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배양중에 있는 생쥐 여포난자(濾胞卵子)의 핵붕괴(核崩壞)(Germinal Vesicle Break-down)에 미치는 Progesterone의 영향에 관하여
조완규,권혁방,정순오,Cho, Wan-Kyoo,Kwon, Hyuk-Bang,Chung, Soon-O 대한생식의학회 1974 Clinical and Experimental Reproductive Medicine Vol.1 No.1
In the present studies, effect of progesterone on the germinal vesicle break-down of the mouse oocytes cultured in the micro tube was investigated. The results obtained are as follows: As dose of progesterone in the medium rose, accordingly the break-down of the germinal vesicle was suppressed. It was found that $ED_{50}$ was 15.7 ${\mu}g$/ml, and $ED_{90}$ 60.7 ${\mu}g$/ml of progesterone. The dose suppressing the oocyte maturation was apparently higher than that on the rabbit or on the mouse embryonal development. The inhibiting effect of progesterone on the GVBD was reversible. The germinal vesicle of the oocytes were broken down immediately in the medium upon removal of the hormone. Progesterone stops meiosis at any stage upon administration, while dbe AMP or theophylline supresses only the break-down of the nuclear membrane. Recovering of the meiotic division of the oocytes once exposed to progesterone was delayed a little. The inhibiting action of progesterone was not altered by adding more pyruvate or in the presence of higher concentration of the mineral ions in the culture medium.
착상전 생쥐배아의(GPI - 1)부계 동위효소 발현시기
조완규,박덕배,김성례 한국유전학회 1987 Genes & Genomics Vol.9 No.3
Present study was aimed to determine the pricise timing of the expression of paternal glucose phosphate isomerase (GPI-1) in preimplantation mouse embryos. Electrophoretic variants of GPI isozyme were analysed by cellulose-acetate electrophoresis. F_1 hybrid embryos obtained by crossing of Balb/c (GPI-lA) X CBA (GPI-1B) revealed that the paternally derived GPI-lA and heteropolymeric GPI-lAB bands were observed on day 5 of embryogenesis. The GPI-1 activity of tetraploid embryos induced by polyethylene glycol (PEG) -mediated fusion was compared with that of diploid embryos. While no substantial difference in GPI expression was observed in the diploid and tetraploid embryos until day 3, a 40% increase in GPI activity of tetraploid embryos on day 5 was apparent when compared with that shown in diploid embryos. These observations indicate that GPI synthesis in the earlier embryo might be derived from the maternal mRNA stored in the embryonic cytoplasm and the paternal genomic activation for GPI initiated on day 5 of embryogenesis where blastocysts form.