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Ham, Heejin,Jang, Jungim,Jo, Sukju,Oh, Younghee,Pak, Sonil 대한미생물학회 2014 Journal of Bacteriology and Virology Vol.44 No.3
For our survey of the infection frequency and mixed infection of the viruses causing acute respiratory syndromes, we analyzed those viruses from acute respiratory patients in Seoul. Total 1,038 specimens of oropharyngeal swab were tested by the real-time polymerase chain reaction (PCR) kit (Kogenebiotech, Korea) from Jan. to Dec. in 2013. Virus detection rate causing acute respiratory infection was 46% (476/1,038). The most frequently isolated virus was only hRV (21.6%, 103/476), followed by only ADV (8.96%, 93/476), only IFV A (H3N2) (18.1%, 86/476), and only hCoV (7.8%, 37/476) etc. Most of acute respiratory viruses had severe fever. Infection frequency information and mixed infection status on respiratory viruses circulating in Seoul will be helpful for the management of acute respiratory infection and for epidemiological continuous studies.
Ham, Heejin,Oh, Seah,Jo, Sukju,Choi, Sungmin 대한미생물학회 2014 Journal of Bacteriology and Virology Vol.44 No.1
Antigen distributions of rotavirus and adenovirus were detected by enzyme immuno assay (EIA) in patients with acute gastroenteritis in Seoul from 2008 to 2012. Tested feces sample numbers from patients were 9,597. Antigen distributions of rotavirus and adenovirus were 13.9%, and 2.1%, respectively. The patterns of each viral pathogen may give potentially effective data for epidemiological studies.
Heejin Ham,Sukju Jo,Jungim Jang,Sungmin Choi 대한기생충학열대의학회 2014 The Korean Journal of Parasitology Vol.52 No.2
Larvae, nymphs, and adult stages of 3 species of ixodid ticks were collected by tick drag methods in Seoul during June-October 2013, and their infection status with severe fever with thrombocytopenia syndrome (SFTS) virus was examined using RT-PCR. During the period, 732 Haemaphysalis longicornis, 62 Haemaphysalis flava, and 2 Ixodes nipponensis specimens were collected. Among the specimens of H. longicornis, the number of female adults, male adults, nymphs, and larvae were 53, 11, 240, and 446, respectively. Ticks were grouped into 63 pools according to the collection site, species, and developmental stage, and assayed for SFTS virus. None of the pools of ticks were found to be positive for SFTS virus gene.
( Heejin Ham ),( Ga-hyun Son ),( Keun-young Lee ),( Ji Eun Song ) 대한산부인과학회 2018 대한산부인과학회 학술대회 Vol.104 No.-
Objective: The purpose of this study was to compare the pregnancy outcomes of history-indicated cerclage (HIC) and transabdominal cerclage (TAC) in twin pregnancy. Methods: Retrospective chart review of twin pregnancies with HIC or TAC was performed. The study population underwent cerclage at Hallym University Kangnam Sacred Heart Hospital from 2007 to 2018. We compared the pregnancy outcomes between HIC and TAC group; The cerclage-to-delivery interval, the mean gestational age (GA) at cerclage, the mean GA at delivery, and birth weight were analyzed; The incidence of preterm birth (PTB) less than 28 weeks and 34 weeks, neonatal mortality were also compared between two groups. Results: Fifty-two patients were included; 19 patients underwent HIC, and 32 underwent TAC. There was no difference in the mean GA at cerclage between two groups (HIC, 13.73 weeks vs. TAC, 13.83 weeks, p=0.574). The mean GA at delivery in HIC group was 31.77 weeks, and that in TAC group was 35.85 weeks (p=0.003). There was statistically significant difference in the mean cerclage-to-delivery interval between two groups (HIC, 131.21days vs. TAC, 154days, p= 0.032) There were significant difference in neonatal birthweight between two groups (1st baby, 1846.84g vs. 2417.27g (p=0.004); 2nd baby 1732.11g vs. 2353.33, (p=0.001)), and neonatal mortality (HIC 4/19(21.1%) vs. TAC 1/33(3.0%), p=0.054). TAC group showed lower incidence of PTB < 28 weeks compared to HIC group (HIC 4/19 (21.1%) vs. TAC 1/33(3.0%), p=0.054). There was statistical difference in the incidence of PTB < 34weeks (HIC group, 8/19(42.1 %) vs. TAC group, 2/33(6.1 %), p=0.003). Conclusion: Both HIC and TAC were beneficial in prolonging pregnancy in twin pregnancy. TAC in twin pregnancy showed more favorable obstetric outcomes when compared to HIC.
Decision between mitophagy and apoptosis by Parkin via VDAC1 ubiquitination
Ham, Su Jin,Lee, Daewon,Yoo, Heesuk,Jun, Kyoungho,Shin, Heejin,Chung, Jongkyeong National Academy of Sciences 2020 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.117 No.8
<P><B>Significance</B></P><P>VDAC1 transports ions and small molecules at the mitochondrial outer membrane. In this study, we discover that Parkin, a frequently mutated Parkinson disease protein, ubiquitinates VDAC1 in two different manners, poly- and monoubiquitination. Interestingly, VDAC1 defective in polyubiquitination hinders Parkin-mediated mitophagy, but VDAC1 defective in monoubiquitination induces apoptosis. When VDAC1 deficient with monoubiquitination is expressed in mammalian cells and fruit fly, we observe increased mitochondrial calcium influx and apoptosis and typical in vivo phenotypes related to Parkinson disease, such as defective locomotive activity and loss of dopaminergic neurons. These results suggest that the dual regulation of mitophagy and apoptosis by Parkin via VDAC1 poly- and monoubiquitination is highly critical in protecting cells from the pathogenesis of Parkinson disease.</P><P>VDAC1 is a critical substrate of Parkin responsible for the regulation of mitophagy and apoptosis. Here, we demonstrate that VDAC1 can be either mono- or polyubiquitinated by Parkin in a PINK1-dependent manner. VDAC1 deficient with polyubiquitination (VDAC1 Poly-KR) hampers mitophagy, but VDAC1 deficient with monoubiquitination (VDAC1 K274R) promotes apoptosis by augmenting the mitochondrial calcium uptake through the mitochondrial calcium uniporter (MCU) channel. The transgenic flies expressing <I>Drosophila</I> Porin K273R, corresponding to human VDAC1 K274R, show Parkinson disease (PD)-related phenotypes including locomotive dysfunction and degenerated dopaminergic neurons, which are relieved by suppressing MCU and mitochondrial calcium uptake. To further confirm the relevance of our findings in PD, we identify a missense mutation of Parkin discovered in PD patients, T415N, which lacks the ability to induce VDAC1 monoubiquitination but still maintains polyubiquitination. Interestingly, <I>Drosophila</I> Parkin T433N, corresponding to human Parkin T415N, fails to rescue the PD-related phenotypes of <I>Parkin</I>-null flies. Taken together, our results suggest that VDAC1 monoubiquitination plays important roles in the pathologies of PD by controlling apoptosis.</P>