진웅,차정호,오수자,전정수 대한해부학회 1991 Anatomy & Cell Biology Vol.24 No.2

치과 기공사들에게서 나타나는 호흡기 증상과 이의 관련 요인

김웅철,오세윤,김지환,김진완,이준석,이가영,이세훈,유진호,김태석 高麗大學校 倂設 保健大學 保健科學硏究所 2001 保健科學論集 Vol.27 No.1

This study was carried out to investigate subjective respiratory symptoms and their attributable factors in Korean dental laboratory technicians. A questionnaire was mailed to randomly selected 1,900 dental laboratory technicians. Among them, 1,344 dental technicians completed the questionnaires and returmned them. Validity and reliability tests on the questionnaires were carried out before the main survey. To investigate risk factors on respiratory subjective symptoms, we performed analysis on distribution of characteristics, and then investigated univariate analysis and multiple logistic regression analysis. The prevalence rate of wheezing, cough and phlegm, and dyspnea was 4.8%, 33.9%, 54.9%, respectively. Statistically significant factors inducing subjective respiratory symptoms by multiple logistic regression analysis were sex, smoking, exercise, health examination, work hours a day, investment gas, investment dust, metal fume, resin vapor, and asbestos dust. Considering the results above, it seems that most subjective respiratory symptoms are related with health habits and occupational environmental-related factors. The result, consideration in mind, suggest that health promotion programs for smoking cessation, regular health examination, and physical exercise in health habit, and improvement on casting and resin work in dental technological environment be necessary.

민속마을의 상품화와 교유성 인식 : 한국민속촌과 낙안읍성의 비교 Comparison between Korean Folk Village and Nakaneupsung

정병웅,김진수 순천향대학교 교수학습개발센터 2005 순천향 사회과학연구 Vol.11 No.2

Tourism is one of the various ways to experience different life styles, and to be in touch with traditional life styles of an other country or territory. This kind of tourism became popular after the 1990’s, which was due to the disappearance of tradition and the peak of industrialization. A trend to pursue the tradition and the past is related with not only tourist demand, but also tourism policy. The development of cultural tourism is helpful for the local development because of a economic method and rectifying the structural and seasonal vulnerability of tourism. The main attractions of traditional things among the cultural tourism resources in the insights to understand ancestors’ life style. The linkage between tourism and traditional culture is for the reconstruction of the past in the present by reproduction. But, sometimes traditional facts are distorted in the process of reconstruction and qualitative factors which has to be continued are disregarded to keep short term profits. In the case of folk villages, which the whole village is a tourism product, has lost the historical explanation and attractivity as a tourism resource by excessive business-related facilities. As a result visitors who want to touch the past way of life can not truly experience it. Authenticity and pursuing the short term profits are directly opposed ideas. Therefore it is desirable to keep a balance with authenticity and pursuing business profit, to keep value of attraction and sustainable cultural tourism.

完全雇傭과 財政政策 : Keynes의 「一般理論」을 中心으로

朴雄鎭 釜山工業大學 1967 論文集 Vol.5 No.-

파종기별 논콩재배의 수량 및 생태적 특성

조진웅,이석영,강순경,김충수 충남대학교 농업과학연구소 2006 농업과학연구 Vol.33 No.2

This study was conducted on a commerce silt loam soil at paddy field in the southwestern Korea (36˚N lat). In the study of planting time, seed were hand planted at 24 May, 14 June and 5 July, and at 24 May, 14 June and 5 July in 2005, respectively. Two seedlings plants per hill were taken prior to V3 stage. Fertilizer was applied prior to plant at a rate of 3.0-3.0-3.4 g (N-P-K) per square meter. Experimental design was a randomized complete block in a split plot arrangement with three replications. Yield was significantly affected by different of the planting times and cultivars. The seed yield in planting time was the highest on late in May 24 but was the lowest on July 5. Also, the plant time significantly affected on increasing pod and seed number and seed weight. The days from emergence to flowering and maturity was reducing tendency with late planting time.

시각장애자용 문자인지 장치에 관한 연구

허웅,조병서,김진곤,함광근,민홍기 明知大學校 産業技術硏究所 1994 産業技術硏究所論文集 Vol.13 No.-

In this paper, we implemented the hangeul tactile recognition system for the Blind. The system is consist of hardware part and software part.The hardware part is consist of a scanner interface image processing system, a electro tactile output device inter and auser interface. The software system consist of pattern learning routine,image processing and character pattern output routine, file management and operation window routine. The 16 by 16 of return electrode is driven by a electro-tacttile device. The recognition experimention distinguished learning beforefrom learning after,after learning the recognition rate is higher than learning before. As a results of experimentation before and after learning,the later is superior to the former. Therefore,if learning is increased, we can take higher recognition rate.

충주시 여중생의 흡연 실태 조사

정진웅,이학수 충북대학교 연초연구소 2009 煙草硏究 Vol.22 No.-

본 연구는 충주지역 여중생의 흡연 실태를 파악하기 위하여 설문조사한 것이다. 설문 대상자 420명중 24.3%(102 명)가 흡연을 하고 있었고, 그 분포는 1학년보다 2, 3학년에서 많았다. 이들 흡연자의 31%는 초등학교 시절에, 37%는 중학교 2학년에 담배를 배운 것으로 나타났다. 흡연의 동기는 '호기심'이고, 주로 인적이 드문 공터나 놀이터에서 피운다고 답했다. 흡연량은 하루에 10 개피 이하였지만, 1갑 이상을 피우는 학생도 13.7%나 되었다. 그리고 결손가정의 자녀, 성적이 나쁜 학생, 흡연을 하는 친구를 둔 학생들이 흡연을 많이 하는 것으로 나타났다. The smoking actual state of girls' middle school was investigated with 420 girls' students in Chungju city in korea by the questionnaire. The 102 girls of 420 girls middle school(24.3%) were inveterate smoker and the higher year students were smoked more than first year student. A 31% of 102 girl smokers replied that they smoked from the primary school. And then they began to smoke because of the curiosity to the smoking act of others, and they enjoyed a cigarette at a dark open place or a tranquil playing space mainly. They are smoking less than 10 cigarettes a day but 13.7% of 102 girls are enjoying more than cigarettes 20 everyday. And the students of imperfect household, the poor girl students, and students smoker friends are smoking more.