Determination of Capsaicinoids in Foods Using Ultra High Performance Liquid Chromatography

Jaeho Ha,Hye-Young Seo,You-Shin Shim,Dong-Won Seo,Homoon Seog,Masahito Ito,Hiroaki Nakagawa 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.4

A sensitive, precise, and specific ultra high performance liquid chromatographic (u-HPLC) method was developed for the analysis of capsaicin in foods. The method validation parameters yielded good results, including linearity, precision, accuracy, and recovery. The u-HPLC separation was performed on a reversed column C18(particle size 2 μm, i.d. 2 mm, length 50 mm, followed by fluorescence detection-excitation 280 nm, emission 325nm). The recovery of capsaicin in gochujang was more than 91%, and the detection limit and lower determination limit of u-HPLC analysis were 0.054 and 0.163 μg/mL for capsaicin and 0.053 and 0.160 μg/mL for dihydrocapsaicin,respectively. The calibration graph for capsaicin and dihydrocapsaicin was linear from 0.2 to 10.0 μg/mL for u-HPLC analysis. The inter-day and intra-day precisions (relative standard deviations) were <5.21% for capsaicin and <9.79% for dihydrocapsaicin while the average recoveries obtained were quantitative 91.1-94.8% for capsaicin, 91.4-97.0% for dihydrocapsacin, indicating good accuracy of the u-HPLC method.

Is quantum capacitance in graphene a potential hurdle for device scaling?

Lee, Jaeho,Chung, Hyun-Jong,Seo, David H.,Lee, Jaehong,Shin, Hyungcheol,Seo, Sunae,Park, Seongjun,Hwang, Sungwoo,Kim, Kinam Springer-Verlag 2014 NANO RESEARCH Vol.7 No.4

지방자치단체 공무원의 데이터기반 행정 영향 요인에 대한 탐색적 연구 : 자치구 공무원의 인식조사를 토대로

서재호(Seo, Jaeho) 한국지방정부학회 2020 지방정부연구 Vol.23 No.4

이 논문은 데이터기반 행정이 정부 혁신에 주는 의미가 매우 크다는 것을 전제로 데이터기반 행정에 영향을 미치는 요인이 무엇인가를 분석하였다. 데이터기반 행정의 영향요인으로 문화적 요인, 기술적 요인, 인적 요인, 리더십 요인, 제도적 요인을 제시했다. 조사는 부산광역시 A 자치구 본청직원 전체를 대상으로 진행했다. 분석결과 다섯가지 영향요인 중 통계적으로 유의미한 요인은 문화적 요인, 기술적 요인, 제도 요인으로 확인되었다. 이를 토대로 데이터기반 행정의 도입과 정착을 위해 첫째, 행정과정에서 데이터를 중요하게 여기고 활용의 관점에서 데이터를 관리하는 조직원의 태도형성이 필요하다는 점, 둘째 조직 전체 차원에서 데이터를 공동 활용할 수 있는 플랫폼의 구축, IT 기술 도입이 이루어져야 한다는 것과, 끝으로 각 부처간 데이터 칸막이를 해소할 수 있는 협업장치와 법적 기반 조성이 필요하다는 시사점을 제시하였다. This paper explores the factors that influence data-based administration for government innovation. Cultural, technical, human, leadership, and institutional factors were suggested as influence factors of data-based administration. The survey was conducted with the entire headquarters staff of the A autonomous district of Busan. As a result of the analysis, statistically significant factors were identified as cultural factor, technical factor, and institutional factor. Based on the result this paper proposed three policy implications; first, it is necessary to form attitudes of employees who manage data in the process of administration, and second, to build a platform for joint utilization of data at the whole organization level, finally, to construct IT systems, the introduction of IT technology, and legal foundations to resolve data partitions problems between ministries and offices.

연속적인 사분 파장 편광 변환을 이용한 평탄형 빗살 스펙트럼의 연속적인 파장 변화에 관한 연구

서재호(Jaeho Seo),이용욱(Yong Wook Lee) 한국조명·전기설비학회 2024 조명·전기설비학회논문지 Vol.38 No.3

국가와 지방자치단체간 다층적 규제 개혁에 대한 연구

서재호(Seo, Jaeho) 서울행정학회 2014 한국사회와 행정연구 Vol.24 No.4

이 연구는 문화재보호법과 문화재보호조례의 역사문화환경 보존지역 보호 규제를 사례로 지역주민의 삶의 질에 영향을 미치는 지방자치단체의 규제가 다층적 규제의 특성에 있음을 제시하면서 다층적 규제의 문제점을 분석하고 이를 개선하기 위한 시사점을 제시함을 목적으로 하고 있다. 광역자치단체의 문화재보호조례를 분석한 결과 다층적 규제에 대해 다음의 문제점이 제시되었다. 첫째, 다층적 규제에서는 각 지방자치단체 주민별로 규제형평성 문제가 발생할 가능성이 있다. 둘째, 다층적 규제에서 상위의 중앙정부 규제와 지방자치단체의 규제가 연계되어 있지 않고 있다. 셋째, 기존의 규제관리를 위한 등록제도는 다층적 규제를 효과적으로 관리하고 있지 못하다. 넷째, 현재의 규제개혁관리체제는 중앙정부와 지방자치단체간 연계된 다층적 규제 개혁의 필요성을 그다지 많이 반영하고 있지 못하다. 이러한 문제를 개선하기 위해 본 논문은 다음의 다섯가지 시사점을 제시하였다. 첫째, 지방자치단체가 차등적인 규제가 도입되는 것을 막기 위해 상위 법령차원에서 지방자치단체의 조례에 규제내용을 위임할 때 표준화된 기준을 제시할 필요가 있다. 둘째, 현행 규제등록시스템을 개선하여 다층적 규제의 특성이 있음을 등록내용에 추가하여야 한다. 셋째, 법체계상 법률과 조례의 정합성 문제를 극복하기 위해 종합성 확보조치의 도입이 필요하다. 넷째, 다층적 규제개혁을 위한 정부간 규제개혁 체제가 미흡한 점을 개선하기 위해 다층적 규제개혁 협의체가 구성되어야 한다. 다섯째, 지방자치단체의 규제개혁위원회와 규제담당부서의 규제개혁 기능강화 및 활성화가 필요하다. This study examines the problems of multi-level regulation between central and local government, which impacts on the lives of citizens, through a case study of the regulation of historic-cultural protection zones of regional government. Four problems relating to multi-level regulation and its reform are identified. First, there were serious problems concerning equity of regulation between different local governments. Second, the system of regulatory reform between central and local government were not connected in a consistent manner. Third, the current regulations enrollment system did not manage multi-level regulation effectively. Fourth, the regulatory reform system did not reflect the need for changes at the level of local government. To respond to these problems, this study offers five policy recommendations: The development of standardized regulation criteria for cases when central government delegates the right to enact law to local government; the addition of multi-level regulation traits to the enrollment system; the consistency of central and local law must be ensured; support for organizational structures in the reform of multi-level regulations should be maintained; the organization and functions of the reform committee of local government should be strengthened.

Radiotherapy as an effective treatment modality for follicular lymphoma

Seo Hee Choi,Jaeho Cho,Jin Seok Kim,June-Won Cheong,Chang-Ok Suh 대한방사선종양학회 2015 Radiation Oncology Journal Vol.33 No.4

Purpose: Follicular lymphoma (FL) is an indolent non-Hodgkin’s lymphoma that is highly sensitive to radiotherapy (RT). However, the effectiveness of RT has not been well established. We reviewed our experiences to assess the role of RT for FL and analyze treatment results. Materials and Methods: Retrospective analysis was done on 29 patients who received first RT between January 2003 and August 2013. Of 23 early stage (stage I, II) patients, 16 received RT alone, four received chemotherapy followed by RT, two received RT postoperatively, and one received salvage RT for relapse after resection. Six advanced-stage (stage III, IV) patients received RT after chemotherapy: two received consolidation RT, three received salvage RT for residual lesions, and one received RT for progressive sites. Median RT dose was 30.6 Gy (range, 21.6 to 48.6 Gy). Median follow-up duration was 62 months (range, 6 to 141 months). Results: All patients showed complete response in the radiation field. Eight outfield relapses were reported. Seven patients received salvage treatment (three chemotherapy, four RT). Four patients showed excellent responses, especially to RT. Estimated 5-year and 10-year relapse-free survivals were 72% and 60%. In the RT-alone group, 5-year relapse-free survival was 74.5%. All advanced-stage patients were disease-free with 100% 5-year overall survival. Disease-specific death was noted in only one patient; four others died of other unrelated causes. No significant toxicity was reported. Conclusion: RT resulted in excellent treatment outcomes for all FL stages when used as a primary treatment modality for early stage or salvage-treatment modality for advanced-stage disease.

Detection of Corn Oil in Adulterated Sesame Oil by Chromatography and Carbon Isotope Analysis

Seo, Hye-Young,Ha, Jaeho,Shin, Dong-Bin,Shim, Sung-Lye,No, Ki-Mi,Kim, Kyong-Su,Lee, Kang-Bong,Han, Sang-Bae Wiley Blackwell (John WileySons) 2010 Journal of the American Oil Chemists' Society Vol.87 No.6

Strategy for Comprehensive Identification of Post-translational Modifications in Cellular Proteins, Including Low Abundant Modifications : Application to Glyceraldehyde-3-phosphate Dehydrogenase

Seo, Jawon,Jeong, Jaeho,Kim, Young Mee,Hwang, Narae,Paek, Eunok,Lee, Kong-Joo 이화여자대학교 약학연구소 2009 藥學硏究論文集 Vol.- No.19

Post-translational modifications (PTMs) play key roles in the regulation of biological functions of proteins. Although some progress has been made in identifying several PTMs using existing approaches involving a combination of affinity-based enrichment and mass spectrometric analysis, comprehensive identification of PTMs remains a challenging problem in proteomics because of the dynamic complexities of PTMs in vivo and their low abundance. We describe here a strategy for rapid, efficient, and comprehensive identification of PTMs occurring in biological processes in vivo. It involves a selectively excluded mass screening analysis (SEMSA) of unmodified peptides during liquid chromatography-electrospray ionization-quadrupole-time-of-flight tandem mass spectrometry (LC-ESI-q-TOF MS/MS) through replicated runs of a purified protein on two-dimensional gel. A precursor ion list of unmodified peptides with high mass intensities was obtained during the initial run followed by exclusion of these unmodified peptides in subsequent runs. The exclusion list can grow as long as replicate runs are iteratively performed. This enables the identifications of modified peptides with precursor ions of low intensities by MS/MS sequencing. Application of this approach in combination with the PTM search algorithm MOD^(i) to GAPDH protein in vivo modified by oxidative stress provides information on multiple protein modifications (19 types of modification on 42 sites) with >92% peptide coverage and the additional potential for finding novel modifications, such as transformation of Cys to Ser. On the basis of the information of precursor ion m/z, quantitative analysis of PTM was performed for identifying molecular changes in heterogeneous protein populations. Our results show that PTMs in mammalian systems in vivo are more complicated and heterogeneous than previously reported. We believe that this strategy has significant potential because it permits systematic characterization of multiple PTMs in functional proteomics.

The rapid determination of PAHs in foods using ultra high performance liquid chromatography

Jaeho Ha(하재호),Dongbin Shin(신동빈),Jin-Bong Hwang(황진봉),Hye-Young Seo(서혜영),Masahito Ito(이토 마사히토),Hiroaki Nakagawa(나카가와 히오아키) 한국분석과학회 2011 분석과학 Vol.24 No.4

Performance Analysis of a New Preamble-Based SNR Estimation Algorithm

SEO, Changwoo,CHO, Insik,PORTUGAL, Sherlie,PARK, Jaeho,PARK, Gihwan,JUNG, Taejin,HWANG, Intae,LIU, Huaping,YOU, Cheolwoo The Institute of Electronics, Information and Comm 2011 IEICE TRANSACTIONS ON COMMUNICATIONS - Vol.eb94 No.5

<P>In recent years, techniques such as multiple input multiple output (MIMO) and orthogonal frequency division multiplexing (OFDM) have been developed and combined in MIMO-OFDM systems to provide higher data rates. In addition, the system can be optimized by setting modulation and coding adaptively according to the channel conditions. The overall system performance depends on how accurately the system obtains the channel state information (CSI) and feeds it back to the transmitter. In this paper, we propose a signal-to-noise-ratio (SNR) estimation algorithm in which the preamble is known by both sides of the transceiver. Through simulations of several channel environments, we prove that our proposed algorithm is more accurate than traditional algorithms.</P>