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      • AH-66 Hepatoma 세포의 Histone에 관한 연구

        최종오,이동욱,이희성 중앙대학교 의과대학 의과학연구소 1985 中央醫大誌 Vol.10 No.2

        In attempt to investigate histone fractions of tumor cells, histone fractions HI, H2a, H2b, H3 and H4 were prepared from isolated nuclei of AH-66 hepatoma cells and histone fractions were characterized by amino acid analysis and by polyacrylamide disc gel electrophoresis. The results obtained were as follows; 1, The five histone fractions found in most tissues were also present in the AH-66 hepatoma cell histories. 2. The yield of whole histone was 6.46mg per g of packed cells. 3. The yield of DNA was 14.78mg per g packed cells. Consequently the DNA to histone ratio was 1:0.44. 4. The relative amounts of five fractions, i.e., H1, H2a, H2b, H3 and H4 were 15.64% 34.83%, 21,21%, 11.76% and 16.56%, respectively. The data presented in this report show that there is a considerable variation in the relative amounts of histone fractions as compared to those of calf thymus. 5. The electrophoretic mobility of individual histone fractions gave almost similar patterns to those of corresponding fractions of calf thymus. 6. Amino acid analysis of the individual histone fractions showed that the over-all compositions were similar but not identical to those of corresponding fractions from calf thymus and other tumor cells. 7. Histone fractions HI, H2b. H3 and H4 were found to contain detectable amounts of ε-N-monomethyllysine. Usually the methylated derivatives of histidine have been reported in histone Hi fraction of Sarcoma 180 ascites tumor cells and Walker 256 carcinosarcoma. However, the methylated histidine residue was not found in all histone fractions from AH-66 hepatoma cells. 8. The evidence was presented that AH-66 hepatoma cells contained five major histone fractions which were homologous to corresponding five fractions of calf thymus.

      • 캐비닛(Cabinet)의 새로운 생산기술 개념

        최종오,Choe, Jong-O 한국방위산업진흥회 1988 國防과 技術 Vol.- No.114

        캐비닛은 전장에서 기동성 있는 작업공간을 제시하며, 군의 이에 대한 이용도는 이동정비, 지휘통신, 물자수송 등의 측면에서 날로 증가하고 있다. 다음 내용은 서독의 캐비닛 개선에 관한 내용을 번역한 것으로, 개념.계획.완성의 순서로 서술하였다. 이런 점에서 캐비닛은 모체차량으로부터 분리, 운용될수 있어야 하며 핵 및 고주파로부터 방호될 수 있어야 한다는 개념이다. 또한 모체차량의 적재능력 한계로 가능한 캐비닛 자체는 가벼운 재질과 특수제작공법으로 적절한 견고성을 유지할수 있도록 발전시키고 있으며, 사용목적에 알맞도록 캐비닛 내부를 설계, 장비시키고 있는바, 이러한 개선개발 추세를 관련업체나 연구 종사자들이 이해함으로써 개발후 이용도 측면에서 그 효과를 크게 기대할수 있어야 할 것이다.

      • KCI등재
      • KCI등재

        연구장비 공동활용시설의 이용의도에 영향을 미치는 요인에 관한 연구: 건설·교통 분야 시설 이용자 중심으로

        최종오,김언정 한국유통경영학회 2020 유통경영학회지 Vol.23 No.2

        Purpose: Recently, research and development (R&D) investment has increased, and research facilities have become more important at the science and technology infrastructure level. However, research on strategic investment and Co-utilization of research equipment is insufficient compared to its importance. In order to activate public R&D facilities in research and development, managers of public R&D facilities need to understand which aspects of the specified services are best able to explain the user's intention to use and which technical elements the user considers most important. Research design, data, and methodology: This study analyzed the important factors in service quality and technology acceptance for Co-utilization of national R&D facilities and equipments, and, using those factors, which factors influence the intention of use. A total of 130 questionnaires for users in the construction and transportation sectors were used for the final analysis. For the verification of the hypothesis of this research, a model of a structural equation is used, and a program for analysis uses SPSS 20 and AMOS 18. Results: Tangible quality of services was not significant, both for perceived usefulness and for perceived ease of use. On the other hand, intangible quality of service was a significant result of both perceived usefulness and perceived ease of use. It has been shown that perceived ease of use does not affect perceived usefulness. perceived usefulness and perceived ease of use have been shown to affect use intention. Further, it has been shown that perceived usefulness has a greater influence than perceived ease of use, which is judged to be necessary to emphasize perceived usefulness rather than perceived ease of use in order to increase the availability of Co-utilization. Conclusions: The results of this research were slightly different from those of prior studies that explain existing service quality, TAM and use intention. To efficiently operate the Co-utilization facility, it suggests that policies that enhance the Assurance, reliability, responsiveness and empathy of technology are important, in order to operate co-utilization facility efficiently. At the same time, the government needs to be controlled so that it can provide support and education to technician.

      • KCI등재

        영어 영재 학생의 심화학습 프로그램 개발

        최종오,Choi, Jong-Oh 한국영재학회 2003 영재교육연구 Vol.13 No.3

        영재교육은 개인이 가진 능력을 최대한 계발하여 자아를 실현하고, 국가 사회가 필요로 하는 인재 육성의 육성이라는 점에서 그 중요성이 강조되어 왔다. 영재교육이 활성화되기 위해서는 제도적인 측면과 함께 그들의 능력수준과 학습욕구에 적합한 영재교육 프로그램이 개발되어야 한다. 이 연구에 참여한 언어 영재 학생은 총 60명인데, 이 중에서 예비 연구에 참여한 30명은 연구자가 개발한 심화학습 프로그램과 언어 문제해결력 검사의 양호도를 평가하기 위한 대상이었고, 실험집단 30명은 10명씩 세 집단으로 나누어 각 집단에 한국교육 개발원 교재, 연구자가 개발한 심화학습 프로그램, 국어 교과서 심화학습 프로그램에 배정하여 프로그램간의 유의도를 비교 검증하였다. 결과를 종합하면, 언어 영재학생에게 연구자가 개발한 체계화된 개별화 심화학습 프로그램을 제공하면 언어 창의적 문제해결력이 향상됨을 알 수 있었다. 한국교육개발원 언어 영재 교재나 국어 교과서 심화학습도 언어 영재에게 읽기 능력을 향상시키는 데 약간의 효과는 있지만, 언어 이해 능력과 언어 표현 능력을 효율적으로 발달시키는 데는 효과가 없는 것으로 나타났다. 한국교육개발원 언어 영재교재나 국어 교과서 심화학습 내용처럼 듣기, 말하기, 읽기와 쓰기를 따로 분리해서 지도하는 것보다 일상생활처럼 듣기, 말하기, 읽기, 쓰기를 종합해서 함께 지도하는 것이 언어적으로 재능있는 영재에게는 더 효과적이라고 나타났다. 또한 지역이나 학교 급별에 따라 언어 영재성의 개념이 달라질 수 있으므로, 그에 알맞은 언어 영재 심화학습 프로그램을 지속적으로 개발할 필요가 있는 것으로 나타났다. The purpose of this study is to verify empirically whether or not the linguistically creative problem-solving ability progress by developing and applying an enrichment learning program for the verbally gifted elementary school students. The total number of the verbally gifted students who participated in this study were 60 children. Half of them were subjects for evaluating the appropriateness of the enrichment learning program and the verbally creative problem-solving ability test developed by the researcher. The other half were divided into three groups for applying the teaching materials by Korean Educational Development Institute, the enrichment learning program by the present researcher, and the textbook enrichment program. The data were analyzed by the statistical method of repeated measures ANOVA. The results were as follows: First, the enrichment learning program developed by this researcher has greatly contributed to the advancement of verbally creative problem-solving ability. The program was especially effective in reading and writing, but not in speaking. second, the sub-category of reading includes factual, inferential·critical, and imaginary understanding. Among them, factual and imaginary understanding had not significantly advanced, while inferential·critical understanding had significant progression. Third, it was found that both logical and creative expression did not improve significantly by the enrichment learning program developed by the present researcher. Speaking is difficult to instruct. In conclusion, it was found that the systematic enrichment learning program was effective in developing language skills of the verbally gifted elementary school children. It was also proved to be more effective to teach them reading and writing together in everyday life. Also, it was turned out that continuous development of the enrichment learning program for the verbally gifted students is needed.

      • 폐결핵환자의 말초혈액 및 골수소견에 관한 혈액학적 연구

        최종오,최태열,최두혁,송계영 중앙대학교 의과대학 의과학연구소 1982 中央醫大誌 Vol.7 No.3

        To study the chronic anemia in pulmonary tuberculosis, hematologic examination including hemoglobin, hematocrit, RBC, MCV, MCH, MCHC, reticulocyte, serum iron, TIBC, serum ferritin and bone marrow aspiration were done in the patients of pulmonary tuberculosis who were not complicated by other chronic illness. The patients were devided into minimal and groups. The results obtained were as follows: 1. The pattern of chronic anemia in tuberculosis was normocytic and normochromia(MCV 92± 5.6fl, MCH 30 ±1.4pg, MCHC 31±1.1g/㎗ with significantly decreased hemoglobin, hamatocrit and RBC levels to 12.6±1.1gm/dl, 41±3.5% and 425±42 10^4/㎣, respectively in advanced group but those were not significanty decreased in minimal group. 2. Reticlocyte counts revealed slight increasing tendency to 1.1 0.4% in minimal group and decreasing tendcy to 0.6 0.2% in advanced group 3. Serum iron and TIBC were significantly decreased to 81±26㎍/dl and 263 ±49㎍/dl in advanced group but trancferrin saturation level was not changed. 4. Serum ferritin was significantly increased in advanced group to 136±25ng/dl. 5. Stainiable iron storage in bone marrow aspirate was slightlyelevated to +4 in 35% of minimal group and to +4 - +5 in 55% of advanced group. Also noted were mild erythroid hyperplasia in four of minimal group. 6. Therefore, it was suggested that the chronic anemia in pulmonary tuberculosis was to be resulted from mild suppression of normal erythropoietic response in bome marrow as the lesion was advanced in spite of rather increased storage of iron in bone marrow although mild compensation activity was seen in early stage.

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