http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Oh, Jisun,Daniels, Gabrielle J,Chiou, Lawrence S,Ye, Eun-Ah,Jeong, Yong-Seob,Sakaguchi, Donald S Wiley 2014 Biotechnology Journal Vol.9 No.7
<P>Adult hippocampal progenitor cells (AHPCs) are generally maintained as a dispersed monolayer population of multipotent neural progenitors. To better understand cell-cell interactions among neural progenitors and their influences on cellular characteristics, we generated free-floating cellular aggregates, or neurospheres, from the adherent monolayer population of AHPCs. Results from in vitro analyses demonstrated that both populations of AHPCs were highly proliferative under maintenance conditions, but AHPCs formed in neurospheres favored differentiation along a glial lineage and displayed greater migrational activity than the traditionally cultured AHPCs. To study the plasticity of AHPCs from both populations in vivo, we transplanted green fluorescent protein (GFP)-expressing AHPCs via intraocular injection into the developing rat eyes. Both AHPC populations were capable of surviving and integrating into developing host central nervous system, but considerably more GFP-positive cells were observed in the retinas transplanted with neurosphere AHPCs, compared to adherent AHPCs. These results suggest that the culture configuration during maintenance for neural progenitor cells (NPCs) influences cell fate and motility in vitro as well as in vivo. Our findings have implication for understanding different cellular characteristics of NPCs according to distinct intercellular architectures and for developing cell-based therapeutic strategies using lineage-committed NPCs.</P>
Wicking Property of Graft Material Enhanced Bone Regeneration in the Ovariectomized Rat Model
김승현,안태호,한명호,배춘식,Daniel S. Oh 한국조직공학과 재생의학회 2018 조직공학과 재생의학 Vol.15 No.4
BACKGROUND: Recruitment and homing cells into graft materials from host tissue is crucial for bone regeneration. METHODS: Highly porous, multi-level structural, hydroxyapatite bone void filler (HA-BVF) have been investigated to restore critical size bone defects. The aim was to investigate a feasibility of bone regeneration of synthetic HA-BVF compared to commercial xenograft (Bio-Oss). HA-BVF of 0.7 mm in average diameter was prepared via template coating method. Groups of animals (n = 6) were divided into two with normal (Sham) or induced osteoporotic conditions (Ovx). Subsequently, subdivided into three treated with HA-BVF as an experiment or Bio-Oss as a positive control or no treatment as a negative control (defect). The new bone formation was analyzed by micro-CT and histology. RESULTS: At 4 weeks post-surgery, new bone formation was initiated from all groups. At 8 weeks post-surgery, new bone formation in the HA-BVF groups was greater than Bio-Oss groups. Extraordinarily greater bone regeneration within the Ovx-HA group than Sham–Bio-Oss or Ovx–Bio-Oss group (p\0.05). CONCLUSION: This study suggests that the immediate wicking property of HA-BVF from host tissue activates a natural healing cascade without the addition of exogeneous factors or progenitor cells. HA-BVF may be an effective alternative for repairing bone defects under both normal and osteoporotic bone conditions.
Jörg U. Schmohl,Martin Felices,Felix Oh,Alexander J. Lenvik,Aaron M. Lebeau,Jayanth Panyam,Jeffrey S. Miller,Daniel A. Vallera 대한암학회 2017 Cancer Research and Treatment Vol.49 No.4
Purpose The selective elimination of cancer stem cells (CSCs) in tumor patients is a crucial goal because CSCs cause drug refractory relapse. To improve the current conventional bispecific immune-engager platform, a 16133 bispecific natural killer (NK) cell engager (BiKE), consisting of scFvs binding FcRIII (CD16) on NK cells and CD133 on carcinoma cells, was first synthesized and a modified interleukin (IL)-15 crosslinker capable of stimulating NK effector cells was introduced. Materials and Methods DNA shuffling and ligation techniques were used to assemble and synthesize the 1615133 trispecific NK cell engager (TriKE). The construct was tested for its specificity using flow cytometry, cytotoxic determinations using chromium release assays, and lytic degranulation. IL-15–mediated expansion was measured using flow-based proliferation assays. The level of interferon (IFN)- release was measured because of its importance in the anti-cancer response. Results 1615133 TriKE induced NK cell–mediated cytotoxicity and NK expansion far greater than that achieved with BiKE devoid of IL-15. The drug binding and induction of cytotoxic degranulation was CD133+ specific and the anti-cancer activity was improved by integrating the IL-15 cross linker. The NK cell–related cytokine release measured by IFN- detection was higher than that of BiKE. NK cytokine release studies showed that although the IFN- levels were elevated, they did not approach the levels achieved with IL-12/IL-18, indicating that release was not at the supraphysiologic level. Conclusion 1615133 TriKE enhances the NK cell anti-cancer activity and provides a self-sustaining mechanism via IL-15 signaling. By improving the NK cell performance, the new TriKE represents a highly active drug against drug refractory relapse mediated by CSCs.
( Jong Min Kim ),( Tae Sung Han ),( Myoung Hwan Kim ),( Daniel S. Oh ),( Seong Soo Kang ),( Gon Hyung Kim ),( Tae Yub Kwon ),( Kyo Han Kim ),( Kyu Bok Lee ),( Jun Sik Son ),( Seok Hwa Choi ) 한국조직공학·재생의학회 2012 조직공학과 재생의학 Vol.9 No.3
The goal of this study was to develop a bioactive hydroxyapatite (HA) scaffold as a calcium phosphatebased bioceramic using drug-loaded polymeric microspheres for bone regeneration. Dexamethasone (DEX) as a model bioactive molecule and poly (lactic-co-glycolic acid) (PLGA) microspheres as a carrier were employed. Polyethyleneimine was coated on DEX-loaded PLGA microsphere surfaces, resulting in a net positively-charged surface. With such modification of the PLGA microsphere surfaces, DEX-loaded PLGA microspheres were immobilized on the negatively charged HA scaffold surfaces. The release profile of DEX over a 4-week immersion study indicated an initial burst release followed by a sustained release. In vivo evaluation of the defects filled with DEX-loaded HA scaffolds indicated that new bone formation was enhanced when compared to defects that were either unfilled or filled only with HA scaffold. This innovative platform for bioactive molecule delivery more potently induced osteogenesis in vivo, which may be exploited in implantable bone graft substitutes for stem cell therapy or improved in vivo performance.
You, Changkook,Lee, Moon-Hwan,Lee, Hyo-Jin,Han, Myung-Ho,Kwon, Tae-Yub,Kim, Kyo-Han,Oh, Daniel S. Elsevier 2017 CERAMICS INTERNATIONAL Vol.43 No.4
<P><B>Abstract</B></P> <P>Cellular responses such as adhesion, spreading, growth and proliferation can be altered by macro and micro pores as well as surface patterns on its surface. In this study, the scaffold was prepared with interconnected macro pores by the polymeric sponge method and with interconnected micro pores by BCP slurry coating with different particle sizes on sintered porous body and necking sintering process. Two particle sizes for micro pores, 200–400nm and 600–800nm, were coated on a pre-sintered porous body. A combined macro and micro pore structure of the BCP scaffold can improve osteoblast cellular activity. <I>In vitro</I> study of MG63 cells suggest that the modified scaffolds improved cell response such as cell spreading, proliferation and differentiation. The scaffold that has a uniform distribution of micro pores ranging 1~5µm in diameter on its surface yields the highest rate of cellular response.</P>