5종의 치주질환균에 대한 프로폴리스의 항균효과

이은지,김소민,박소민,박복리,홍성익,김현정 순천향대학교 기초과학연구소 2022 순천향자연과학연구 논문집 Vol.28 No.1,2

Since the oral cavity is directly connected to the outside, it is an important site for microbial invasion, and since it is an environment that is nutritionally and physiologically easy to multiply bacteria, many bacteria always form bacteriological flora in the oral cavity. And propolis is a natural treatment used in many health food stores in various forms for local treatment. This study analyzes the changes in the activity of five oral microorganisms before and after consumption of propolis for various age groups to find out how propolis, which shows the efficacy of anti-inflammatory, antibacterial, and immune sensitization, affects oral microorganisms. Five types of bacteria possessed by periodontal disease patients were specified and digitized targeting the public person, and then before and after propolis intake was compared, and the levels of periodontal disease bacteria in the intake group and the non-consumption group were compared. As a result of comparing the patterns before ingestion (0 week) and after ingestion (3 week) in the oral bacteria analysis, it was confirmed that the bacteria decreased overall except for the five populations in the propolis intake group. In contrast, in the comparative group that did not intake propolis, the bacteria of five periodontal disease increased except for the four populations. What can be seen from this result is that propolis is effective in reducing bacteria in five types of periodontal disease. In order to use propolis as a food for preventing periodontal disease and to get a better effect, further research is needed to find out the mechanism of antibacterial action in propolis compounds.

Exogenous JH and ecdysteroid applications alter initiation of polydnaviral replication in an endoparasitoid wasp, Cotesia plutellae (Braconidae: Hymenoptera)

( Bok Ri Park ),( Yong Gyun Kim ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.6

Polydnaviruses are a group of double-stranded DNA viruses and are symbiotically associated with some ichneumonoid wasps. As proviruses, the replication of polydnaviruses occurs in the female reproductive organ at the pupal stage. This study analyzed the effects of two developmental hormones, juvenile hormone (JH) and ecdysteroid, on the viral replication of Cotesia plutellae bracovirus (CpBV). All 23 CpBV segments identified contained a conserved excision/rejoining site (``AGCTTT``) from their proviral segments. Using quantitative real-time PCR based on this excision/rejoining site marker, initiation of CpBV replication was determined to have occurred on day 4 on the pupal stage. Pyriproxyfen, a JH agonist, significantly inhibited adult emergence of C. plutellae, whereas RH5992, an ecdysteroid agonist, had no inhibitory effect. Although RH5992 had no effect dose on adult development, it significantly accelerated viral replication. The results of immunoblotting assays against viral coat proteins support the effects of the hormone agonists on viral replication. [BMB reports 2011; 44(6): 393-398]

RNA interference of two ovary transcripts of an endoparasitoid wasp, Cotesia plutellae, suppresses replication of its symbiotic polydnavirus

Bok-ri Park,Yonggyun Kim 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05

Polydnaviruses (PDVs) are a group of insect double stranded DNA viruses and symbiotically associated with host endoparasitoid wasps. Their segmented genome is located in host chromosome(s) in a proviral form. Viral replication is initiated at the ovary during late pupal stages. Little is known about the factors involved in the viral replication. This study analyzed the ovarian transcripts of an endoparasitoid wasp, Cotesia plutellae, by 454 pyrosequencing and subsequent gene annotation. Out of 2,226 contigs and 12,457 singletons, 50 transcripts categorized in DNA replication, coat proteins, and viral origins were selected as putative viral replication factors. The selected genes were analyzed in their expressions according to host wasp development. Quantitative real-time RT-PCRs showed that some of the selected genes were expressed during the viral replication at late pupal stage. Using RNA interference, five putative genes were tested in their implication in the viral replication by analyzing viral DNA amplification, structure of ovarian calyx, and parasitism. RNA interference of contig#1004 (broad complex) or contig#174 (a viral DNA polymerase gene) significantly inhibited DNA amplification without any impairment of viral formation, and subsequently resulted in significant reduction in the wasp parasitism. This study reports that two wasp genes (or not encapsidated viral genes) are implicated in the viral DNA amplification and viral coat protein production during the polydnaviral replication.

Effect of Hot Water Treatments for Disinfection on Meloidogyne spp. and Heat Damage of Allium hookeri

Bok-ri Park,Bo-Ram Kim,YuJeong Shin,NohYoul Heo,Donghun Cho 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.10

Imported Allium hookeri is sometimes infected by some quarantine nematodes like Meloidogyne spp., Pratylenchus spp. Hot water treatment(HWT) is reported as the most effective treatment method for disinfection of nematodes. The primary goal of this research was to determine the temperature tolerance of Allium hookeri and lethal temperature of Meloidogyne spp., preferably in the range of 5~30minutes at 48~53℃. Second stage juveniles of Meloidogyne spp. were successfully eliminated in hot water bath treated at 48℃ within 1 minute. Egg hatching was suppressed completely at 48℃ more than 26 minutes. No evidence of growth damage was observed on plants treated with HWT even at 48℃ for 30 minutes and 49℃ for 10 minutes. Therefore, the optimum range of HWT is recommended at 48℃ for 30 minutes and 49℃ for 10 minutes on Allium hookeri infected root-knot(Meloidogyne spp.) nematode.

New Records of Host Plant for Radopholus similis Isolated from Agathis dammara

Na-Ri Lee,Dong Woo Kim,Bo-ram Kim,Bok-ri Park,Jae yong Chun 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.04

The aim of the study is to report Radopholus similis detected from Agathis dammara in Thailand as new host plant. Existing host of R. similis was known as coffee, pepper, sugarcane and banana etc. This nematode in this study was observed morphologic character using Carl Zeiss Axioimager M2 and Axiovision Rel. 4.8 program. The result shows that a and b of the female was distorted toward maximum value of original description of species, and b′, c and stylet length was distorted toward minimum value of original description of species. This nematode was morphologically distinguished from originally detected R. similis, but measured value was similar in range of original description of species. For more information, molecular assay was also confirmed the R. similis with 98% homology with the sequence of the internal transcribed spacer region (ITS) of ribosomal DNA. We provided PCR-amplified ITS nucleotide sequence.

A Modified Method of Genomic DNA Extraction of Meloidogyne spp. (Tylenchida: Meloidogynidae) for Molecular Assay

Bo-Ram Kim,Bok-ri Park,Yu Jeong Shin,Noh Youl Heo,Jae-Yong Chun 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.10

Recently, researches of molecular biology for the identification of root-knot nematode (RKN) species have been reported in plant quarantine. In this study, applicable and reproducible method to extract high quality genomic DNA from single nematode (Meloidogyne spp.) was developed. Also, the modified method was verified by DNA manipulation techniques such as PCR amplification and cloning. Single juvenile was floated in a drop of water and digested with proteinase K for 24 h. After that, DNA was extracted by using distilled water as extraction buffer. PCR amplification was carried out with universal primers spanning the internal transcribed spacer (ITS) region to distinguish species. When using the existing DNA detection method, quantification results showed that 42.86% of the deposited DNA was extracted. Whereas the modified DNA extraction method was increased to 100%. When PCR products test the direct sequencing using the ITS rDNA primers, it was also identified as M. javanica, M. incognita, and M. hispanica. Based on the studies conducted, the application of this modified method would be useful and efficient on plant parasitic nematode molecular assay.

New Record of Host Plant for Radopholus similis Isolated from Staurogyne sp.

Bo-Ram Kim,Na Ri Lee,Bok-ri Park,Jae yong Chun 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.04

Radopholus similis belong to the plant parasitic nematode family, Pratylenchidae and was recognized that injure the plant. R. similis was generally identified from bananas and plantain in the tropics. However, the occurrence of infection of R. similis on new host plant was confirmed recently. The aim of this study was to report the determination of new host plant for R. similis on Staurogyne sp. The species identification was evaluated the morphological and molecular characteristics of nine R. similis. Molecular assay was designed from the internal transcribed spacer region (ITS) of ribosomal DNA to distinguish species. Nematodes from Staurogyne sp. showed almost similar morphological and morphometric characteristics in general R. similis, but some variation in tail shape was confirmed. Also, male was not detectable. Molecular assay showed a high level (97%) of similarity in the TW81-AB28 and 18S-28S sequences (ITS region) with corresponding NCBI sequence. The quarantine should be more intensely to detect the prohibited nematode.

Taxonomy of $Ganoderma$ $lucidum$ from Korea Based on rDNA and Partial ${\beta}$-Tubulin Gene Sequence Analysis

Park, Young-Jin,Kwon, O-Chul,Son, Eun-Suk,Yoon, Dae-Eun,Han, Woo-Ri-Ja-Rang,Yoo, Young-Bok,Lee, Chang-Soo The Korean Society of Mycology 2012 Mycobiology Vol.40 No.1

In the present study, a phylogenetic analysis was undertaken based on the internal transcribed spacer (ITS) rDNA and partial ${\beta}$-tubulin gene sequence of the $Ganoderma$ species. The size of the ITS rDNA regions from different $Ganoderma$ species varied from 625 to 673 bp, and those of the partial ${\beta}$-tubulin gene sequence were 419 bp. Based on the results, a phylogenetic tree was prepared which revealed that Korean $Ganoderma$ $lucidum$ strains belong in a single group along with a $G.$ $lucidum$ strain from Bangladesh.

Estimation of Genetic Parameters for Serum Clinical-Chemical Traits in Korean Native Chickens

Park, Hee-Bok,Seo, Dong-Won,Choi, Nu-Ri,Choi, Jun-Seung,Heo, Kang-Nyeong,Kang, Bo-Seok,Jo, Cheorun,Lee, Jun-Heon The Korean Society of Poultry Science 2012 韓國家禽學會誌 Vol.39 No.4

Clinical-chemical traits are commonly used biomarkers to examine the health status of individuals. There is an appreciable range of normal variation in most clinical-chemical traits and the determining factors of this variation have been relatively uninvestigated in chickens. The aim of this study was to estimate the genetic parameters (i.e., heritability, genetic correlation) for 8 clinical-chemical traits (glucose, total protein, creatinine, high-density lipoprotein cholesterol, total cholesterol, glutamic oxaloacetic transaminase, glutamic pyruvic transaminase and amylase) in an $F_1$ intercross established by purebred breeding among the 5 lines of Korean native chickens. Phenotypic data were collected from approximately 600 $F_1$ animals. The genetic parameters for the clinical-chemical traits estimated by a mixed animal model using the restricted maximum likelihood method were presented. Estimated heritabilities ranged from 8.9% (glucose) to 39.6% (high-density lipoprotein cholesterol). Interestingly, both the sign and the size of the genetic and phenotypic correlations were largely different between the same several pair of clinical-chemical traits. The findings in this study will provide useful information to address issues in both quantitative trait locus study and genetic management in Korean native chickens.

Anticoagulant and Antiplatelet Activities of Artemisia princeps Pampanini and Its Bioactive Components

Ri Ryu,Un Ju Jung,Hye-jin Kim,Wonhwa Lee,Jong-Sup Bae,Yong Bok Park,Myung-Sook Choi 한국식품영양과학회 2013 Preventive Nutrition and Food Science Vol.18 No.3

Artemisia princeps Pampanini (AP) has been used as a traditional medicine in Korea, China and Japan and reported to exhibit various beneficial biological effects including anti-inflammatory, antioxidant, anti-atherogenic and lipid lowering activities; however, its antiplatelet and anticoagulant properties have not been studied. In the present study, we evaluated the effects of an ethanol extract of Artemisia princeps Pampanini (EAP) and its major flavonoids, eupatilin and jaceosidin, on platelet aggregation and coagulation. To determine the antiplatelet activity, arachidonic acid (AA)-, collagen-and ADP (adenosine diphosphate)-induced platelet aggregation were examined along with serotonin and thromboxane A₂ (TXA₂) generation in vitro. The anticoagulant activity was determined by monitoring the activated partial thromboplastin time (aPTT) and prothrombin time (PT) in vitro. The data showed that EAP and its major flavonoids, eupatilin and jaceosidin, significantly reduced AA-induced platelet aggregation and the generation of serotonin and TXA₂, although no significant change in platelet aggregation induced by collagen and ADP was observed. Moreover, EAP significantly prolonged the PT and aPTT. The PT and/or aPTT were significantly increased in the presence of eupatilin and jaceosidin. Thus, these results suggest that EAP may have the potential to prevent or improve thrombosis by inhibiting platelet activation and blood coagulation.