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Cellulase생산을 위한 Verticillium sp.의 배양조건의 검토
홍대희,진효상,오두환,유주현 연세대학교 산업기술연구소 1986 논문집 Vol.18 No.1
The cultivation conditions on wheat bran of verticillium sp. were optimized to produce cellulase as much as possible. The highest cellulase activity was shown, as verticillium sp. was cultivated at 27℃ for 5 days on wheat bran media with 1.2㎖/g wheat bran of water content and supplementation of NaNo and FeSo, in concentration of 0.1% and 0.2-0.3%, respectively.
Verticillium sp.가 생산하는 cellulase의 정제 및 그 성질
유주현,홍대희,양융,진효상 연세대학교 산업기술연구소 1986 논문집 Vol.18 No.1
The cellulase of verticillium sp. was purified to investigate its some properties. The enzyme purification was carried out by ammonium sulfate fractionation, and column chromatography of Sephadex G-50, G-100 and DEAE Sephadex A-25, with its specific activity increased to 17 fold. The molecular weight was estimated to be 46,000 by gel filtration. The optimum pH and temperature were pH 7 and 50℃, respectively. The enzyme was stable over the range of pH5-7 at 50℃ for 1 hour. But half of its activity was lost after 2 hour. The km value was calculated to be 1.2mg of CMC/㎖.
정재홍,심창주,신영,권효진,이규희,오만진 한국식품영양학회 1999 韓國食品營養學會誌 Vol.12 No.5
인삼이 밀가루의 아밀로그래프에 의한 호화 성질과 파리노그래프에 의한 반죽 성질에 미치는 영향을 조사하였다. 인삼첨가 LL면의 조직감과 관능적 특성에 미치는 영향을 분석하기 위하여 밀가루에 대하여 인삼을 5.0∼10% 첨가하여 LL면을 제조한 뒤 면의 조직 특성 분석 및 조리시험을 하고 관능검사를 하였다. 인삼을 첨가하면 아밀로그래프의 호화개시 온도를 3.1℃ 지연시켰으며, 최고 점도를 40B.U 감소시켰다. 파리노그래프의 흡수율은 1.2% 증가하였으며, 반죽의 안정도는 인삼의 첨가로 2.0분 길게 측정되었다. Hunter L값은 인삼 첨가량이 증가할수록 낮아졌으며, Hunter a, b값은 반대로 측정되었다. 인삼첨가 LL면의 층밀림 압출력은 첨가량의 증가에 따라 높아져 10.0%의 첨가는 대조구 18.65(㎏f)보다 2.3(㎏f) 높은 23.95(㎏f)로 측정되었으며, 경도도 대조구보다 5.4(㎏f) 높게 측정되었다. 인삼첨가 LL면의 무게 증가는 인삼의 첨가량이 증가할 때 다소 높게 나타났으나. 부피 증가는 오히려 높게 나타나 조직이 다소 치밀한 것으로 나타났다 용출량은 인삼량의 증가에 따라 약간 감소하였다. 미생물은 인삼의 첨가량이 증가할수록 줄어들어서 장기 저장이 가능하였다. 관능 검사 결과는 인삼첨가 LL면이 4.2 및 4.0으로 비교적 좋은 점수를 얻었다. 이같이 LL면 제조시 인삼 첨가량은 7.0%의 수준이 효과가 컸다. The influence of ginseng on the paste or gelatinization properties by amylograph and mixing properties by farinograph of wheat flour, and on quality properties, color, cooking quality, textural and sensory properties, and reducing microbial population of LL (Long Life) noodles was studied. The contents of ginseng used were from 5% to 10% based on flour weight. The viscosity property of wheat flour with ginseng was increased the initial pasting temperature but the amylograph peak viscosity were decreased in vice versa. The farinograph absorption, stability and breakdown were increased by ginseng. The whiteness of LL noodles manufactured with ginseng was lower than that of control. The shear extrusion force and hardness of LL noodles manufactured with ginseng were shown much higher value than those of control. At cooking quality examination of LL noodles manufactured with ginseng, weight of cooked LL noodles was decreased but volume was appeared in vice versa. Extraction amounts of LL noodles manufactured with ginseng during cooking were much smaller than those of control. Total counts of microorganism of LL noodles manufactured with ginseng were decreased during storage at 30℃. Sensory properties of cooked LL noodles which was manufactured with ginseng showed quite acceptable. Based on the cooking and sensory evaluation test, addition of 7.0% ginseng to wheat flour may be suitable for processing LL noodles.
Hong, Ji Hyun,Lee, Hyo Chun,Choi, Kyu Hye,Moon, Seok Whan,Kim, Kyung Soo,Hong, Suk Hee,Hong, Ju-Young,Kim, Yeon-Sil,Multidisciplinary Team of Lung Cancer in Seoul St. Mary's Hospital The Korean Society for Radiation Oncology 2019 Radiation Oncology Journal Vol.37 No.2
Purpose: The purpose of this study is to evaluate the safety and efficacy of the multimodality treatment with neoadjuvant intensity-modulated radiotherapy (IMRT) for resectable clinical T1-3N0-1M0 malignant pleural mesothelioma (MPM). Materials and Methods: A total of eleven patients who received neoadjuvant chemotherapy and radiotherapy between March 2016 and June 2018 were reviewed. Patients received 25 Gy in 5 fractions to entire ipsilateral hemithorax with helical tomotherapy. Results: All of patients were men with a median age of 56 years. Epithelioid subtype was found in 10 patients. All patients received neoadjuvant chemotherapy with pemetrexed-cisplatin regimen. Ten patients (90.9%) completed 25 Gy/5 fractions and one (9.0%) completed 20 Gy/4 fractions of radiotherapy. IMRT was well tolerated with only one acute grade 3 radiation pneumonitis. Surgery was performed 1 week (median, 8 days; range, 1 to 15 days) after completing IMRT. Extrapleural pneumonectomy was performed in 4 patients (36.3%), extended pleurectomy/decortication in 2 (18.2%) and pleurectomy/decortications in 5 (63.6%). There was no grade 3+ surgical complication except two deaths after EPP in 1 month. Based on operative findings and pathologic staging, adjuvant chemotherapy was delivered in 7 patients (63.6%), and 2 (18.2%) were decided to add adjuvant radiotherapy. After a median follow-up of 14.6 months (range, 2.8 to 30 months), there were 3 local recurrence (33.3%) and 1 distant metastasis (11.1%). Conclusion: Neoadjuvant entire pleural IMRT can be delivered with a favorable radiation complication. An optimal strategy has to be made in resectable MPM patients who would benefit from neoadjuvant radiation and surgery. Further studies are needed to look at long-term outcomes.
Ji Hyun Hong,Hyo Chun Lee,Kyu Hye Choi,Seok Whan Moon,Kyung Soo Kim,Suk Hee Hong,Ju-Young Hong,Yeon-Sil Kim,for Multidisciplinary Team of Lung Cancer in Seoul St,Mary’s Hospital 대한방사선종양학회 2019 Radiation Oncology Journal Vol.37 No.2
Purpose: The purpose of this study is to evaluate the safety and efficacy of the multimodality treatment with neoadjuvant intensitymodulated radiotherapy (IMRT) for resectable clinical T1-3N0-1M0 malignant pleural mesothelioma (MPM). Materials and Methods: A total of eleven patients who received neoadjuvant chemotherapy and radiotherapy between March 2016 and June 2018 were reviewed. Patients received 25 Gy in 5 fractions to entire ipsilateral hemithorax with helical tomotherapy. Results: All of patients were men with a median age of 56 years. Epithelioid subtype was found in 10 patients. All patients received neoadjuvant chemotherapy with pemetrexed-cisplatin regimen. Ten patients (90.9%) completed 25 Gy/5 fractions and one (9.0%) completed 20 Gy/4 fractions of radiotherapy. IMRT was well tolerated with only one acute grade 3 radiation pneumonitis. Surgery was performed 1 week (median, 8 days; range, 1 to 15 days) after completing IMRT. Extrapleural pneumonectomy was performed in 4 patients (36.3%), extended pleurectomy/decortication in 2 (18.2%) and pleurectomy/decortications in 5 (63.6%). There was no grade 3+ surgical complication except two deaths after EPP in 1 month. Based on operative findings and pathologic staging, adjuvant chemotherapy was delivered in 7 patients (63.6%), and 2 (18.2%) were decided to add adjuvant radiotherapy. After a median follow-up of 14.6 months (range, 2.8 to 30 months), there were 3 local recurrence (33.3%) and 1 distant metastasis (11.1%). Conclusion: Neoadjuvant entire pleural IMRT can be delivered with a favorable radiation complication. An optimal strategy has to be made in resectable MPM patients who would benefit from neoadjuvant radiation and surgery. Further studies are needed to look at long-term outcomes.
김효주 ( Kim Hyo-ju ),이선주 ( Lee Sun-ju ),계홍찬 ( Gye Hong-chan ),이승중 ( Lee Seung-joong ),조창근 ( Cho Chang-geun ) 한국구조물진단유지관리공학회 2023 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.27 No.1
최근에 발생하는 지진에 의해 우리나라 대지진 발생 위험성이 증가하고 있다. 최근까지도 규모 2.0에서 3.7의 지진이 여러 지역에서 발생하였다. 이러한 근거로 우리나라의 대지진 발생 위험을 배제할 수 없다. 지진에 대비하기 위해 강도증진공법, 연성능력 향상법, 에너지 소산능력 향상을 통한 제진 및 면진 공법 등 많은 내진보강공법들이 개발되었다. 본 연구는 6층 미만의 중 · 저층 철근콘크리트 건축물의 전단파괴 위험성을 낮추는 강성 및 내진을 증진시키는 내진보강법 기술로서, 다양한 보강 설계 및 상세에 따른 내진 효율성을 비교 평가하였다.
Characteristics and Risk Factors of Aspiration in Lateral Medullary Infarction
Ju Sun Kim,Hyun Jung Kim,Jun Yup Kim,Hyo Seon Choi,Jun-taek Hong,김덕용 대한연하장애학회 2020 대한연하장애학회지 Vol.10 No.1
Objective: To evaluate the characteristics of dysphagia and identify the risk factors of bolus aspiration in patientspresenting with pure lateral medullary infarction (LMI). Methods: Between January 2014 and January 2019, 51 post-stroke patients with LMI who underwent a videofluoroscopicswallowing study (VFSS) were enrolled retrospectively, and their medical records and brain magnetic resonanceimaging results were reviewed. The VFSS results were evaluated to analyze the swallowing function using thepenetration-aspiration scale, functional dysphagia scale, and imaging analysis software. Results: Bolus aspiration was detected in 21 patients (41.2%). The common abnormal VFSS findings were residue invalleculae (74.5%), delayed triggering of pharyngeal swallow (72.5%), residue in pyriform sinuses (62.7%), delayed pharyngealtransit time (56.9%), reduced laryngeal elevation (51.0%), and coating of the pharyngeal wall (49.0%). Theincidence of aspiration was significantly higher in the typical lesions (including the diagonal band-shaped lesions)and the large type lesions extending ventrally or dorsally, as compared to other lesion types (P<0.05). Logistic regressionanalyses revealed that the residue in pyriform sinuses is a significant independent risk factor of aspirationin the puree trial, and prolonged pharyngeal delay time (PDT) and residue in valleculae are significant risk factorsin the thin liquid trial (P<0.05). Conclusion: Considering all clinical factors, lesion locations, and swallowing processes, results of the current studyindicate that residue in pyriform sinuses is an independent risk factor of aspiration in the swallowing puree technique,whereas prolonged PDT and residue in valleculae are independent risk factors of aspiration in the swallowingliquid technique.