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Lee, Young Ju,Lee, Doyeon,Shin, So Min,Lee, Jin Sun,Chun, Hyun Soo,Quan, Fu-Shi,Shin, Jae Ho,Lee, Gi-Ja ELSEVIER SCIENCE B.V.; AMSTERDAM 2017 JOURNAL OF FUNCTIONAL FOODS Vol.33 No.-
<P><B>Abstract</B></P> <P>In this study, we investigated the cardioprotective effects of fermented garlic extract (FGE) against ischemia-reperfusion (IR) injury in H9c2 cells and myocardium. FGE used in this study is rich in nitrite (1.42mg/ml of FGE) and reduced glutathione (GSH, 3.23mg/ml of FGE). We compared the effectiveness of FGE against HR–induced oxidative stress with that of nitrite, GSH, and a mixture of nitrite and GSH (nitrite+GSH). In addition, we utilized fluorinated xerogel-derived oxygen microsensors to measure the changes in oxygen level (<I>p</I>O<SUB>2</SUB>) of the myocardium during myocardial IR by FGE. As a result, the <I>p</I>O<SUB>2</SUB> in the FGE group restored to pre-ischemic level (75.1±36.9%) at reperfusion period; however, that of the control group did not increase throughout reperfusion period (2.0±1.7%, <I>p</I> <0.05). Therefore, pre-treatment with FGE might be helpful as a therapeutic strategy for preventing H9c2 cells and myocardium from IR injury.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We investigated the cardioprotective effect of FGE against myocardial IR injury. </LI> <LI> FGE was rich in natural nitrite (1.422mg/ml) and glutathione (3.23mg/ml). </LI> <LI> Pre-treatment with FGE could protect H9c2 cells from HR-induced injury. </LI> <LI> pre-treatment with FGE could promote reoxygenation in post-ischemic myocardiaum. </LI> <LI> FGE might be useful in preventing myocardial IR injury. </LI> </UL> </P>
Yu, Namhee,Yong, Seunghui,Kim, Hong Kwan,Choi, Yoon‐,La,Jung, Yeonjoo,Kim, Doyeon,Seo, Jihae,Lee, Ye Eun,Baek, Daehyun,Lee, Jinseon,Lee, Seungjae,Lee, Jong Eun,Kim, Jaesang,Kim, Jhingook,Lee, Sa John Wiley and Sons Inc. 2019 MOLECULAR ONCOLOGY Vol.13 No.6
<P>The roles of miRNAs in lung cancer have not yet been explored systematically at the genome scale despite their important regulatory functions. Here, we report an integrative analysis of miRNA and mRNA sequencing data for matched tumor–normal samples from 109 Korean female patients with non‐small‐cell lung adenocarcinoma (LUAD). We produced miRNA sequencing (miRNA‐Seq) and RNA‐Seq data for 48 patients and RNA‐Seq data for 61 additional patients. Subsequent differential expression analysis with stringent criteria yielded 44 miRNAs and 2322 genes. Integrative gene set analysis of the differentially expressed miRNAs and genes using miRNA–target information revealed several regulatory processes related to the cell cycle that were targeted by tumor suppressor miRNAs (TSmiR). We performed colony formation assays in A549 and NCI‐H460 cell lines to test the tumor‐suppressive activity of downregulated miRNAs in cancer and identified 7 novel TSmiRs (miR‐144‐5p, miR‐218‐1‐3p, miR‐223‐3p, miR‐27a‐5p, miR‐30a‐3p, miR‐30c‐2‐3p, miR‐338‐5p). Two miRNAs, miR‐30a‐3p and miR‐30c‐2‐3p, showed differential survival characteristics in the Tumor Cancer Genome Atlas (TCGA) LUAD patient cohort indicating their prognostic value. Finally, we identified a network cluster of miRNAs and target genes that could be responsible for cell cycle regulation. Our study not only provides a dataset of miRNA as well as mRNA sequencing from the matched tumor–normal samples, but also reports several novel TSmiRs that could potentially be developed into prognostic biomarkers or therapeutic RNA drugs.</P>
The Effects of Preoperative Aspirin on Coronary Artery Bypass Surgery: a Systematic Meta-Analysis
Doyeon Hwang,Joo Myung Lee,Tae-Min Rhee,Young-Chan Kim,Jiesuck Park,Jonghanne Park,Chul Ahn,Young Bin Song,Joo-Yong Hahn,Ki-Bong Kim,Young-Tak Lee,Bon-Kwon Koo 대한심장학회 2019 Korean Circulation Journal Vol.49 No.6
Background and ObjectivesAspirin plays an important role in the maintenance of graft patency and the prevention of thrombotic event after coronary artery bypass graft surgery (CABG). However, the use of preoperative aspirin is still under debate due to the risk of bleeding. MethodsFrom PubMed, EMBASE, and Cochrane Central Register of Controlled Trials, data were extracted by 2 independent reviewers. Meta-analysis using random effect model was performed. ResultsWe performed a systemic meta-analysis of 17 studies (12 randomized controlled studies and 5 non-randomized registries) which compared clinical outcomes of 9,101 patients who underwent CABG with or without preoperative aspirin administration. Preoperative aspirin increased chest tube drainage (weighted mean difference 177.4 mL, 95% confidence interval [CI], 41.3–313.4; p=0.011). However, the risk of re-operation for bleeding was not different between the preoperative aspirin group and the control group (3.2% vs. 2.4%; odds ratio [OR], 1.23; 95% CI, 0.94–1.60; p=0.102). There was no difference in the rates of all-cause mortality (1.6% vs. 1.5%; OR, 0.98; 95% CI, 0.64–1.49; p=0.920) and myocardial infarction (MI) (8.7% vs. 10.4%; OR, 0.83; 95% CI, 0.66–1.04; p=0.102) between patients with and without preoperative aspirin administration. ConclusionsAlthough aspirin increased the amount of chest tube drainage, it was not associated with increased risk of re-operation for bleeding. In addition, the risks of early postoperative all-cause mortality and MI were not reduced by using preoperative aspirin.
Lee, Geumbee,Kim, Daeil,Kim, Doyeon,Oh, Sooyeoun,Yun, Junyeong,Kim, Jihyun,Lee, Sang-Soo,Ha, Jeong Sook The Royal Society of Chemistry 2015 ENERGY AND ENVIRONMENTAL SCIENCE Vol.8 No.6
<P>In this study, we report the fabrication of a stretchable and patchable array of micro-supercapacitors (MSCs) using a gel-type electrolyte of poly(methyl methacrylate)–propylene carbonate–lithium perchlorate. As electrodes, a layer-by-layer-assembled thin film of multi-walled carbon nanotubes with a top layer of Mn<SUB>3</SUB>O<SUB>4</SUB> nanoparticles was used. The fabricated MSC maintained over 85% of its performance for 2 weeks in ambient air without encapsulation owing to the use of a non-aqueous solvent based gel electrolyte. Dry-transferred MSC arrays on a specially designed stretchable polymer substrate exhibited stable electrochemical performance under various deformations, including bending, twisting, both uniaxial and biaxial stretching up to 50%, and winding around the curved substrate. Furthermore, the encapsulated MSC array with a thin polymer film directly attached to skin maintained its electrochemical performance under repeated body movement, cycles of attachment–detachment, and even in water. This study clearly demonstrates a stretchable and patchable MSC array for practical use as an energy storage device that can be attached to the body for electronic function, even under wet conditions.</P> <P>Graphic Abstract</P><P>We report on air stable planar micro-supercapacitors (MSCs) using a non-aqueous solvent based gel electrolyte and stable performance of encapsulated MSC array under stretching and exposure to water. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c5ee00670h'> </P>
Development of bioinformatics and multi-omics analyses in organoids
Doyeon Ha,JungHo Kong,Donghyo Kim,Kwanghwan Lee,Juhun Lee,Minhyuk Park,Hyunsoo Ahn,Youngchul Oh,Sanguk Kim 생화학분자생물학회 2023 BMB Reports Vol.56 No.1
Pre-clinical models are critical in gaining mechanistic and biologicalinsights into disease progression. Recently, patient-derivedorganoid models have been developed to facilitate our understandingof disease development and to improve the discoveryof therapeutic options by faithfully recapitulating in vivo tissuesor organs. As technological developments of organoid modelsare rapidly growing, computational methods are gaining attentionin organoid researchers to improve the ability to systematicallyanalyze experimental results. In this review, we summarize therecent advances in organoid models to recapitulate human diseasesand computational advancements to analyze experimentalresults from organoids.
권도연(Doyeon Kwon),정창규(Changkyou Jung),박광범(Kwangbeom Park),이철균(Choul-Gyun Lee),이진원(Jinwon Lee) 한국생물공학회 2011 KSBB Journal Vol.26 No.2
The aim of the study was to optimize harvesting method for concentrating microalgae from microalgae mass culture. It is well known that the mass density of microalgae is usually very low and these are small size (5-20 μm) in the culture medium. It is essential that microalgae is harvested and concentrated economically for economical biodiesel production from microalgae. In this study, to determine optimized conditions for microalgae harvesting by chemical flocculation. Flocculation of three algae, Chlorella ellipsoidea, Dunaliella bardawil, and Dunaliella tertiolecta, was performed using various chemical flocculants, such as inorganic flocculants (aluminium sulfate, aluminium potassium sulfate, ferrous sulfate, ferric sulfate, ferric chloride, calcium hydroxide, sodium carbonate, sodium nitrite, and sodium aluminate), organic flocculant (polyacrylamide), and biopolymer flocculants (chitosan and starch). The results indicated that aluminium based inorganic flocculants is suitable for microalgae harvesting such as Chlorella ellipsoidea, Dunaliella bardawil, and Dunaliella tertiolecta. The results also recommended that flocculant doses, agitation speed, agitation time, sedimentation time for economical microalgae harvesting method using chemical flocculants.