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동결-해동-원심분리를 이용한 무세포 돼지각막의 특성 연구
이석현,전연숙,김재찬.Seok Hyun Lee. MD. Yeoun Sook Chun. MD. Jae Chan Kim. MD. PhD 대한안과학회 2011 대한안과학회지 Vol.52 No.1
Purpose: To develop a new decellularization technique of porcine cornea using freezing-thawing-centrifugation (FTC) and to examine the characteristics of acellular porcine cornea (APC) for xenograft material. Methods: Two-hundred micrometer thickness porcine corneas were decellularized with DNase/RNase, followed by 3 freezing-thawing-centrifugations (FTC, group 1), lyophilized FTC-APC (group 2), and chemical enzyme treated APC (CE-APC, group 3). Histologic evaluation to examine cells and collagen matrix, comparison of transparency, and cultivation to determine the viability of stromal cells was performed in fresh porcine cornea and 3 experimental groups. Results: Decellularization occurred successfully in all experimental groups. Decellularization was confirmed by H&E staining and cultivation. Transparency of group 1 was similar to the normal porcine cornea but transparency of group 2 and group 3 was decreased. Collagen fibers of CE-APC (group 3) were not as well arrayed as FTC-APC (group 2). Conclusions: Acellularity of porcine cornea was successfully achieved by the FTC method with preservation of the cornea stroma. Novel decellularized porcine cornea can be considered as xenogeneic material for corneal transplantation. J Korean Ophthalmol Soc 2011;52(1):86-92
이석현,김재훈,김지택,전연숙,김재찬,Seok Hyun Lee,Jae Hoon Kim,Jee Taek Kim,Yeoun Sook Chun,Jae Chan Kim 대한안과학회 2008 대한안과학회지 Vol.49 No.7
Purpose: To report a case of ocular surface reconstruction using nasal and oral mucosa auto grafts on a man whose ocular surface was deformed by a chemical burn. Case summary: A 48-year-old man was injured when his right eye was exposed to alkali solution. He underwent fifteen operations, including amniotic membrane auto grafts and fornix reconstructions. However, his right eye still suffered from symble pharon, drying, corneal neovascularization, and erosion. We performed ocular surface reconstruction with free autologous nasal and oral mucosal transplantation and amniotic membrane graft. By one month after surgery, his eye showed no evidence of symble pharon or fibrovascularization recurrence on the cornea or conjunctiva. By six months after surgery, his ocular surface had stabilized. Conclusions: Ocular surface reconstruction with free autologous nasal and oral mucosal transplantation is a useful method on eyes that are injured by chemical burns. J Korean Ophthalmol Soc 49(7):1177-1182, 2008
인천광역시 원도심 공원녹지 기본계획 현황 및 이용 평가
이석현,김하늘,곽동화,Lee, Seok-Hyun,Kim, Haneul,Kwack, Dongwha 인천대학교 도시과학연구원 2018 도시과학 Vol.7 No.2
The purpose of this study is to review and evaluate the proper planning for 2030 Incheon Urban Parks & Green Space Master Plan. Some of the plans are aimed at multiple small districts within the city's old town, as local governments are planned to be sub-subsidized by Incheon Metropolitan City. However, these plans have become simple plans for each district and therefore find it difficult to collect from the perspective of the using system of the residents who are directly used. First, the construction of the urban park and green space network requires the construction of a service using system that can be felt by the users. Second, the basic idea of each district planned as the detailed strategy of the master plan should be planned through consultation with nearby the district. Third, specific targets and directions for the urban park and green space plan through the urban development project are needed.
이석현(Seok-Hyun Lee),안지현(Ji-Hyun Ahn) 대한정형외과학회 2008 대한정형외과학회지 Vol.43 No.1
외상후 발생한 하지동맥손상은 주로 광범위한 연부조직 손상을 동반하는 압궤손상이나 총기류 등에 의한 관통상에서 보고되고 있으며 대부분 외상후 초기에 발견되어 조기 혈관봉합술이나 재건술을 거치게 된다. 하지만, 진단이 지연된 경우 외상후 수주내지 수개월에 가성 동맥류로 발견되기도 한다. 대퇴골 골절 후 발생할 수 있는 가성 동맥류는 주로 표재 대퇴동맥에서 발생하며 근육 심층부로 깊게 주행하며 내측 광근에 의해 보호되는 심부 대퇴동맥에서는 발견하기 힘들다고 알려져 왔다. 외상후 하지에 발생한 가성 동맥류에 대해서는 많은 보고가 있어 왔지만 주로 표재 대퇴동맥에 국한되어 있으며 초기 정상인 하지동맥 조영검사 결과를 보인 경우는 드물다. 수상 직후에 시행한 하지동맥 조영검사에서 정상소견을 보인 대퇴골 개방성골절에서 수상후 4주에 하지 동맥 조영검사를 반복하여 진단하고 치료한 심부 대퇴동맥의 가성 동맥류 1예를 보고하는 바이다. Post-traumatic arterial injuries are often reported in crushing or penetrating gun-shot injuries that include massive soft tissue damage. In most cases, the injured arteries are treated with early repair or reconstruction surgery. When there is a delayed in diagnosis, a pseudoaneurysm might be detected several weeks or months after the trauma. Pseudoaneurysms after femoral fracture are usually detected in the superficial femoral arteries, but rarely in the very deep femoral arteries, which are protected by the vastus medialis. There are many reports on post-traumatic pseudoaneurysms, most of which are in the superficial femoral arteries, and there are few reports with normal findings in an early angiogram. We report a pseudoaneurysm of the deep femoral artery with a normal angiogram in the early stage that was diagnosed and treated 4weeks after the trauma.