Using Online Respondent Driven Sampling for Vietnamese Youths’ Alcohol Use and Associated Risk Factors

Melvyn WB Zhang,Bach Xuan Tran,Huong Lan Thi Nguyen,Huong Thi Le,Nguyen Hoang Long,Huong Thi Le,Nguyen Duc Hinh,Tran Dinh Tho,Bao Nguyen Le,Vu Thi Minh Thuc,Chau Ngo,Nguyen Huu Tu,Carl A. Latkin,Roger 대한의료정보학회 2017 Healthcare Informatics Research Vol.23 No.2

Objectives: The average alcohol consumption per capita among Vietnamese adults has consistently increased. Although alcoholrelated disorders have been extensively studied, there is a paucity of research shedding light on this issue among Internet users. The study aimed to examine the severity of alcohol-related disorders and other associated factors that might predispose individuals towards alcohol usage in a sample of youths recruited online. Methods: An online cross-sectional study was conducted with 1,080 Vietnamese youths. A standardized questionnaire was used. Respondent-driven sampling was applied to recruit participants. Multivariate logistic and Tobit regressions were utilized to identify the associated factors. Results: About 59.5% of the males and 12.7% of the total youths declared that they were actively using alcohol. From the total sample, a cumulative total of 32.3% of the participants were drinking alcohol, with 21.8% and 25.0% of the participants being classified as drinking hazardously and binge drinkers, respectively. The majority of the participants (60.7%) were in the pre-contemplative stage. Conclusions: A high prevalence of hazardous drinking was recognized among online Vietnamese youths. In addition, we found relationships between alcohol use disorder and other addictive disorders, such as tobacco smoking and water-pipe usage. Our results highlighted that the majority of the individuals are not receptive to the idea of changing their alcohol habits, and this would imply that there ought to be more government effort towards the implementation of effective alcohol control policies.

Bioactive Compounds and Chemical Biology : Biotransformation of Rosamicin Antibiotic into 10,11-Dihydrorosamicin with Enhanced In Vitro Antibacterial Activity Against MRSA

( Nguyen Lan Huong ),( Nguyen Huu Hoang ),( Anil Shrestha ),( Jae Kyung Sohng ),( Yeo Joon Yoon ),( Je Won Park ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.1

A biotransformation approach using microbes as biocatalysts can be an efficient tool for the targeted modification of existing antibiotic chemical scaffolds to create previously uncharacterized therapeutic agents. By employing a recombinant Streptomyces venezuelae strain as a microbial catalyst, a reduced macrolide, 10,11-dihydrorosamicin, was created from rosamicin macrolide. Its chemical structure was spectroscopically elucidated, and the new rosamicin analog showed 2-4-fold higher antibacterial activity against two strains of methicillin-resistant Staphylococcus aureus compared with its parent rosamicin. This kind of biocatalytic approach is able to expand existing antibiotic entities and can also provide more diverse therapeutic resources.

Data Analytics Application: A Case Study of Online Business for Vietnamese Handicraft Products on Amazon

Lan, Nguyen Thi Thao,Phuong, Nguyen Pham Anh,Trang, Nguyen Thi My,Huong, Pham Thi My,An, Nguyen Thu,Le, Hoanh-Su Korea Multimedia Society 2021 The journal of multimedia information system Vol.8 No.1

The paper is based on data collected from the Amazon website (specific in the Handmade's Category) to understand and analyze Vietnamese artisans' business context. Data analysis is also applied to determine the factors that bring success Handmade products and compare products of the same industry among competitors to find out potential products. By collecting data from Amazon and analyzing the data, we extracted useful information for online business developers. Besides, the list of potential products in Handmade sector can be referred to improve the business and compete with competitors. This paper also proposes solutions to help Vietnamese products become more appealing to international customers on the Amazon website.

Characterization of fortimicin aminoglycoside profiles produced from Micromonospora olivasterospora DSM 43868 by high-performance liquid chromatography-electrospray ionization-ion trap-mass spectrometry

Huong, Nguyen Lan,Hoang, Nguyen Huu,Hong, Sung-Yong,Sohng, Jae Kyung,Yoon, Yeo Joon,Park, Je Won Springer-Verlag 2016 Analytical and Bioanalytical Chemistry Vol.408 No.6

<P>In this study, an efficient high-performance liquid chromatography (HPLC)-electrospray ionization (ESI)-ion trap-tandem mass spectrometry (MS/MS) was developed for the identification of the biosynthetic congeners involved in the aminocyclitol aminoglycosidic fortimicin pathway from Micromonospora olivasterospora fermentation. The usage of both acid extraction (pH similar to 2.5) followed by an cationic-exchanging SPE cleanup and pentafluoropropionic acid mediated ion-pairing chromatography with ESI-ion trap-MS/MS detection was determined to be sufficiently practical to profile the fortimicin (FOR) congeners produced in a culture broth. The limit of the quantification for the fortimicin A (FOR-A) standard spiked in the culture broth was similar to 1.6 ng mL(-1). The average recovery rate was 93.6 %, and the intra- and inter-day precisions were < 5 % with accuracy in the range from 87.1 to 94.2 %. Moreover, the epimeric mixtures including FOR-KH, FOR-KR, and FOR-B were separately resolved through a macrocyclic glycopeptide (teicoplanin)-bonded chiral column. As a result, ten natural FOR pseudodisaccharide analogs were identified and semi-quantified in descending order as follows: FOR-A, FOR-B, DCM, FOR-KH plus FOR-KR, FOR-KK1, FOR-AP, FOR-KL1, FOR-AO, and FOR-FU-10. This is the first report on both the simultaneous characterization of diverse structurally closely related FORs derived from bacterial fermentation using HPLC-ESI-ion trap-MS/MS analysis and the chromatographic separation of the three FOR epimers.</P>

Financial Development and Economic Growth: Credit Distribution in Southeast Asian Countries

Lan Thi Huong NGUYEN,Anh Le Dieu NGUYEN,Huyen Thanh LE,Duy Van NGUYEN 한국유통과학회 2024 유통과학연구 Vol.22 No.3

Reaction Kinetics of Substrate Transglycosylation Catalyzed by TreX of <i>Sulfolobus solfataricus</i> and Effects on Glycogen Breakdown

Nguyen, Dang Hai Dang,Park, Jong-Tae,Shim, Jae-Hoon,Tran, Phuong Lan,Oktavina, Ershita Fitria,Nguyen, Thi Lan Huong,Lee, Sung-Jae,Park, Cheon-Seok,Li, Dan,Park, Sung-Hoon,Stapleton, David,Lee, Jin-Sil American Society for Microbiology 2014 Journal of Bacteriology Vol.196 No.11

<P>We studied the activity of a debranching enzyme (TreX) from <I>Sulfolobus solfataricus</I> on glycogen-mimic substrates, branched maltotetraosyl-β-cyclodextrin (Glc<SUB>4</SUB>-β-CD), and natural glycogen to better understand substrate transglycosylation and the effect thereof on glycogen debranching in microorganisms. The validation test of Glc<SUB>4</SUB>-β-CD as a glycogen mimic substrate showed that it followed the breakdown process of the well-known yeast and rat liver extract. TreX catalyzed both hydrolysis of α-1,6-glycosidic linkages and transglycosylation at relatively high (>0.5 mM) substrate concentrations. TreX transferred maltotetraosyl moieties from the donor substrate to acceptor molecules, resulting in the formation of two positional isomers of dimaltotetraosyl-α-1,6-β-cyclodextrin [(Glc<SUB>4</SUB>)<SUB>2</SUB>-β-CD]; these were 6<SUP>1</SUP>,6<SUP>3</SUP>- and 6<SUP>1</SUP>,6<SUP>4</SUP>-dimaltotetraosyl-α-1,6-β-CD. Use of a modified Michaelis-Menten equation to study substrate transglycosylation revealed that the <I>k</I><SUB>cat</SUB> and <I>K<SUB>m</SUB></I> values for transglycosylation were 1.78 × 10<SUP>3</SUP> s<SUP>−1</SUP> and 3.30 mM, respectively, whereas the values for hydrolysis were 2.57 × 10<SUP>3</SUP> s<SUP>−1</SUP> and 0.206 mM, respectively. Also, enzyme catalytic efficiency (the <I>k</I><SUB>cat</SUB>/<I>K<SUB>m</SUB></I> ratio) increased as the degree of polymerization of branch chains rose. In the model reaction system of <I>Escherichia coli</I>, glucose-1-phosphate production from glycogen by the glycogen phosphorylase was elevated ∼1.45-fold in the presence of TreX compared to that produced in the absence of TreX. The results suggest that outward shifting of glycogen branch chains via transglycosylation increases the number of exposed chains susceptible to phosphorylase action. We developed a model of the glycogen breakdown process featuring both hydrolysis and transglycosylation catalyzed by the debranching enzyme.</P>

Establishment of a NanoBiT-Based Cytosolic Ca2+ Sensor by Optimizing Calmodulin-Binding Motif and Protein Expression Levels

Lan Phuong Nguyen,Huong Thi Nguyen,용효정,Arfaxad Reyes-Alcaraz,Yoo-Na Lee,Hee-Kyung Park,Yun Hee Na,이철순,Byung-Joo Ham,Jae Young Seong,황종익 한국분자세포생물학회 2020 Molecules and cells Vol.43 No.11

Cytosolic Ca2+ levels ([Ca2+]c) change dynamically in response to inducers, repressors, and physiological conditions, and aberrant [Ca2+]c concentration regulation is associated with cancer, heart failure, and diabetes. Therefore, [Ca2+]c is considered as a good indicator of physiological and pathological cellular responses, and is a crucial biomarker for drug discovery. A genetically encoded calcium indicator (GECI) was recently developed to measure [Ca2+]c in single cells and animal models. GECI have some advantages over chemically synthesized indicators, although they also have some drawbacks such as poor signal-to-noise ratio (SNR), low positive signal, delayed response, artifactual responses due to protein overexpression, and expensive detection equipment. Here, we developed an indicator based on interactions between Ca2+-loaded calmodulin and target proteins, and generated an innovative GECI sensor using split nano-luciferase (Nluc) fragments to detect changes in [Ca2+]c. Stimulation-dependent luciferase activities were optimized by combining large and small subunits of Nluc binary technology (NanoBiT, LgBiT:SmBiT) fusion proteins and regulating the receptor expression levels. We constructed the binary [Ca2+]c sensors using a multicistronic expression system in a single vector linked via the internal ribosome entry site (IRES), and examined the detection efficiencies. Promoter optimization studies indicated that promoter-dependent protein expression levels were crucial to optimize SNR and sensitivity. This novel [Ca2+]c assay has high SNR and sensitivity, is easy to use, suitable for high-throughput assays, and may be useful to detect [Ca2+]c in single cells and animal models.

Environmental-friendly method for preparing CoFe2O4 coated biopolymer extracted from dragon fruit peel: Characterization and application as nanocomposite adsorbent for removal of As(III) pollutants from aqueous solution

Lan Huong Nguyen,Van Son Le,Luu Dung Tran,Nam Van Thai,Ho Thi Ngoc Tram,Bui Quang Minh,Van Huy Nguyen 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.118 No.-

This study successfully developed a low-cost adsorbent from compositing between magnetic nanoparticle(CoFe2O4) and dragon fruit peel-derived biopolymer (DFP-BP) and applied it to remove arsenite (As(III)) from contaminated water. The batch experiments were designed to study the influence of operationalparameters on As(III) adsorption by nanocomposite (CoFe2O4@DFP-BP). With mapping analysis,the synthesized CoFe2O4@DFP-BP was characterized using SBET, SEM, FTIR, XRD, and EDS mapping. TheAs(III) adsorption mechanism was discussed based on material property data and isotherm and kineticanalysis. The result suggests that 5% is the best modification ratio on the CoFe2O4@DFP-BP for As(III)adsorption. The highest adsorption capacity of As(III) under the optimal conditions of pH 7, adsorbentdosage of 1.6 g/L, initial As(III) concentration of 2000 lg/L and the best described by the Sips modelwas 1922.7 lg/g. The adsorption kinetic followed pseudo-second-order, proving As(III) adsorption processcontrolled by chemisorption. The primary reaction pathway of As(III) adsorption on theCoFe2O4@DFP-BP5 was inner-sphere complexation through exchange between the nanoadsorbent’s surfaceand As(III) ions via oxygen-containing functional (carboxyl and hydroxyl) groups. The CoFe2O4 magneticnanoparticles coated by biopolymer overcame drawbacks, including low stability and mechanicalstrength of biopolymer and agglomerate trend of magnetic nanoparticles. The adsorption process washighly reversible and accessible in the separation of nanoadsorbent after adsorption by the magnet. Therefore, the nanocomposite formed from solid waste has excellent potential as a material for removingAs, contributing to sustainable development and feasibility in practical application.

Detection of porcine reproductive and respiratory syndrome virus in oral fluid from naturally infected pigs in a breeding herd

Nguyen Thi Trang,Takuya Hirai,Tsukasa Yamamoto,Mari Matsuda,Naoko Okumura,Nguyen Thi Huong Giang,Nguyen Thi Lan,Ryoji Yamaguchi 대한수의학회 2014 Journal of Veterinary Science Vol.15 No.3

The objectives of the present study were to evaluate theanatomic localization of porcine reproductive andrespiratory syndrome virus (PRRSV) in naturally infectedpigs and to determine whether oral fluid could be used todetect the virus in infected animals. Two sows, seven2-month-old grower pigs, and 70 6-month-old gilts wereincluded in this study. PRRSV in sera and oral fluid wereidentified by nested reverse transcription PCR (nRT-PCR)while lung, tonsil, and tissue associated with oral cavity weresubjected to nRT-PCR, immunohistochemistry, and in situhybridization. In sows, PRRSV was identified in oral fluidand tonsils. PRRSV was also detected in oral fluid, tonsils,salivary glands, oral mucosa, and lungs of all seven growerpigs. However, viremia was observed in only two growerpigs. Double staining revealed that PRRSV was distributedin macrophages within and adjacent to the tonsillar cryptepithelium. In gilts, the North American type PRRSV fieldstrain was detected 3 to 8 weeks after introducing theseanimals onto the farm. These results confirm previousfindings that PRRSV primarily replicates in tonsils and isthen shed into oral fluid. Therefore, oral fluid sampling maybe effective for the surveillance of PRRSV in breeding herds.

Preparation and Adhesive Properties of Complex Gels from Polyaspartamides and Tannic Acid

Huong Lan Nguyen Ngoc(훵란뉜녹),Jong Ryoul Moon(문종렬),Ji-Heung Kim(김지흥) 한국고분자학회 2019 폴리머 Vol.43 No.5

몇 가지 다른 폴리아스팔트아미드 유도체를 아스팔트산의 열축합 고분자인 폴리숙신이미드의 에탄올아민과 또 다른 아민 화합물을 사용한 연속적인 개환 아미노분해 반응으로부터 제조하였다. 합성한 폴리아스팔트아미드 고분자의 화학적 구조를 NMR과 FTIR 분광기를 사용하여 확인하였다. 물리적 가교제 및 접착물성 증진제로서 탄닌산과 위에서 합성한 고분자를 수용액 상에서 여러 다른 중량비로 혼합함으로써 두 성분간의 컴플렉스 젤을 제조하였다. 이들 컴플렉스 젤 형성 거동과 제조된 젤 시료의 서로 다른 기질과 조건에 대한 접착 물성을 비교 검토하였다. 한편, 열중량분석과 FTIR을 이용하여 재료의 열분해 특성과 두 성분간의 분자간 상호작용을 고찰하였다. 성분 간 강한 수소결합에 기인한 이들 생체적합성 폴리아미노산 고분자와 탄닌산의 컴플렉스 젤은 신규 생체의료용 및 산업용 접착소재로서 응용 가능할 것으로 기대된다. Several polyaspartamide copolymers were prepared from polysuccinimide, the thermal polycondensation product of aspartic acid, via a successive ring-opening reaction using ethanolamine and other amine nucleophiles. 1H NMR and FTIR spectroscopy were used to identify the chemical structure of the polyaspartamide copolymers. A complex gel was formed upon adding tannic acid (TA) as both a physical cross-linker and adhesive enhancer to the polymer solution at different compositions in an aqueous system. The complexation behavior was investigated and the adhesive properties of the resulting complex gels were measured and compared each other. The thermal properties of the complex gels were also investigated using thermogravimetric analysis. Strong multiple hydrogen-bonding interactions between TA and the polymer chain are considered to be responsible for the formation of these biocompatible complex gels, which have potential use as novel biocompatible adhesive materials.