임플란트의 표면처리 유형에 따른 골 치유 양상

고영한,김영준,정현주 전남대학교 치과대학 2001 구강과학 Vol.13 No.1

It is well known that the apposition of bone at implant surface would be influenced by the microstructure of titanium implants. The purpose of this study was to compare bone healing around the screw­shaped titanium implant with three different surface topographies in the canine mandibles by histological and biomechanical evaluation. All mandibular premolars of six mongrel dogs were extracted and implants were placed one month later. The pure titanium implants had different surface topographies: smooth and machined (Steri­Oss^ⓡL Group Ⅰ): acid­etched (Osseotite^ⓡ: Group Ⅱ); sandblasted and acid­etched (ITI^ⓡ, SLA: GroupⅢ) surface. The fluorescent dyes were injected on the 2nd (calcein), 4th (oxytetracycline HCI) and 12th (alizarin red) weeks of healing Dogs were sacrificed at 4 and 12 weeks after implantation. The decalcified and undecalcified specimens were prepared for histological and histometrical evaluation of implant­bone contact. Some specimens at 12 weeks after implantation were used for removal torque testing. Histologically, direct bone apposition to implant surface was found in all of the treated groups. More mature and dense bone was observed at the implant ­ bone interface at 12 weeks than that at 4 weeks after implantation. Under the fluorescent microscope, thick regular green fluorescent lines which mean early bone apposition were observed at the implant ­ bone interface in Group Ⅲ, while yellow and red fluorescent areas were found at the implant ­ bone interface in Group Ⅰ and Ⅱ. The average implant ­ bone contact ratios at 4 weeks of healing were 54.3% in Group Ⅰ, 57.7% in Group Ⅱ and 62.2% in Group Ⅲ. In Group Ⅰ, implant ­ bone contact ratio was significantly lower than Group Ⅱ and Ⅱ (p<0.05). The average implant ­ to ­ bone contact ratios at 12 weeks after implantation were 64.3% in Group Ⅰ, 66.7% in GroupⅡ, and 71.2% in GroupⅢ. There was no significant difference among the three groups. In Group Ⅰ andⅡ, the implant ­ bone contact ratio at 12 weeks increased significantly in comparison to ratio at 4 weeks (p<0.05). The removal torque values at 12 weeks after implantation were 90.9 N㎝ in Group Ⅰ, 81.6 N㎝ in GroupⅡ and 77.1N㎝ in GroupⅢ, which were significantly different (p<0.05). These results suggest that bone healing begin earlier and be better around the surface ­ treated implants compared to the smooth surface implants. The sandblasted and acid ­ etched implants showed the most favorable bone response among the three groups during the early healing stage and could reduce the waiting period prior to implant loading.

대퇴골 전자간 골절을 동반한 고령의 환자에서 시행한 슬관절 전치환술

고덕환,김동헌,김규현,신주용,양준호 건국대학교 의과학연구소 2001 건국의과학학술지 Vol.11 No.-

Purpose: To analyze the clinical results of total knee arthroplasty In elderly patients who had arthritic knees and intertrochanteric fractures. Materials and Methods: From fan 1992 to March 1999, 27 cases of the patients with arthritic knees and associated intertrochanteric fractures were treated by total knee arthroplasties and we evaluated the patients by Knee Score of American Knee Society. Results: Flexion contracture was improved from 10.3 degree to 5.2 degree, KSS score was improved from 57.4 points to 78.6 points, authors' evaluation system score was improved from 50.3 points to 73.2 points. Conclusion: In elderly patients with arthritic knees and associated intertrochanteric fractures, total knee arthroplasty seemed to decrease the rehabilitation time and allow earl weight bearing, so provide an improvement in quality of life.

正常 Rat에서의 蔗糖經口投與에 依한 高血糖症에 미치는 대추나무 葉 抽出物의 影響

고현철,서대규,김병수,강주섭 漢陽大學校 環境科學硏究所 1994 環境科學論文集 Vol.15 No.-

대추나무의 열매는 漢方에서 여러方面에 廣範이 利用되고 있으나 그 잎은 利用되지 않고 있으나 이 잎에는 甘味受容抑制用과 糖의 腸에서의 吸收抑制作用을 가지는 成分이 含有되어 있음이 밝혀져 糖吸收抑制物質로서의 開發의 對象이 될 수 있을 것이다. Insulin??乏等으로 因하여 耐糖能에 異常이 있는 疾患에서는 糖吸收을 抑制함으로 腸管에서의 急擊한 大量의 糖의 吸收를 事前에 防上시켜 Insulin分泌細胞에 대한 負荷를 輕減시켜 줄 수 있는 것임으로 대추나무 葉 抽出物이 糖吸收抑制作用이 있는지를 確認코져 蔗糖負荷動物에서의 血糖値 上昇反應에 미치는 대추나무 葉 抽出物이 作用을 檢討하여 다음과 같은 成績을 얻을 수 있었다. 1. 대추나무 葉 抽出物이 負荷된 蔗糖量의 1/10에 該當되는 比較的 少量에서는 蔗糖負荷에 依한 血糖値上昇作用을 抑制할 수 없었다. 2. 比較的 大量 卽 蔗糖負荷量의 3/10에 該當되는 用量에서는 대추나무 葉 抽出物이 蔗糖負荷에 依한 血糖値上昇을 有意하게 抑制하였다. 以上의 結果로 미루어 보아 대추나무 葉 抽出物은 糖攝取에 의한 血糖値上昇反應을 억제하여 비만과 당뇨병치료에 이용될 수 있을 것으로 사료된다. The fruits of the Ziziphus jujuba have been to use an application in the various ways of chinese medicine and the leaves of this plant have been not to use for this purpose. But, It was pointed out that leaves of the Ziziphus jujuba contain the ingredients of inhibitory action in sweet taste reception of the tongue and intestinal absorption of glucose. Therefore, it is done to developing substance as inhibitory substance of intestinal glucose absorption. The patients with glucose intolerance because of insulin deficiency have a condition of a sudden intestinal glucose absorption. For this reason, the suppression of the sudden glucose absorption alleviate loads of insulin secretion of pancreatic islet cell. In this study, the author have the purpose which confirms the effect of EZJ to intestinal glucose absorption by sugar intake with this substance, that is, levels of blood glucose after sucrose in dose of 6g/kg of body with EZJ in dose of 0.6g/kg of weight were determined to investigate the effect of EZJ on the elevation of blood glucose levels with sugar intake in normal rats. After an overnight fasting, sucrose solution was given orally to the control group. Experimental groups were divided into single EZJ, Triple EZJ, EZJ administered at 2 hours and 1 hours before sucrose and another one with sucrose. Blood glucose levels were determined with Glucoscott at just before and 15, 30, 60, 90 and 120 minutes after sucrose adminstration. The results were as follow : 1. The relative small amounts of EZJ in does of a tenth of sucrose loading dose was not suppressed the elevation of blood glucose levels with oral sugar intake. 2. The relative large amounts of EZJ in does of three tenth of sucrose loading dose was significantly suppressed the elevation of blood glucose levels with oral sugar intake. Presnt results suggest thae EZJ has a suppressive effect on raising blood glucose levels after oral sucrose administration. This fact would be the advantage of EZJ in an application to the therapy or prevention of dabetes mellitus and obesity.

대동맥-장골동맥 폐색증에 대한 경피경관 혈관성형술 및 스텐트삽입술의 누적개존율

최동현,이상목,고석환,홍성화,오수명,윤충,박호철,오주형 대한혈관외과학회 2004 Vascular Specialist International Vol.20 No.1

흰쥐에서의 Puromycin Aminonucleoside-유발 단백뇨에 대한 선택적 Thromboxane A₂수용체길항제, KT2-962의 효과

서대규,신인철,고현철,하경란,강주섭 한양대학교 의과대학 1994 한양의대 학술지 Vol.14 No.1

The administration of puromycin aminonucleoside(PAN) to rats caused to nephrotic syndrome which characterized ascites, proteinurisa, hypoalbuminemia, and hyperlipidemia similar to those observed in human minimal change disease. Recently, several studies indicate that renal endogenous thromboxane(Tx) A₂may have an important role in pathophysiology of various renal disease. In this sutdy, we hafve examined the protective effct of a selective TxA₂receptor antagonist, KT2-962(KT2) on PAN-induced proteinuria in rats. Thus, male Wistar rats were given either daily subsutaneous injection of PAN, 20mg/kg, for 10 consecutive days from 3 days before to 7 days with PAN treatment. Urine was collectd, and body weight was measured in interval of 2 days during 2 weeks and urinary N-acetyl-β-Dglucosaminidase(NAG) activity as an index of renal tubular cell damage and urine protein were measured. In addition to measuring BUN, serum creatinine and creatinine clearance were measured to assess the degree of renal functional damage in 14th day. The results(Means SE) otained can be summarized as follows: 1)Body weight(gm) was progressively increased and gained about 46.4gm and 39.2gm on 2 weeks of treatment in the control and KT2 groups respectively. In constrast, there was weight loss about 27.4gm in the PAN group. But, it was increased about 23.2 gm in KT2+PAN grou and means that KT2 has significantly(p<0.05) suppressed weight loss by PAN. 2)Urine flow (ml/24 hours) was slightly increased in both control and KT2 groups during 2 weeks. But, it was significantly(p<0.05) increased after 7th groups during 2 weeks. But, it was significantly(p<0.05) increased after 7th day. But, concurrent administration of KT2 significantly(P<0.05) suppressed PAN-induced polyuria in KT2+PAN group. 3)Urinary protein(mg/24 hours0 was slightly increased in both control and KT2 groups. But, it was progressively increased and reached at the maximal level, 3.2 folds of initial level to 11th day and thereafter slightly reduced proteinuria to 14th day in the PAN group. In contrast, KT2 cotreatment with PAN was significantly(P<0.05) suppressed PAN-induced proteinuria in the KT2+PAN group. 4)Urinary NAG activity was markedly increased and reached to maximal level, 122.03 18.53 U/mg of urine creatinine, 12.7 folds of initial by day 9 and thenafter progressively decreased to 5.4 folds of initial level by day 14 in the PAN group. But, when KT2 was administered with PAN, it was significantly depressed its increment to day 13. But, it was reached to maximal level, 99.05 42.55, 12.7 folds of inital level much than PAN group. This result indicated that KT2 had a partial preventive effect on PAn-induced renal tubular cell damage. 5)The BUN and serum creatinine level(mg/dl) were significantly(p<0.05) increased from initial level, 18.48 1.28 and 0.50 0.03 to 118.42 41.34 and 1.66 0.27 respectively, and creatinine clearance(ml/min) was significantly(P<0.05) decreased from initial level, 0.44 0.02 to 0.28 0.07 by day 14 by PAN treatment. But, when PAN was given together with KT2, the increment of BUN and serum creatinine except for creatinine clearance were significantly(P<0.05) inhibited in the KT2+PAN group. Based on all these results obtained in this study, it is concluded that the coadministration of KT2-962 with PAN can be ingibited protein excretion in urine and suggested that endogenous TxA₂would take part in PAN-induced proteinuria in rats.

Factors increasing the risk for psychosocial stress among Korean adults living in rural areas

Ju-Hyun Nam,Myeong-Seob Lim,Hyun-Kyeong Choi,Jae-Yeop Kim,Sung-Kyeong Kim,Sung-Soo Oh,Sang-Baek Koh,Hee-Tae Kang 대한직업환경의학회 2017 대한직업환경의학회지 Vol.29 No.-

Background: This study was conducted to analyze the distribution of the psychosocial well-being index among adults living in two rural communities in Korea and to examine its correlation with lifestyle variables such as sleep duration, regular exercise, and sedentary time. Methods: Using the cohort data of the Atherosclerosis Risk of a Rural Area Korean General Population, this study examined 3631 participants living in Wonju and Pyeongchang in Gangwon Province; their preliminary data were established from 2005 to 2007 while their follow-up data were collected 3 years later. This study investigated demographic characteristics, lifestyle habits, disease history, Psychosocial Well-being Index-Short Form (PWI-SF) scores, sleep duration, regular exercise, and sedentary time during work. Using repeated measures ANOVA, this study examined how the variables and PWI-SF scores changed over the course of 3 years and identified the correlation between them based on mixed model analysis. Afterwards, using the generalized estimation equation, this study identified each variable’s risk towards the PWI-SF high-risk group and performed a stratified analysis by occupation after dividing the participants into farmers and non-farmers. Results: The PWI-SF high-risk group was found to be 18.9% of the participants from preliminary data and 15.5% from follow-up data. The odds ratio towards the PWI-SF high-risk group was 1.503 (95% CI 1.241–1.821) in the short sleep duration group and 1.327 (95% CI 1.136–1.550) in the non-regular exercise group. A stratified analysis by occupation showed that middle and long sedentary time in the white-collar group increased the risk toward the PWI-SF high-risk group. Conclusions: Short sleep duration, no regular exercise, and long sedentary time in the white-collar group were identified as risk factors toward the PWI-SF high-risk group in the rural communities, and policy interventions are needed to address this issue.

Secondary Sphere Formation Enhances the Functionality of Cardiac Progenitor Cells

Cho, Hyun-Jai,Lee, Ho-Jae,Youn, Seock-Won,Koh, Seok-Jin,Won, Joo-Yun,Chung, Yeon-Ju,Cho, Hyun-Ju,Yoon, Chang-Hwan,Lee, Sae-Won,Lee, Eun Ju,Kwon, Yoo-Wook,Lee, Hae-Young,Lee, Sang Hun,Ho, Won-Kyung,Par Elsevier 2012 MOLECULAR THERAPY Vol.20 No.9

난치진균각막궤양에서 냉동치료 1예

김현주(Hyun Ju Kim),임화랑(Hwa Rang Lim),고재웅(Jae Woong Koh) 대한안과학회 2017 대한안과학회지 Vol.58 No.10

목적: 항진균제에 반응하지 않는 난치진균각막궤양에서 냉동치료를 이용하여 치료한 1예를 보고하고자 한다. 증례요약: 58세 남자 환자가 2주 전부터 발생한 좌안의 통증 및 시력 저하로 좌안 각막궤양으로 진단 후 치료 받았으나 증세 악화 및 진균 감염이 의심되어 전원되었다. 내원 당시, 좌안 각막 5시 방향으로 각막혼탁 및 진행형 궤양성 병변이 관찰되었으며, 나안시력 0.025이며, 교정되지 않았다. 병변 부위에 대해 각막궤양 죽은 조직 제거술 및 균 배양, potassium hydroxide preparation (KOH) 검사를 시행하였다. 배양 검사상 Candida albicans 균 성장이 보고되었고, 점안 항생제(Fortified tobramycin, Fortified cefazolin, moxifloxacin), 점안 항진균제(Fortified amphotericin B 0.5%, Natamycin)를 투여하였으나 2주간의 투여에도 호전을 보이지 않았고, 입원 14일째 병변 부위의 냉동 치료를 시행하였다. 수술 이후 안약은 동일하게 점안하였고, 수술 이후 3일간 통증 이외의 다른 불편감은 호소하지 않았으며 각막의 병변 부위는 호전되었으며, 나안 시력도 0.32로 호전되어 수술 10일 후 퇴원하였다. 결론: 점안 항진균제에 반응하지 않는 진균성 각막궤양에 대해 냉동치료를 시행하여 시력개선 및 증상 호전을 보인 증례를 경험하였기에 이를 보고하고자 한다. Purpose: We report a case of cryotherapy for fungal corneal ulcers that did not respond to antifungal agents. Case summary: A 58-year-old man was transferred to our hospital with a left eye corneal ulcer due to pain and visual impairment in his left eye for two weeks, and he was suspected to have a history of fungal infection. At the time of admission, corneal opacity and progressive ulcerative lesions were observed at 5 o’clock in the left eye and visual acuity was 0.025, uncorrected. The corneal ulcer marginal resection, bacterial culture, and potassium hydroxide preparation (KOH) test were performed on lesion sites. Cultures of Candida albicans were reported to grow, topical antibiotics (Fortified tobramycin, Fortified cefazolin, moxifloxacin), and anti-fungal agents (fortified amphotericin B, 0.5%, Natamycin) were administered, but no improvement was observed for 2 weeks. On the 14th day after admission, Cryotherapy was performed. After surgery, eye drops were equally applied, and there was no other discomfort other than pain for 3 days after the operation. He discharged 10 days after surgery, the corneal lesion was healed and the visual acuity was improved to 0.32, uncorrected. Conclusions: We report a case of cryotherapy for fungal corneal ulcers that did not react with topical antifungal drugs and improved visual acuity and symptom improvement.

Cellular direct conversion by cell penetrable OCT4-30Kc19 protein and BMP4 growth factor

Seung Hyun L. Kim,Sungwoo Cho,Seoyeon Kim,권정후,Jaeyoung Lee,Rachel H. Koh,Ju Hyun Park,이화진,Tai Hyun Park,Nathaniel S. Hwang 한국생체재료학회 2022 생체재료학회지 Vol.26 No.3

Background: The number of patients suffering from osteoporosis is increasing as the elderly population increases. The demand for investigating bone regeneration strategies naturally arises. One of the approaches to induce bone regeneration is somatic cell transdifferentiation. Among the transcriptional regulators for transdifferentiation, octamer-binding transcription factor 4 (OCT4) is famous for its role in the regulation of pluripotency of stem cells. Bone morphogenetic protein 4 (BMP4) is another factor that is known to have a significant role in osteogenic differentiation. Previous studies have achieved transdifferentiation of cells into osteoblasts using viral and plasmid deliveries of these factors. Although these methods are efficient, viral and plasmid transfection have safety issues such as permanent gene incorporations and bacterial DNA insertions. Herein, we developed a cell penetrating protein-based strategy to induce transdifferentiation of endothelial cells into osteoblasts via nuclear delivery of OCT4 recombinant protein combined with the BMP4 treatment. For the nuclear delivery of OCT4 protein, we fused the protein with 30Kc19, a cell-penetrating and protein stabilizing protein derived from a silkworm hemolymph of Bombyx mori with low cytotoxic properties. This study proposes a promising cell-based therapy without any safety issues that existing transdifferentiation approaches had. Methods: OCT4-30Kc19 protein with high penetrating activities and stability was synthesized for a protein-based osteogenic transdifferentiation system. Cells were treated with OCT4-30Kc19 and BMP4 to evaluate their cellular penetrating activity, cytotoxicity, osteogenic and angiogenic potentials in vitro. The osteogenic potential of 3D cell spheroids was also analyzed. In addition, in vivo cell delivery into subcutaneous tissue and cranial defect model was performed. Results: OCT4-30Kc19 protein was produced in a soluble and stable form. OCT4-30Kc19 efficiently penetrated cells and were localized in intracellular compartments and the nucleus. Cells delivered with OCT4-30Kc19 protein combined with BMP4 showed increased osteogenesis, both in 2D and 3D culture, and showed increased angiogenesis capacity in vitro. Results from in vivo subcutaneous tissue delivery of cell-seeded scaffolds confirmed enhanced osteogenic properties of transdifferentiated HUVECs via treatment with both OCT4-30Kc19 and BMP4. In addition, in vivo mouse cranial defect experiment demonstrated successful bone regeneration of HUVECs pretreated with both OCT4-30Kc19 and BMP4. Conclusions: Using a protein-based transdifferentiation method allows an alternative approach without utilizing any genetic modification strategies, thus providing a possibility for safer use of cell-based therapies in clinical applications.

DNA Ligase4 as a Prognostic Marker in Nasopharyngeal Cancer Patients Treated with Radiotherapy

Kim, Dong Hyun,Oh, Sung Yong,Kim, So Yeon,Lee, Seul,Koh, Myeong Seok,Lee, Ji Hyun,Lee, Suee,Kim, Sung-Hyun,Park, Heon Soo,Hur, Won Joo,Jeong, Jin Sook,Ju, Mi Ha,Seol, Young Mi,Choi, Young-Jin,Chung, J Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

Background: The capability for DNA double-strand breaks (DSBs) repair is crucial for inherent radiosensitivity of tumor and normal cells. We have investigated the clinicopathologic significance of DNA repair gene expression in nasopharyngeal (NP) carcinoma. Materials and Methods: A total of 65 NP cancer patients who received radiotherapy were included. The immunopositivity to Ku 70, DNA-PKcs, MRN, RAD50, XRCC4, and LIG4 were examined in all tumor tissues. Results: The patients comprised 42 males and 23 females, with a median age of 56 years (range, 18-84). The expression levels of RAD50 (0,+1,+2,+3) were 27.7%, 32.3%, 21.5%, and 18.5%. LIG4 (${\pm}$) were 43.1% and 56.9% respectively. The 5-year OS rate of patients with LIG4 (${\pm}$) were 90% and 67.9%, respectively (p=0.035). The 5-year TTP rate of patients with LIG4 (${\pm}$) were 75.9%, 55.5%, respectively (P=0.039). Conclusions: Our results suggest the possibility of predicting the radiosensitivity of NP cancer by performing immunohistochemical analysis of LIG4.