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RISS 인기검색어
- 검색키워드
- 저자 : Raymer, Paul (검색결과 5 건)
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Development of Seashore Paspalum Turfgrass with Herbicide Resistance
Kim, Kyung-Moon,Song, In-Ja,Lee, Hyo-Yeon,Raymer, Paul,Kim, Beom-Seok,Kim, Wook The Korean Society of Crop Science 2009 Korean journal of crop science Vol.54 No.4
Seashore Paspalum (Paspalum vaginatum Swartz) is a warm season grass and indigenous to tropical and subtropical regions of coastal areas worldwide. The species is used as feed for cattle and horses and has been very successful for golf courses worldwide. One of the most outstanding characteristics of seashore paspalum is its tolerance to saline soils compared to other warm season turfgrasses. The development of new seashore paspalum cultivars with improved traits could be facilitated through the application of biotechnological strategies. The purpose of this study was to product for herbicide resistant seashore paspalum using Arobacterium-mediated transformation and this study is the first report on transformation and herbicideresistant transgenic plants in seashore paspalum. Embryogenic calli were induced from the seeded variety of pseashore paspalum. Embryogenic calli were transformed with Agrobacterium tumefaciens strain EHA105 carrying the binary vector pCAMBIA3301 with two genes encoding gusA and bar. Transformed calli and plants were selected on medium containing 3 mg/l PPT. PCR detected the presence of the gusA and bar gene, indicating both genes are integrated into the genome of seashore paspalum. A chlorophenol red assay was used to confirm that the bar gene was expressed. By application of herbicide BASTA, the herbicide resistance in the transgenic seashore paspalum plants was confirmed.
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Effect of Potassium Nitrate Priming on Seed Germination of Seashore Paspalum
Shim, Sang In,Moon, Jun-Cheol,Jang, Cheol Seong,Raymer, Paul,Kim, Wook American Society for Horticultural Science 2008 HortScience Vol.43 No.7
<P>Germination of seashore paspalum (<I>Paspalum vaginatum</I> Swartz) is a critical factor influencing seedling establishment when seeded directly in fields. In this research, pregermination priming with osmotic solution was used to improve the germination percentage of seashore paspalum. The goal of this study was to develop techniques that improve the germination of a seashore paspalum cultivar (SeaSpray). Seeds were subjected to priming in petri dishes with solutions of KNO3 at 25 °C for 24, 48, and 72 h in growth chambers. Germination percentage differed by priming duration as well as concentration of priming media. Based on the germination percentage 14 days after imbibition, the most promising priming condition was the treatment with 0.2% or 0.5% KNO3 for 72 h at a constant temperature of 30 °C and 0.2% or 0.5% KNO3 for 48 to 72 h at alternating temperature of 25/35 °C. Both concentrations showed reasonable germination percentage greater than 85% at alternating temperature condition. Priming with KNO3 solution for 48 to 72 h improved not only germination percentage, but also uniformity. The increased duration of priming with KNO3 was positively correlated with an improved germination percentage. The effect of increasing concentration was the most apparent at a constant temperature (30 °C) regime with the treatment of 0.2% KNO3 priming. Germination percentage was increased from 34.3% to 68.0% 2 weeks after imbibition (WAI) as the priming duration was increased from 24 to 72 h. Priming with KNO3 for 3 days also had a modest effect on germination percentage (greater than 74.7%) at 1 WAI. Therefore, priming with 0.2% or 0.5% solution of KNO3 for 72 h is a recommended method that can be practically applied for increasing germination of paspalum under an alternating temperature (25/35 °C) condition. The regression analysis between odds of germination percentage and germination time showed that priming treatment increased internal activities during the second stage of seed germination.</P>
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Kang, Yoonshik,Cho, Kiyoung,Noh, Jaewoo,Vitullo, Dashiell L. P.,Leary, Cody,Raymer, M. G. The Optical Society 2010 Optics express Vol.18 No.2
<P>We propose and provide experimental evidence in support of a theory for the remote preparation of a complex spatial state of a single photon. An entangled two-photon source was obtained by spontaneous parametric down-conversion, and a double slit was placed in the path of the signal photon as a scattering object. The signal photon was detected after proper spatial filtering so that the idler photon was prepared in the corresponding single-photon state. By using a two-photon coincidence measurement, we obtained the Radon transform, at several longitudinal distances, of the single-photon Wigner distribution function modified by the double slit. The experimental results are consistent with the idler photon being in a pure state. An inverse Radon transformation can, in principle, be applied to the measured data to reconstruct the modified single-photon Wigner function, which is a complete representation of the amplitude and phase structure of the scattering object.</P>
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Development of Seashore Paspalum Turfgrass with Herbicide Resistance
Kyung-Moon Kim,In Ja Song,Hyo-Yeon Lee,Paul Raymer,Beom Seok Kim,Wook Kim 韓國作物學會 2009 Korean journal of crop science Vol.54 No.4
Seashore Paspalum (Paspalum vaginatum Swartz) is a warm season grass and indigenous to tropical and subtropical regions of coastal areas worldwide. The species is used as feed for cattle and horses and has been very successful for golf courses worldwide. One of the most outstanding characteristics of seashore paspalum is its tolerance to saline soils compared to other warm season turfgrasses. The development of new seashore paspalum cultivars with improved traits could be facilitated through the application of biotechnological strategies. The purpose of this study was to product for herbicide resistant seashore paspalum using Arobacterium-mediated transformation and this study is the first report on transformation and herbicideresistant transgenic plants in seashore paspalum. Embryogenic calli were induced from the seeded variety of pseashore paspalum. Embryogenic calli were transformed with Agrobacterium tumefaciens strain EHA105 carrying the binary vector pCAMBIA3301 with two genes encoding gusA and bar. Transformed calli and plants were selected on medium containing 3 mg/l PPT. PCR detected the presence of the gusA and bar gene, indicating both genes are integrated into the genome of seashore paspalum. A chlorophenol red assay was used to confirm that the bar gene was expressed. By application of herbicide BASTA, the herbicide resistance in the transgenic seashore paspalum plants was confirmed.
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