고분자 키토산 처리가 딸기 과실의 품질과 부패에 미치는 영향

황용수,구자형 충남대학교 농업과학연구소 2004 농업과학연구 Vol.31 No.2

This study was aimed to determine the effect of high molecular weight chitosan treatment on the decay incidence and fruit quality in strawberries. Preharvest spray of chitosan (0.2%) effectively reduced the incidence of grey mould and increased fruit firmness. Chitosan application was also effective on remaining of surface gloss. This is probably due to coating effect. Physiological disorders were not found in treatment of 4 sprays. Internal quality such as soluble solid content and acidity seemed not to be affected by chitosan application. The effect of chitosan on keeping freshness was clear in fruit without physical damage at harvest, but muck less effective in injured fruit. Postharvest application of chitosan did not affect fruit quality. Above results indicated that chitosan is an alternative to keep strawberry quality through delaying decay and keeping firmness.

애착 이론과 그 영향에 대한 고찰

황혜자,조수진 東亞大學校 2005 東亞論叢 Vol.42 No.-

This study examines attachment theory, and increases understanding of it by comparing with psychoanalysis theory. We have gone over previous research results and looked at what influence attachment has throughout an individual's life-span. Attachment theory is a theory that conceptualizes people's tendency to feel attachment to certain people, and explains various emotional trauma and personality disorder which is accompanied by unwanted separation or loss from an attachment object. Attachment theory thinks of attachment as a person's first drive in comparison to psychoanalysis theory, and gave much information such as various patterns of attachment, conditions of secure attachment, development of attachment throughout a person's lifespan, by focusing on the relationship between a mother and her child. Attachment has great affect on children's emotional development, social development and cognitive development. Also research shows that attachment has constancy throughout a person's lifespan, and influences self-identity, self-esteem, cognition of oneself and others, social relationships, independence and separation-individuation, adjustment, career development and career exploration, depression, anxiety, eating disorder, and coping with stress. Therefore attachment does not pertain only to children, but is a fundamental drive of a human being, and applying attachment theory to counseling has an important significance.

적절한 용량 이하의 경구용 향응고제 사용 중 발생한 뇌출혈 환자의 임상 양상

조수진,황흥곤,나찬영,이영탁,최현배,조태구 世宗醫學硏究所 2000 世宗醫學 Vol.17 No.2

녹차의 주성분인 에피갈로카테킨 갈레이트의 정맥주사후 흰쥐에서의 약물속도론 연구

임재수,황성주,지웅길,김동출 충남대학교 약학대학 의약품개발연구소 2000 藥學論文集 Vol.16 No.-

The pharmcacokinetic study of epigallocatechin gallate was performed following iv bolus administration in Sprague-Dawley rats. The values of systemic clearance (CL) were 68±5 and 27±1 ml/min/kg following iv bolus administration of 1 mg and 5 mg EGCG, respectively. The values of volume of distribution at steady state were 380 and 835 ml/kg body weight after iv bolus administration 1mg and 5mg EGCG, respectively. The decrease in the value of CL and the increase of Vss as a function of dose suggest saturable pharmacokinetics of EGCG in rats.

트래드밀 운동프로그램 적용이 성인비만자의 심폐기능과 혈청지질에 미치는 영향

최건식,정영자,김남익,황수관 대한스포츠의학회 1994 대한스포츠의학회지 Vol.12 No.2

The purpose of this study was to investigate the effect of treadmill exercise programs on cardiopulmonary functions and serum lipids in adult obesity men. The subjects consisted of 33 obese adults between 30 and 70 years old and all of them had no other complications. Subjects participated in treadmill exercise programs for 12 weeks according to the exercise prescriptions. They started to exercise for 20 minutes per day, six times a week at 50% of maximum O_2 consumption (V˙O_2max), and later exercised for 50 minutes at 80% of V˙O_2max, which was the maximum exercise intensity of the program (Exercise intensity has been increased gradually). The changes of body composition, serum lipids, and cardiopulmonary functions in the subjects before and after the treadmill exercise program have been measured. The results are as follows. 1. Body weight, percent body fat, and fat body weight were reduced(p<0.05, p<0.01), and body fluid were increased after exercise programs(p<0.01). The group below 50 years old and above 25% of body fat showed more decrease in body weight and percent body fat, and more increase than that above 50 years old below 25% of body fat. 2. Resting heart rate, systolic and diastolic blood pressure were significantly reduced after exercise programs(p<0.05, p<0.01), but these parameters decreased approximately same for the groups of ages and percent body fat. 3. Vital capacity, forced expiratory volume for 1 second percent and maximal voluntary ventilation were increased after exercise programs. The group below 50 years old and above 25% of body fat showed more increase than that above 50 years old and below 25% of body fat. 4. Total cholesterol, LDL cholesterol, total cholesterol/HDL cholesterol rate and triglycerides were reduced (p<.05, p<0.01), and HDL cholesterol levles were increased after exercise programs. The group below 50 years old and above 25% of body fat showed more decrease in total cholesterol, LDL cholesterol, total cholesterol/HDL cholesterol rate and triglycerides, and more increase than that above 50 years old and below 25% of body fat. 5. Heart rate increase during exercise were reduced after exercise programs. The group below 50 years old and above 25% of body fat showed more decrease than that above 50 years old and below 25% of body fat. 6. Blood pressure increase during exercise were reduced after exercise programs. The group above 25% of body fat showed more decrease than that below 25% of body fat. 7. Oxygen uptake of during exercise were increased after exercise programs. 8. Maximal oxygen uptake were significantly increased after exercise programs (p<0.01). 9. The regression equations between heart rate, systolic blood pressure, and oxygen uptake were obtained. These correlation coefficients of the post-programs showed higher that those of pre-programs. In this study, the treadmill exercise programs suitable for the obese was developed and applying this programs weight, percent of body fat, body fat weight, and serum lipids levels ere reduced. Thus cardiopulmonary functions was improved. The group below 50 years old and above 25% of body fat showed more decrease than that above 50 years old and below 25% of body fat. It is to be noted that both systolic blood pressure and oxygen uptake can be estimated by heart rate only using the regression equations.

Urapidil, Labetalol의 투여가 기관내 삽관시 심혈관계에 미치는 영향

신용섭,윤석화,손수창,이원형,이정은,황원재,김만수,김영주,김혜자,최세진 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2

We have examined the comparative efficacy of small doses of intravenous urapidil and labetalol in blunting hemodynamic response to endotracheal intubation and surgical incision in 30 patients without cardiovascular diseases. After intravenous urapidil 0.2 mg/kg or labetalol 0.2 mg/kg anesthesia was induced with thiopental 5mg/kg. Endotracheal intubation was facilitated by vecuronium 0.15 mg/kg with priming principle and anesthesia was maintained with enflurane and nitrous oxide in oxygen. Systolic, diastolic and mean arterial pressure and heart rate were measured before administration of the drugs, 5 minute after administration, just prior to endotracheal intubation and 1, 3, 5, 10 minute after intubatin. Also the peak blood pressures and heart rate within 10 minutes after surgical incision were measured. Endotracheal intubation and surgical stimulation were associated with significant increases in blood pressures and heart rate in both urapidil and labetalol group. Comparison of the changes in systolic, diastolic, and mean artrial pressures and heart rate between urapidil and labetalol group showed no significant difference except peak systolic pressure after surgical incision. It is concluded that the pressor response to endotracheal intubation and surgical stimulation are not influenced significantly by urapidil 0.2 mg/kg or labetalol 0.2 mg/kg. However, urapidil and labetalol preloading may be similarly effective in the blunting of the increases in blood pressures with larger doses of the durgs during anesthetic induction.

Effects of the water of yellow soil, Ji-Jang-Soo on cell viability and cytokines production in immune cells

Jeong, Hyun-Ja,Hwang, Gab-Soo,Myung, No-Il,Lee, Joon-Ho,Lee, Ju-Young,Um, Jae-Young,Kim, Hyung-Min,Hong, Seung-Heon Kyung Hee Oriental Medicine Research Center 2006 Oriental pharmacy and experimental medicine Vol.6 No.1

Ji-Jang-Soo (JJS) is known to have a detoxification effect. However, it is still unclear how JJS has these effects in experimental models. In this study, we investigated the effect of JJS on the viability of cells and production of cytokines in human T-cell line, MOLT-4 cells, and human mast cell line, HMC-1 cells. The MOLT-4 cells were cultured for 24 h in the presence or absence of JJS. As the result, JJS (1/100 dilution) significantly increased the cell viability about 78% (P < 0.05) and also increased the interleukin (IL)-2, and interferon $(IFN)-{\gamma}$ production compared with media control at 24 h. But had no effect on IL-4 production. Hypoxia mimic compound, desferroxamine (DFX) decreased the immune cell viability. Cell viability decreased by DFX was increased by JJS. In conclusion, these data indicate that JJS may have an immune-enhancing effect.

Stachybotrysin, an Osteoclast Differentiation Inhibitor from the Marine-Derived Fungus <i>Stachybotrys</i> sp. KCB13F013

Kim, Jong Won,Ko, Sung-Kyun,Kim, Hye-Min,Kim, Gun-Hee,Son, Sangkeun,Kim, Gil Soo,Hwang, Gwi Ja,Jeon, Eun Soo,Shin, Kee-Sun,Ryoo, In-Ja,Hong, Young-Soo,Oh, Hyuncheol,Lee, Kyung Ho,Soung, Nak-Kyun,Hashi American Chemical Society and American Society of 2016 Journal of natural products Vol.79 No.10

<P>Two new phenylspirodrimane derivatives, stachybotrysin (1) and stachybotrylactone B (2), were isolated from the cultures of the marine derived fungus Stachybotrys sp. KCB13F013. The structures were determined by analyzing the spectroscopic data (1D and 2D NMR and MS) and chemical transformation, including the modified Mosher's method and single-crystal X-ray structure analysis. Compound 1 exhibited an inhibitory effect on osteoclast differentiation in bone marrow macrophage cells via suppressing the RANKL-induced activation of p-ERK, p-JNK, p-p38, c-Fos, and NFATc1.</P>

Original Articles : Comparison of the bioavailability of nano- and micro-sized copper oxide particles in copper-deficient mice

( Ja Ktyung Seol ),( Jae Hwang Jeong ),( Sang Yoon Nam ),( Young Won Yun ),( Jong Soo Kim ),( Beom Jun Lee ) 한국예방수의학회(구 한국수의공중보건학회) 2015 예방수의학회지 Vol.39 No.1

Abstract: Copper is an essential micronutrient whose deficiency is often seen to occur in humans. Although many biomedicalstudies have focused on the use of nanoparticles, the nutritional effects of nano-sized copper oxide particles are not wellknown. This aim of this study was to investigate the nutritional bioavailability of nano- and micro-sized copper oxide (CuO)particles in copper-deficient (CuD) mice. Copper deficiency was induced in mice by feeding a CuD diet (0.93 mg Cu/kg diet)for 7 weeks. After the induction of copper deficiency, nano- or micro-sized copper oxide particles were administered orallyat two different doses (0.8 and 4.0 mg CuO/kg body weight) to mice in the following groups: (1) normal control (NC), (2)CuD, (3) low dose micro-sized CuO, (4) high dose micro-sized CuO, (5) low dose nano-sized CuO, and (6) high dosenano-sized CuO. The hepatic copper concentration in the CuD group was significantly lower than that in the NC group. Compared to the NC group, the CuD group exhibited lower serum ceruloplasmin (CP) activity and CP level. Thecopper/zinc-superoxide dismutase activity in the CuD group was significantly lower than that in the NC group. Treatment withnano- or micro-sized copper oxide particles for 2 weeks restored the hepatic copper levels and serum CP activities to valuessimilar to those observed in the NC group. The CP levels and copper/zinc-superoxide dismutase activities in all the copperoxide treatment groups also recovered to normal values after 3 weeks of copper oxide treatment. These results show thatoral administration of either nano- or micro-sized copper oxide particles for 2?3 weeks restored the normal condition inpreviously CuD mice.

Glucosamine exerts a neuroprotective effect via suppression of inflammation in rat brain ischemia/reperfusion injury

Hwang, So-Young,Shin, Joo-Hyun,Hwang, Ji-Sun,Kim, Song-Yi,Shin, Jin-A,Oh, Eok-Soo,Oh, Seikwan,Kim, Jung-Bin,Lee, Ja-Kyung,Han, Inn-Oc Wiley Subscription Services, Inc., A Wiley Company 2010 Glia Vol.58 No.15

<P>We investigated the neuroprotective effect of glucosamine (GlcN) in a rat middle cerebral artery occlusion model. At the highest dose used, intraperitoneal GlcN reduced infarct volume to 14.3% ± 7.4% that of untreated controls and afforded a reduction in motor impairment and neurological deficits. Neuroprotective effects were not reproduced by other amine sugars or acetylated-GlcN, and GlcN suppressed postischemic microglial activation. Moreover, GlcN suppressed lipopolysaccharide (LPS)-induced upregulation of proinflammatory mediators both in vivo and in culture systems using microglial or macrophage cells. The anti-inflammatory effects of GlcN were mainly attributable to its ability to inhibit nuclear factor kappaB (NF-κB) activation. GlcN inhibited LPS-induced nuclear translocation and DNA binding of p65 to both NF-κB consensus sequence and NF-κB binding sequence of inducible nitric oxide synthase promoter. In addition, we found that GlcN strongly repressed p65 transactivation in BV2 cells using Gal4-p65 chimeras system. P65 displayed increased O-GlcNAcylation in response to LPS; this effect was also reversed by GlcN. The LPS-induced increase in p65 O-GlcNAcylation was paralleled by an increase in interaction with O-GlcNAc transferase, which was reversed by GlcN. Finally, our results suggest that GlcN or its derivatives may serve as novel neuroprotective or anti-inflammatory agents. © 2010 Wiley-Liss, Inc.</P>