Platycodon grandiflorum root-derived saponins attenuate atopic dermatitis-like skin lesions via suppression of NF-kB and STAT1 and activation of Nrf2/ARE-mediated heme oxygenase-1

( Jae Ho Choi ),( Sun Woo Jin ),( Eun Hee Han ),( Bong Hwan Park ),( Hyung Gyun Kim ),( Tilak Khanal ),( Yong Pil Hwang ),( Minh Truong Do ),( Hyun Sun Lee ),( Young Chul Chung ),( Hee Suk Kim ),( Tae 영남대학교 약품개발연구소 2014 영남대학교 약품개발연구소 연구업적집 Vol.24 No.0

Purpose: The consequences of precipitously rising allergic skin inflammation rates worldwide have accelerated the risk of atopic dermatitis (AD). Natural product-based agents with good efficacy and low risk of side effects offer promising prevention and treatment strategies for inflammation-related diseases. We have already reported that Platycodon grandiflorum root-derived saponins (Changkil saponins, CKS) have many pharmacological effects, including anti-inflammatory and anti-allergic effects, but its influence on AD remains unclear. Therefore, we evaluated the inhibitory effect of CKS, mainly platycodin D, on AD-like skin symptoms in mice and the possible mechanisms in cells. Methods: Mice were sensitized and challenged with 2,4-dinitrochlorobenzene (DNCB). Four weeks after challenge, mice were treated with oral administration of CKS for 4 weeks. In addition, cells were used to evaluate the effect of CKS, mainly platycodin D, on the TARC expression regulated mechanism. Results: CKS attenuated DNCB-induced dermatitis severity, serum levels of IgE and TARC, and mRNA expression of TARC, TNF-α, IFN-γ, IL-4, IL-5, and IL-13 in mice. Histopathological examination showed reduced thickness of the epidermis/dermis and dermal infiltration of inflammatory cells and mast cells in the ears. Moreover, CKS and platycodin D inhibited TNF-α/IFN-γ-induced TARC expression through the suppression of NF-κB and STAT1 and induction of Nrf2/ARE-mediated hemeoxygenase-1 (HO-1) expression in cells. Conclusion: We suggest that CKS and platycodin D inhibited the development of AD-like skin symptoms by regulating cytokine mediators and may be an effective alternative therapy for AD-like skin symptoms.ⓒ2014 Elsevler Gmbh. All rights reserved.

요추부 척추협착증 수술환자에서 극돌기간 연성고정물 사용군과 비사용군의 치료결과 비교

김재도,장재호 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.1

Background : To compare of the clinical and radiologic results between microscopic decompression(MD) and MD with Device for Intervertebral Assisted Motion(DIAM) in lumbar canal stenosis. Materials and Methods : The retrospective evaluation was performed after surgery of stenosis. MD with DIAM was performed in 17 cases(Group A) and MD in 20 cases(Group B). In radiologic evaluation, checked the preoperative and postoperative disc height, intervertebral foraminal dimension, lumbar lordotic angle and instability by Panjabi. In clinical evaluation, preoperative and postoperative pain was checked by VAS. Disability was checked by RMQ(Roland Morris Questionnaire) and sf-36(PF). Results : In radiologic results, disc height changed from 11mm to 13mm in Group A(11mm to 11.8mm in Group B)(p=0.669). Intervertebral foraminal dimension was changed from 20.6mm to 22.3mm in Group A(19.5mm to 20.5mm in Group B)(p=0.536). Lumbar lordotic angle was changed from 15.40 to 14.20 in Group A(14.80 to 19.50 in Group B)(p=0.033). Instability score was improved from 0.9 to 0.2 in Group A(1 to 0.2 in Group B)(p=0.642). VAS score improved in group A from 10 to 2(9 to 2 in Group B)(p=0.612). On sf-36(PF), the values changed from 34/100 to 58/100 in Group A(30/100 to 58/100 in Group B)(p=0.535). Conclusion : Using dynamic interspinous stabilizer(DIAM) appears to be a useful and effective methods in surgery of lumbar canal stenosis with better radiologic results than MD only, but lumbar lordotic angle was decreased, slightly

糖, 아미노酸 및 無機窒素化合物이 人蔘褐變促進에 미치는 影響

都在浩,金相達,吳勳一,洪淳根 한국응용생명화학회 1982 Applied Biological Chemistry (Appl Biol Chem) Vol.25 No.3

紅蔘褐變의 適合한 促進方法을 究明하기 위하여 人蔘에 糖, amino酸 및 無機窒素化合物을 處理한 結果 糖類中에서는 maltose와 glucose가 가장 强한 褐變促進作用을 나타내었다. amino酸은 basic amino acid인 lysine, histidine, arginine이 가장 크게 促進했으며 糖과 amino酸 混合處理에서는 glucose와 glutamic acid 退合處理區가 가장 크게 促進되었다. Glucose 濃度別 處理時의 褐變促進效果는 glucose濃度가 增加함에 따라 褐變이 促進되었으며 無機窒素化合物中에서는 urea>(NH₄)₂)HCO₃>COONH₄등의 順으로 褐變이 促進되었다. In order to find out pertinent methods for the acceleration of browing during ginseng processing, various treatments were made or fresh ginseng (Panax Ginseng C.A. Meyer) with sugars, amino acids and inorganic nitrogenous compounds and the extent of browning was measured. Among sugars tested, maltose resulted in the greatest acceleration of browning followed in decreasing order by glucose and lactose, whereas pentoses, fructose, sucrose and raffinose had negligible effect. A marked browning occurred in ginseng treated with basic amino acids, while the extent of browning was not greatly increased when ginseng was treated with aliphatic amino acids, hydroxy amino acids, or acidic amino acids used in the experiment. Among treatments with sugar-amino acid mixture, a mixture of glucose with glutamic acid gave the greatest acceleration. The brown color intensity gradually increased with an increase in glucose concentration for up to 0.5M. inorganic nitrogenous compounds enhanced the browning in general, and the effect varied greatly with the different compounds.

만성두통환자에게 적용한 비약물적 치료가 통증행동에 미치는 영향

최도영,임사비나,차남현,김건식,이재동,김수영,이윤호,이두익 EAST-WEST MEDICAL RESEARCH INSTITUTE KYUNG HEE UNI 2005 東西醫學硏究所 論文集 Vol.2005 No.-

Objectives : This study is a random-controlled post-design for confirmation of headache degree, quality of life, and satisfaction to therapeutical effect on chronic headache adults after management of non-medicinal treatment (acupuncture therapy and stellate ganglion block therapy). Methods : 51 clinical experiment participants were gathered and through a questionnaire patients who experienced headache for more than 4 hours a day and more than 15 days per month were qualified as chronic headache patients. The qualified patients were classified in to two groups, acupuncture group (AT group, n=28) and stellate ganglion block group (SGB group, n=23). Treatment was applied 2 times a weeks for 4 weeks. The effects of both groups were analyzed using VAS scores, BPI (Brief Pain Inventory) and the satisfaction degree to the therapy. Results : 1. The recognized score of the headache of AT group and SGB group was reduced indicating the degree of the headache was released. 2. The recognition at damage to the quality of life was reduced post therapy of AT group and SGB group, in which general activity, mood, enjoyment of life, personal relationship, and sleeping showed significant improvement of life quality. 3. The satisfaction degree to the therapy showed lower score than expectation to it in AT group and SGB group, however, it was not significant. Conclusions : The results showed that the four-week non-medicinal treatment (AT and SGB) in chronic headache patients was effective for reducing headache and releasing its damage in daily life, however, no difference in superiority was found. Therefore, non-medicinal treatment (AT therapy and SGB) could be utilized in chronic headache patients.

만성 호중구성 백혈병 1례

기장도,박치영,류보열,김태종,모성환,곽재정,문철호,박근홍,박유환,정춘해 朝鮮大學校 附設 醫學硏究所 1996 The Medical Journal of Chosun University Vol.21 No.1

Chronic neutrophilic leukemia is a very rare myeloproliferative disorder which is characterized by severe sustained mature neutrophilic leukocytosis in peripheral blood (PB), hepatosplenomegaly, elevated leukocyte alkaline phosphatase, serum uric acid. serun viatmin B 12 and the bleeding tendency despite normal platelet count and coagulation time, absence of philadelphia chromosome, and the absence of fever or underlying infection or disease sufficient to mimic a leukemoid reaction. We have experienced a 34-years-old male patient with atypical chronic neutrophilic leukemia. On admission, the leukocyte count was 56,860/uL. severe neutrophic leukocytosis with 76% neutrophils and rare immature forms on PB smear. Many neutrophils had toxic granules, and vacuoloes. Leukocyte alkaline phosphatase score was decreased. The cytogenetic study showed Philadelphia chromosome negative with normal karyotype.

核分裂 飛跡에 依한 黑曜石의 年代測定

姜榮浩,金道聖,高在杰 慶北大學校 物理化學硏究所 1984 硏究論文集 Vol.5 No.-

Charged particles create their tracks in most dielectric solids. These tracks can be viewed under optical microscope after proper chemical etching. Tracks of uranium fission fragments in obsidians also can be viewed after etching by HF solution and used for age dating. In this paper, dating formular and experimental procedures for fission track dating are introduced. And spontaneous and induced fission tracks by thermal neutrons are counted under optical microscope (magnification of 400x). Measured fission track ages of obsidian samples, OM No. 147 and HM No. 40 are 8.86 × 10 exp(5)y and 7.67 × 10 exp(5)y, respectively.

다발성 골수종으로 이행한 단일형질세포종 1예

정재용,모성환,기장도,문철호,김창욱,전용준,박유환,정춘해,오윤경 조선의대 1995 The Medical Journal of Chosun University Vol.20 No.2

시나리오 역할 게임을 통한 신속 시스템 설계/검증 방법 : 패키지 응용 시스템을 대상으로 for Package Based System Implementation

예도경,이용재,강두호,최열현 한국경영과학회 1998 한국경영과학회 학술대회논문집 Vol.- No.1

Recently, many enterprises wish to preoccupy competitive positions in the market by motivating themselves towards CALS(Commerce At Light Speed), and try hard to achieve system implementation with the use of new concept-based backbone systems such as ERP(Enterprise Resource Planning) or ERP(Enterprise Resource Planning). However, unfortunately there is an inevitable discrepancy during implementation period between package vendors and customers due to lack of clear methodology and misunderstanding about system. In some cases, consensus on partially verified system design invokes customers’ continuous change demands and often it tends to result in unnecessary project delays. PDM application system implementation project of the company L has been studied here to solve these sorts of problems. The major yields of this work are achievement of a willing approval from customers and rapid system design under a time-limited and conceptually defined project scope, and finally, through fully verified system design, we were able to reach successful system implementation.

回轉圓板의 應力擴大係數에 관한 硏究 : Stress Freezing Method of Photoelastic Experiment

최선호,이충섭,권재도,황재석 嶺南大學校 工業技術硏究所 1986 연구보고 Vol.14 No.2

It is well known fact that the stress distributions in the vicinity of crack tip and fracture problems are governed by stress intensity factor which is presented as a function of the crack shape, boundary condition and loading mode etc. In. the determination of the stress intensity factor, there are many theoritical and experimental methods. Among them the stress freezing method of photoelastic experiment has been considered to be one of the most accurate experimental methods which have been used for stress analysis. In this paper, this method was applied to the rotating disk with inner arbitrary crack. The results of photoelastic experiment for disk with various straight cracks, radial and others which are oblique to the radical direction were as follows. In case of radial crack, in comparison with the results of photoelastic experiment obtained by stress freezing method and the theoritical solutions of Blauel, stress freezing method is fully available for analysis of uniform dynamic status.

Banana 栽植樣式이 生育과 收量에 미치는 影響

吳現道,朴才昊,金龍湖 濟州大學校 亞熱帶農業硏究所 1987 亞熱帶農業硏究 Vol.4 No.-

本 試驗은 1985年 3월 부터 1986年 3月에 걸쳐 栽植樣式이 生育과 收量에 미치는 影響을 究明하기 위하여 濟州大學校 亞熱帶農業硏究所 plastic film house에서 遂行되었다. 그 結果를 要約하면 다음과 같다. 바나나의 草長, 葉數, 葉幅, 葉長, 果掌數, 果房重은 栽植樣式에 따라 影響을 받지 않았으나 假莖長, 假莖直徑, 抽穗期, 成熟期는 有意性이 인정되어 正方形, 三角形, 長方形, 栽植樣式 順으로 假莖長과 假莖直徑이 增加하였고 抽穗期와 成熟期도 短縮되었다. 果掌當 果指數와 果掌重은 栽植樣式에 따라 1段에서 差異가 있었고 長方形, 三角形 栽植樣式에서 2段에서는 현저하게 감소하였으나 3段 이후부터는 緩慢하게 감소되는 傾向을 나타내었다. 果掌當 果指長과 果指直徑은 1段에 比해 2段에서 增加하였는데 長方形, 正方形, 栽植樣式에서 1段∼2段 差가 컸으며 果指直徑의 變化는 栽植樣式 및 果掌間에 따라서 差異가 크게 나타났다. 正方形, 三角形 栽植樣式에서 1段에 比해 2段 果指重이 增加하였으나 3段 이후부터는 감소되는 경향을 나타내고 栽植樣式 및 果掌間에 따라 差異가 컸다. This experiment was carried out to elucidate the influence of spatial arrangement at planting on the growth and yield in bananas (Musa cavendish Lambert 'Dwarf Cavendish') from March, 1985 to March, 1986 in the plastic film house at Research Institute for Subtropical Agriculture, Cheju National University, Cheju, Korea. The results obtained are summarized as follows. Plant height, no. of leaves, leaf width, leaf length, no. of hands, and bunch weight, were little affected by planting pattern. However, pseudostem length and diameter were greater and date of emergence and maturity were earlier in the order of regular, triangular, rectangular planting. The number of fingers/hand and hand weight were significantly affected by planting pattern in 1st hands and decreased remarkably in 2nd hand of the rectangular and triangular planting, but tended to be decreased slowly from 3rd hand. Finger length and diameter/hand were greater in 2nd hand than in 1st hand and more significant in the difference of 1-2 hands in rectangular and regular planting than that of triangular planting, finger diameter/hand were afected remarkably by planting pattern or hands. Finger weight/hand were increased in 2nd hand against 1st at regular and triangular planting, but tended to be decreased from 3rd hand and affected by planting pattern or hands.