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( Ga Eun You ),( Bong Jun Jung ),( Hye Rim Kim ),( Han Geun Kim ),( Tae Rahk Kim ),( Dae Kyun Chung ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.10
Human skin is continuously exposed to ultraviolet (UV)-induced photoaging. UVA increases the activity of MMP-1 in dermal fibroblasts through mitogen-activated protein kinase (MAPK), p38, signaling. The irradiation of keratinocytes by UVA results in the secretion of the inflammatory cytokine, tumor necrosis factor-α (TNF-α), and the stimulation of MMP-1 in normal human dermal fibroblasts (NHDFs). Lipoteichoic acid (LTA) is a component of the cell wall of gram-positive Lactobacillus spp. of bacteria. LTA is well known as an anti-inflammation molecule. LTA of the bacterium Lactobacillus plantarum has an anti-photoaging effect, but the potential anti-photoaging effect of the other bacteria has not been examined to date. The current study showed that L. sakei LTA (sLTA) has an immune modulating effect in human monocyte cells. Our object was whether inhibitory effects of sLTA on MMP-1 are caused from reducing the MAPK signal in NHDFs. It inhibits MMP-1 and MAPK signaling induced by UVA in NHDFs. We also confirmed effects of sLTA suppressing TNF-α inducing MMP-1 in NHDFs.
항암화학요법을 받는 혈액암 환자의 증상경험, 사회적 지지와 삶의 질
김가은(Kim, Ga Eun),송주은(Song, Ju-Eun),유미애(You, Mi-Ae),박진희(Park, Jin-Hee) 대한종양간호학회 2022 Asian Oncology Nursing Vol.22 No.1
Purpose: The purpose of this study was to identify symptom experience, social support, quality of life (QOL), and the influencing factors on QOL of patients with hematologic malignancies undergoing chemotherapy. Methods: A cross-sectional study was conducted on 118 patients diagnosed with hematologic malignancies undergoing chemotherapy who visited an outpatient clinic in the Gyeonggi province. The Functional Assessment of Cancer Therapy-General (FACT-G), MD Anderson Symptom Inventory (MDASI), and the Multidimensional Scale of Perceived Social Support (MSPSS) were used to assess predictors and QOL. The data were analyzed using the Pearson correlation, t-test, ANOVA, and hierarchical multiple regression. Results: The mean score of QOL was (69.32±14.34). The type of hematologic malignancies and patient’s occupation were significantly associated with QOL in terms of demographic and disease/treatment-related characteristics. Symptom severity and symptom influence, and the support of family, friends, and medical staff had a significant association with QOL. The regression analyses showed that symptom severity (β=-.41, p<.001), friend support (β=.27, p=.001), and occupation (β=.14, p=.034) were significantly associated with QOL. Conclusion: The variables of symptom experience and social support must be acknowledged when improving QOL of hematologic malignancy patients undergoing chemotherapy. Greater focus on the reduction of symptom severity and increasing friend support could improve QOL among hematologic malignancy patients.
Jo, Eun Hye,Lee, Ga Young,Cho, Su Jin,Yoo, Hanna,Kim, On You,Seong, Kyeongyeol,Kang, Young Sun,Lee, Dongwon,Khang, Gilson National Taiwan University 2011 Biomedical engineering Vol.23 No.2
<P> The retinal pigment epithelium (RPE) closely interacts with photoreceptors in the maintenance of visual function. The native RPEs exist as a monolayer structure and have a mottled brown color due to the presence of melanin and other pigments including lipofuscin granules, which accumulate with age. In age-related macular degeneration (AMD), RPE's dysfunction and changes in Bruch's membrane occur. Thus, small intestine submucosa/poly(lactic-co-glycolic acid) (SIS/PLGA) film is a biomimetic transplant consisting of a layer of healthy RPE cells cultured on a support membrane. The goals of this study were to evaluate the effects of attachment and proliferation of RPEs on SIS/PLGA films. Porcine SIS is an acellular tissue and widely used as a biomaterial without immunorejection responses, whereas PLGA is a biodegradable synthetic polymer with acceptable mechanical strength and well-controlled degradation rate. We fabricated SIS/PLGA films using 20 wt% of SIS. We measured MTT to confirm cellular adhesion of cell number attached on film at 1, 3, 5, and 7 days. Morphology of cellular adhesion on films was confirmed by scanning electron microscopy at 1, 3, and 7 days. Further, reverse transcription polymerase chain reaction (RT-PCR) was conducted to confirm messenger RNA expression of RPE65 as RPE's marker and expression of cytokeratin, and RPE65 were determined by AEC immunocytochemical staining. These results suggest that SIS provides suitable surface to RPEs. </P>
Song, Eun Joo,Kang, Juhye,You, Ga Rim,Park, Gyeong Jin,Kim, Youngmee,Kim, Sung-Jin,Kim, Cheal,Harrison, Roger G. The Royal Society of Chemistry 2013 Dalton transactions Vol.42 No.43
<P>A new metal ion sensor that contains quinoline and pyridylaminophenol has been synthesized. In acetonitrile and in the presence of Zn<SUP>2+</SUP>, the sensor fluoresces. In contrast, in aqueous solutions the sensor fluoresces in the presence of Cd<SUP>2+</SUP>. The fluorescence of the molecule is selective for Zn<SUP>2+</SUP> or Cd<SUP>2+</SUP> and shows no fluorescence with other metal ions. The crystal structure of the Cd<SUP>2+</SUP> complex shows coordination through the amide and phenol oxygens, as well as the amine nitrogen of the sensor. The sensor also acts as a colorimetric sensor for Co<SUP>2+</SUP> by changing color from colorless to yellow. The color change is selective for Co<SUP>2+</SUP> and is not observed with other metal ions. The unique combination of pyridine and phenol groups with quinoline results in the properties of this sensor.</P> <P>Graphic Abstract</P><P>A new metal ion sensor based on quinoline fluoresces in organic solvents with Zn<SUP>2+</SUP>, fluoresces in aqueous solutions with Cd<SUP>2+</SUP> and changes color in organic solvents with Co<SUP>2+</SUP>. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c3dt51635k'> </P>
최가은(Ga-Eun Chae),박유경(You-Kyung Park),최준호(Jun-Ho Choi) 한국화재소방학회 2022 한국화재소방학회 학술대회 논문집 Vol.2022 No.춘계
전 세계적으로 석유사용량 급증으로 인한 연료 부족 사태와 내연기관 자동차의 배기가스 배출로 인해 환경오염 등의 문제가 발생하고 있다. 이에, 많은 나라들이 친환경 정책의 일환으로 내연기관 자동차의 배출가스 규제를 강화하면서 하이브리드자동차, 전기자동차, 수소자동차 등 친환경 자동차 개발에 관한 관심이 증가하였다. 친환경 자동차 시장에서는 전기자동차의 점유율이 증가하고 있는데 현재 52% 점유율을 차지하고 있다. (Sun et al., 2020) 전기자동차의 증가와 더불어 전기자동차 화재 또한 매년 증가하고 있어 그 위험성이 대두되었다. 본 연구에서는 국내외 사례연구와 문헌조사를 통해 전기 자동차 화재 원인을 분석하고 실험연구 동향을 정리하였다. 전기자동차 화재는 정지 상태에서의 자연발화, 충전 중 과충전으로 인한 발화, 교통사고와 같은 고속충돌로 인한 충격이 대표적인 원인으로 나타나고 있으며, 주요 원인으로는 리튬 이온 배터리(LIB)의 열과 가스의 배출, 열충격, 기계적 충격, 전기 남용으로 정리할 수 있다. (Lecocq et al., 2012) 대부분의 전기 자동차 화재 위험은 LIB와 연관되어 있어 미국, 스웨덴, 중국 등에서는 사고사례를 바탕으로 관련 이론 연구와 표준메뉴얼에 따른 실물실험을 통한 화재 위험 평가를 실시하였다. 국내에서도 전기자동차의 화재 성상 실험 및 LIB 화재 위험성에 관한 연구를 진행하였다. (Lim, 2021) 다만, 국내외 공통적으로 전기 자동차 화재 메커니즘 및 화재 진압에 관한 연구는 미비한 것으로 근본적인 화재 예방에 관한 연구 뿐만 아니라 건축물에 의무적으로 설치되는 전기자동차 급속충전기와 완속충전기가 설치되는 주차장의 설치기준 등에 대한 연구도 뒤따라야 할 것으로 판단된다.
실크/PLGA 필름에서 실크 함량이 망막색소 상피세포의 부착 및 증식 거동에 미치는 영향
조은혜(Eun Hye Jo),김수진(Soo Jin Kim),조수진(Su Jin Cho),이가영(Ga Young Lee),김온유(On You Kim),이은용(Eun Yong Lee),조원형(Won Hyung Cho),이동원(Dong Won Lee),강길선(Gil Son Khang) 한국고분자학회 2011 폴리머 Vol.35 No.4
망막 조직공학을 위한 생체 재료는 기계적 안전성, 생체적합성, 낮은 분해속도 등을 포함하여 in vivo에서 잠재적인 유용성을 위한 몇 가지 중요한 특징이 입증되어야 한다. 실크 필름 생체재료는 이러한 기능적인 요구에 맞게 디자인되었다. 0, 10, 20, 40, 및 80 wt%의 실크가 함유된 천연/합성물질과 하이브리드화된 silk/PLGA 필름을 용매 증발법으로 제조하였다. 1, 2, 및 3일 후에 부착된 세포 수를 확인하기 위해 MTT 분석을 하였고 SEM을 통해 필름에 부착된 세포 모폴로지를 확인하였다. 또한, mRNA 발현정도를 알아보기 위해 retinal pigment epithelium(RPE) 세포의 프라이머인 RPE65를 사용하여 RT-PCR을 실시하였고 RPE 세포의 특정 단백질인 cytokeratin의 발현을 확인하고 세포의 증식을 비교하기 위해 면역화학염색을 실시하였다. 본 실험을 통해 실크/PLGA 필름에서 20∼40 wt%실크를 함유한 경우에 RPE 세포의 부착과 증식에 가장 좋은 영향을 미치는 것을 확인하였다. Biomaterials for retinal tissue engineering must demonstrate several critical features for potential utility, including mechanical integrity, biocompatibility, and slow biodegradation. Silk film biomaterials were designed and characterized to meet these functional requirements. We prepared natural/synthetic hybrid silk/PLGA films using 0, 10, 20, 40, and 80 wt% of silk by a solvent evaporation method. MTT assay was used to confirm the number of cells attached on film at 1, 2, and 3 days, respectively. The morphology of cellular adhesion on films was also confirmed by scanning electron microscope (SEM). RT-PCR was conducted to confirm mRNA expression of retinal pigment epithelium(RPE) using RPE65 as a RPEs marker and the expression of cytokeratin were determined by immunofluorescence staining. We confirmed that the silk/PLGA film of 20∼40 wt% silk was superior for the adhesion and proliferation of RPEs.