H-Y 에 대한 단일클론 항체의 생산 및 그 이용에 관한 연구 1 . H-Y 에 대한 단일클론항체의 생산

심호섭(H . S . Shim),김재화(J . H . Kim),이병철(B . C . Lee),김종배(J . B . Kim),박홍양(H . Y . Park),정길생(K . S . Chung) 한국축산학회 1988 한국축산학회지 Vol.30 No.7

Testis supernatant, a source of H-Y, obtained from BALB/c mice was used to immunize females of same strain. B lymphocytes of mouse producing antibodies to H-Y were fused with SP2/0-Ag 14 myeloma cells and distributed to 384 wells of 96-well microtiter plates. Eighty hybridoma colonies were formed, resulting in 20.8 percent of fusion efficiency. Three strong positive wells from hybridoma colonies were selected for cloning by ELISA and two of them were also found to be positive by indirect immunofluorescence test. Twelve wells of ELISA-positive were selected after cloning and 2D45D4 clones from them were confirmed to produce monoclonal antibodies to H-Y by indirect immunofluorescence test.

VP2 capsid domain of the H-1 parvovirus determines susceptibility of human cancer cells to H-1 viral infection

Cho, I-R,Kaowinn, S,Song, J,Kim, S,Koh, S S,Kang, H-Y,Ha, N-C,Lee, K H,Jun, H-S,Chung, Y-H Nature America, Inc. 2015 Cancer gene therapy Vol.22 No.5

Although H-1 parvovirus is used as an antitumor agent, not much is known about the relationship between its specific tropism and oncolytic activity. We hypothesize that VP2, a major capsid protein of H-1 virus, determines H-1-specific tropism. To assess this, we constructed chimeric H-1 viruses expressing Kilham rat virus (KRV) capsid proteins, in their complete or partial forms. Chimeric H-1 viruses (CH1, CH2 and CH3) containing the whole KRV VP2 domain could not induce cytolysis in HeLa, A549 and Panc-1 cells. However, the other chimeric H-1 viruses (CH4 and CH5) expressing a partial KRV VP2 domain induced cytolysis. Additionally, the significant cytopathic effect caused by CH4 and CH5 infection in HeLa cells resulted from preferential viral amplification via DNA replication, RNA transcription and protein synthesis. Modeling of VP2 capsid protein showed that two variable regions (VRs) (VR0 and VR2) of H-1 VP2 protein protrude outward, because of the insertion of extra amino-acid residues, as compared with those of KRV VP2 protein. This might explain the precedence of H-1 VP2 protein over KRV in determining oncolytic activity in human cancer cells. Taking these results together, we propose that the VP2 protein of oncolytic H-1 parvovirus determines its specific tropism in human cancer cells.

생쥐수정란에 대한 H - Y 항체처리가 산자의 성비에 미치는 영향

심호섭,고정재,김종배,박홍양,정길생 ( H . S . Shim,J . J . Ko,J . B . Kim,H . Y . Park,K . S . Chung ) 한국축산학회 1986 한국축산학회지 Vol.28 No.12

These experiments were carried out to control the sex of offsprings in mice by sexing embryos using immunological means prior to transfer to pseudopregnant recipients. H-Y antisera were prepared in inbred SD female rats by repeated immunization of testis supernatant and spleen cells from males of same strain. ELISA and indirect immunofluorescence test were used to detect H-Y antibody in antisera. Eight- to 16-cell mouse embryos were cultured in medium with H-Y antibody and complement (treated embryos) and in medium with BSA (control embryos). After 24-48 hr of culture, embryos were observed their morphological characteristics under the phase contrast microscope. Embryos developed to normal blastocyst were transferred to pseudopregnant recipients and sex of resultant offspring was investigated. The results obtained in these experiments were summarized as follows: 1. Production of H-Y antibodies in antisera obtained from immunized rats was confimed by ELISA and indirect immunofluorescence test. 2. Of 270 embryos treated with H-Y antibody and complement, 126 embryos (46.7%) were developed to normal blastocysts. 3. Following transfer of 126 blastocysts, 16 embryos (12.6%) were survived to term and 13 females (81.3%), significantly high ratio of female offspring, were produced.

성장단계별 농후사료 (濃厚飼料) 급여수준이 한우 육성비육우의 (育成肥育牛) 사료효율 , 산육능력 및 육질에 미치는 영향

강수원(S . W . Kang),장선식(S . S . Jang),정연후(Y . H . Chung),신기준(K . J . Shin),손용석(Y . S . Son) 한국영양사료학회 1995 韓國營養飼料學會誌 Vol.19 No.6

Enhanced ammonia dehydrogenation over Ru/La(x)-Al₂O₃ (x=0-50 mol%): Structural and electronic effects of La doping

Chung, D.B.,Kim, H.Y.,Jeon, M.,Lee, D.H.,Park, H.S.,Choi, S.H.,Nam, S.W.,Jang, S.C.,Park, J.H.,Lee, K.Y.,Yoon, C.W. Pergamon Press ; Elsevier Science Ltd 2017 International journal of hydrogen energy Vol.42 No.3

<P>Ru (1.0 wt% loaded)-based catalysts supported on La(x)-Al2O3 (x = 0,1, 5,10, and 50 mol%) were synthesized and characterized by X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET) measurement, X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), scanning transmission electron microscopy (STEM), and temperature programmed reduction (TPR). The as-prepared La(x)-Al2O3 materials were found to have increased amounts of the LaAlO3 phase as the La doping level (x) increased from 0 to 50 mol%. In addition to metal-to support interactions between Ru and Al2O3, the newly formed LaAlO3 phase in the Ru catalysts was proposed to interact strongly with Ru active sites based on the XRD, H-2-TPR and XPS results. The Ru/La(x)-Al2O3 catalysts were active for the dehydrogenation of ammonia, and among them, the Ru/La(10)-Al2O3 and Ru/La(50)-Al2O3 (or Ru/LaAlO3) catalysts exhibited superior performance with >96% conversions of ammonia at 550 degrees C. When an increased Ru content (2.0 wt%) was impregnated onto La(10)-Al2O3, the dehydrogenation activity was significantly improved with nearly 100% conversion (>95%) of ammonia at 500 degrees C. This catalyst further displayed an enhanced thermal stability towards ammonia decomposition with the GHSV(NH3) of 10,000 mL/g(cat)h at 550 degrees C for >120 h. The incorporated element La is thought to play an important role in enhancing metal-support interaction, ultimately facilitating ammonia dehydrogenation even at low temperatures. (C) 2016 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.</P>

Development of wall conditioning and impurity monitoring systems in Versatile Experiment Spherical Torus (VEST)

Lee, H.Y.,Yang, J.,Kim, Y.G.,Yang, S.M.,Kim, Y.S.,Lee, K.H.,An, Y.H.,Chung, K.J.,Na, Y.S.,Hwang, Y.S. North-Holland ; Elsevier Science Ltd 2016 Fusion engineering and design Vol.109 No.2

Wall conditioning and impurity monitoring systems are developed in Versatile Experiment Spherical Torus (VEST). As a wall conditioning system, a baking system covering the vacuum vessel wall partially and a glow discharge cleaning (GDC) system using two electrodes with dc and 50kHz power supplies are installed. The GDC system operates with hydrogen and helium gases for both chemical and physical desorption. The impurity monitoring system with residual gas analyzer (RGA), operating at <10<SUP>-5</SUP>Torr with a differential pumping system, is installed along with the optical emission spectroscopy (OES) system to monitor the hydrogen and impurity radiation lines. Effects of these wall conditioning techniques are investigated with the impurity monitoring system for ohmic discharges of VEST. The partial baking and He GDC show limited effects on plasma characteristics but sufficient H<SUB>2</SUB> GDC above 4h enables the longer plasma current duration up to ~15ms within 3h from the end of treatment.

1987년 한국에서 발생한 렙토스피라병의 혈청역학적 조사

이증훈,박영수,이우곤,김석용,정선식,우준희,박성광,박경희,송영욱,김선영,기정일,최두혁,강성귀,김주완,최강원,김우열,최명식,최인학,장우현,윤성열 대한감염학회 1988 감염 Vol.20 No.3

Human leptospirosis was an unfamiliar disease in Korea until 1984 that outbreak of leptospirosis occurred among farmers and soldiers after field works for harvesting rice. During that time, Lee and Jo confirmed the first Korean cases of leptospirosis by serological test, isolation of causative agent and autopy findings. Afterward several outbreaks occurred also during autumn especially after flood in every years and some characterisitcs of leptospirosis in Korea such as clinical manifestations, serotypes and seroepidemiological features has been revealed by many investigators. Because of the major mode of transmission between rodents and human is by direct contact with leptospiral urine of rodents or contaminated soil by the urine, leptospirosis in Korea has been primarily a disease of person in occupations heavily exposed to contaminated soil or infected urine such as farmer, army and etc. Therefore it seems that leptospirosis is one of the main communicable diseases to be controlled urgently in Korea, for an agricultural people account for almost half of total Korean people. For clarifying the seroepidemiological patterns of human leptospirosis in Korea by sex, month region and main reacting serovars of L. interrogans among acute febrile disease occurred in 1987, 1,773 patient's sers with acute febrile episodes were tested by microagglutination test using 19 representative strains of leptospiral serogroup as antigen. All of those sera were collected from 10 collaborative clinics located in Kyunggi, Kangwon, Chungbuk, Chungnam, Chonbuk, Chonnam province and Seoul. The results wee summerized as follows. 1) Among 1,773 sera of patients with acute febrile episodes, 219 (12.4%) were seropositive to L. interrogans, 487(27.5%) to R. tsutsugamushi, 241(13.6%) to R.typhi and 160(90.0%) to Hantaan virus. 2) Among seropositives to L.interrogans, the male outnumbered the female, 65% and 35%. 3) For age distribution, 26.9% of seropositives to L.interrogans were fifties, 19.6% were forties, 9.1% were sixties, 5.9% were thirties and 4.1% were twenties. 4) Eighty three percent of seropositives had occurred between September and October in 1987 with a peak in September. 5) Main leptospiral serovars reactive to patient's sera were Icterohaemorrhagiae(54.3%), Canicola(31.0%), CH-48(13.2%), Tarassovi(0.9%)and Cynopteri(0.5%). 6) For regional distribution, 65.8% of seropositives to L.interrogans were residents from Chonbuk, 12.3% were Chonnam, 7.3% were Chungnam, 5.5% were Kyunggi and 1.4% were Kangwon.

Heme oxygenase-1 mediates nicotine- and lipopolysaccharide-induced expression of cyclooxygenase-2 and inducible nitric oxide synthase in human periodontal ligament cells

Pi, S.-H.,Jeong, G.-S.,Oh, H.-W.,Kim, Y.-S.,Pae, H.-O.,Chung, H.-T.,Lee, S.-K.,Kim, E.-C. Blackwell Publishing Ltd 2010 Journal of periodontal research Vol.45 No.2

<P><I>Pi S-H, Jeong G-S, Oh H-W, Kim Y-S, Pae H-O, Chung H-T, Lee S-K, Kim E-C. Heme oxygenase-1 mediates nicotine- and lipopolysaccharide-induced expression of cyclooxygenase-2 and inducible nitric oxide synthase in human periodontal ligament cells. J Periodont Res 2010; 45: 177–183. © 2010 John Wiley & Sons A/S</I></P><P>Background and Objective: </P><P>Although heme oxygenase-1 (HO-1) plays a key role in inflammation, its anti-inflammatory effects and mechanism of action in periodontitis are still unknown. This study aimed to identify the effects of HO-1 on the proinflammatory mediators activated by nicotine and lipopolysaccharide (LPS) stimulation in human periodontal ligament (PDL) cells.</P><P>Material and Methods: </P><P>The production of nitric oxide (NO) and prostaglandin E<SUB>2</SUB> (PGE<SUB>2</SUB>) was evaluated using Griess reagent and an enzyme immunoassay, respectively. The expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and HO-1 proteins was evaluated by Western blot analysis.</P><P>Results: </P><P>Lipopolysaccharide and nicotine synergistically induced the production of NO and PGE<SUB>2</SUB> and increased the protein expression of iNOS, COX-2 and HO-1. Treatment with an HO-1 inhibitor and HO-1 small interfering RNAs blocked the LPS- and nicotine-stimulated NO and PGE<SUB>2</SUB> release as well as the expression of iNOS and COX-2.</P><P>Conclusion: </P><P>Our data suggest that the nicotine- and LPS-induced inflammatory effects on PDL cells may act through a novel mechanism involving the action of HO-1. Thus, HO-1 may provide a potential therapeutic target for the treatment of periodontal disease associated with smoking and dental plaque.</P>

Pretreatment HBeAg level and an early decrease in HBeAg level predict virologic response to entecavir treatment for HBeAg‐positive chronic hepatitis B

Kwon, J. H.,Jang, J. W.,Lee, S.,Lee, J.,Chung, K. W.,Lee, Y. S.,Choi, J. Y. Blackwell Publishing Ltd 2012 Journal of viral hepatitis Vol.19 No.2

<P><B>Summary. </B> There are few reports on hepatitis B e antigen (HBeAg) titres during nucleos(t)ide analogues treatment. We investigated the changes in HBeAg levels in patients treated with entecavir and the usefulness of HBeAg quantification for predicting antiviral response. Ninety‐five consecutive HBeAg‐positive patients treated with entecavir for more than 48 weeks were enrolled. Serum levels of hepatitis B surface antigen (HBsAg), HBeAg and HBV DNA were assessed at 4‐week intervals to week 24 and thereafter at 12‐week intervals. Virologic response (Y1VR) was defined as an undetectable HBV DNA level at week 48 of therapy. During 48 weeks, HBeAg and HBV DNA level decreased significantly in a biphasic manner and HBsAg level tended to decease. Fifty‐three patients (55.8%) attained Y1VR. Pretreatment HBeAg levels were significantly lower in the Y1VR group than in no Y1VR group. At week 4 and 12 of therapy, 25% and 41.4% of patients showed a decrease of HBeAg levels with >0.5 log<SUB>10</SUB> and >1.0 log<SUB>10</SUB> from baseline, respectively. These patients achieved more Y1VR than those with less decrease of HBeAg levels (97.7%<I>vs</I> 22.2% and 86.2%<I>vs</I> 29.3%, respectively). HBeAg level at week 12 had higher predictive values for Y1VR than HBV DNA level. Multivariate analysis revealed that a pretreatment HBeAg level of <360 PEIU/mL and the reduction in HBeAg level >1.0 log<SUB>10</SUB> at week 12 were associated with Y1VR. These results suggest that pretreatment HBeAg level and an early decrease in HBeAg level are useful measurements for predicting one‐year virologic response during entecavir treatment.</P>

15-Deoxy-Δ^12,14-prostaglandin J₂ induces p53 expression through Nrf2-mediated upregulation of heme oxygenase-1 in human breast cancer cells

Kim, D. H.,Song, N. Y.,Kim, E. H.,Na, H. K.,Joe, Y.,Chung, H. T.,Surh, Y. J. Informa Healthcare 2014 Free radical research Vol.48 No.9

<P>Heme oxygenase-1 (HO-1) is a stress-responsive enzyme that has antioxidant and cytoprotective functions. However, HO-1 has oncogenic functions in cancerous or transformed cells. In the present work, we investigated the effects of HO-1 on the expression of p53 induced by 15-deoxy-Δ<SUP>12,14</SUP>-prostaglandin J<SUB>2</SUB> (15d-PGJ<SUB>2</SUB>) in human breast cancer (MCF-7) cells. Treatment of MCF-7 cells with 15d-PGJ<SUB>2</SUB> led to time-dependent increases in the expression of p53 as well as HO-1. Upregulation of p53 expression by 15d-PGJ<SUB>2</SUB> was abrogated by si-RNA knock-down of HO-1. In MCF-7 cells transfected with HO-1 si-RNA, 15d-PGJ<SUB>2</SUB> failed to induce expression of p53 as well as HO-1. In addition, HO-1 inducers enhanced the p53 expression. We speculated that iron, a by-product of HO-1-catalyzed reactions, could mediate 15d-PGJ<SUB>2</SUB>-induced p53 expression. Upregulation of p53 expression by 15d-PGJ<SUB>2</SUB> was abrogated by the iron chelator desferrioxamine in MCF-7 cells. Iron released from heme by HO-1 activity is mostly in the Fe<SUP>2+</SUP> form. When MCF-7 cells were treated with the Fe<SUP>2+</SUP>-specific chelator phenanthroline, 15d-PGJ<SUB>2</SUB>-induced p53 expression was attenuated. In addition, levels of the Fe-sequestering protein H-ferritin were elevated in 15d-PGJ<SUB>2</SUB>-treated MCF-7 cells. In conclusion, upregulation of p53 and p21 via HO-1 induction and subsequent release of iron with accumulation of H-ferritin may confer resistance to oxidative damage in cancer cells frequently challenged by redox-cycling anticancer drugs.</P>