윤곽선 정보와 watershed segmentation을 이용한 영역 추출

이원효,설경호,염동훈,고기영,김두영 동아대학교 정보기술연구소 2003 情報技術硏究所論文誌 Vol.11 No.1

This paper proposed a extracting method of the region for image using segmentation and edge information. First proposed algorithm extract information using canny edge detector and the image was divided by watershed segmentation. And it extract the image with edge information by merging region. Finally we compare the proposed method with levelset method. In the result proposed method not only extract the image with accurate region but also reduce operation time.

우리나라 하수 및 폐수 처리 슬러지의 다환방향족탄화수소의 함량 : (2) Non-alkylated Polycyclic Aromatic Hydrocarbons

이강영,정창수,김영일,이현경,홍기훈 한국환경과학회 2005 한국환경과학회지 Vol.14 No.4

The 16 priority PAHs (Polycyclic Aromatic Hydrocarbons) designated by US Environmental Protection Agency were analyzed for some digested sludges from wastewater treatment plants in Korea. PAHs are an important group of organic contaminants present in sewage sludge due to their persistence and toxic potential. PAHs were extracted from freeze-dried sludges using a methylene chloride-methanol (2:1) mixture in a soxhlet extractor. The extracts were cleaned-up by silica gel/alumina combination column and subsequently fed into gas chromatograph/mass spectrometer (GC/MS) for determining PAH contents. The sum of the 16 PAHs in the sewage sludge varied from 534.8 to 5754.5 μg/kg, dry wt.. In the sewage sludge, phenanthrene appears as the most abundant PAHs, followed by naphthalene, pyrene, fluoranthene. Source of the investigated sewage sludges relatively predominated pyrogenic. PAHs levels of sewage sludges in Korea appeared to be lower than those in other countries.

Risperidone이 백서의 억제된 자발적 교대행동에 미치는 영향

이기철,이정호,김진규,정홍경,천강훈,류정환,최영민,전성일 대한생물치료정신의학회 1998 생물치료정신의학 Vol.4 No.2

Objectives : Recently, some case reports have shown that risperidone is effective to refractory obsessive-compulsive disorder. Possible explanations for efficacy of risperidone in refractory obsessive-compulsive disorder maybe due to that it is both serolonin and dopamine receptor antagonist. On the basis of serotonin-dopamine interaction hypothesis, a biological etiology of obsessive-compulsive disorder, the effect of risperidone was evaluated to the suppressed spontaneous alternation behaviour of animal model of obsessive-compulsive disorder in rats. Methods : The apparatus for spontaneous alternation behaviour was a black plexiglas T-maze with distinctive black and white goal boxes. Black gullotine doors separated the start box and the goal boxes from the main body of the T-maze Small cups were placed in the corners of both goal boxes(all arms measured 50×10cm) 24 hours prior to experiment, rats(Spraque-Dawley) were food-deprived. The food-deprived rats were put into T-maze, in which both goal boxes were baited with small amounts of chocolate milk. Each rat was given 2 set of 7 trials during which it was placed in the start box and allowed to choose the one of the goal boxes for checked. After baseline of the number of choices of spontaneous alternation behaviour was stabilized, the pontaneous alternation disrupted by nonselective 5-HT agonist, 5-MeODMT (1.25mg/kg/IP). The experimental animals were stratified into 5 groups, fluoxetine(10mg/kg/IP), risperidone(0.1mg/Kg/IP), haloperidol(0.1㎎/Kg/IP), fluoxetine(10mg/Kg) with haloperidol(0.1 mg/Kg), and saline(0.5cc/IP) control group. Each drugs were injected for 21 days as a chronic treatment. The protective effects were evaluated on the nest day of discontinuation of the each drugs among 5 groups. Results : 1) After 21 days of the drug treatment, the risperidone group and the fluoxetine group showed significant difference from the haloperidol group and the saline control group on the protection of the 5-MeODMT induced suppression of spontaneous alternation behaviour. 2) The fluoxetine group and fluoxetine with haloperidol group showed significant difference between before and after treatment in the protection of 5-MeODMT induced suppression of spontaneous alternation behaviour. The risperidone group also showed significant difference between before and after treatment in the protection of 5-MeODMT induced suppression of spontaneous alternation behaviour, but the protective effect of risperidone was superior to fluoxetine. Conclusion : These findings suggest that both risperidone and fluoxetine have a favorable effect in the protection of 5-MeODMT induced suppression of spontaneous alternation behaviour. We think that risperidone can be possible alternatives to SSRIs in the treatment of refractory obsessive-compulsive disorder in clinical situation.

立安散이 Angiogenesis 抑制棋戰에 미치는 影響

李紀龍,崔昇勳,安圭錫 대한한방종양학회 1998 대한한방종양학회지 Vol.4 No.1

This experimental study waas carried out to evaluate the effect of Yipahnasn on angiogenic inhibition mechanism. This study investigates the effects of Yipahnsan on angiogenic inhibition mechanism evaluate cell adhesive inhibition effect, DNA fragmantation analysis, nuclear condensation assay, FACScan analysis, angiogenic lumen formation assay, immunocytochemistry analysis, RT-PCR cor mRNA expression, western blot analysis, confocal analysis for Ca^2+influx. The results were wummarized as follows: 1. The cell adhesive inhibition ability was strongly increased from 5ug/ml on ECV304 cell line and ECVPAR cell line. 2. YY water extract caused G_0/G_1 arrest peak to existed on the ECV304 cell line. 3. YY water extract caused inhibition of proliferation and inducement of apoptosis on the collagen coated plate in ECV304 cell line. 4. YY water extract inhibited the lumen formation on the matrigel coated plate in ECV304 cell line. 5. YY water extract inhibited the expressions of LFA-1 and ELAM-1 on ECV304 cell line and ECVPAR cell line. 6. YY water extract inhibited the expressions of MMP-9 and uPA on ECV304 cell line and ECVPAR cell line. 7. YY water extract inhibited the expressions of integrin α_vβ_3 on ECV304 cell line and ECVPAR cell line. 8. YY water extract dereased the change of Ca^2+ in intracellular on ECV304 cell line and ECVPAR cell line. According to the results, Yipahnsan showed to be a key antagonist of integrin α_vβ_3 and to be induction of apoptosts by p53 through flow cytometry. This report also demonstrated that expressions of MMP-9 and uPA were blocked under the angiogenesis model. Thus, we suggested that Yipahnsan blocks angiogenesis by inducing apoptosis in ECV304 and ECVPAR cell lines, and another oriental herbal medicine that treats qi-stagnation and hlood-stasis type also has angiogenic inhibition effects.

MEMS 관성센서를 이용한 차량 자세인지 시스템

이정훈,김기택 강원대학교 정보통신연구소 2008 정보통신논문지 Vol.12 No.-

This study has shown the system acquiring information of vehicle posture and rotational direction to be used by the interlock with GPS Navigation system with MEMS inertial sensor(acceleration sensor and angular velocity sensor) and geomagnetic sensor. By using acceleration sensor like accelerometer this study was to know whether the vehicle was moving upward or downward by acquiring the information of pitch for vehicle during driving. Based on this information this was used to judge it fast whether the vehicle enter to elevated highway, uphill road, and downhill. In addition, this study has shown that it could acquire information of vehicle's rotational direction(yaw) and of moving direction by using the accelerometer and geomagnetic sensor. On the basis of it this study could find the escape of route fast during moving vehicle. When it uses various MEMS sensors, it can be more intelligent and effective navigation than the system using existing GPS only. Accordingly, this study was to suggest methods of sensing, data acquisition, data processing, and simulation for sensors.

교잡종 찰옥수수에 대한 연구 : Ⅰ. 찰옥수수 "연농×재래" 교잡종의 생육 및 수량특성 Ⅰ. Growth and Yield Characteristics in Waxy Maize Hybrid Yeonnong × Jaerae

이희봉,정재영,최현구,김기훈,김효기,주정일 충남대학교 농업과학연구소 2001 농업과학연구 Vol.28 No.2

재래종 찰옥수수 F₁ 교잡종에 대한 생육 및 수량비교를 위해 충남대학교 농과대학 실험포장에서 육성한 이들 교잡종의 식물학적 특성에 대한 연구 결과는 다음과 같다. 1. 간장은 대조구에 비해 대학찰×재래찰, FR805/1K₃×고령찰이 가장 크게 나타났으나, FR805/1K₃×대학찰, 찰옥 1호에서 대조구와 비슷하게 나타내었다. 2. 착수수는 간장에서와 마찬가지로 대학찰×재래찰, FR805/1K₃×고령찰이 대조구보다 높게 나타났으나 대학찰×재래찰, 찰옥 1호는 비슷하였다. 3. 분얼수는 각 교잡종들간에 큰 차이가 없었다. 4. 이삭의 길이는 대조구에 비해 교잡종들이 거의 2배이상의 크기를 나타내었다. 5. 이삭직경은 FR805/1K₃× 고령찰의 교잡종을 제외하고 대조구와 비슷하게 나타났다. 6. 이삭열수는 Daehakchal×1K₁/LE만이 대조구와 유의성을 나타내고 기타 교잡종은 대조구와 비슷하였다. In order to evaluate the characteristics of growth and yield in F_(1) waxy hybrids using Korean local maize lines, six hybrids including check, Chalok # 1, were planted at the experinental field of e Chungnam National University. Stem and ear heights of Yeonnong × Jaerae and FR805/IK_(3) × Koryong hybrids were higher than the check hybrid. Number of fillers per plant in all hybrids were similar as 0.3 to 0.5 per plant Ear length of waxy hybrids was longer than 15㎝ of check hybrid and ear diameter was also thicker than check except FR805/IK_(3) × Koryong waxy hybrid. A final result, Yeonnong × Jaerae among hybrids was comparatively superior to other hybrids in the test.

Clozapine이 백서의 억제된 자발적 교대 행동에 미치는 영향

천강훈,이기철,이정호,최영민,전성일,정홍경,류정환,최미 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.5

연구목적 : 강박장애의 생물학적 원인론중 세로토닌-도파민 상호작용 가설에 적합한 5-HT₂ 수용체와 도파민 D₂수용체를 동시에 차단하는 약물의 일종인 cloazpine과 세라토닌 재흡수 차단약물인 clomipramine, 그리고 도파민 D₂수용체 차단 약물인 haloperidol을 실험백서의 강박장애 동물모형에 투여하여 자발적 교대 행동에 미치는 영향을 비교, 측정하였다. 그 결과로서 1) 세라토닌 수용체와 도파민 수용체를 동시에 차단하는 약물의 효과를 확인하고, 2) 세라토닌과 도파민 수용체 동시차단 약물의 효과가 확인되면 임상적으로 기존의 세라토닌 재흡수 차단약물에 반응을 보이지 않는 강박장애 환자 치료에 세라토닌 수용체와 도파민 수용체를 동시에 차단하는 비전형적인 항정신병 약물들이 강박장애의 치료에 유용하게 사용될 수 있는 가능성을 제시하고자 하였다. 실험재료 및 연구방법 : 실험동물은 동일한 배에서 출생한 수컷 Spraque-Dawley 백서를 사용하였다. 자발적 교대 행동(spontaneous alternation behaviour : 이하 SAB)을 시험하기 위해 SAB의 기준치를 구하고 난 후, SAB을 차단하기 위한 방법으로 비선택적 세라토닌 순응제인 5-MeODMT(5-methoxy-N, N-dimethyltrytamine)를 투여하였다. 실험백서들을 clomipramine(5㎎/㎏), clozapine(10㎎/㎏), clozapine(20㎎/㎏), haloperidol(0.1㎎/㎏), 그리고 saline(0.2cc) 투여군으로 나누고 21일에 걸쳐서 복강내 주사하였다. 실험약물 투여가 끝난 다음날인 22일째에 5-MeODMT(5-methoxy-N, N-dimethyltryptamine : 1.25㎎/㎏/IP)를 각각의 실험동물군에게 투여하고 실험약물의 만성투여가 5-MeODMT 투여로 억제되는 SAB을 얼마나 호전시키는 지를 측정하였다. 모든 자료는 SPSS for window 7.5으로 통계처리 하였다. SAB의 기준치 5-MeODMT 투여로 억제되는 SAB의 효과, 실험약물의 만성투여에 따른 SAB의 변화는 일원분산분석을 사용하였으며, 사후검증은 Scheffe 검증을 적용하였다. 각 군내에서 기준치와 5-MeODMT 투여로 억제되는 SAB의 변화, 그리고 실험약물의 만성투여에 따른 SAB의 변화는 다중변량분석을 시행하였다. 결과 및 결론 : 역구결과 실험동물은 T-형 미로의 SAB에서 평균 1.33±0.36회의 훈련 기준치를 보였다. 5-MeODMT 주사후 , 실험동물의 SAB치는 3.12±0.63회를 보여 강박행동이 유발하였다. 21일간의 실험약물 투여후, 22일째에 5-MeODMT 주사에 대한 SAB의 반응은 clozapine 투여군과 clomipramine 투여군이 halo-peridol 투여군과 saline 투여군에 비하여 유의하게 낮은 수치를 보여 5-MeODMT투여로 억제된 SAB을 호전시키는 소견을 나타냈다. 각 실험동물군 내에서 각각의 SAB 훈련 기준치와 실험약물 투여 전후에 5-MeODMT 투여로 인한 SAB을 비교한 바, clomipramine 투여군은 약물투여 전에 5-MeODMT로 억제된 SAB이 약물투여후 의미있게 호전되었다. 한편, clozapine 투여군도 약물 투여전에 5-MeODMT로 억제된 SAB가 약물투여후 의미있게 호전되었으며, 이런 SAB의 호전은 clomipramine 투여군보다 우수하였다. 이상의 연구결과로 보아, 강박장애의 생물학적 병리론을 이해하는데 있어 세라토닌-도파민 상호작용 가설이 의미가 있음을 추정할 수 있었다. 향후, 강박장애의 임상치료에서 전형적인 SSRIs 치료에 반응치 않는 환자들에게 세라토닌-도파민 동시차단제인 비전형적인 약물의 단독투여를 시행하는 후속연구를 기대해 본다. Objectives : This study was designed to evaluate the role of the 5-HT₂and dopamine D₂antagonist on spontaneous alteration behaviour which is an animal modal of obsessive-compulsive disorder in rat. On the basis of serotonin-dopamine interaction hypothesis, the effect of clozapine was evaluated by applying the suppressed spontaneous alternation behaviour model. Methods : The apparatus for spontaneous alternation behaviour was a black plexiglas T-maze with distinctive black and white goal boxes. Black guillotine doors separated the start box and the goal boxes from the main body of the T-maze. Small cups of chocolate milk were placed in the corners of both goal boxes. At 24 hours prior to experiment, rats(Spraque-Dawley) food-deprived. The food-deprived rats were put into T-maze, in which both goal boxes were baited with small amounts of chocolate milk. Each rat was given 2 set of 7 trials during which it was placed in the start box and allowed to choose one of the goal boxes for each time. The mean number of choices until the occurrence of spontaneous alternation behaviour were checked. After baseline of the number of choices of spontaneous alternation behaviour was stabilized, the spontaneous alternation was disrupted by nonselective 5-HT agonist, 5-MeODMT(1.25mg/Kg/IP), The experimental animals were stratified into 5 groups :clomipramine(5mg/Kg/IP), clozapine(10mg/Kg/IP), clozapine(20mg/Kg/IP), haloperidol(0.1mg/Kg /IP), and saline(0.2cc/IP) control groups. They all went through 21 days fo treatment(intraperitoneal). The protective effects against the 5-MeODMT-induced disruption of spontaneous alternation behaviour were evaluated on the next day of drug treatment in each group. Results : 1) SAB was suppressed by 5-MeODMT injection. 2) After 21 days of the drug treatment, the clozapine and the clomipramine groups showed significant difference from the haloperidol and the saline control groups in the reversal of 5-MeODMT-induced suppression of spontaneous alternation behaviour. 3) The clozapine (20mg/kg/IP) group was superior to the clomipramine group in the protective effect of 5-MeODMT-induced suppression of spontaneous alternation behaviour. Conclusion : In clinical situation, the we think that atypical antipsychotic drugs those acting as serotonin and dopamine receptor antagonist with no extrapyramidal side effect can be beneficial to improve the symptoms of obsessive-compulsive disorder.

우리나라 하수 및 폐수 처리 슬러지의 유기인계 살충제의 함량

홍기훈,이강영,정창수,이현경 한국환경과학회 2006 한국환경과학회지 Vol.15 No.3

인공 치아우식 발생 모델에서 디지털 방사선 공제술을 이용한 인접면 치아우식증의 진단

박정훈,최용석,황의환,이기자,최삼진,박영호,김경숙,진현석,홍경원,오범석,박헌국 대한구강악안면방사선학회 2009 Imaging Science in Dentistry Vol.39 No.1

Purpose : The purpose of the experiment was to evaluating the diagnostic ability of dental caries detection using digital subtraction in the artificial caries activity model. Materials and Methods : Digital radiographies of five teeth with 8 proximal surfaces were obtained by CCD sensor (Kodak RVG 6100 using a size #2). The digital radiographic images and subtraction images from artificial proximal caries were examined and interpreted. In this study, we proposed novel caries detection method which could diagnose the dental proximal caries from single digital radiographic image. Results : In artificial caries activity model, the range of lesional depth was 572-1,374 μm and the range of lesional area was 36.95-138.52mm². The lesional depth and the area were significantly increased with demineralization time (p<0.001). Furthermore, the proximal caries detection using digital subtraction radiography showed high detection rate compared to the proximal caries examination using simple digital radiograph. Conclusion : The results demonstrated that the digital subtraction radiography from single radiographic image of artificial caries was highly efficient in the detection of dental caries compared to the data from simple digital radiograph.

분얼형 옥수수의 식물학적 특성과 RAPD 분석에 의한 근연관계

金哲民,李喜鳳,崔現求,鄭載永,金基薰 충남대학교 농업과학연구소 2001 농업과학연구 Vol.28 No.1

분얼형 옥수수 자석계통의 식물학적 특징, 유전적 거리를 조사하여 새로운 교잡종 육성을 위한 기초자료를 얻고자 실험 한 결과를 요약하면 다음과 같다. 1. 개화기에 있어서 조사된 20계통 중 K15가 가장 빨랐고 K07이 가장 늦은 개화소요일수를 보였다. 2. 간장과 착수고는 비슷한 경향으로 보며 K04가 가장 컸고, K15가 가장 작은 것으로 나타났다. 3. 상관 분석 결과 분열수는 개화기 및 출사기와 고도의 정의 상관을 보였으며, 100립중은 개화기, 이삭높이와 고도의 부의 상관을 가지고 있다. 4. RAPD 분석결과 17개의 BAND가 나타났고 이중 1개만이 monomolpic band를 보였으며, primer 당 평균 4개의 loci를 보였다. 5. 유전적 거리와 식물학적 특성간에는 개화기, 분얼수, 엽장과 100립중에서 관련이 있었다. This study was carried out to obtain a basic information for the development of a new corn hybrid with tillers. Materials used in this study were 20 lines having three to four tillers per plant including the PI213749 U S line with non-tillers. These 20 lines were compared for the botanical characteristics and genetic distances were measured using RAPD analysis. Flowering date of the K15 was very earlier. while the K07 was very late in flowering date. Stem height and ear height were similar except for KO4 and the K15. K06, K13 and K19 lines were appeared to be resistant to lodging due to decreased ear height. Number of tillers per plant of lines used were shown three to five on average. KO9 showed the highest kernel yield, while the K08 was low. Among characteristics measured tiller per plant and flowering dates, and silking dates showed a positive correlation, while 100 kernels weight, flowering date and ear height were shown a negative with tillers per plant. A total of 17 bands by RAPD analysis using four per primer were appeared and these lines were classified into three groups, especially the third group could be classified into of four sub-groups.