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      • Monensin이 反芻動物 第一胃內 微生物과 그 活性에 미치는 影響에 關한 硏究

        李學喆,李垠 영남대학교 자원문제연구소 1983 資源問題硏究 Vol.2 No.-

        The present experiments were conducted to determine the effect of feeding monensin on rumen bacterial flora and protozoal fauna, on bacterial enzyme activity involving amylase and urease, on ruminal pH and volattile fatty acids. Nine Korean native goats averaging 15.6 kg of body weight were randomly alloted to the diets supplemeted with monensin at the level of 0, 30 and 60ppm. Alters 3 weeks adaptation period the animals were fed the experimental diets. Ruminal fluids were collected and examined just before feeding, and 2, 4, 6 and 10 hrs after feeding on days 0, 7, 14, 28, and 42. 1. The total number of ruminal protozoa was markedly decreased with monensin diets from the 7th to 28th day of the experimental period, although it appeared to be recovered gradually towards the end of experiments. However, a significant difference (p<.05) among treatments could be noted in the total number of ruminal protozoal throughout the experimental period. 2. A total of 20 protozoal species were detected throughout the period: 17 species in Ophryoscolacidae, 3 species in Isotrichide. The number of those was decreased by mnonensin diets, so that the protozal composition of monensin groups was simpler than that of the control. 3. The protozoal fluctuation pattern was changed by the monensin treatment, and the fluctuation of each species consisted of the protozoal composition was varied according to not only species, but also treatments. The counts of protozoa of Holctricha order were appeared to be increased by monensin treatments while no significant differences were noted in counts of protozoa of Spirotricha-order except Eudiplodinium species shown slight increase number. 4. In the total number of ruminal bacteria, nonsignificant difference (p>.05) was observed throughout the experimental period. 5. Fourteen different kinds of the ruminal bacteria types were classified by means of morphology and Gram·stain reaction. In the total number of bacterial types, nonsignifi-cant difference (p> .05) was observed among treatments. 6. The bacterial fluctuation pattern was changed by the monensin treatment, and the fluctuation of each bacterial type consisted of the bacterial composistion was varied according to bacterial types and monensin levels. The total numbers of Gram-negative bacteria increased(15.78~16.41%) with monensin treatments, while the numbers of Gram-positive bacteria decreased (69.65~73.09%) throughout the experimental period. 7. Amylase activity in ruminal fluids of the monensin diet tend to be increased fromt he 7th to 27th or 42nd day of the experimental period : although no significant difference(p) .05) in amylase activity was noted among treatments. 8. Urease activity in ruminal fluids of the 30 ppm diet appeared to be increased from the 7th to 28th day of the experiod, the 60 ppm diet tend to be decreased from the 7th to the end of the experiment. However, no significant difference(p> .05) was observed between the 30 ppm and control group, and the 60 ppm group was significant from the other two groups. 9. In the ruminal pH, values nosignificant difference (p> .05) was found among treat-ments throughout the experimental period. 10. The total rumial VFA concentration was found not to be affected by the monensin treatments. 11. The molar portion of acetate and butyrate in rumen fluids were reduced(p <.06) by monensin diets, propionate, however, increased(p <.05) from the 7th to the end of the-experirnental period. The butyrate was significantly decreased at the 60 ppm level. Non-significant difference (p> .05) was observed in isovalerate and valerate. 12. A significant difference(p <.05) in the average ratio acetate to propionate between 60 ppm level and either control or 30 ppm was observed.

      • Salinomycin 飼料添加가 反芻動物 第一胃內 微生物과 그 活性에 미치는 影響에 關한 硏究

        李學喆,李垠 영남대학교 자원문제연구소 1984 資源問題硏究 Vol.3 No.-

        The present experiments were conducted to determine the effect of feeding salinomycin on rumen bacterial flora and protozoal fauna, on bacterial activity involving amylase and urease, on ruminal pH and volatile fatty acids. Eight Korean native goats averaging 19.3kg of body weight were randomly alloted to the diets supplmented with salinomycin at the level of 0 and 30 ppm. After 3 week adaptation period the animals were fed the experimental diet. Ruminal fluids were collected and examined just before feeding. and 4, 8 hours after feeding on days 0, 7, 14. 28 and 42. 1. The total number of ruminal protozoa was markedly decreased with salinomycia diet from the 7th to 14th day, although it was gradually increased after that, it was not recovered at the end of experiment. A significant difference (p<.05) among treatments could be noted in the total number of ruminal protozoa throughout the experimental period. 2. A total of 14 protofoal species were detected throughout the experimental period: 3 species in Isotrichidae, 11 species in Ophryoscolacidae. The number of protozoal species of salinomycin group was lower than that of control group and the number of Holotricha was increased in salinomycin group. 3. The total of ruminal bacteria tended to increase in salinomlrcin group but no significant difference (p>.05) was observed among treatments and the changes in distribution of bacterial type were similar among treatments. 4. The bacterial fluctuation pattern was varied with bacterial types and the total number of Gram-negative bacteria increased with salinomycin diet, while that of Gram-positive bacteria decreased.

      • KCI등재

        시호(Bupleuri Radix)분말이 고콜레스테롤 급여 흰쥐의 체지질구성 및 TBARS량에 미치는 영향

        李俊茂,李垠,崔武永 대한본초학회 2000 大韓本草學會誌 Vol.15 No.1

        Effects of powdered siho(Bupleuri Radix) on lipid composition and TBARS were investigated in rat fed high cholesterol. Plasma total cholesterol showed a tendency to increase in cholesterol group(P<.OS). However, in the cholesterol group, contents of serum total cholesterol showed no significantly different among treatments(P>.OS). Plasma HDL-cholesterol and triglyceride contents showed no significantly different among treatments(P>.OS). Liver total cholesterol showed a tendency to increase in cholesterol group, however these values showed no significantly different among all the treatments(P>.05). Liver triglyceride contents showed no significantly different among all the treatments(P>.05). Contents of plasma and liver TBARS were high (Pc.05) in control group fed high cholesterol only. however these values were lower by powdered siho(Bupleuri Radix) feeding. Glutathione peroxidasc activity showed low in control group fed high cholesterol only(P<.OS), however these values showed a tendency to increase(P<.OS) by powdered ailio(Bup1euri Radix) feeding

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