Genome-wide investigation of a Korean synthetic breed, Woori-Heukdon using the Illumina PorcineSNP60K BeadChip

Yong‑Min Kim,Ha‑Seung Seong,Jung‑Jae Lee,Da‑Hye Son,Jin‑Su Kim,Soo‑Jin Sa,Young‑Sin Kim,Tae‑Jeong Choi,Kyu‑Ho Cho,Joon‑Ki Hong,Jung‑Woo Choi,Eun‑Seok Cho 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: Woori-Heukdon (KWH) is a Korean synthetic pig breed generated using Chookjin-Duroc (KCD), Chookjin-Chamdon (KCC), and their crossbreds. Currently, there is a severe lack of studies investigating the Korean breed populations including wild boars (KWB) throughout the genome. Objective: This study was performed to investigate the genetic characteristics of Korean pig populations at the genome-wide level. Methods: Using the SNP dataset derived from genotyped and downloaded datasets using the Illumina PorcineSNP60K BeadChip, we compared the genomes of 532 individuals derived from 23 pig breeds to assess the genetic diversity, inbreeding coefficient, genetic differentiation, and population structure. Results: KWB showed the lowest average expected heterozygosity (HE = 0.1904), while KWH showed the highest genetic diversity (HE = 0.02859) among Korean populations. We verified that the genetic composition of KWH, showing USD of 74.8% and KCC of 25.2% in ADMIXTURE analysis. In population structure analyses, KCC was consistently shown to be separated from other pig populations. In addition, we observed gene flow from Western pigs to a part of Chinese populations. Conclusion: This study showed that Korean native pigs, KCC have genetic differences in comparison with Chinese and Western pigs; despite some historical records and recent genetic studies, we could not find any clear evidence that KCC was significantly influenced by Chinese or Western breeds in this study. We also verified the theoretical genomic composition of KWH at the molecular level in structure analyses. To our knowledge, this is the first genomic study to investigate the genomic characteristics of KWH and KCC.

소설의 재매개와 포스트휴먼의 형성-소설 ≪천룡팔부(天龍八部)≫에서 게임 ≪천룡팔부(天龍八部)3D≫까지-

최재용 ( Choi Jae-yong ) 한국중국어문학회 2017 中國文學 Vol.90 No.-

본고는 소설 《天龍八部》가 디지털 게임 《天龍八部3D》로 재매개(remediate)되는 과정을 추적하고, 그 과정 중에서 나타나는 미디어 판본의 차이를 밝힌다. 나아가 그 과정 속에서 새로운 주체, 즉 포스트휴먼이 등장하고 있다고 주장한다. This study starts with Jin Yong`s famous novel, < Demi-Gods and Semi-Devils >, or < Tianlongbabu >. But what I mainly discussed is not the novel itself, but a mobile game < Tianlongbabu3D >, which is based on the novel. I tried to find out what happened during the process of translating the novel into the game, and to explain what it means. One of my two main concept is `remediation`. When a new media emerges, people tend to think that this new media is going to completely replace the old one. But the invention of camera did not extinguish painters, and kindle did not replace paperback. In fact, picture and painting, e-book and actual book created a complex interaction, affecting each other. We call this interaction `remediation`. When a novel < Tianlongbabu > was transformed into a mobile game < Tianlongbabu3D >, this `remediation` happened. The game looks like it`s telling the same story as the novel did, but different media has different characteristic. For instance, in the game you can visit the same place as many times as you want, and beat enemies you couldn`t dream of beating in the novel. Next main concept is `posthuman`. By posthuman, I mean the change of the very concept of human being. To humanism subject, reason is very important. They have very clear `boundary`, which consists their `self`. But posthuman transcends above this boundary. They embrace `alien` things like metal arm or electric body parts, they do not exclude things that were considered to be outside of the boundary, like cyborg, robot or A.I.. I think what we see in the game < Tianlongbabu3D > is the emergence of posthuman. The playable character that the player controls is posthuman, and the player itself is also posthuman. Above the boundary of human/smartphone, the posthuman ascends. In short, through this study I found out that the remediation of novel by game produces new meanings and activities, and that the very concept of human self is shaking. Posthuman is already among us.

우심방을 침범한 거대 비호지킨씨 림프종 1예

강상범,진승원,이은경,박용현,최용호,김용주,박준철,김재형,홍순조,최규보,권종범,원용순,박건,이은희 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.4

Richard Meier의 건축특성에 관한 연구

이용재,최영재,문영삼 순천향대학교 산업기술연구소 2006 순천향 산업기술연구소논문집 Vol.12 No.1

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Candida tropicalis로 부터 분리된 자율복제기점을 이용한 Candida maltosa와 Saccharomyces cervisiae에서의 shuttle-vector의 구축

최용락,조영수,차재영 東亞大學校附設遺傳工學硏究所 1994 遺傳工學硏究 Vol.- No.1

무포자 효모인 Candida속의 효모는 aikane계 화합물을 이용하며 cellubiose나 xylose를 동화하는 등 산업적으로 이용가능성이 높다. Candida tropicalis는 세포질 인자인 plasmid가 발견되지 않으므로 염색체 유래의 자율복제기점을 non-replicative plasmid인 YIp5에 삽입시켜 S. cevisiae YNN 27에서 분리하였다. 분리된 자율 복제 기점을 Hind III과 EcoR I제한 효소로 절단하고 각각 2.7kb와 2.3kb fragment를 회수한 후 동일 제한 효소로 절단한 YIp5와 YIp32에 ligation시켜 pIKS523, pIKS527및 pIKC27을 구축하였다. 특히, pIKC27은 2.7kb의 자율복제 기점을 가진 plasmid로서 S. cerevisiae DBY746과 C.maltosa J288에서 높은 형질 전환체를 나타내었다. 기존의 plasmid와 안정성을 비교하였으며, 여러 제한효소에 의해서 얻어진 결과로서 상세한 restrition map을 작성하였다. Candida species are special interest because of its ability to metabolize hydrocarbon, covet this material into single cell protein and assimilate cellobiose and xylose as substrate. Cadida tropicalis has no native plasmid similar to 2㎛ plasmid. Therefore, Candida tropicalis is regarded as a new yeast host. An 8-kb fragment was isolated from Sal I digest of Candida tropicalis IFO 0518 genomic DNA which conferred the property of autonomous replication in Saccharomyces cerevisiae YNN 27. The vectors for gene manipulation of Yeast, pIKS 523, pIKS 527 and pIKC 27, were constructed by combinding the ARS fragment and integration plasmid YIP 5 and YIP 32, respectively. One of the recombinant plasmids, pIKC 27 (9.4kb), was capable of autonomous replication in both S. cerevisiae DBY 746 to Leu+ at a frequency of 600 transformants per ㎍ DNA, and transformed Candida maltosa J288 to Leu+ at a frequency of 230 transformants per ㎍ DNA. These results indicate that the 2.7 kb ARS element was necessary for high frequency transformation and autonomous plasmid replication in both S. Cerevisiae and C. maltosa.

북한 고등중학교 「지리」 교과서 분석 연구

최석진,남상준,류재명,손용택,이동엽 한국사회과교육연구회 2000 사회과교육 Vol.- No.33

이 연구는 남북한 통일을 대비하여 북한 고등중학교 지리 교육과정 및 교과서를 비교·분석한 것이다. 남북한의 학제와 지리과 영역의 교육과정상의 위치가 다르고 북한의 교육과정을 입수할 수 없었기 때문에, 입수한 북한의 고등중학교 1학년부터 5학년의 지리교과서 5종(1991년도부터 1993년도 발행분)을 대상으로 분석하여, 남한 6차 교육과정의 중학교 사회와 고등학교 공통사회(한국지리) 및 세계지리 내용을 비교 분석하였다. 연구 결과는 다음과 같다. 첫째, 남북한 중등학교 지리교육은 교육과정상의 편제에서 북한은 고등중학교 전학년에 걸쳐서 지리가 독립과목으로 편제되어 있으나, 남한은 중학교 사회과에 통합되어 있고, 고등학교에서는 공통사회에 포함되었으며 세계지리가 선택과목으로 되어있는 등 지리교육 내용계열과 조직체계도 차이가 난다. 둘째, 지리 교육의 이념과 목표에서, 남한은 민주시민 양성을 목표로 하여 개인의 지리교육적 성취가 주된 것이나, 북한은 집단 구성원으로서의 개인 양성에 중점을 두고 있다. 이러한 차이점은 정치체제의 차이에서 비롯된 것이며 지리교육 내용의 구성과 강조점등에서 많은 차이점을 나타내게 하고 있다. 셋째, 지리교육 내용 구성과 서술 등에 차이가 있다. 국토지리와 세계지리 및 계통지리와 지지의 배열과 비중 등이 다르며, 내용의 서술에서도 차이점을 볼 수 있다. 특히, 북한에서는 국토지리와 자연지리의 비중이 높으며, 주요 개념과 용어 설명에서 남한과 다른 점이 보인다. 넷째, 교과서의 구성과 교수-학습 방법 및 자료 사용에서 차이점이 보인다. 북한에서는 실습, 실험 등이 강조되면서 실제적인 기능 향상에 많은 비중을 두고 있으며, 사진에 비해서 그림을 사용하는 삽화의 비중이 높다. 교과서의 외형적 체제와 지질 등에서 차이가 큰데, 이 점은 경제력의 차이에서 비롯된다. 본 연구 결과 남북한은 모두 지리교육을 중등학교에서 중요시하고 있으며, 지리교육을 통하여 소기의 목표 달성에 노력하고 있으나, 서로간에 상당한 차이점도 발견할 수 있었다. The purpose of this study is to understand the similarity and differences of the geography textbook between north and south Korea in preparation for the unification. Five geography textbooks for the first through fifth grade in secondary school were used in the analysis of the geography curriculum for secondary schools in North Korea. The major findings of the study are as follows: First, South Korea takes an integration approach to teach geography as part of the social studies education whereas North Korea treats geography as an independent subject throughout secondary school. Second, South Korea aims at nurturing democratic citizen, emphasizing individual achievement, whereas North Korea puts emphasize nurturing citizenship within community. Third, difference is noted in the organization and content of the geography curriculum between the two Koreas. North Korea puts more emphasis on national geography and natural geography than does South Korea. Forth, North Korean textbook is different from that of South Korea in that the former emphasizes on performance skills through hands-on practice and training. Also North Korean textbook uses more drawings than pictures. Despite differences in various aspects of curriculum and textbooks, there are a great deal of overlap in essential concepts and contents covered in two Koreas' geography textbooks. Both Koreas value geography education in the secondary schools and make efforts to fulfil the educational objectives in geography.

팥의 RAPD 분석 조건 최적화 연구

이충열,박현철,박진철,김성만,김용철,최재희,최인수 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

The object of this study was to optimize PCR condition for RAPD analysis in adzuki bean. The best template DNA concentration was 20ng(0.25unit taq polymerase and 2.5mM MgCl2), 40ng(1unit taq polymerase and 2.5mM MgCl2, 1unit taq polymerase and 4.5mM MgCl2 and lunit taq polymerase and 7.0mM MgCl2), and 60ng(0.5unit taq polymerase and 2.5mM MgCl2, 0.5unit taq polymerase and 4.5mM MgCl2, 1unit taq polymerase and 2.5mM MgCl2, and 1unit taq polymerase and 4.5mM MgCl2) The best MgCl2 concentration was 2.5mM(40ng template DNA and 1unit taq polymerase, 60ng template DNA and 0.5unit taq polymerase, and 60ng template DNA and 1unit taq polymerase), 4.5mM(20ng template DNA and 0.25unit taq polymerase, 60ng template DNA and 0.5unit taq polymerase, and 60ng template DNA and 1unit taq polymerase), and 7.0mM(40ng template DNA and 1unit taq polymerase). Amount of taq polymerase was 0.25unit(20ng template DNA and 2.5mM MgCl2), 0.5unit(60ng template DNA and 2.5mM MgCl2, 60ng template DNA and 4.5mM MgCl2) and 1unit(40ng template DNA and 2.5mM MgCl2, 40ng template DNA and 4.5mM MgCl2, 40ng template DNA and 7.0mM MgCl2, 60ng template DNA and 2.5mM MgCl2, 60ng template DNA and 4.5mM MgCl2, and 60ng template DNA and 7.0mM MgCl2). When we consider results from template DNA concentration, MgCl2 concentration, and amount of taq polymerase, the best condition for PCR optimization was 60ng template DNA, 4.5mM MgCl2, and 1unit taq polymerase. Reaction temperatures for the optimal PCR condition were 84℃, 32℃, 62℃; 90℃, 40℃, 72℃; and 92℃, 36℃, 72℃.

참깨에 對한 Ethephon 및 Choline Salt of Maleic Hydrazide(CMH) 處理가 主要形質에 미치는 影響

崔鎭景,金容在 全南大學校 農漁村開發硏究所 1991 農業科學技術硏究 Vol.26 No.-

This study was conducted to improve the percentage of rippeness and increase the yield by elimination of ineffective capsules and withering of growing poiont with spraying the proper concentration of the Ethephon and the CMH at a suitable application time during late growth stage of sesame. The sesame which was used experiment was Danbaek sesame. The Ethephon and the CMH were treated with 250, 500, 1000 and 2500 ppm, and 1000, 2500, 5000 and 10000 ppm on 20, 25, 30, 35 days after flowering time respectively. 1. The stem length was regulated by the two regulators. The effect of Ethephon at early growth stage was higher than at late growth stage. 2. At least, minimum capsules per plant were obtained when the Ethephon and the CMH were applied to below 250 ppm and 5000 ppm on 30 days after flowering time respectively. 3. The percentage of rippeness inferred which was improved by treatment the Ethephon on 25 to 30 days after flowering time. The CMH was increased the percentage of rippeness at all treatment except 10000 ppm. If it were considerated the percentage of rippeness and side capsule, it would moderate applying 250 ppm on 30 days after flowering time. The effects of CMH were higher than those of ethephon. 4. Concentration of the Ethethon and the CMH which didn't lead to decreasing percentage of rippeness were 500 ppm and below 2500 ppm respectively. 5. When the Ethephon was applied to sesame plant, chlorophyl contents of sesame plant arrived to peak on 4 days after application. The other hand the CMH was decreased chlorophyl contents 33 to 48 percent. 6. The both growth regulators didn't affect on the emergency ratio of sesame, but the CMH was decreased 90 percent the emergency ratio of sesame when it was treated more than 20000 ppm. 7. Form finishing flower to maturing stage, the term was obtained by the both growth regulator was 19 to 22 days, 15 to 18 days, 9 to 13 day and 4 to 7 day treatment 20, 25, 30 and 35 days after flowering time respectively. For this period, assimilating products would move plant into grain. 8. Two regulators lead to decreasing no. of grains in capsules formed when those were applied to. 9. When the ethephon was treated on sesame, the percentage of rippeness was improved, but it was difficult to form full capsules per plant. Thus, it didn't expect to increase yield of sesame. While, when the CMH was treated with 2500 ppm on 30 days after flowering time. Because it was subject to obtaining full capsules per plant and was improved the percentage of rippeness. The yield of sesame would be increased 12% than that (85kg/ 10a) of control.

임신 중기 급성 A형 간염의 치료 1예

김미영,문성택,김윤숙,전섭,최승도,선우재근,배동한 순천향의학연구소 2007 Journal of Soonchunhyang Medical Science Vol.13 No.2

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일부 보건소 내소 여성의 유방자가검진과 유방암 검진의 관련요인

최종숙,박재용,한창현 韓國保健敎育.健康增進學會 2001 보건교육건강증진학회지 Vol.18 No.1

The purpose of this research was to find out plans to promote the fulfillment of the breast self-examination and breast cancer screening, which is preventive health behavior about women's having breast cancer exam in some part of our country with searching for the primary causes which have an effect on their fulfillment. The subjects of the study were 540 literate volunteers who were of age from 20 to 69 and who visited 8 health centers in Taegu. The survey research was made through questionares in the 8 health centers in Taegu from the 15th to the 30th of September, 1998. Based on Becker's health belief model asking medical staff's opinions with bibliographies, the major findining from the survey was as follow. The fulfillment rate of the breast self-examination and the breast cancer screening was 37.8%(204 subjects), 27.6% (149 subjects) each. Getting breast self-exam varied significantly depending on subject's age, income of family, religion, occupation and the number of children. As far as the breast cancer screening was concerned, there was remarkably significant difference in accordance with their age, marital status, religion, occupation, income of family, the number of children, ways of nursing and parturition age of the first baby. The subjects in their forties accounted for the highest rate. Based on the fulfillment of the breast self-examination and the breast cancer screening, the average points of all the variables presented from the health belief as well as the points of the susceptibility, seriousness, benefits, knowledge and level of health concern of interest in health were significantly higher than the points of non-fulfillment of them in the statistical analysis whereas the barriers was lower than them. In consequence of the multiple-logistic regression analysis putting the breast self-examination and the breast cancer screening in dependent variables, occupation, knowledge, seriousness, benefits, barriers and cues to action had significant effects on breast self-examination against breast cancer. Looking more attainments, thinking diseases more seriously, feeling more usefulness and there being any patient around with breast cancer led to the higher rate of the breast self-examination against it. On the other hand, feeling more impedition as well as housewives caused the lower rate of it. The breast cancer screening was significantly affected by age, sensitivity, usefulness, impedition and behavior momentum as well. The cases who are older, feeling more sensitivity against breast cancer, more usefulness of the examination and where any patient around, the fulfillment rate was higher. Contrarily the more impedition they felt, the lower the fulfillment rate was. Accordingly, the study of the causes, which have significant effects on the fulfillment of the self-examination and the breast cancer screening in order to find it in an early stage, and the development of health education programs as well as proper education for public health through carrying on campaigns and stepping up public relations must be indispensable.