개구리 皮膚의 伸張度와 X線 全身照射가 이에 미치는 影響

李基錫,朱永恩 慶北大學校 醫科大學 1975 慶北醫大誌 Vol.16 No.1

개구리皮膚의 伸張度와 X-線照射가 이에 미치는 影響을 알기 爲하여 腹部 및 背部皮膚에서 各各 矢狀 및 橫斷切片을 作成하여 길이-張力曲線中 彈性의 限界內에서 Hooke의 法則에 따르는 部位에서 Young率을 求 하고 1,000R의 X-線全身照射를 한 後 1,3,5,7 및 10日에서 各各 皮膚切片의 伸張度를 求하여 서로 比較 하였던 바 다음과 같은 結果를 얻었다. 1. 正常개구리의 腹部皮膚의 矢狀 및 橫斷切片의 Young率은 各各 4.09×10 exp (7) dyne/㎠ 및 3.99×10 exp (7) dyne/㎠이었다. 背部皮膚에서 얻은 矢狀 및 橫斷切片의 Young率은 各各 3.69×10 exp (7) dyne/㎠ 및 5.81×10 exp (7) dyne/㎠이었다. 2. X-線 全身照射를 한 群에서 腹部皮膚에서는 그 矢狀切片의 Young率은 照射 3日에서 5.38×10 exp (7) dyne/㎠으로서 가장 높고 그 後는 漸次 낮아지는 傾向을 나타내었고 照射 10日에서는 4.20×10 exp (7) dyne/㎠으로서 正常値에 가까워지는 傾向을 나타내었다. 橫斷切片에서는 照射 3日 및 5日에서 Young率이 各各 4.55×10 exp (7) dyne/㎠ 및 4.50×10 exp (7) dyne/㎠으로서 가장 높고 그後는 第10日까지 次次 낮아진듯하나 그 傾向이 矢狀切片에서 처럼 뚜렷하지 않았다. 3. X-線 全身照射로서 背部皮膚에서는 그 矢狀切片의 Young率은 照射 第3日 및 5日에서 各各 4.25×10 exp (7) dyne/㎠ 및 4.30×10 exp (7) dyne/㎠으로서 높아지는 傾向을 나타내고 그後는 漸次 낮아져서 第10日에서는 正常値와 비슷하게 되는 傾向을 나타내었다. 橫斷切片에서의 Young率도 照射後 第3日 및 5日에서 各各 6.02×10 exp (7) dyne/㎠및 6.00×10 exp (7) dyne/㎠으로서 正常値보다 若干 높은듯 하고 그後는 낮아지는 傾向을 보이나 그 程度가 矢狀切片에서 처 럼 뚜렷하지 못하였다. In an attempt to observe the changes of the distensibility of the frog skin by whole body X-irradiation, the sagittal and transverse strips were excised from the abdominal and back skin of the frog (Rana nigromaculata) following whole body X-irradiation with 1,000R. Young Modulus of the strip was calculated from the length-tension curve of each sample, and a comparison was made with the non-irradiated control group. Experiments were performed on 1,3,5,7 and 10 days after the irradiation, and the results thus obtained are summarized as follows: 1. Young Modulus of the sagittal and transverse strips of the abdominal skin of the normal frog was 4.09×10 exp (7) dyne/㎠ and 3.99×10 exp (7) dyne/㎠, respectively. Young Modulus of the back skin of the normal frog was 3.69×10 exp (7) dyne/㎠ in the sagittal strip, and 5.81×10 exp (7) dyne/㎠ in the transverse strip. 2. Young Modulus of the sagittal strip of the abdominal skin in the irradiated group was the highest (5.38×10 exp (7) dyne/㎠) on the 3rd post-irradiation day, and a tendency of gradual decrease to the normal value on the 10th post-irradiation day was observed. In the transverse strip of the abdominal skin in the irradiated group, the highest values of Young Modulus were observed on the 3rd(4.55×10 exp (7) dyne/㎠) and 5th(4.50×10 exp (7) dyne/㎠) post-irradiation day, and a tendency of gradual decrease till 10th day was observed, but the tendency was not as prominent as in the sagittal strip. 3. A tendency of the elevated values of Young Modulus on the 3rd(4.25×10 exp (7) dyne/㎠) and 5th(4.30×10 exp (7) dyne/㎠) post-irradiation day with the decreasing values to the normal value on the 10th day was observed in the sagittal strip of the back skin of the irradiated frog. In the transverse strip of the irradiated frog, Young Modulus showed a slight increase on the 3rd(6.02×10 exp (7) dyne/㎠) and 5th(6.00×10 exp (7) dyne/㎠) day after the irradiation, but a tendency of decrease was observed thereafter.

Influence of heat‑treated Al-Si coating on the weldability and microstructural inhomogeneity for hot stamped steel resistance nut projection welds

Eun‑Joon Chun,Sung‑Sang Lim,Young‑Tae Kim,Ki‑Sung Nam,김영민,Young‑Whan Park,Siva Prasad Murugan,Yeong‑Do Park 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.1

Resistance nut projection weldability of Al–Si coated hot stamped steel (HSS) was investigated under the viewpoint of weldablecurrent range and joint strength (pull-out load). The microstructural inhomogeneities in the welds were also studied inorder to elucidate the factors affecting the joint strength of the welds. The weldability of the given Al–Si coated HSS wascompared with the weldability of an identical HSS without the Al–Si coating (Al–Si coating was polished out) and Zn coateddual phase steel. The weldable current range of Al–Si coated HSS was found to be narrower than that of the other materials. Furthermore, the average pull-out load within the weldable current range of the Al–Si coated HSS was the lowest among thethree materials. The reason for poor weld mechanical property of the Al–Si coated hot-stamped steel was attributed to themicrostructural inhomogeneities such as unmixed Al–Si coating layer at the edge of the nugget and the second phase Fe3(Al,Si) intermetallic compound. The formation of Fe3(Al, Si) phase was attributed to the solidification segregation of Al and Siduring the weld solidification and was confirmed with the numerical analysis of solidification segregation.

Hair growth promoting effects of different alternating- current parameter settings are mediated by the activation of Wnt/β-catenin and MAPK pathway

( Ki Min Sohn ),( Kwan Ho Jeong ),( Jung Eun Kim ),( Young Min Park ),( Hoon Kang ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2

Background: There are clinical studies demonstrating the positive effect of electrical stimuli on hair regrowth. However, the underlying mechanism and optimal parameter settings are not clarified. Objectives: To investigate the effects of different parameter settings of electrical stimuli on hair growth by examining changes in human dermal papilla cells (hDPCs) in vitro and by observing molecular changes in animal tissue. Methods: In vitro, cultured hDPCs were electrically stimulated with different parameter settings. Cell proliferation was measured by MTT assay. The Ki67 expression was measured by immunofluorescence. Hair growth-related gene expressions were measured by RT-PCR. In animal model, different parameter settings were applied to the shaved dorsal skin of rabbit for 8 weeks. Expression of hair-related genes in the skin of rabbit was examined by RT-PCR.Results: At low voltage power (3.5V) and low frequency (1MHz or 2MHz) alterating current, in vitro proliferation of hDPCs was successfully induced. A significant increase in Wnt/モ-catenin, Ki67, p-ERK and p-AKT expression was observed. In animal model, hair regrowth was observed in the entire stimulated areas and expression of hair-related genes in the skin significantly increased. Conclusion: There are optimal conditions for electric stimulated hair growth and they might be different in the cells, animal, and human tissue. Electric stimuli induces mechanisms such as activation of Wnt/モ-catenin and MAPK pathway in hair follicles.

서울의 PPNG 발생 빈도(1991-1992)

김재홍,윤기범,박평원,김영진,전경민,김영태,김중환,곽호,구상완,송민석,유옥,지혜구,김동원,문상은,박영립,정승호,성범진,성순제,엄주용,황정열,이기홍,이주협,전태진 대한화학요법학회 1994 대한화학요법학회지 Vol.12 No.1

The prevalence of PPNG among pretreated gonorrhea cases isolated at the STD clinic of Choong-Ku Public Health Center in Seoul has been studied and reported annually since 1981. In 1991, 123 strains of N.gonorrhoeae were isolated, among which 58(47.1%) were PPNG. In 1992, 98 starains of N.gonorrhoeae were isolated, among which 51(52.0%) were PPNG. In all, 109(49.3%) strains were found to be PPNG among 221 strains isolated between 1991-1992. The prevalence of PPNG in Seoul showed increased tendency till 1989, thereafter, it has been stationary or slightly decreasing.

Therapeutic Effects of Recombinant Human Epidermal Growth Factor (rhEGF) in a Murine Model of Concurrent Chemo- and Radiotherapy-Induced Oral Mucositis

RYU, Seung-Hee,KANG, Ki Mun,MOON, Soo Young,CHAI, Gyu Young,HONG, Joon Pio,CHO, Kyoung-Oh,KANG, Mun-Il,CHOI, Eun Kyung,LEE, Sang-wook Journal of Radiation Research Editorial Committee 2010 JOURNAL OF RADIATION RESEARCH Vol.51 No.5

<P>Concurrent chemotherapy with radiotherapy (CCRT) has been applied for the treatment of advanced stage of head and neck cancer patients. However CCRT is associated with several complications including mucositis, dermatitis, stomatitis, etc. This study was conducted to evaluate the therapeutic effect of systemically administrated recombinant human epidermal growth factor (rhEGF) in CCRT-induced oral mucositis in a mouse model. Oral mucositis was induced in male BALB/c mice through combination treatment with cisplatin (11 mg/kg, i.p.) and irradiation (17 Gy) of the head and neck area. rhEGF (1.0 mg/kg/day for consecutive 3 days) was administered systemically, and the therapeutic effect was determined by histological evaluation of the oral mucosa. To elucidate optimal dose of rhEGF on CCRT-induced mucositis, various concentrations (0.04–3 mg/kg) of rhEGF were injected for 3 days. Systemic rhEGF administration accelerated the recovery of body weight. Histologically, rhEGF-treated mice showed significantly increased epithelial cell layer thickness, basal cell number, and expression of Ki-67 compared to control mice. Most effective dose was 1 mg/kg among other doses tested. Systemic administration of 1 mg/kg of rhEGF reduces the severity of oral mucositis induced by CCRT in a mouse model, suggesting that rhEGF can be used for treating CCRT-induced mucositis during the cancer treatment.</P>

물질남용 치료프로그램 후 재범여부에 대한 예비적 추적조사 연구

김현수,조선미,오은영,임기영,정영기,윤웅장,이영미 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.6

연구목적: 본 연구는 물질사범에게 시행되는 수강명령 프로그램의 효과를 재범률을 중심으로 살펴보고자 한 것이다. 방 법: 이 연구의 대상집단은 보호관찰소에서 의뢰받은 물질남용자집단이다. 이들은 법원으로부터 보호관찰, 수강명령, 치료명령, 사회봉사 등을 부과받았다. 총 104명의 대상자집단을 치료프로그램을 이수한 수강군과 비수강군으로 나누었으며 이들에 대한 재범률을 조사하였다. 결 과: 프로그램을 이수한 수강군이 비수강군에 비해 재범률이 낮았다(p<.05). 또한 단기 수강군과 장기수강군의 재범률의 차이를 조사하였는데 대상자 집단의 수가 적기 때문에 장기 수강군의 효과가 통계적으로 입증되지는 않았다. 결 론: 우리는 물질남용자에 대한 정책이 보다 교육적으로 변화할 것을 본 논문을 통하여 제시하려 하였으며 정부 및 법무부는 물질남용자에게 교육과 치료를 제공해주어야만 한다. Objectives: Using recidivism rate, we investigated the outcome after our substance abuse treatment program had been implemented. Methods: Our target population was substance abuse criminals referred by probational office. They either received the parole and probation order, treatment order or social service order from the court. We divided 104 substance abuse criminals into treatment group and non-treatment group. We examined their recidivism rate. Results: We reached a conclusion that treatment group had lower recidivism rate than non-treatment group(p<.0.5). We examined the effectiveness of short-term and long-term programs. However, no effectivess of long-term program was found because the sabject number was too small. Conclusion: We suggest that the strategies for substance abuse should be changed to more educative ones. The Government and Ministry of Justice must provide education and treatment to the substance abuse criminals.

아리스톨로크산 함유 생약제에 대한 안전성평가연구 : 3개월간 반복투여독성시험을 통한 신장독성평가

황명실,박미선,문지영,이지선,염영나,이효민,신동환,강진석,윤은경,최미나,육미영,장동덕,길광섭,김승희,양기화 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

본 실험에서는 아리스톨로크산을 함운하고 있는 생약제중 하나인 마두령(.4risfoforfioe JTurruf)의 90일 반복투여독성시헌을 실f,」하였다. 마두령에서 아리스톨로크산을 정량분석한 결과 마두령 단일 건조븐말의 경운 2.112mg/g, 잉상에서 처방되고 있는 마든령복방 (마두령외 9가지 생약제 복합처방) 건조분말꼭 경무에는 0.066m9/5으로 각각 검출되었다. 본 실험에서 용량설정은 마두령 처리군 (저용량 군; 21.3m9/k9/day (임상용량), 중용량군; 2t3mgfg/day, 고용량군; 2430mg딘g b.w./day), 마두령복방 처리군 (427mg/kg b.w./da?; 임상용량) 및 positive control (아리스톨로크산) 처리군 (저용량군;0.05mgag/da17, 중용량군; 0.Smg/kg/day, 고용량군; 5mg./kg/dal·1으료 하여 랫드에게 경구투여하였다. 마두령단방 처궈근 및 마두령복방 처리군에서 저용량군과 중응량군에서는 잉상관찰 및 생화학적 분석에서 패조군과 유의적인 차이가 없었다. 그러나 마두령단방 고용량근에서는 간, 신장의 상대적인 무계가 증가되었고, 신장의 유두이행상피증식 및 암종이 관찰되었으며, 위에서는 편평세포암종이 관찰되었다. 하자만 간손상이나 간암은 유발하지는 않는 것으로 판단되었다. 결론끌으로 본 실험의 결과에서는 아리스톨로크산 함유 생약제인 마두령이 임상용량인 저응량 처리군에서는 독성을 나타내지 않았으나, 고용쏭으로 장기 복용시켰을 깅우 설치류의 전위부위 및 신장에서 독성이 있음을 알 수 있었다. Chines herbs nephropathy (CHN) has been described in young women who had taken a slimming pills containing some chines herbs. Aristolochic acid (AA), suspected substance as the causal factor of CHN, is known a carcinogen. The Aristolochiae fructus (fruit of Aristolochia contorta) was used in Korean Traditional Medicine consists of appropriate amounts of mixed natural products. Subchronic toxicity of A. furctus containing aristolochic acid was investigated in SD rats. The body weight and clinical signs were observed after orally administration of A. futctus at doses of 21.2, 213, and 2130 mg/kg/day; mixture (including 9 other herbs) at dose of 427 mg/kg/day; aristolochic aicd (Ⅰ+Ⅱ) at doses of 0.05, 0.5, and 5 mg/kg/day for 3 months. At the end of the treatment, high dose treated animals revealed a deficit in final body weight about 25% compared with that of control. Organ weights of kidney, liver, testis, or ovary were increased with dose dependent manner. No changes considered to be due to the administration of A. furctus, mixture or aristolochic acid were in hematological and clinical study. However, significant changes at histopathological study of kidney and stomach were boserved in high-dose treated groups. In conclusion, for human health safety it needs appropriate regulatory actions regarding the use of natural herbal medicines known or suspected of containing aristolochic acid.

개 아토피성 피부염 장기치료 1례

박은정,윤기영,박정호,이왕희,박성준 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2011 動物醫科學硏究誌 Vol.18 No.1

A 7-year-old male castrated Shih-tzu dog was referred to the Veterinary Medical Teaching Hospital of Chungnam National University for severe pruritus. On physical examination were confirmed systemic erythema and alopecia, crust on the neck and flank region. Tape strip test identified numerous degenerative neutrophils and severe Malassezia infection. Treatment for severe pruritus was applied with systemic antihistamine therapy and topical therapy with antimicrobial shampoos. For the finding of appropriate treatment of atopic dermatitis, it was chosen six immune-related treatments. And clinical sign scores were evaluated by investigator with CADESI-03 after each application of six treatments. Application of human immunoglobulin G and leflunomide showed the remarkable regression of lesions.

Shigella flexneri에 의한 집단발병의 역학적 양상

기현균,김선희,기혜영,서진종,하동룡,김은선,정재근,김성한,이복권 대한감염학회 2004 감염과 화학요법 Vol.36 No.3

배경 : 국내에서 Shigella flexneri에 의한 감염으로 인한 집단발병은 2000년도에 국립보건원에 집단발병례가 보고된 이후 보고되지 않았으나 최근 저자 등은 S. flexneri에 의한 대규모 집단발병을 경험하여 역학적인 양상을 기술하였다. 방법 : OO회사의 직원 및 구내식당을 이용한 사람 및 가족들을 대상으로 증상여부 및 2002년 12월 23일 이후 식당이용 여부와 취식음식에 대하여 설문조사를 실시하였으며 검체를 확보하여 미생물학적 검사를 실시하였다. 결과 : 조사대상 총 258명 중 총 110명에서 증상이 발생하였으며 증상발생일의 분포는 단일정점을 보였다. 확진자는 82명으로 이중 81명은 OO의 식당을 이용한 사람이었고 1명은 식당에서 직접 조리과정에 참여한 사람이었다. 식사여부와 증상여부의 관계를 분석한 결과 특히 24일의 취식여부의 상대위험도가 가장 높았다(RR=6.46, 3.56≤95% CI≤11.75). 항균제 내성 양상은 ampicillin 내성균주가 82균주(100.0%) ampicillin-sulbactam 내성균주가 55균주(67.1%), amoxicillin-clavulanic acid에 대한 내성균주가 62균주(74.4%), trimethoprim-sulfamethoxazole 내성균주가 82균주로 나타났다(98.8%). 분리된 균주간의 연관성을 보기 위하여 실시한 PFGE 결과 본 집단발병에서 확인된 균주는 동일한 양상을 보였으나 비슷한 시기에 분리된 균주와는 서로 다른 양상을 보였다. 결론 : 본 집단발병은 국내에서 발생한 S. flexneri에 의한 집단발병 중 가장 대규모 집단발병이었다. 본 집단발병에서 확인된 균주간의 PFGE양상이 동일하였으나 기존에 국내에서 분리된 균주의 PFGE양상과는 다른 양상을 보였다. 따라서 이러한 상황을 감안한다면 S. flexneri 및 설사환자에 대한 감시체계를 강화해야 할 것이다. Background : Most of the shigellosis outbreak in Korea was caused by Shigella sonnei since late 1990's. In contrast, outbreak by Shigella flexneri were rare since late 1990's. Currently. we experienced the shigellosis outbreak by S. flexneri and described the results of investigation. Methods : We recruited employees from company "A" who had a meal at least once at the company's cafeteria from Dec 23th, 2002 to Dec 26th, 2002. We surveyed the symptoms, food items, and history of travel of eligible persons and their family members. For the microbiological examination, we collected specimen from eligible persons and their family. Collected specimens were cultured for bacteriologic agents and viruses. Epidemiological relationship among the isolates were analyzed by pulsed field gel electrophoresis (PFGE). Results : Among the eligible persons, one hundred ten were symptomatic (110/258, 42,6%). Eighty-two were confirmed bacteriologically. Day of symptom onset showed a unipolar pattern. Diarrhea was the most common symptom among the symptomatic patients (110/110, 100%). The other symptoms included abdominal pain (81.8%), tenesmus (70.0%), headache (63.6%), nausea (61.8%), febrile sense (59.1%), and vomiting (24.5%). Lunch on 24th Dec was the most significant risk factor of the outbreak (RR=6.46, 3.56≤95% Cl≤11.75). All isolates of the outbreak has the same pattern on PFGE analysis and the PFGE pattern was not similar compared with other S. flexneri isolates from Korea. Conclusion : This is the largest and unique outbreak by S.flexneri since 2000 in Korea. The outbreak has the same origin according to the PFGE pattern and epidemic characteristics. Considering rarity of S.flexneri among shigellosis in Korea, surveillance for S.flexneri should be enhanced.

개의 아토피 피부염에 대한 Leflunomide의 임상적 적용 1례

홍은지,윤기영,서경원,최호정,정성목,송근호,조종기,박성준 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2012 動物醫科學硏究誌 Vol.19 No.1

A Shih-tzu(8-year-old, castrated male) was referred to the Veterinary Medical Teaching Hospital of Chungnam National University with the history of relapsing pruritus, recurrent dermatitis. On the physical examination, generalized erythema, otitis externa, alopecia and lichenification were observed. Using Favrot's criteria for diagnishing atopic dermatitis, 7 criteria were satisfied out of 8 criteria. After treatment for infection, intradermal skin test was performed(positive allergen : Df, Dp). And then immunotherapy was performed, but clinical signs were not getting better. Next treatment of glucocorticoid shown little improvement of clinical signs, and adverse effect(diarrhea) was shown after cyclosporine application. So application of leflunomide which is used for human atopic dermatitis treatment in some case was planned. After 6 months of application of leflunomide, clinical signs were decreased gradually.