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고위험산모와 일반산모의 산후 6주간 증상에 대한 전향적 관찰 연구 : 일개 한방병원 산후조리원을 이용한 산모를 중심으로 = A Prospective Observational Study on Symptoms of the High risk group and Normal group used Postpartum Care Center during Six Weeks after Childbirth in Korean Medicine Hospital
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추모특집-고 이강식형을 생각하며
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인지장애 노인들의 인지기능과 영양섭취 상태에 관한 연구
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청소년을 위한 집단따돌림 예방프로그램 개발과 그 효과 = (The) effect and development of a bulldyin prevention programs for youngsters
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퇴원진료정보의 표준항목 설정
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Differences in the one-repetition maximum and load-velocity profile between the flat and arched bench press in competitive powerlifters
- 검색키워드
- 저자 : 페르난데스 체리 팜비드 (검색결과 2 건)
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Molecular cloning, characterization and biological activity of duck interleukin-17F
페르난데스 체리 팜비드 경상대학교 대학원 2015 국내석사
Interleukin-17F (IL-17F) is a proinflammatory cytokine, which plays an important role in gut homeostasis. A full-length duck IL-17F (duIL-17F) cDNA with a 501-bp coding region was isolated from ConA-stimulated duck splenic lymphocytes. The duIL-17F is predicted to encode 166 amino acids, which include a 26-amino acid signal peptide, single N-linked glycosylation site, and six cysteine residues that are conserved in mammalian IL-17 family. The duIL-17F shares 77.5% amino acid sequence identity with chicken IL-17F (chIL-17F), 37-46% with corresponding mammalian homologues and 53.5% with previously described duck IL-17A (duIL-17A). The duIL-17F transcript was preferentially expressed in a wide range of normal tissues; highest level was detected in the liver and moderate levels were found in thymus, bursa, kidney, and intestinal tissues. Expression levels of duIL-17F transcript were slightly up-regulated in ConA- and LPS-activated splenic lymphocytes, but not in poly I:C stimulated cells. The recombinant duIL-17F, like duIL-17A, induced IL-1β, IL-6, and IL-8 in primary duck embryonic fibroblasts (DEFs), suggesting duIL-17F involvement in inflammatory response and thus, can function as a proinflammatory cytokine. Moreover, duIL-17F directly interacts with duIL-17A and forms a heterodimer. In Salmonella-infected tissues, only expression levels of duIL-17A, but not duIL-17F, were significantly up-regulated in the liver and spleen compared with those of uninfected controls. Thus, further analysis of the contributions of IL-17F and IL-17A to other Salmonella spp. and disease models remains to be elucidated to expand our understanding of its biological functions.
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체리팜비드페르난데스 경상대학교 대학원 2018 국내박사
Although IL-17 cytokines play critical roles in host defense and immunity, dysregulated expression of these cytokines is associated with inflammation and autoimmune diseases. Riemerella anatipestifer (R. anatipestifer) is the most important infectious bacterium in the duck industry which has 5-75% mortality, depending on strain virulence. Interestingly, not all avian species are equally susceptible to R. anatipestifer infection. This study reports the first description of mortality rate, bacterial burden, and expression profiles of immune-related genes between ducks and chickens infected with R. anatipestifer. Ducks exhibited increased susceptibility to R. anatipestifer infection compared to chickens, as determined by mortality rate and bacterial burden. Comparative expression analyses of immune-related genes in R. anatipestifer-infected tissues obtained from both species revealed that IL-4 and IFN-γ transcript levels were higher in chickens, whereas TLR4 and IL-17A transcript levels were higher in ducks. Marked increases in expression levels of IL-17A and IL-6, but not TGF-β, were associated with Th17 cell differentiation in duck splenic lymphocytes, but not in chicken splenic lymphocytes stimulated with R. anatipestifer. Moreover, upregulation of IL-6 and IL-17A mRNA expressions, but not TGF-β, was confirmed in the liver and spleen of ducks infected with R. anatipestifer, indicating that IL-17A is strongly associated with R. anatipestifer infection in ducks. Interestingly, in chickens infected with R. anatipestifer serotypes 1 and 7, the Th2-specific cytokine, IL-4 was generally higher in the spleen and liver than the corresponding levels in ducks. This result suggested a possibility that IL-4 expression is involved in the reduction or suppression of IL-17A expression in chickens, and may account for the lower pathogenicity and mounted a protective immunity in chickens compared with ducks. Thus, a full-length duck IL-4 (duIL-4) cDNA encoding 136 amino acids was identified from ConA-activated splenic lymphocytes. The duIL-4 shares approximately 49.3-50% amino acid sequence identity with quail IL-4 (quIL-4) and chicken IL-4 (chIL-4), respectively and 21-29.7% with mammalian and piscine homologues. Low level of duIL-4 mRNA transcript was found in the spleen, and moderate levels were observed in bursa, kidney and thymus, while relatively high level was detected in the lung. The duIL-4 expression profiles were also examined in mitogen-stimulated splenic lymphocytes, as well as in tissues affected by R. anatipestifer. The levels of duIL-4 were generally upregulated in mitogen-activated splenic lymphocytes but lower in the liver and spleen of R. anatipestifer-infected ducks compared to those of infected chickens. Recombinant duIL-4 promoted nitric oxide synthesis in duck splenic macrophages stimulated with R. anatipestifer compared to untreated and stimulated control. duIL-4 treatment downregulated the expression levels of IL-17A and IL-17F transcripts in the splenic lymphocytes stimulated with R. anatipestifer. Moreover, duIL-4 treatment in R. anatipestifer-stimulated splenic lymphocytes suppressed the expression levels of IL-23p19 and IL-12p40 transcripts compared to untreated and stimulated lymphocytes. Conversely, duIL-4 induced the levels of IFN-γ and IL-10 in IL-4-treated and R. anatipestifer-stimulated splenic lymphocytes compared to untreated and stimulated splenic lymphocytes. These data have demonstrated that duIL-4 is an important Th2 cytokine that inhibits inflammatory responses in splenic lymphocytes stimulated with R. anatipestifer. This study demonstrated that proinflammatory cytokines including IL-17A are involved in inflammation during, and regulating susceptibility to, R. anatipestifer infection and suppression of IL-17 production could alleviate the development of the disease. Hence, in this study, the effects of the anti-inflammatory compound berberine (BBR) in duck splenic lymphocytes stimulated with heat-killed R. anatipestifer, and in R. anatipestifer-infected ducks were also investigated. IL-17A, IL-17F and IL-1β transcripts were downregulated, and IFN-γ and IL-10 transcripts were enhanced in berberine-treated stimulated splenic lymphocytes, compared to stimulated untreated splenic lymphocytes. Similarly, IL-17A, IL-17F, IL-6, and IL-1β expressions were significantly reduced, and IFN-γ and IL-10 expressions significantly upregulated, in spleens and livers of R. anatipestifer-infected berberine-treated ducks, compared to infected untreated birds. Moreover, infected and treated birds showed increased survival rates and significantly decreased bacterial burdens compared to infected untreated birds, confirming that inflammatory cytokines are strongly associated with R. anatipestifer infection in ducks.
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