Dissipation pattern and risk quotients assessment of amisulbrom in Korean melon cultivated in plastic house conditions

Kabir, Md. Humayun,Abd El-Aty, A. M.,Rahman, Md. Musfiqur,Chung, Hyung Suk,Lee, Han Sol,Park, Sun-Hyang,Hacımü,ftü,of,lu, Fazil,Chang, Byung-Joon,Shin, Ho-Chul,Shim, Jae-Han Springer-Verlag 2017 Environmental monitoring and assessment Vol.189 No.6

A quick and effective methodology for analyzing dinotefuran and its highly polar metabolites in plum using liquid chromatography-tandem mass spectrometry

Rahman, Md. Musfiqur,Abd El-Aty, A.M.,Kabir, Md. Humayun,Chung, Hyung Suk,Lee, Han Sol,Hacımü,ftü,of,lu, Fazil,Jeong, Ji Hoon,Chang, Byung-Joon,Shin, Ho-Chul,Shim, Jae-Han Elsevier 2018 Food chemistry Vol.239 No.-

<P><B>Abstract</B></P> <P>A simple and effective method was developed for analyzing dinotefuran and its three metabolites (MNG, UF, and DN) in plum using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Due to the polarity and high water miscibility, dinotefuran and some of its metabolites (especially DN) have some limitations to be extracted with acetonitrile and salt following the “QuEChERS” sample preparation methodology. Alternatively, the samples were extracted with methanol, and purified with dispersive-solid phase extraction procedure (d-SPE) using primary secondary amine (PSA) and C<SUB>18</SUB> sorbents after filtration, and mass up. Due to the suppression effect originated from plum matrix, matrix-matched calibration curves, which provided good linearity with coefficient of determination (<I>R<SUP>2</SUP> </I>)≥0.998, were used for quantification of all analytes. Blank plum samples fortified with 2 spiking levels (10×LOQ and 50×LOQ) yielded satisfactory recoveries for all tested analytes in the range of 83.01 to 110.18% with relative standard deviation (RSD)≤8.91. The method was successfully applied to field-incurred plum samples and dinotefuran and all metabolites were positively detected and quantified. In conclusion, we suggest that the method can be expanded to polar compounds having solvent and partitioning problems in any of the versions of QuEChERS.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A simultaneous method was developed for dinotefuran and its highly polar metabolites in plum. </LI> <LI> Mass-up was introduced instead of salting out for exact quantitation. </LI> <LI> Internal standard is not needed for the newly developed methodology. </LI> <LI> The method can be used either as it is or with slight modification in other matrices. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Uptake of the veterinary antibiotics chlortetracycline, enrofloxacin, and sulphathiazole from soil by radish

Chung, Hyung Suk,Lee, Young-Jun,Rahman, Md. Musfiqur,Abd El-Aty, A.M.,Lee, Han Sol,Kabir, Md. Humayun,Kim, Sung Woo,Park, Byung-Jun,Kim, Jang-Eok,Hacımü,ftü,of,lu, Fazil,Nahar, Nilufar,Sh Elsevier BV 2017 Science of the Total Environment Vol.605 No.-

<P><B>Abstract</B></P> <P>Veterinary antibiotics are available for uptake by the plants through sources such as manure, irrigation, and atmospheric interaction. The present study was conducted to estimate the half-lives of three veterinary antibiotics, chlortetracycline (CTC), enrofloxacin (ENR), and sulphathiazole (STZ), in soil and experimentally explore their uptake from contaminated soil to radish roots and leaves. Samples were extracted using a modified citrate-buffered version of the quick, easy, cheap, effective, rugged, and safe “QuEChERS” method followed by liquid chromatography coupled with tandem mass spectrometric analysis (LC–MS/MS) in the positive ion mode. Good linearity was observed for the three tested antibiotics in soil and plants (roots and leaves) with high coefficients of determination (<I>R</I> <SUP>2</SUP> ≥0.9922). The average recovery rates at two spiking levels with three replicates per level ranged between 77.1 and 114.8%, with a relative standard deviation (RSD)≤19.9% for all tested drugs. In a batch incubation experiment (<I>in vitro</I> study), the half-lives of CTC, ENR, and STZ ranged from 2.0–6.1, 2.2–4.5, and 1.1–2.2days, respectively. Under greenhouse conditions, the half-lives of the three target antibiotics in soil with and without radishes were 2.5–6.9 and 2.7–7.4; 4.7–16.7 and 10.3–14.6; and 4.4–4.9 and 2.5–2.8days, respectively. Trace amounts of the target antibiotics (CTC, ENR, and STZ) were taken up from soil <I>via</I> roots and entered the leaves of radishes. The concentration of CTC was lower than 2.73%, ENR was 0.08–3.90%, and <1.64% STZ was uptaken. In conclusion, the concentrations of the tested antibiotics decreased with time and consequently lower residues were observed in the radishes. The rapid degradation of the tested antibiotics in the present study might have only little impact on soil microorganisms, fauna, and plants.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A method to estimate the half-life of veterinary antibiotics in soil was developed. </LI> <LI> Veterinary antibiotic uptake rate by radish from contaminated soil was determined. </LI> <LI> The half-lives of antibiotics in soil with and without radishes did not differ. </LI> <LI> Trace antibiotic residue levels moved to roots and leaves from contaminated soil. </LI> <LI> We elucidate residual antibiotic transplantation and antibiotic tolerance in soil. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

고랭지 배추 재배지에서 사탕무씨스트선충의 물과 토양을 통한 포장 간 분산

권오경(Oh-Gyeong Kwon),신진희(Jin-Hee Shin),F. Md. Kabir,이재국(Jae-Kook Lee),이동운(DongWoon Lee) 한국원예학회 2016 원예과학기술지 Vol.34 No.1

Sugar beet cyst nematode, Heterodera schachtii , causes serious economic losses worldwide in Brassicaceae crops. In 2011, this nematode was first found in highland vegetable cultivation areas in Korea, and thereafter spread to the surrounding healthy Chinese cabbage fields. However, little has been documented on the biological and ecological characteristics of the sugar beet nematode in highland vegetable cultivation areas. In this study the dispersal of the sugar beet cyst nematode was examined, focusing on spreading through soil and/or water infested with the nematode. When farmers with work boots trampled on Chinese cabbage fields for 10 minutes, the number of cysts recovered from the soil attached to the working differed depending on the research sites. Under irrigation conditions of 2, 4, and 8 liters of water per m², the amounts of soils attached on the bottom of the work boots and the number of healthy cysts in the soils increased significantly with the increase in soil moisture, although the total number of cysts, eggs, and juveniles did not increase significantly. After driving on the farm road adjacent to cabbage fields infested with the sugar beet cyst nematode, cysts were also recovered from the soil attached to the vehicle’s tires, suggesting that the sugar beet cyst nematode can spread to new fields through soil carried by vehicles and by farmers. An excavator and a motor truck could deliver 41 kg and 224 g, respectively, of soil on the shovel shoes and the wheels to other locations during the Chinese cabbage harvest, suggesting that farming equipment and transportation vehicles may be vital means by which the cyst nematode spreads to noninfested fields in the highland area of Korea. Runoff water also contained cysts, whose amounts increased after water irrigation onto the ridges to simulate rainy conditions, with no significant difference in the number of cysts with increasing amounts of irrigation water. All of these results confirmed that the sugar beet cyst nematode spreads through soil attached to work boots, harvesting tools, and transportation vehicles, especially during the harvest time, and through runoff water on rainy days. These observations suggest that personal sanitization and cleaning of working tools and vehicles are one of the most important means to prevent the dispersal of the sugar beet cyst nematode in fields.

온도와 뿌리 추출물이 사탕무씨스트선충, Heterodera schachtii의 부화와 발육에 미치는 영향

( F. Md. Kabir ),신진희 ( Jin Hee Shin ),권오경 ( Oh Gyeong Kwon ),이동운 ( Dong Woon Lee ) 한국토양동물학회 2015 한국토양동물학회지 Vol.19 No.1

Sugar beet cyst nematode, Heterodera schachtii, has recently been identified as a severe pest of Chinese cabbage field in the highland area of Korea. In this experiment, we tried to find out the effect of temperature and Chinese cabbage root extract in egg hatching as well as egg development of the nematode. Five different temperature conditions, 10, 15, 20, 25 and 30℃, with growth chamber were used to find the suitable hatching temperature where 25℃ showed the maximum egg hatching rate. However, 10 and 15℃ showed negligible hatching rate. In contrast, 20, 30 and 40 days-aged-Chinese cabbage root extracts were used for hatching in 25℃ climate chamber, where 20 days extract showed the highest hatching rate and 40 days presented the lowest egg hatching effect. To justify furthermore, egg development stages test carried out with 20 and 25℃ temperatures, where the egg development stage perform faster in 20 than 25℃.