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Nasted PCR을 통한 참다래 궤양병균(Pseudomonas syringae pv.actinidiae)의 검출
정재성,고영진,한효심,조윤섭 한국식물병리학회 2003 식물병연구 Vol.9 No.3
A PCR method that combines biological and enzymatic amplification of PCR targets was developed for the detection of Pseudomonas syringae pv. actinidiae on kiwifruit leaves. A nested PCR was performed with primers designed from the coding sequence of the cfl gene, which is involved in production of the phytotoxin coronatine. The first and second primer sets efficiently amplified expected 665 and 310-bp fragments, respectively. With two successive amplifications, as few as 20 CFU/ml of P. syringae pv. actinidiae could be detected on ethidium bromide-stained agarose gel. Leaf samples were collected from 4 kiwifruit trees showing yellow halo spots on leaves and incubated in pepton-sucrose broth for 12 h at 16oC before PCR amplification. Positive detection was obtained with one sample, which was proved as a diseased plant in the next spring.
정재성,이영종,김종홍 한국생태학회 1999 Journal of Ecology and Environment Vol.22 No.5
하천에서 tetracycline과 gentamicin 저항성 유전자인 tetQ와 aacC2의 분포를 알아보기 위해 순천지역의 하천수로부터 전체 세균군집의 DNA를 분석하였다. 배양되지 않는 세균의 저항성을 고려하여 1liter의 하천수에 들어 있는 전체 세균의 DNA를 freeze-thaw 방법으로 추출하여 PCR을 통해 표적 유전자의 출현 정도를 조사하였다. 그 결과 tetQ 유전자는 축산농장이 있는 제 1지점에서 가장 많은 것으로 나타난데 반해 aacC2 유전자는 하천의 하류인 제5지점에서 가장 많이 출현하였다. 이러한 결과는 항생물질 저항성 유전자가 수질의 오염원을 알 수 있는 표지로 사용될 수 있는 가능성을 시사한다. The occurrence of tetQ and aacC2 genes encoding tetracycline and gentamicin resistance determinant, respectively, was assessed in total bacterial community DNA isolated from Dongchon stream of Sunchon area. To examine the resistance potential of bacteria that were not cultured, total DNA from 1 liter of stream water was extracted by freeze-thaw method. The PCR technique was employed to determine the abundance of the target genes. The highest frequency of tetQ gene was obtained from site 1, located near the animal farms area, whereas the incidence of aacC2 was highest in site 5, the downstream area. These results showed that the occurrence of antibiotic resistance gene may be used as a convenient marker of water quality related to source.
소자의 수명 예측을 위한 Weibull Step-Stress Type-I Model
정재성,오영환 대한전자공학회 1995 전자공학회논문지-A Vol.32 No.6
This paper proposes the step-stress type-I censoring model for analyzing the data of accelerated life test and reducing the time of accelerated life test. In order to obtain the data of accelerated life test, the step-stress accelerated life test was run with voltage stress to CMOS Hex Buffer. The Weibull distribution, the Inverse-power-law model and Maximum likelihood method were used. The iterative procedure using modified-quasi-linearization method is applied to solve the nonlinear equation. The proposed Weibull step-stress type-I censoring model exactly estimases the life time of units, while reducting the time of accelerated life test and the equipments of test.
Pseudomonas syringae의 식물독소와 독소 생산 균주의 검출을 위한 PCR Primer
정재성,한효심,고영진 한국식물병리학회 2001 식물병연구 Vol.7 No.3
Many pathovars of the species Pseudomonas syringae are known to produce different phytotoxins as secondary metabolites. Although phytotoxins generally enhance the virulence of P. syringae, they are not required for pathogenesis. Among the phytotoxins produced by P. syringae, lipodepsipeptides, coronatine, phaseolotoxin, and tabtoxin are the most well-known toxins which have been intensively studied for their structure, mode of action, biosynthesis, and regulation. A polymerase chain reaction (PCR) technique that amplifies a segment of the phytotoxin gene cluster using a primer set has been developed in recent years. This method offers the advantages of speed and sensitivity compared to the approaches based on physiological and biochemical methods. PCR detection of genes involved in the production of toxins could be exploited for early diagnosis of plant diseases caused by P. syringae pathovars.
정재성,이재형 한국수자원학회 1996 물과 미래(한국수자원학회지) Vol.29 No.3
남한강 상류 평창강 유역을 대상으로 기상레이더 자료와 지상강우량 자료를 이용한 시우량 곡면 예측 방법을 연구하였다. 레이더 자료의 이용시에 필요한 비정상 에코와 지형에코의 제거, 강우에 의한 감쇄 효과의 보정 방법, 레이더 반사도 Z와 강우강도 R의 관계, 등을 검토분석하였다. 보정된 레이더 반사도 자료를 레이더 우량곡면으로 변환하고, 그 결과와 지상강우량 자료를 이용하여 시우량곡면을 합성하였다. 시우량곡면 예측을 위하여 상호상관 계수법을 이용한 강우장의 이동성과 유역 평균 레이더 강우강도의 시변성을 고려하였다. 이 예측방법을 합성된 시우량곡면 자료에 적용하여 3시간까지의 시우량곡면을 예측하고, 예측된 결과를 지상우량 및 합성우량 곡면과 비교하였다. 본 연구의 단시간 강우예측 방법은 보다 많은 자료를 이용한 검증과 강우장의 물리적 특성 및 지형을 고려한 방법으로의 보완이 필요한 것으로 사료되었다. In this study, a methodology for the hourly prediction of rainfall surfaces was applied to the Pyungchang river basin at the upstream of South Han river with meteorological radar and ground rainfall data. The methods for the exclusion of abnormal echoes, and suppression of ground clutter, and the augmentation of attenuation effects associated with rainfall phenomena were reviewed, and the relationship between radar reflectivity (Z) and rainfall rate (R) was analyzed. The transformation of augmented radar reflectivities into the rdar rainfall surfaces was carried out, and afterward they were synthesized with the ground rainfall data generating the hourly rainfall surfaces. For the prediction of hourly rainfall surface, the moving factors of rainfall field estimated by the cross correlation coefficient method and the temporal variation of radar rainfall intensities were considered. The synthesized hourly rainfall surfaces were used to predict the hourly rainfall surfaces up to 3 hours in advance and subsequently the results were compared with the measured and the synthesized. It seems that the prediction method need to be verified with more data and be complemented further to consider the physical characteristics of rainfall field and the topography of the basin.