Dronedarone Attenuates Ang II-Induced Myocardial Hypertrophy Through Regulating SIRT1/FOXO3/PKIA Axis

Cheng Chen,Song Hu,Heng-Jing Hu,Zhi-Xuan Liu,Xin-Teng Wu,Tao Zou,Hua Su 대한심장학회 2024 Korean Circulation Journal Vol.54 No.4

Background and Objectives: Long-term pathological myocardial hypertrophy (MH) seriously affects the normal function of the heart. Dronedarone was reported to attenuate left ventricular hypertrophy of mice. However, the molecular regulatory mechanism of dronedarone in MH is unclear. Methods: Angiotensin II (Ang II) was used to induce cell hypertrophy of H9C2 cells. Transverse aortic constriction (TAC) surgery was performed to establish a rat model of MH. Cell size was evaluated using crystal violet staining and rhodamine phalloidin staining. Reverse transcription quantitative polymerase chain reaction and western blot were performed to detect the mRNA and protein expressions of genes. JASPAR and luciferase activity were conducted to predict and validate interaction between forkhead box O3 (FOXO3) and protein kinase inhibitor alpha (PKIA) promoter. Results: Ang II treatment induced cell hypertrophy and inhibited sirtuin 1 (SIRT1) expression, which were reversed by dronedarone. SIRT1 overexpression or PKIA overexpression enhanced dronedarone-mediated suppression of cell hypertrophy in Ang II-induced H9C2 cells. Mechanistically, SIRT1 elevated FOXO3 expression through SIRT1-mediated deacetylation of FOXO3 and FOXO3 upregulated PKIA expression through interacting with PKIA promoter. Moreover, SIRT1 silencing compromised dronedarone-mediated suppression of cell hypertrophy, while PKIA upregulation abolished the influences of SIRT1 silencing. More importantly, dronedarone improved TAC surgery-induced MH and impairment of cardiac function of rats via affecting SIRT1/FOXO3/PKIA axis. Conclusions: Dronedarone alleviated MH through mediating SIRT1/FOXO3/PKIA axis, which provide more evidences for dronedarone against MH.

Distinctive Roles of Wnt Signaling in Chondrogenic Differentiation of BMSCs under Coupling of Pressure and Platelet-Rich Fibrin

Cheng Baixiang,Feng Fan,Shi Fan,Huang Jinmei,Zhang Songbai,Quan Yue,Tu Teng,Liu Yanli,Wang Junjun,Zhao Ying,Zhang Min 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.4

BACKGROUND: Although newly formed constructs of feasible pressure-preadjusted bone marrow mesenchymal stem cells (BMSCs) and platelet-rich fibrin (PRF) showed biomechanical flexibility and superior capacity for cartilage regeneration, it is still not very clear how BMSCs and seed cells feel mechanical stimuli and convert them into biological signals, and the difference in signal transduction underlying mechanical and chemical cues is also unclear. METHODS: To determine whether mechanical stimulation (hydrostatic pressure) and chemical cues (platelet-rich fibrin, PRF) activate canonical or noncanonical Wnt signaling in BMSCs, BMSCs cocultured with PRF were subjected to hydrostatic pressure loading, and the activation of the Wnt signaling molecules and expression of cartilage-associated proteins and genes were determined by western blotting and polymerase chain reaction (PCR). Inhibitors of canonical or noncanonical Wnt signaling, XVX-939 or L690,330, were adopted to investigate the role of Wnt signaling molecules in mechanically promoted chondrogenic differentiation of BMSCs. RESULTS: Hydrostatic pressure of 120 kPa activated both Wnt/b-catenin signaling and Wnt/Ca2? signaling, with the the maximum promotion effect at 60 min. PRF exerted no synergistic effect on Wnt/b-catenin signaling activation. However, the growth factors released by PRF might reverse the promotion effects of pressure on Wnt/Ca2? signaling. Real-time PCR and Western blotting results showed that pressure could activate the expression of Col-II, Sox9, and aggrecan in BMSCs cocultured with PRF. Blocking experiment found a positive role of Wnt/b-catenin signaling, and a negative role of Wnt/ Ca2? signaling in chondrogenic differentiation of the BMSCs. Mutual inhibition exists between canonical and noncanonical Wnt signaling in BMSCs under pressure. CONCLUSION: Wnt signaling participates in the pressure-promoted chondrogenesis of the BMSCs co-cultured with PRF, with canonical and noncanonical pathways playing distinct roles during the process.

Identification of Transmission Line Lightning based on HHT

Cheng Zixia,Pei Haowei,Teng Chenyuan 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.8

Lightning groundwire or tower without fault, back striking, shielding failure without fault and shielding failure with fault are simulated and analyzed in this paper based on the electromagnetic transient simulation software of ATP-EMTP. The results show that the current waveform of lightning strikes the ground or tower without fault is generated by the coupling of lighting current from lighting groundwire and oscillates around the axis of the steady state. Back striking contains processes of couple and flashover, its current waveform has characteristics of reverse polarity pulse and sudden current drop. The current of shielding failure without fault is the lighting current. The current waveform of shielding failure with fault is characterized by the truncation. On this basis, the Hilbert Huang transform (HHT) was introduced to do further analysis of the simulation results. The solution of end effect is also proposed in this paper. And thus the marginal spectrum was obtained by dealing with the simulated current waveform of lightning stroke. Then, calculating the energy distribution in different frequency bands and normalizing them. Therefore, a method for recognizing 4 types of lightning stroke is proposed which is on the basis of the energy proportion in different frequency bands. In addition, a large number of simulations are conducted to verify the effectiveness of this method by changing the parameters of the lightning current, grounding resistance and lightning distance.

Effect of design parameters on the anti-penetration properties of space armor

Teng, Tso-Liang,Shih, Ta-Ming,Lu, Cheng-Chung Techno-Press 2008 Structural Engineering and Mechanics, An Int'l Jou Vol.28 No.6

New types of armor, including space armor, multiple-layered armor, composite armor and modular armor have been successfully developed and installed on the armored vehicles of several nations. The protective capability of armor against penetration is established. Of developed composite armor, space armor has a simple structure and is easy to produce and can be produced at low cost. This study uses the finite element package DYTRAN and the pre and post processor PNTRAN to elucidate the ballistic resistance and penetration of space armor. Factors such as armor thickness, space between armors and projectile profile are considered. A technique for simulating the protection afforded by armor and supporting the design of space armor is developed.

Does the Fit of Managerial Ability with Firm Strategy Matters on Firm Performance

CHENG, Teng Yuan,LI, Yue-Qi,LIN, Yu-En,CHIH, Hsiang-Hsuan Korea Distribution Science Association 2020 The Journal of Asian Finance, Economics and Busine Vol.7 No.4

The study aims to answer why the previous studies find the positive or insignificant effect of the CEO's abilities on firm performance. Using 34,285 CEO-firm-year panel data from the U.S. publicly traded firms drawn from the BoardEx and EXECUXOMP database during from 1992 to 2014, the results show that the fit of the CEO's generality or specialist ability with firm strategy matters on firm performance and risk. This study computes a discrete STRATEGY composite measure to construct firm strategy types, such as Prospect or Defend and use CEOs' résumés to construct an index of general skills that are transferable across firms and industries. The results find that generalist CEOs are more suitable for prospectors than specialist CEOs. Firm performance is much better when specialist CEOs work for Defenders. Although the firm performance is better too for the generalist CEOs who fit for the Prospect strategy, the firm's risk is up too. The result suggests that firms need to consider their chosen business strategy to recruit and select CEOs Our findings provide direct evidence that the match between CEO's ability and the firm's strategy is crucial to firm performance and risk.

Global incidence and prevalence of nonalcoholic fatty liver disease

Margaret LP Teng,Cheng Han Ng,Daniel Q. Huang,Kai En Chan,Darren JH Tan,Wen Hui Lim,Ju Dong Yang,Eunice Tan,Mark D. Muthiah 대한간학회 2023 Clinical and Molecular Hepatology(대한간학회지) Vol.29 No.-

Nonalcoholic fatty liver disease (NAFLD) is a leading cause of liver disease worldwide. The estimated global incidence of NAFLD is 47 cases per 1,000 population and is higher among males than females. The estimated global prevalence of NAFLD among adults is 32% and is higher among males (40%) compared to females (26%). The global prevalence of NAFLD has increased over time, from 26% in studies from 2005 or earlier to 38% in studies from 2016 or beyond. The prevalence of NAFLD varies substantially by world region, contributed by differing rates of obesity, and genetic and socioeconomic factors. The prevalence of NAFLD exceeds 40% in the Americas and South-East Asia. The prevalence of NAFLD is projected to increase significantly in multiple world regions by 2030 if current trends are left unchecked. In this review, we discuss trends in the global incidence and prevalence of NAFLD and discuss future projections.

Effect of design parameters on the anti-penetration properties of space armor

Tso-Liang Teng,Ta-Ming Shih,Cheng-Chung Lu 국제구조공학회 2008 Structural Engineering and Mechanics, An Int'l Jou Vol.28 No.6

New types of armor, including space armor, multiple-layered armor, composite armor and modular armor have been successfully developed and installed on the armored vehicles of several nations. The protective capability of armor against penetration is established. Of developed composite armor, space armor has a simple structure and is easy to produce and can be produced at low cost. This study uses the finite element package DYTRAN and the pre and post processor PNTRAN to elucidate the ballistic resistance and penetration of space armor. Factors such as armor thickness, space between armors and projectile profile are considered. A technique for simulating the protection afforded by armor and supporting the design of space armor is developed.

Recent progresses in marine microbial-derived antiviral natural products

Yun‑Fei Teng,Li Xu,Mei‑Yan Wei,Chang‑Yun Wang,Yu‑Cheng Gu,Chang‑Lun Shao 대한약학회 2020 Archives of Pharmacal Research Vol.43 No.12

Viruses have always been a class of pathogenicmicroorganisms that threaten the health and safety of humanlife worldwide. However, for a long time, the treatment ofviral infections has been slow to develop, and only a fewantiviral drugs have been using clinically. Compared withthese from terrestrial environments, marine-derived microorganismscan produce active substances with more novelstructures and unique functions. From 2015 to 2019, 89antiviral compounds of 8 structural classes have been isolatedfrom marine microorganisms, of which 35 exhibit anti-H1N1 activity. This review surveys systematically marinemicrobial-derived natural products with antiviral activityand illustrates the impact of these compounds on antiviraldrug discovery research.

Hepatocellular carcinoma surveillance in non-alcoholic fatty liver disease – who and how?

Margaret LP Teng,Darren Jun Hao Tan,Cheng Han Ng,Daniel Q. Huang 대한간학회 2023 Clinical and Molecular Hepatology(대한간학회지) Vol.29 No.2

Low-temperature deposition of nanocrystalline Al₂O₃ films by ion source-assisted magnetron sputtering

Ding, Ji Cheng,Zhang, Teng Fei,Mane, Rajaram S.,Kim, Kwang-Ho,Kang, Myung Chang,Zou, Chang Wei,Wang, Qi Min Elsevier 2018 Vacuum Vol.149 No.-

<P><B>Abstract</B></P> <P>In this paper, bipolar pulse reactive magnetron sputtering with ion source assisted deposition method has been utilized for the deposition of nanocrystalline alumina (Al<SUB>2</SUB>O<SUB>3</SUB>) films at the temperature of 300 °C onto silicon (111) wafers, and cemented carbide substrates. The influence of ion source power, i.e. 0, 1.0, 1.5 and 2.0 kW on the structure, morphology, compressive stress and mechanical properties of the Al<SUB>2</SUB>O<SUB>3</SUB> films were investigated. In absence of ion source power assistance, an amorphous Al<SUB>2</SUB>O<SUB>3</SUB> film was dominant at deposition temperature of 300 °C. With ion source assisted deposition, the crystalline γ-Al<SUB>2</SUB>O<SUB>3</SUB> films were obtained at the same conditions, suggesting an importance of ion source power in crystallinity development of metal oxide films obtained from magnetron sputtering deposition method. Images of surface morphology clearly demonstrated the difference in granular sizes of film surfaces prepared with and without ion source powers. With increasing ion source power from 1.0 to 2.0 kW, the micro-hardness and compressive stress of the films were increased from 7 GPa to 13 GPa and 0.3 GPa to 1.1 GPa, respectively. Results revealed that the reactive magnetron sputtering with the ion source assisted deposition was a simple and effective way to prepare nanocrystalline γ-Al<SUB>2</SUB>O<SUB>3</SUB> films at low temperature.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Al<SUB>2</SUB>O<SUB>3</SUB> films were synthesized by Ar ion source-assisted bipolar pulsed sputtering technique. </LI> <LI> Nanocrystalline Al<SUB>2</SUB>O<SUB>3</SUB> films were obtained at relatively low deposition temperature (300 °C) and low bias voltage conditions. </LI> <LI> The orientation and microstructure of the films can be adjusted by altering the ion source power. </LI> <LI> The synthesis approach of crystalline Al<SUB>2</SUB>O<SUB>3</SUB> films in this study is promising for large-scale industrial applications. </LI> </UL> </P>