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정구민,권정숙 安東大學校 農業科學技術硏究所 1998 農業科學技術 硏究論文集 Vol.5 No.-
마첨가 국수의 개발을 위해 밀가루에 열풍건조마가루와 냉동건조마가루를 각각 2, 5, 10% 첨가하여 건국수를 만들고, 이들의 물리적 성질과 조리성질을 조사하였으며 관능검사를 실시하였다. 밀가루에 마가루를 첨가하면 국수의 폭은 0.5-4.8% 두께는 8.8-20.8% 줄어들었다. 국수의 색은 마가루의 갈변으로 다소 누렇게 변하였으며 첨가량이 클수록 심하였다. 마첨가로 국수의 강도와 휨성은 증가하였으며, 이에 따라 마국수는 밀가루 국수보다 취급이 용이할 것으로 생각된다. 국수의 조리 후 무게증가량과 조리부피는 마첨가량에 따라 별 차이를 보이지 않았으나, 조리손실은 마첨가 국수가 1.21-1.49g/25g으로 밀가루 국수의 1.15g/25g 보다 높았다. 이는 마의 수용성 식이섬유 함량(4.74-4.96%)이 밀가루(2.69%)보다 높기 때문일 것이다. 관능검사 결과, 마가루를 2-5% 첨가한 국수가 5.4-6.4점을 얻어 밀가루 국수의 5점보다 높았다. Dried noodles were prepared by blending wheat flour with 2, 5, 10% hot-air dried Chinese yam powder (HD) and freeze dried Chinese yam powder (FD), respectively. Adding yam powder decreased width and thickness of noodles by 0.5-4.8% and 8.8-20.8% respectively. Color of noodles were darkened with addition of yam powder due to probably enzymatic browning. Adding yam powder increased strength and flexibility of noodles. Weight gain and volume of noodle after cooking were not affected by adding yam powder, but cooking loss was increased from 1.15g/25g for wheat noodle to 1.21-1.47g/25g for yam-added noodles because yam powders contain more soluble dietary fiber(4.74-4.96%) than wheat flour(2.69%). In sensory evaluation, noodles added with 2-5% HD and FD scored 5.4-6.4 point whereas wheat flour noodle was 5.
투명대 존재/부재 햄스터 난자의 동결보존;1-단계 평형과 2-단계 융해의 효과
정구민,방명걸,김석현,신창재,김정구,문신용,이진용,장윤석,Chung, K.M.,Pang, M.G.,Kim, S.H.,Shin, C.J.,Kim, J.G.,Moon, S.Y.,Lee, J.Y.,Chang, Y.S. 대한생식의학회 1992 Clinical and Experimental Reproductive Medicine Vol.19 No.2
The present experiments were focussed to modify a short slow-cooling protocol used for freezing of early stage embryo(Testart et al., 1986) and also to apply the modified method for the cryopreservation of hamster oocytes with Zona or without. The protocol was modified by changing the 4-step equilibration into 1-step and the 1-step thawing into 2-step. The oocytes were added in 1.5M PROH and 0.1M Sucrose, seeded at $-7^{\circ}C$, slow cooled($0.3^{\circ}C$/min) to $-30^{\circ}C$ before plunging to $-196^{\circ}C$. The oocytes were thawed at $23-25^{\circ}C$ air(20sec/150sec) and/or 33-35 water(10sec). The survival of the frozen-thawed oocytes was determined by morphologic criteria and their fertilizing ability was also estimated by Sperm Penetration Assay(SPA) system(Chang et al, 1990) using fertile men semen sample. One-step equilibration showed slightly higher survival rate(83.9% vs. 71.0%) and fertilization rate(83.9% vs. 71.0%) compared with four-step(p>0.05). And two-step thawing(air & water exposing) of oocytes frozen after 1-step equilibration showed significantly higher survival rate(96.3%) than one-step thawing at air(85.2%) or water(65.0%) only(p<0.05). Therefore, by the modified method(l-step equilibration & 2-step thawing), Zona-intact(ZI) and Zona-free(ZF) oocytes were frozen and thawed. ZI-oocytes showed significantly higher survival rate(95.4%, 308/323 vs. 67.6%, 240/355) than ZF-oocytes(P<0.01). But the survival of ZF-oocytes was as high as ZI-oocytes in fourteen of twenty-four replicates. ZI-oocytes was also significantly higher fertilization rate($92.4{\pm}8.9%$ vs. $63.7{\pm}18.5%$) and higher mean number of penetrated sperm($6.2{\pm}4.2$ vs. $3.9{\\pm}3.3$) than ZF-oocytes, but not higher than control(fresh oocytes;$99.3{\pm}2.4%$, $8.4{\pm}4.2$)(P<0.001). Conclusively, this modified method will contribute to freeze effectively the hamster oocytes for simplifing of the logical consideration of performing SPA and also to freeze the human and other animal oocytes.
체액이 초기배의 발생생리에 미치는 효과에 관한 연구 1 : 생쥐 1- 및 2-세포배의 체외발생에서 배양액과 단백질원의 효과
정구민,임경순 韓國受精卵移植學會 1991 한국동물생명공학회지 Vol.6 No.1
In vitro developmental ability of early preimplantation monse embryos was shown to be depend on the embryonic stages, media and snpplements and their interaction(Experiment 1). The development of I-cell embryos were more promoted in the complex medinm(Ham's Fl0) than in the simple one(m-KRB), but that of 2-cell embryos showed the reverse effect. The bovine serum albumin(BSA) as a medium snpplement more promoted the development of I- and 2-cell embryos, compared with human fetal cord serum(HCS). On the other hand, the harmful effect of HCS was especially shown on the early cleavage in the embryonic development of the two stages. The effect of serum, in the respect of interaction between media and snpplements. was also more significantly appeared in m-KRB than Ham's Fl0. In the experiment 2, when the harmful effect of HCS was compared with that of fetal bovine serum(FBS), the former more promoted the development of l - and 2-cell embryos than the latter. The effect of HCS was more significantly shown in the development of I-cell than that of 2-cell embryos. Conclusively, as I- and 2-cell embryos were different in the requirements for the in vitro development. the optimal medium and supplement have to be selected for each embryonic stage. It is also respected to the better result if it take into consideration into the kinds of sera when serum is used for culture of early preimplantation embryos.
정구민,정의용,정혜영,장명준,jeong, Gu-Min,Joung, Eui-Young,Jung, Hea-Young,Jang, Myoung-Jun 한국버섯학회 2021 한국버섯학회지 Vol.19 No.3
국내 표고 품종 다양화로 버섯산업 경쟁력을 높이고자 '참아람'과 청흥버섯영농조합 보유균주인 'E140025'을 이용하여 교잡육종법을 통해 '청흥1호'를 육성하였다. 자실체 특성 결과 대조품종 보다 갓직경은 작고, 대길이는 길고, 대가 굵어 개체중이 무거웠다. 초발이기간은 '청흥1호' 24일, '설백향' 4일, '산조701호' 8일로 발이까지 대조품종보다 20일정도 늦었다. 자실체 수량은 가장적게 나타났지만, 유효수량은 '청흥1호' 6,498 g '설백향' 4,290 g, '산조701호' 5,022 g으로 가장 높았다. URP-Primer를 이용한 다형성검정을 통해 6개의 Primer에서 모본과 다른 PCR 밴드를 확인하였다. 이상의 결과, 재배기간이 길지만 개체중과 유효수량이 우수한 무포자 신품종을 육성하였다. The competitiveness of the mushroom industry can be increased by diversifying the domestic Lentinula edodes cultivar. Therefore, 'Chungheung 1ho' was cultivated by hybrid breeding method using strain 'E140025,' which was collected by the Chungheung Mushroom Farming Association. Regarding the morphological characteristics of the fruit, the cap of 'Chungheung 1ho' was smaller than that of the control cultivar, whereas, its stem was longer and thicker than that of the control cultivar. In addition, the weight of the individual fruits of the new cultivar was heavier than that of the control cultivar. The period for primordia formation was 24 days for the new cultivar, which was 16-20 days later than that of the control cultivar. Furthermore, it was found that the patterns of the PCR amplification band using six URP primers were different for 'Chungheung 1ho' than those of the mating strains.
유비쿼터스 멀티미디어 컨텐츠의 자기 네트워킹과 대체 구조에 대한 연구
정구민,박경준,가충희,안현식,문찬우,Jeong, Gu-Min,Park, Kyung-Joon,Ka, Chung-Hee,Ahn, Hyun-Sik,Moon, Chan-Woo 한국융합신호처리학회 2007 융합신호처리학회 논문지 (JISPS) Vol.8 No.4
본 논문에서는 유비쿼터스 멀티미디어 컨텐츠의 구현을 위해서 자기 네트워킹과 대체 구조를 제안한다. 유비쿼터스 멀티미디어의 여러 가지 특성 중에서 컨텐츠의 측면에서는 컨텐츠의 사실성과 실시간성이 매우 중요하다. 컨텐츠의 실시간성을 위해서 본 논문에서는 자기 네트워킹과 대체 구조를 구현하고 해석한다. 컨텐츠의 네트웍 기능을 이용하여 서버에 접속하고 스스로 업데이트 할 수 있도록 한다. 이에 따라서 전체적인 데이터 전송량을 줄일 수 있으며 마코프 체인 모델을 이용하여 제안된 구조의 처리율을 해석하여 효용성을 보인다. 또한 제안된 구조의 단말기에서의 구현과 이를 통한 어플리케이션에 대해서 정리한다. In this paper, we propose a novel self-networking and replaceable structure method for the ubiquitous multimedia. As the contents in the ubiquitous multimedia should be realistic and continuously updated in the real-time manner, an efficient scheme of a self-networking and replaceable structure is necessary. In the proposed method, the contents itself connects to the server or corresponding devices and updates itself autonomously. Also, we can reduce the total amount of data transmission comparing to the cases where the whole contents should be downloaded. A Markov chain model is introduced for the proposed structure in order to perform the throughput analysis. The whole mechanism is implemented in the wireless handset and also, various applications of the scheme are discussed.