건강식품 바이탈 및 비지놀의 抗疲勞 效果에 관한 연구

정경수,김혜경,김희수,김계양,곽영숙,김학범 충남대학교 약학대학 의약품개발연구소 1993 藥學論文集 Vol.9 No.-

As a result of extensive research efforts on the oriental medicinal herbs, some of their interesting biological activities have been elucidated. In the present study, antifatigue effects of two natural food supplements consisting of oriental herbs, Vital and Vigilor, were investigated using the ICR mice. The mice were fed with antibiotic-free normal mouse feed containing Vital or Vigilor for 14,28, or 31 days and then subjected to forced swim tests. In a test carried out in melting-ice water, Vital and Vigilor significantly increased the exhaustion times of the 28 day-fed mice by 26.9 % (p < 0.01) and 16.2% (p < 0.05), respectively. However, in the other test performed at 15±0.5℃ using 31 day-fed mice, Vigilor failed to increase the exhaustion time significantly (at p< 0.05), while Vital showed antifatigue effect by increasing the swimming time by 29.9% (p<0.01). Neither of Vital nor Vigilor exerted antifatigue effects in a swim test carried out on day 14 after the initiation of feeding. These results suggest that Vital and Vigilor exhert antifatigue effets when administered for a sufficient period of time by enhancing the health of the host animals.

광에너지 총량에 따른 광중합 콤포짓트레진과 콤포머의 중합도에 관한 연구

황경란,이용근,임범순,김철위 대한치과기재학회 2000 대한치과재료학회지 Vol.27 No.4

Dental composite resin is filler added, particle reinforced composite material, and the usage range had been enlarged in the posterior restoration through improvement of physical properties. Recently, resin modified glassionomer and compomer are used for various purposes. The degree of polymerization of cross-liked polymer has great importance in determining the mechanical properties and clinical availability of materials. Residual carbon-carbon double bonds have been implicated in causing reduced wear resistance, strength and color stability. The degree of polymerization is an important factor for longevity of a restoration. However the higher the degree of polymerization in resin composites, the higher the shrinkage will result. It has been elucidated that the degree of polymerization was significantly affected by the intensity of the curing light, wavelength of light and irradiation time. In this experiment, 16 kinds of restorative materials such as five kinds of universal composite resin, five kinds of condensable composite resin, two kinds of flowable composite resin, and four kinds of compomer were used. This study examined the effect of light intensity and irradiation time on the polymerization behavior such as the heat release during polymerization and the rate of polymerization by DSC (differential scanning calorimeter), and the degree of double bond conversion by FT-IR. With DSC, the irradiation modes were three different light intensity of 300, 400, 600 ㎽/㎠ and for 40 seconds. With FT-IR, the levels of irradiation energy were 1,500, 40,000, 8,000, 16,000 and 24,000 mJ/㎠ by combining the light intensity of 300, 400, 600 ㎽/㎠ and irradiation time of 5, 10, 20 and 40 seconds. From this experiment, The following results were obtained : 1.In the DSC result, heat of polymerization of composite resin was 23.22∼55.17J/gram and exothermic energy of compomer was 26.50∼52.68 J/gram 2.At the isothermal condition of 27℃, there were exothermal peaks at 40.812∼44.724 seconds from the start of irradiation. The percentage of polymerization by released heat was 19.12∼49.44% after 18 seconds, 37.23∼82.07% after 36 seconds, and 93.57∼98.53% after 54 seconds from the start of irradiation. 3.From FT-IR, the degree of polymerization of composite resin was 17.17∼60.91% and that of compomer was 7.74∼40.82%, and the degree of final conversion was influenced by the kinds of restorative materials and the irradiation energy(p<0.01). 4.As the irradiation energy was increased from 1,500 mJ/㎠ to 16,000 mJ/㎠, the degree of conversion was increased also. However, the degree of final conversion was no significant differences with the irradiation energy of 24,000 mJ/㎠ and 16,000 mJ/㎠(p>0.05). The wavenumber of absorbance peak of aromatic double bond was similar regardless of the restorative materials, and appeared around 1606.67∼1610.67 cm-1. The absorbance peak of aliphatic double bond appeared around 1636.64∼1638.52 cm-1 from FT-IR.

석산폐석을 활용한 쇄석골재의 물리적 및 화학적 특성 연구

송기범,김경수 大田産業大學校 1996 한밭대학교 논문집 Vol.13 No.0201

골재의 수요는 매년 크게 중가하고 있으나 천연 골재는 점차 고갈되어 가고 있다. 따라서 기존석산들에서 발생되는 폐석자원을 쇄석골재로 활용하면 일거양득의 효과가 있다. 본 연구는 국내에서 가장 대규모 석산단지인 포천 및 익산지역 기존석산에서 발생되는 석산폐석에 대한 물리적, 화학적 및 광물학적 시험·분석을 실시하여, 쇄석골재로서 석산폐석의 특성규명과 활용가능성을 평가하였다. 연구결과, 포천과 익산지역에서 발생되는 석산폐석을 쇄석골재로 활용할 수 있을것으로 판단되었으며, 폐석자원을 쇄석골재로 활용함으로써 골재의 수요에 보충하고, 산림 및 자연경관의 훼손과 환경오염을 야기할 수 있는 석산골재 신규개발 의 억제에도 효과가 기대된다. The natural aggregate have been enormously consumed and eventually scarce due to the gradual growth of urbanization during the last several decades, a replacement of the conventional material being necessary. Such a replacement can be achieved by the utilization of quarry wastes. The annual quantity of the wastes produced in the Pocheon and Igsan areas, which are known as the biggest quarry sites in this country, was estimated and their quality was investigated as well, The quarry wastes collected from both of the areas were tested for their physical properties, chemistry and mineralogy in order to appraise their utilization potential as crushed aggregate. Based on all the test results, the wastes of the quarries in the Pocheon and Igsan areas May be utilized as crushed aggregate. It is expected that the quarry wastes will control the future development of quarry only for the production of aggregate which may give rise to the damage and pollution of natural environment.

영지버섯(Ganoderma Iucidum) 생장점으로부터 분리한 단백다당류의 면역활성

정경수,김상범,정수현 충남대학교 약학대학 의약품개발연구소 1997 藥學論文集 Vol.13 No.-

Two protein-polysaccharide fractions. GLA and GLB, respectively, were prepared from the pileus of the fully grown carpophores and the tips of the growing carpophores of Ganoderma lucidum. At a dose of 100 ㎎/㎏/day ip. GLA and GLB inhibited the growth of sarcoma 180 solid tumor in ICR mice by 56.3% and 81.8%, respectively. In a flow cytometric (FCM) analysis. GLA and GLB enhanced the formation of lymphoblasts of BALB.c splenic leukocytes at a concentration of 100㎍/㎖, by 38.3% and 61.3%, respectively. When ip injected into ICR mice. GLB exerted anti-leukopernic effect against cyclopospamide (75 ㎎/㎏/day ip) in that the leukocyte counts of the peripheral blood of the normal and the cycloposphamide-treated mice, respectively, was (11.1±3.8)×10 exp(3) and (4.0±1.8)×10 exp(3), while the GLB-cyclophosphamide treated mice showed a leukocyte count of (10.8±5.1)×10 exp(3) cells/㎕. These results suggest that GLB is a promising candidate for an effective cancer immunotherapeutic agent.

광장공포증 유무에 따른 공황장애 환자의 특이적인 대처전략 비교

류경환,김정범 대한생물치료정신의학회 1999 생물치료정신의학 Vol.5 No.2

요 약본 연구는 공황장애 환자에서 공황발작을 조절하기 위해 흔히 사용되는 대처전략을 조사하고, 광장공포증의 유무에 따른 대처전략 사용을 비교하여 공황장애와 광장공포증 사이의 일부 관련성을 밝히고자 한다. DSM-Ⅳ 진단기준에 의거하여 공황장애로 진단된 환자 51명을 대상으로 하였으며, 그 중 광장공포증을 가진 환자(광장공포증군)는 30명(58.8%), 광장공포증을 가지지 않은 환자(비광장공포증군)는 21명(41.2%)이었다. 대처전략 조사를 위해 Cox 등(1992)의 연구에서 사용된 14가지 대처전략 항목들을 이용하였고, 각 대처전략의 사용 유무와 그 효과 정도를 평가하였다. 연구 결과 공황발작동안 대처전략 중 '안심시킴'이 가장 흔히 사용되었으며, '약물복용'이 가장 효과가 있었다. 두 군 모두 '약물복용'이 가장 효과가 있었으나, 광장공포증군에서 '가까운 사람을 찾음'의 대처전략 사용이 비광장공포증군과 비교하여 유의하게 효과가 있었다. 전체 대상환자 중 여성군보다 남성군에서 '약물복용'이 유의하게 효과가 있었다. 본 연구의 결과는 공황장애 환자들이 향후 공황발작동안 '약물복용'에 더 의존할 가능성이 높고, 광장공포증을 가진 환자에서 '가까운 사람을 찾음'을 안전신호로 사용하는 대처전략이 광장공포증적 회피의 발생이나 유지에 하나의 중요한 역할을 할 수 있음을 시사한다. 향후 치료적인 면에서 바람직한 대처전략의 사용과 안전신호 사용으로 초래되는 부정적인 결과에 대한 환자 교육이 필요할 것으로 생각된다.Objectives : To clarify some aspects of the relationship between panic disorder and agoraphobia, this study compared specific coping strategies employed to control panic attacks between patients with panic disorder without agoraphobia(PD) and with panic disorder with agoraphobia(PDA).Methods : Fifty one patients were diagnosed with PD(N=21) and PDA(N=30) by DSM-IV. All subjects were given the 14 specific coping strategies administered in the Cox et al's study(1992), then indicated which of 14 coping strategies they used together with the efficacy of a particular strategy of reducing the severity of a panic attack where 1 = totally ineffectively and 5 = completely effectively.Results : The most commonly used coping strategies for all subjects was 'reassure self over soon' and the highest efficacy ratings was for 'take medication'. Subjects with PDA reported significantly higher efficacy ratings than did subjects with PA for 'be with friend', but there were no significant differences in both groups for other coping strategies. Male subjects reported significantly higher efficacy ratings than did female subjects for 'take medication'. Female subjects reported significantly higher efficacy ratings than did male subjects for 'smoke cigarette'.Conclusions : The results suggest that the coping strategy 'be with friend' may play an important role in the development and maintenance of agoraphobic avoidance among panic patients. Treatment program for panic patients should include emphasizing the use of positive coping strategies during panic attacks and increasing awareness of the negative consequences resulting from using safety signals.

강진만 해역의 다모류 군집의 종조성 및 계절변동

윤성민,김춘철,서원일,황선영,이성훈,한경호,이우범 國立麗水大學校 環境問題硏究所 2004 環境硏究論文集 Vol.6 No.-

To detemine seasonal fluctuations in abundance and speices composition, polychaete samples were collected by Van Veen grab form March to November 2003 in Kangjin Bay. A total of 25,119.3 ind./㎡ polycaete were sampled and identified into 48 species. 25 families and 10 orders. Of the 10 orders. Phllodocida, Spionida, Eunicida and Sabellida accounted for approximately 79.9% of the polychaete fauna in this area. Phyllodocida, Nereidae, Nephtyidae ans Spionidae were dominant representing every moment 4 species. Of 48 species identified Neanthes japonica, Prionospio pinnate, Cirratulus cirratus and Palola siciliensis for 54.0% individuals collected. Season succession of dominant species was evident in study area: Neanthes japonica, Prionospio pinnata. Cirratulus cirratus. Nephtys oligobranchia in March, Neanthes japonica, Lumbrineris japonica. Cirratulus cirratus, Prionospio pinnata in May, Ncanthes japonica, Cirratulus cirratus, Lumbrineris nippnica. Lumbrineris japonica in August and Neanthes japonica, Prionospio pinnata, Laonice cirrata, Palola siciliensis in November, respectively. The diversity index was the highest value in March(H'=3.0059) and the lowest value in May(H'=2.7305). The eveness index was the highest in August(J=0.9184) and lowest in May(J=0.8524). The dominance index was the highest in May(D=0.3843) and the lowest in March(D=0.2275).

全羅南北道 水域의 管束植物相과 그 分布

吉奉燮,尹敬源,盧範鎭,金昌煥 圓光大學校 基礎自然科學硏究所 1989 基礎科學硏究誌 Vol.8 No.3

全甫北 地域에 있는 6個 江과 고 流域에서 水生植物, 濕生植物과 水邊植物을 조사하였더니 水生植物은 57種類, 濕生植物은 74種類, 水邊植勃 123種類로 나타났다. 이들을 다시 分類하면 56科 145屬 190種 1亞種 27變種 1品種 總 219種類가 된다. 水生植物 中에는 抽水植物이 52.6%', 浮葉植物 21.7%, 沈水植物 19.3%, 浮水植物이 7%를 차지하고 있었다. 이들 中 頻度가 비교적 높은 식물은 검정말, 개구리밥, 노랑어리연꽃이었고 全般的으로 볼 때 優占襪은 검정말이었으나調査地點別로 差異가 있었다. 生物量(g/m^2)은 마름 2,935,5, 물잔디 1.050, 검정말 877.7, 물닭개비 847.1, 애기가래 806.9의 순으로 生量平均値를 기록했고, 乾量(g/m^2)으로는 마름225.4, 물잔디 90.3, 검정말 86.6, 대가래 78.6이었다. 調査地域 中에서 保護할 價値가 있는 곳은 耽津江이고, 植物은 여러곳에 나는 것으로 자라풀,노랑어리연꽃, 순채를 들 수 있다. To investigate the rearing environment of aquatic vascular plants and thechange of its spontaneity, field work was carried out in 6 rivers and theirdrainage basins located in Cholla-nam-but-do area. The flora was composed of 56 families, 145 genera, 190 species, 1 subspecies,27 varieties and 1 forms or 219 taxa; 57 species of hydrophytes, 74 of hygrophytes and 123 of waterside plants, Among hydrophytes emerged plant (E,52.6%), floating-leaved plant (FL,21.7%), submerged plant (S,19.3%) and freefloating planktonic plant (FP, 7%) were occupied, respectively. Dominant species was Hydrilla verlicillaia, but differed according to investigated. A significant place obseryed was Tamjin river and species to beprotected were Hydrocharis dubia, Nympkoides pellata and Braenia schreberi.

1. 06㎛ Nd : YAG Laser를 이용한 요추간판탈출증의 경피적 수핵감압술

이흥선,변박장,최순관,신원한,권귀향,이인수,이경석,김범태 순천향대학교 1994 논문집 Vol.17 No.4

The 1.06㎛ Neodymium-YAG Laser(800 micron hemisherical) is revealed a useful tool for percutaneous laser disc decompression(PLDD). Especially PLDD would be applied to the management of lumbar disc herniation with minimal intervention. Total irradiated energy to one level of disc space was bout 1000 to 2000 joules. We performed this method at 10 levels, in 7 cases of patients, consisting of 4 males and 3 females with an average age of 37.7 years. Five cases showed excellent and good results, were observed. In two cases, open surgery was required because of unsatisfactory results. The best results were obtained in the protrusion type of disc herniation. We have summarized some advantages of PLDD: a) safe and simple procedure in an outpatient setting, b) avoiding general anesthesia, late spinal instability, c) possibility of repetitive use.

초기 당뇨병성 신증과 동반된 Erythropoietin 결핍성 빈혈에 대한 합성 조혈호르몬의 치료효과

김대중,김수경,김형진,김유미,윤용석,안철우,차봉수,송영득,임승길,김경래,이현철,허갑범 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.25 No.5

연구배경:본 연구자 등은 과거에 진행성 당뇨병성 신증 이전 단계에서 원인미상의 빈혈을 동반한 환자들을 관찰할 수 있었으며, 빈형에 대한 Epo의 반응성 감소, 즉 Epo의 상대적 결핍이 빈혈의 원인이 될 수 있음을 보고한 방 있다. 이에 본 연구의 목적은 초기 당뇨병성 신증과 동반된 Epo 결핍성 빈혈에서 rHuEpo의 치료효과를 보고자 하였다. 방법:총 29명의 Epo 결핍성 빈혈을 가진 당뇨병 환자를 Epo 투여군(남:여=8:12, 평균나이 52.9±12.4세)로 무작위 배정하였다. Epo 투여군에게는 rHuEpo(Epokine,㈜제일제당)을 하루 4,000단위씩 주 3회 피하주사하였고 최대 8주간 사용하였다. 추적기간 동안 혈색소치가 2g/dL 이상 상승하거나 혈색소치가 남자 14g/dL, 여자 13g/dL 이상으로 상승한 경우를 반응군으로 하였고 나머지를 비반응군으로 하였다. 결과: Epo 투여군과 대조군 사이의 임상적 및 생화학적 특성의 차이는 없었다. 반응군(n=14)은 비반응군과 대조군에 비해 치료 8주째 의미있는 혈색소치의 상승을 보였다(각각 13.6±1.0, 10.1±1.5, 11.2±1.2g/dL, p<0.01). 반응군의 rHuEpo의 평균투여기간은 4.9±2.3주였다. Epo 투여군중 반응군과 비반응군 사이의 성별, 연령, 당뇨병의 유병기간, 혈청 크레아티닌치, 24시간 소변내 알부민농도, 당뇨병성 미세혈관합병증의 유무, 그리고 혈청 Epo 농도의 차이는 관찰되지 않았다. 그러나 반응군은 비반응군에 비해 혈청 ferritin(각각 240.3±108.4, 25.8±3.0㎍/L, p<0.05)과 transferrin saturation(각각 32.7±7.9, 21.2±5.3%, p<0.05)이 의미있게 높은 소견을 보였다. 결론:이상의 결과로 초기 당뇨병성 신증과 동반된 Epo 결핍성 빈혈에서 rHuEpo의 투여를 통해 빈혈을 교정할 수 있었으며, rHuEpo에 대한 반응의 정도는 당뇨병과 관련된 임상적 특성보다는 기능성 철결핍 정도와 관련되어 있다고 생각된다. Background : We have previously reported that reduced erythropoietin (Epo) responsiveness to anemia could explain the anemia in diabetic patients before advanced diabetic nephropathy. Thus, the aim of this randomized prospective study is to investigate the therapeutic effect of recombinant human erythropoietin (rHuEpo) on anemia with Epo deficiency in early diabetic nephropathy. Methods : Twenty-nine diabetic patients with the normocytic normochromic anemia of Epo deficiency were randomized into Epo-treatment group (n=20, M:F=8:12, mean age=52.9±9.2) and control group (n=9, M:F=4:5, mean age=53.6±12.4). Twenty patients of Epo-treatment group were treated with rHuEpo (Epokine® (CheiUedang Co.) 4,000 unit/ day SC., 3 times/ week) for 8 weeks. The Epotreatment group were divided into the responder or non-responder. Patients with increments in Hemoglobin (Hb) during the follow-up duration was above 2g/ dL, or with the final Hb was above 14g/ dL in men or 13g/ dL in women were decided the responder. In order to analyze factors affecting the therapeutic effects of rHuEpo, the clinical and biochemical characteristics were compared between the responder and non-responder group. Results : There was no difference in the clinical and biochemical characteristics between the Epo-treatment and the control group at randomization. The responder group (n=14) had significant increments in Hb, compared to the non-responder group (n=6) or the control group (13.6±1.0 vs. 10.1±1.5 vs 11.2±1.2g/ dL, p<0.001, respectively). The treatment duration of rHuEpo in the responder group was 4.9±2.3 weeks. Among the Epo-treatment group, there was no differences between the responder and the non-responder group in sex, age, duration of diabetes, serum creatinine level, 24 hour urinary album in excretion rates, HbA_1c, frequency or severity of microangiopathy, and serum Epo level. However, the responder group had higher serum ferritin (240.3±108.4 vs 25.8±3.0 ㎍/L, p<0.05) and transferin saturation level (32.7±7.9 vs 21.2±5.3 %, p<0.05). Conclusion : These results concluded that the administration of rHuEpo could be useful in treating anemia with Epo deficiency in early diabetic nephropathy and that the degree of iron storage and functional iron deficiency might affect the therapeutic effects of rHuEpo on this type of anemia (J Kor Diabetes Asso 25:364~373, 2001).

Hypothalamic Angptl4/Fiaf Is a Novel Regulator of Food Intake and Body Weight

Kim, Hyun-Kyong,Youn, Byung-Soo,Shin, Mi-Seon,Namkoong, Churl,Park, Kyeong Han,Baik, Ja Hyun,Kim, Jae Bum,Park, Joong-Yeol,Lee, Ki-up,Kim, Young-Bum,Kim, Min-Seon American Diabetes Association 2010 Diabetes Vol.59 No.11

<P><B>OBJECTIVE</B></P><P>The angiopoietin-like protein 4 (Angptl4)/fasting-induced adipose factor (Fiaf) is known as a regulator of peripheral lipid and glucose metabolism. In the present study, we investigated the physiological role of Angptl4 in central regulation of body weight homeostasis.</P><P><B>RESEARCH DESIGN AND METHODS</B></P><P>Hypothalamic Angptl4 expression levels were measured using immunoblot assay during feeding manipulation or after administration of leptin, insulin, and nutrients. The effects of Angptl4 on food intake, body weight, and energy expenditure were determined following intracerebroventricular (ICV) administration of Angptl4 in C57BL/6 mice. Food intake, energy metabolism, and feeding responses to leptin, insulin, and nutrients were compared between Angptl4-null mice and their wild littermates. Finally, the relationship of hypothalamic AMP-activated protein kinase (AMPK) and Angptl4 was studied.</P><P><B>RESULTS</B></P><P>Hypothalamic Angptl4 expression levels were increased upon food intake or administration of leptin, insulin, and nutrients. Furthermore, central administration of Angptl4 suppressed food intake and body weight gain but enhanced energy expenditure. These effects were mediated via suppression of hypothalamic AMPK activities. Consistently, Angptl4-null mice displayed increased body weight and hypothalamic AMPK activity but reduced energy expenditure. Food intake following a fast was significantly greater in Angptl4-null mice, which was normalized by centrally administered Angptl4. Moreover, anorectic responses to leptin, insulin, and glucose were diminished in Angptl4-null mice. In contrast, Angptl4-null mice were resistant to diet-induced obesity, indicating obesity-promoting effects of Angptl4 under the condition of fat-enriched diet.</P><P><B>CONCLUSIONS</B></P><P>We have demonstrated that hypothalamic Angptl4 is regulated by physiological appetite regulators and mediates their anorexigenic effects via inhibition of hypothalamic AMPK activity. Therefore, Angptl4 appears to have an important role in central regulation of energy metabolism.</P>