해양에서 분리한 Lantobacillus sp. JK-8에 의한 질병원인 세균의 살균효과

천재우,마채우,오계헌 순천향대학교 기초과학연구소 2004 순천향자연과학연구 논문집 Vol.10 No.2

The purpose of this work was to investigate for killing effect of disease-causing bacteria by Lactobacillus sp. JK-8 isolated from marine environment. Initially, a bacterial culture, strain JK-8 was developed to grow no MRS media. The bacterium was identified as genus Lactobacillus on the basis of BIOLOG test, and designated as Lactobacillus sp. JK-8. The intial pH 7 of the cultures became to decrease pH 3.85 at the end of incubaction according to the growth of Lactobacillus sp. JK-8. The antibacterial activity using plate diffusion method against target bacteria was determined with 5-fold concentrate of cell-free culture supernatant. Excellent killing effect of target bacteria was achieved. The inhibition zone obtained with culture supernatant were in between 14 mm and 20 mm. Killing rate of pathogenic bacteria was examined with cell-free concentrated supernatants. All bacteria tested in this work completely killed within 3 hrs of incubaction.

토양에서 분리된 Acrylamide 분해 세균 JK-7의 분리 및 특성

천재우,오계현 순천향대학교 기초과학연구소 2003 순천향자연과학연구 논문집 Vol.9 No.2

The feasibility of using pure culture for acrylamide degradation, with the ultimate aim of application for biological treatment process, was explored. The present study reports on the test cultures which were developed to grow aerobically with acrylamide as the sole source carbon and nitrogen. A bacterial isolate, strain JK-7 was isolated from paddy soil samples, Strain JK-7 could degrade 50 mM acrylamide completely within 72 hours of incubation. Major intermediates resulting from acrylamide degradation were not detected with the HPLC methodology except acrylic acid which appeared to accumulate transiently in the growth medium. Initial pH 7.2 of the cultures became to increase 8.4 at the end of incubation. When JK-7 cells were grown at over 100 mM acrylamide, there was a pause of cell growth, resulting in a reduction in the rates of acrylamide degradation. Survival test revealed that cells exposed to low concentrations of acrylamide enable to the strain JK-7 to survive at a lethal concentration of 100 mM acrylamide. The relationships between the acrylamide degradation by JK-7 and several relevant physicochemical environmental parameters were examained. The effect of supplemented carbons(e.g., glucose, fructose, citrate, succinate) on the acrylamide degradation by the test culture of JK-7 was evaluared. The results indicated that the addition of carbons accelerated the bacterial growth and acrylamide degradation compared to in the absence of supplemented carbons. The effect of supplemented nitrogens on the degradation was monitored. Increasing concentrations of yeast extract resulted in higher growth yield, based on the turbidity measurement, and complete degradation of acrylamide. However, acrylamide degradation was essentially uninfluenced by the addition of (NH_(4))_(2)So_(4),NH_(4)Cl, or urea. The bacterium was identified as belonging to the genus Pseudomonas in the basis of use BIOLOG test, and designated as Pseudomonas sp. JK-7

지역보건 관련 소지역간 건장증진표 개발에 관한 연구

김춘배,고광욱,박재성,최헌 韓國保健敎育.健康增進學會 2003 보건교육건강증진학회지 Vol.20 No.1

Purpose: Although there is a lot of secondary data available for comparing community health status and planning health policies in terms of large area such as metropolitan cities or provinces, there is restricted data for establishing community health policies of the small areas such as towns, Gun(i.e.,districts), and Gu. Specifically, the problems of producing a valuable index for health promotion in small areas are three fo1d: First, there is not an appropriate index model for measuring a small community health status. Second, a large part of secondary data in the small areas has been produced in an irregular time interval. In addition, all valuable data can not be integrated without time consuming work. Thus this study tries to establish a health promotion index model for assisting community health promotion initiatives of local governments. Methods and materials: Literature review, community health specialist consultation and a questionnaire survey was performed. Result: Based on Dever's model, a prototype of health promotion indicators was proposed and modified by the community health specialists. 15 classification scheme of statistical yearbook reorganized into the six areas. Those six areas were comprised in 24 indicator class with 96 specific indicators. Through further modification processes by a questionnaire survey, we developed a health promotion indicator model that contains six areas with 23 indicator class encompassed by 87 specific indicators. Conclusions: This study proposed a model of health promotion indicator comprised in the six areas with 23 indicator classes for measuring small area health promotion status. However, more specific or additional data in human biology, environment and socioeconomic data is essential for producing a stronger model for health promotion measurement.

관절 내시경술에 의한 퇴행성 슬관절염의 치료

김동헌,장병춘,신규철,이재성 건국대학교 의과학연구소 1999 건국의과학학술지 Vol.9 No.-

The arthroscopic surgery is an increasingly accepted procedure for the treatment of symptomatic arthritic knee. Authors reviewed the operative results of arthroscopic surgery in 35 knee joints of 27 patients from 1993 to 1997. 1. The average age of patients was 64 years old and the primary diagnosis was degenerative osteoarthritis in all patients. 2. Preoperative symptomes and signs were pain(98%), tenderness(85%), swelling(85%), decreased range of motion(71%) and effusion(55%). 3. The operative procedures were irrigation and synovectomy(20%), abrasion arthroplasty(57%), partial menisectomy and abrasion arthroplasty(23%). 4. The symptoms and signs were improved postoperatively: according to Nine point scale, the good results were recorded at postoperative 6 months and follow-up 2 years in nearly all patients. 5. The complications were minimal. Authors concluded that arthroscopic surgery is considered as a reliable method in the treatment of degenerative osteoarthritis of the knee but not as a curable method, needing a long term observation.

급성심근경색증의 조기진단에 있어서 Myoglobin의 진단적 의의

이정헌,김종근,정병천,서강석,박정배,조용근,류재근,전재은,채성철,박의현 大韓應急醫學會 1999 대한응급의학회지 Vol.10 No.2

Background: Exact and early diagnosis of acute myocardial infarction (AMI) is essential for the subsequent routine management of this frequent cardiovascular disease. Currently, AMI has been diagnosed using the combination of the history, electrocardiogram(ECG), and biochemical markers of myocardial necrosis. At present, many biochemical markers are used to diagnose AMI. In this study, the predictive values of serum myoglobin and creatine kinase-MB(CK-MB) were compared in the emergency department. Methods: Fifty-four consecutive patients who presented within 12 hours from onset of chest pain of presumed cardiac origin were enrolled into the study. Patients with trauma or renal failure were excluded. The serial serum myoglobin and CK-MB levels were obtained prospectively at admission and 2, 4, 6, 12, 24 and 48 hours after admission. We compare the levels of serum myoglobin and CK-MB within 2, 4, 6, 8, 10, 12, 24, and 48 hours after symptom onset respectively. And we compare the sensitivity, specificity, positive predictive value, and negative predictive value of myoglobin and CK-MB. Results: using World Health Organization criteria, 28 AMI patients were identified. Mean time from symptom onset to presentation was 4.1±1.3 hours. The predictive values of serum myoglobin were better than those of CK-MB within 6 hours after symptom onset. But, 6 hours after symptom onset, the predictive values of CK-MB were better than those of serum myoglobin. The false positive cases of serum myoglobin were 3-one was lung cancer with pleural effusion and the others were unstable angina. The false positive cases of CK-MB were 6-one case was viral myocarditis and the ohters were unstable angina. Conclusion: To compare the serum myoglobin and CK-MB in the diagnosis of AMI, serum myoglobin had better predictive values than CK-MB within 6 hours after symptom onset and useful in the early diagnosis of AMI. But, 6 hours after symptom onset, CK-MB had better predictive values than serum myoglobin and useful in following up.

녹차추출물에 의한 식중독 원인균인 장내세균 Plesiomonas shigelloides의 세포반응

유미옥,천재우,오계헌 순천향대학교 기초과학연구소 2002 순천향자연과학연구 논문집 Vol.8 No.2

The aim of this work was to investigate the cellular responses in the food-poisoning bacterium, Plesimonas shigelloids in response to tea polyphenol (TPP) extracted from green tea. The stress shock proteins, which contribute to the resistance of the cytotoxic effect of TPP, were induced at different TPP concentrations in exponentially growing culture of P. shigelloides. This response involved the induction of a 70-kDa DnaK and a 60-kDa GroEL monoclonal antibodies, characterized by SDS-PAGE and Western blot by the use of the anti-DnaK and anti-GroEL monoclonal antibodies. Survival of P. shigelloides with time in the presence of different concentrations of TPP was monitored, and viable counts paralleled the induction of the stress shock proteins in this strain. This strain killed in the concentration of 1,000 ㎍/㎖ TPP within 2 hours of incubation. Scanning electron microscopy for the cells treated with 10,000 ㎍/㎖ for 12 hours showed the presence of perforations and irregular rod shaped with wrinkled surfaces.

Tea polyphenol에 노출된 식중독 원인세균 Listeria monocytogenes의 살균

유미옥,천재우,오계헌 순천향대학교 기초과학연구소 2004 순천향자연과학연구 논문집 Vol.10 No.2

The aim of this work was to investigate the killing effect of the food-poisoning bacterium, Listeria monocytogenes exposed by tea polyphenol (TPP) extracted from green tea. The stress shock proteins, which contribute to the resistance of the cytotoxic effect of TPP, were induced at different TPP concentration in exponentially growing cultures of L. monocygenes. This response involved the induction of a 70-kDa DnaK and a 60-kDa GroEL monoclonal antibodies, characterized by SDS-PAGE and Western blot by the use of the anti-DanK and anti-GroEL monoclonal antibodies. Survival of L.monocytogenes with time in the presence of different concentrations of TPP was monitored, and viable counts paralleled the induction of the stress shock proteins in this strain. This strain killed in the concentration of 1,000㎍/㎖ TPP within 4 hours of incubation. Scanning electron microscopy for the cells treated with 1,000㎍/㎖ for 12 hours showed the presence of perforations and irregular rod shaped with wrinkled surfaces.

Clinical and histological significance of atypical glandular cells on cervical cytology(초)

최홍준 ( Hong Jun Choi ),( Woo Chul Kim ),( Ho Soap Hahn ),( Seok Geun Yoon ),( Yong Soon Kwon ),( In Ho Lee ),( Kyung Taek Lim ),( Ki Heon Lee ),( Jae UK Shim ),( Jung Eun Mok ),( Yi Kyeong Chun2 ),( Ta 대한산부인과학회 2009 대한산부인과학회 학술대회 Vol.95 No.-

다목적실용위성 영상에 최적화된 Modified Ratio Pan-Sharpen 기법의 연구

정재헌(Jae-Heon Jeong),서두천(Doo-Chun Seo),이선구(Sun-Gu Lee),서동춘(Dong-Chun Seo) 대한공간정보학회 2014 한국지형공간정보학회 학술대회 Vol.2014 No.5

Validation and Reliability of a Smartphone Application for the International Prostate Symptom Score Questionnaire: A Randomized Repeated Measures Crossover Study

Kim, Jae Heon,Kwon, Soon-Sun,Shim, Sung Ryul,Sun, Hwa Yeon,Ko, Young Myoung,Chun, Dong-Il,Yang, Won Jae,Song, Yun Seob JMIR Publications Inc. 2014 Journal of medical Internet research Vol.16 No.2

<P><B>Background</B></P><P>Smartphone-based assessment may be a useful diagnostic and monitoring tool for patients. There have been many attempts to create a smartphone diagnostic tool for clinical use in various medical fields but few have demonstrated scientific validity.</P><P><B>Objective</B></P><P>The purpose of this study was to develop a smartphone application of the International Prostate Symptom Score (IPSS) and to demonstrate its validity and reliability.</P><P><B>Methods</B></P><P>From June 2012 to May 2013, a total of 1581 male participants (≥40 years old), with or without lower urinary tract symptoms (LUTS), visited our urology clinic via the health improvement center at Soonchunhyang University Hospital (Republic of Korea) and were enrolled in this study. A randomized repeated measures crossover design was employed using a smartphone application of the IPSS and the conventional paper form of the IPSS. Paired <I>t</I> test under a hypothesis of non-inferior trial was conducted. For the reliability test, the intraclass correlation coefficient (ICC) was measured.</P><P><B>Results</B></P><P>The total score of the IPSS (<I>P</I>=.289) and each item of the IPSS (<I>P</I>=.157-1.000) showed no differences between the paper version and the smartphone version of the IPSS. The mild, moderate, and severe LUTS groups showed no differences between the two versions of the IPSS. A significant correlation was noted in the total group (ICC=.935, <I>P</I><.001). The mild, moderate, and severe LUTS groups also showed significant correlations (ICC=.616, .549, and .548 respectively, all <I>P</I><.001).There was selection bias in this study, as only participants who had smartphones could participate.</P><P><B>Conclusions</B></P><P>The validity and reliability of the smartphone application version were comparable to the conventional paper version of the IPSS. The smartphone application of the IPSS could be an effective method for measuring lower urinary tract symptoms.</P>