화학적으로 변형된 하수 슬러지를 이용한 반응성 염료의 생물흡착

윤영상,한민희,최기욱 한국청정기술학회 2007 청정기술 Vol.13 No.3

Biosorption is considered to be a promising alternative to replace the present methods for the treatment of dye-containing wastewater. In this study, sewage sludge was used as a biosorbent which could be one of the cheapest and most abundant biomaterials. The objective of this work is to develop a surface-modified biosorbent with enhanced sorption capacity and binding affinity. The FT-IR and potentiometric titration studies revealed that carboxyl, phosphateand amine groups played a role in binding of dye molecules. The binding sites for reactive dye Reactive Red 4 (RR 4) were identified to be amine groups present in the biomass. In this work, based on the biosorption mechanism, the performance of biosorbentcould be enhanced by the removal of inhibitory carboxyl groups from the biomass for practical application of the biosorbents. As a result, the maximum capacity of biomass was increased up to 130% and 210% of the increment of sorption capacity at pH 2 and 4, respectively. Therefore, chemically modified sewage sludge can be used as an effective and low-cost biosorbent for the removal of dyes from industrial discharges. 생물흡착은 염색폐수로부터 염료를 제거하기 위한 기술로서 현재 사용되고 있는 기술을 대체할 수 있는 유망한 처리 방법이다. 본 연구에서는 생물흡착제로써 저가이면서 풍부한 소재중의 하나인 하수 슬러지를 이용하였다. 본 연구의 목적은 바이오매스의 변형을 통하여 흡착능력을 향상시키는데 있다. FT-IR 분석과 적정실험을 통하여 흡착에 관여하는 작용기는 카르복실 그룹, 인산 그룹, 아민 그룹으로 판명하였으며 그 중에서 반응성 염료(Reactive Red 4, RR 4)를 흡착할 수 있는 작용기는 아민 그룹임을 알 수 있었다. 또한 음이온성 염료인 RR 4의 흡착을 저해하는 것으로 생각되는 카르복실 그룹을 제거함으로써 흡착성능을 향상시킬 수 있었다. 그 결과, 카르복실 그룹이 제거된 바이오매스의 최대 흡착량이 변형 전에 비해 pH 2에서는 130%, pH 4에서는 210% 증가하였다. 그러므로 화학적으로 변형시킨 하수 슬러지는 산업폐수내 염료제거에 효과적이면서 값싼 생물흡착제로 이용될 수 있을 것으로 기대된다.

에너지 저장 시스템(ESS)의 경제성 분석 기법에 관한 연구

윤영상,최재현,최용락,신용태,김종배,Yoon, Young-Sang,Choi, Jae-Hyun,Choi, Yong-Lak,Shin, Yongtae,Kim, Jong-Bae 한국정보통신학회 2015 한국정보통신학회논문지 Vol.19 No.3

Recently, the government is promoting the new renewable energy spread and expansion policy. To this end, the investment and the research is ongoing on the core of the ESS (Energy Storage System) for the Smart Grid that is being spread around the industrialized countries. US and European countries have also conducted a variety of ESS related systems maintenance and improvement in order to induce the activation of the ESS industry. On the other hand, our country has no law and institutional foundation for the introduction of activation ESS, and there is no objective basis for the economic impact of the introduction of the ESS. Therefore, spread and activation of the ESS is not properly conducted. In this paper, the economics of the ESS based on the Korea electric pricing system for the spread and activation of the ESS effectively proposes a technique for analysis. To do this, define the ESS operating model, and propose the best economic analysis method economic analysis comparing each operating model. 최근 정부가 추진하고 있는 신재생에너지 보급 및 확대 정책으로 스마트그리드의 핵심인 ESS에 관한 투자 및 연구가 선진국을 중심으로 빠르게 확산되고 있다. 미국을 비롯한 유럽 선진국들은 다양한 ESS관련 제도정비 및 개선을 통해 ESS 산업의 활성화를 유도하고 있는 반면, 우리나라의 경우, ESS 도입 및 활성화를 위한 법제도적 기반이 마련되어 있지 않다. 또한, ESS도입에 대한 경제적 효과성에 대한 객관적인 기준이 없어, ESS의 보급 및 활성화가 제대로 이루어지지 않고 있다. 이에 본 연구에서는, ESS의 보급과 활성화를 위해 한국의 전기요금 체계를 기반으로 ESS의 경제성을 효과적으로 분석하기 위한 기법을 제시한다. 이를 위해 ESS의 운영 모형을 정의하고 각 운영 모형별 분석기법을 비교하여 최적의 경제성 분석방법을 제시한다.

염류내성관련 유전자 Betaine Aldehyde Dehydrogenase Gene의 인삼 체세포 배발생을 통한 형질전환

윤영상,송원섭,양덕춘,배창휴,윤재호 한국자원식물학회 2005 한국자원식물학회지 Vol.18 No.1

Korean ginseng(Panax ginsengC.A. Meyer) is very difficult to obtain stable production ofqualified ginseng roots because of variable stresses in soil environments. In transformationof ginseng with betain aldehyde dehydrogenase gene, compounds synthesized for controllingosmotic pressure such as proline, glycine, betaine, polyols and sugar were accumulated incell for salt resistance in transgenic plants. 2 Agrobactgeriumconjugants were acquired withbetA and betB genes for solt resistant plants. A. tumefaciens MP90/pBetAand A.tumefaciens MP90/pBetBwere recombined for increasing the tolerance to salt stress. Toconfirm the transformation of the binary vector, tobacco plant was transformed, and thetransformant can grow on media containing high concentration of kanamycin. To identifyNPT II, BetAand BetBgenes of the transformants, the band on the agarose was confirmedby PCR and RT-PCR techniques. The transformants of ginseng with betA and betB geneswere acquired on the phytohormone free basic MS media containing only antibiotics and 1M mannitol used for selection of transgenic plant, but the transfomation efficiency for BetAand BetBwas very low. 염류내성 식물은 염류농도의 변화에 따라 세포내의 삼투압을 유지하기 위한 화합물을 합성하는 기작을 가지고 있는데 이런 화합물은 주로 proline, glycine, betaine, polyols, sugar등으로 체내에 축적함으로서 고농도의 염류에 견디는 것으로 알려져 있다. Betaine은 미생물에서 2단계 반응을 통해 choline에서 합성되는데, 첫단계는 choline dehydrogenase(CDH)에 의해서 촉매되고(Bet A gene), bet B 유전자의 산물인 betaine aldehyde dehydrogenase(BADH)에 의해 수행된다. 본 실험에서는 Bet A, Bet B 유전자를 아그로박테리움에 도입하여 새로운 conjugants 2종을 획득하였으며(Agrobacterium tumefaciens MP90/pBet A, Agrobacterium tumefaciens MP90/pBet B), 먼 저 재조합된 binary vector가 식물에서 발현 및 형질 전환되는지 여부를 조사하기 위해서 이미 담배에 형질전환을 시켰으며, 형질전환된 담배에서는 고농도의 kanamycin배지에서 생장이 가능하였고, PCR에 의하여 NPT II, Bet A, Bet B gene를 조사한 결과 담배 유식물체 모두 band가 형성되어 형질전환체임을 확인할 수 있었다. 인삼에 Bet A, Bet B gene의 도입은 1M의 mannitol이 함유된 식물호르몬 무첨가 MS 배지에서 단일배 발생방법에 형질전환체를 획득하였으나, 형질전환체의 발생빈도(12%)가 매우 낮았다.

Biosorption-based recovery of precious metals 생체흡착기술을 이용한 귀금속 회수

윤영상 한국공업화학회 2012 한국공업화학회 연구논문 초록집 Vol.2012 No.1

Ferritin Light Heavy Chain 유전자가 도입된 인삼형질전환체의 단일배발생을 통한 식물체의 기내증식

윤영상,양덕춘,김무성,김종학 한국자원식물학회 2004 한국자원식물학회지 Vol.17 No.2

Optimal regeneration conditions of ginseng transformants were studied. It has beenknown that Ferritin Light Heavy Chain (FLHC) gene remove the several heavy metal bycombination, store and transport. To obtain the ginseng tolerant to heavy metal, binaryvector was introduced in Agrobacteriumby tri-parental mating and then AgrobacteirumtumefaciensMP90/FLHC was selected on the AB media and MS media containingkanamycin. Explants were co-cultured with Agrobacterium tumefaciensMP90/FLHC, whichcontained NPT II as a selectable marker, tadpole ferritin heavy chain (FLHC) gene andhuman ferritin light chain gene and then a number of embryos were induced. The inducedembryo transferred to shooting media consisting of MS medium supplemented with GA 10mg/L. As a result of examination that induced the normal growth of transfomants,transformants showed the equivalent growth in both root and shoot on the media containingthe 1/3 MS.

배배양에 의한 인삼 우수계통으로부터 염류 Stress 내성 계통의 선발

윤영상,양덕춘,김무성 한국자원식물학회 2004 한국자원식물학회지 Vol.17 No.3

Selection of stress-tolerant ginseng lines in fields is very difficult because it is almostimpossible to control properly the environmental conditions of soil. On the contrary, it canbe studied with ease to search for stress-tolerant ginseng lines through in vitroculturebecause of easy manipulation of stress conditions. This study was conducted for the selectionof ginseng pure lines tolerant to salt stress. Murashige & Skoog(MS) media with 2.5 folds ofKNO3, NH4NO3, MgSO4· 7H2O, KH2PO4, and CaCl2· 2H2O was established for the selectionof ginseng pure lines tolerant to salt stress in vitro. Among 88 ginseng pure lines bred byKorea Ginseng and Tobacco Research Institute, Punggi Hwangsuk, 78093, 82886, 78135,86024 and KG104 lines was tolerant to salt stress. For the stable production of qualityKorean ginseng, genetic tolerance to salt stress is one of important factors since relativelyhigh salt concentrations in the ginseng nursery soil environment of Korea. Ginseng inbredpure lines were tested for their tolerance to salt stress through in vitroculture technique.

연구실 소개 : 전북대학교 환경생물공학연구실 -고성능 선택특이성 생체흡착소재의 설계 기술 개발-

윤영상 한국화학공학회 2009 NICE Vol.27 No.2

Heating에 의한 UO2-Zr system의 화학적 변화

윤영상,김종구,박순달,하영경,송규석 한국방사성폐기물학회 2014 한국방사성폐기물학회 학술논문요약집 Vol.12 No.1

혼합 용매에서의 겔화/결정화에 의한 초고분자량 폴리에틸렌 필름의 역학적 성질

윤영상,조재환 한국섬유공학회 1996 한국섬유공학회지 Vol.33 No.1

Ultra-high molecular weight polyethylene (UHMWPE) films with a molecular weight of 6×10 were produced by gelation/crystallization from solution with cosolvent mixtures of decalin and p-xylene. Prior to the analysis of mechanical properties of gel films, sol-gel transition temperature was measured, being dependent on the volume fraction of mixed solvents and polymer concentration. Critical polymer concentration where there is an abrupt change in the concentration dependence of sol-gel transition temperature was higher for xylene-rich solution than for decalin-rich solution. Young's modulus and tenacity of gel films increased with the increase of draw ratio, but were not affected significantly by the volume fraction of mixed solvents. Bire-flingence of gel films increased initially with the increase of draw ratio, but reached an equilibrium value above some critical draw ratio. Critical draw ratio and equilibrium hire-flingence of gel fem for xylene-rich solution were higher than those for decalin-rich solution. Young's modulus tended to increase with the birefringence, but did not show any dominant dependence on the crystallinity.

가압경수로형 핵연료의 연소이력에 따른 핵분열생성기체의 방출특성

윤영상,박순달,하영경,송규석 한국방사성폐기물학회 2014 한국방사성폐기물학회 학술논문요약집 Vol.12 No.1