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유용 2차 대사산물의 (代謝産物) 생산을 위한 약용식물의 증식과 형질전환 - 1. 기내배양을 (器內培養) 통한 병풀 엽병의 식물체 재분화
김광수(Kwang Su Kim),백윤웅(Yun Woong Paek),고경민(Kyung Min Ko),황성진(Sung Jin Hwang),임병탁(Hyun Tag Im),황백(Baik Hwang) 한국식물학회 1995 Journal of Plant Biology Vol.38 No.2
Somatic embryogenesis and subsequent plantlet formation were obtained from embryogenic calli derived from young petioles of Centella asiatica. Embryogenic callus cultures were induced from petiole explants of Centella asiatica on basal MS medium supplemented with 1.0 ㎎/L 2,4-D and 1.0 ㎎/L kinetin. After transfer of these calli to MS medium supplemented with 5.0 ㎎/L NAA and 1.0 ㎎/L kinetin, 87% of embryogenic calli were differentiated into embryoids with primary and adventitious roots, elongated hypocotyl and cotyledon. Plantlets were induced when these embryoids were transferred to half strength inorganic salts of basal MS supplemented with 0.2 ㎎/L NAA and 0.2 ㎎/L kinetin.