A Case of Using Hominis Placenta and Soyeom Mixed Pharmacopuncture to Remove Melanocytic Nevus

서형식 대한약침학회 2018 Journal of pharmacopuncture Vol.21 No.1

Objective: It has been attempted to find out that Homine Placents and Soyeom mixed Pharmacopuncture (HSP) procedure is effective for the removal of melanocytic nevus of considerable size which cannot be applied to general acupuncture. Methods: Hominis placenta (0.5ml) and Soyeom (0.5ml) mixed pharmacopuncture (1.0ml) was subcutaneously injected in several places of melanocytic nevus. The treatment was performed about 20 times, once a week. Layer analysis of pigmentation was carried out using ECOSKIN. Results: One year after the HSP procedure, the pigmentation of the melanocytic nevus was slightly reduced and completely removed after 2 years of procedure. Conclusion: The HSP could be effective for removing hyperpigmentation in the melanocytic nevus.

紫何車 藥針液이 B16 melanoma 세포주의 멜라닌 합성에 미치는 영향

서형식 대한약침학회 2006 Journal of pharmacopuncture Vol.9 No.1

청대(靑黛)와 생지황(生地黃)의 항염 및 항산화 효과에 대한 실험적 연구

서형식,Seo, Hyeong-Sik 대한한방안이비인후피부과학회 2008 한방안이비인후피부과학회지 Vol.21 No.3

Objectives : This experiment study was performed to investigate the anti-inflammation and anti-oxidant effects of Indigo Naturalis(IN) and Rehmanniae Radix(RR) which were herbs for clearing heat. Methods : Anti-inflammation and anti-oxidation effects of IN and RR that were measured by the inhibitory ability of Nitric oxide(NO) production and the scavenging for 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical. Results : 1. IN and RR groups were not showed cell toxicity. 2. In the inhibitory ability of NO production, IN groups were better than RR groups, but there were no statistical significances among the groups. 3. In the scavenging for DPPH radical, IN groups were better than RR groups, but there were no statistical significances among the groups. Conclusions : These results showed that the IN group was better than the RR group in treating skin eruptions, especially those such as erythema, purple spot, papule, pustules which appear in acute inflammation.

자하거(紫何車) 약침액(藥針液)이 B16 melanoma 세포주의 멜라닌 합성에 미치는 영향

서형식,Seo, Hyung-Sik 대한약침학회 2006 Journal of pharmacopuncture Vol.9 No.1

Objective : This research was carried out for the development of medicine for vitiligo treatment and focused on the effect of Hominis Placenta extract on Melanin synthesis of B16 melanoma cells. Methods : Acitivity of tyrosinase playing a vital role in synthesis of Melanin and the quantity of Melanin, which is the final product in cultured B16 melanoma cells, effects of Hominis Placenta extract were measured. Results: The results indicated that Hominis Placenta extract increased both the amount of Melanin and the activity of tyrosinase according to the concentration, and they also supported by western blot analysis. Conclusion : The results suggests that Hominis Placenta extract has an advantageous effect on the promotion of Melanin synthesis and will contribute to the development of vitiligo treatment through further related studies.

消風散의 Allerhy 抑制 效能에 관한 硏究

서형식,노석선,Seo, Hyung-shikk,Roh, Seok-sun 대한한방안이비인후피부과학회 2000 한방안이비인후피부과학회지 Vol.13 No.1

This study was carried out to prove the effects of SPS on antioxidants, iching by histamine in human model and skin stimulus. The result were obtained as follows : 1. Antioxidants of SPS is better than Vitamine C and is not less than BHT(Dibutylated Hydroxytoluene). 2. Inhibitory effect of iching by histamine is better than Dermobate. 3. Skin stimulus is not in ROAT(Repeated Open Application Test).

개용환(改容丸)의 항염 및 항산화 효과에 대한 실험적 연구

서형식,Seo, Hyeong-Sik 대한약침학회 2009 Journal of pharmacopuncture Vol.12 No.3

Objectives : This study was performed to investigate the anti-inflammatory and anti-oxidantic effects of GaeYongHwan(GYH) extract which has been used for patients with acnes. Methods : Anti-inflammatory and anti-oxidantic effects of GYH extract were tested in terms of inhibitory ability of Nitric oxide(NO) production, 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging activity and anti-bacterial effects against Propionibacterium acnes(P. acnes). Results : 1. All GYH treated groups did not show cytotoxicity. 2. Treatment with $100{\mu}g/m{\ell}$ of GYH extract lowered production levels of NO significantly compared to non-treated control or normal. 3. All of GYH treated groups did not show DPPH free radical scavenging activities. 4. All of GYH treated groups did not show anti-baterial action against P. acnes. Conclusions : These results imply that GYH extract has anti-inflammatory effect to treat acnes.

황련해독탕 약침액과 전탕액이 Staphylococcus aureus와 Staphylococcus epidermidis에 미치는 항균효과에 대한 실험적 연구

서형식,Seo, Hyung-Sik 대한약침학회 2006 Journal of pharmacopuncture Vol.9 No.2

Objective : This experimental study was performed to investigate the effect of Hwangryunhaedoktang Herbal-acupuncture & extract on Staphylococcus aureus and Staphylococcus epidermidis that induce keratitis. Methods : MIC was measured by dropping to $50{\beta}^{\circ}$ according to density Hwangryunhaedoktang Herbal-acupuncture & extract(100%, 10%, 1%, 0.1%). Anti-bacterial potency was measured by the size of inhibition zone With change of volume. Results : 1. MIC on Staphylococcus aureus in Hwangryunhaedoktang Herbal-acupuncture & extract were not disappear all sample. 2. MIC on Staphylococcus epidermidis in Hwangryunhaedoktang Herbal-acupuncture & extract were not disappear all sample. Conclusions : The present author think that Hwangryunhaedoktang Herbal-acupuncture must be extract a new method for anti-bacterial potency.

황련의 증량에 따른 전탕액이 Staphylococcus aureus에 미치는 항균효과에 대한 실험적 연구

서형식,Seo, Hyeong-Sik 대한약침학회 2007 Journal of pharmacopuncture Vol.10 No.3

Objective This experimental study was performed to investigate the effect of Coptidis rhizoma extract compared with quantity on Staphylococcus aureus that induce keratitis. Methods Minimal inhibitory concentration(MIC) was measured by dropping to $50{\mu}l$ according to density Coptidis rhizoma extract(100%, 10%, 1%, 0.1%) compared with quantity(40g, 80g, 160g). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results 1. MIC on Staphylococcus aureus in Coptidis rhizoma extract was showed anti-bacterial potency compared with quantity and density in 100% and 10% of all samples(40g, 80g, 160g). 2. MIC on Staphylococcus aureus in Coptidis rhizoma extract(40g, 80g, 160g) was showed anti-bacterial potency compared with quantity all samples($20{\mu}l,\;30{\mu}l,\;40{\mu}l,\;50{\mu}l$) in 100% density. 3. MIC on Staphylococcus aureus in Coptidis rhizoma extract(40g, 80g, 160g) was showed anti-bacterial potency compared with quantity all samples ($20{\mu}l,\;30{\mu}l,\;40{\mu}l,\;50{\mu}l$) in 100% density. Anti-bacterial potency of 80g Coptidis rhizoma extract decreased compared with 40g. Anti-bacterial potency of 160g Coptidis rhizoma extract decreased compared with 40g in $20{\mu}l,\;30{\mu}l$. Conclusions Coptidis rhizoma extract was showed anti-bacterial potency compare with quantity and density. In herbal drug, antibacterial potency compare with quantity and density must be studied.

소염 약침액, 황련해독탕, 황련이 Staphylococcus aureus에 미치는 항균효과에 대한 실험적 연구

서형식,Seo, Hyung-Sik 대한약침학회 2006 Journal of pharmacopuncture Vol.9 No.2

Objective : This experimental study was performed to investigate the effect of Soyum Herbal-acupuncture, Hwangryunhaedoktang & Coptidis rhizoma on Staphylococcus aureus that induce keratitis. Methods : MIC was measured by dropping to $50\;{\mu}l$ according to density Soyum Herbal-acupuncture, Hwangryunhaedoktang & Coptidis rhizoma(100%, 10%, 1%, 0.1%). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results : 1. MIC on Staphylococcus aureus in Soyum Herbal-acupuncture disappear all sample. 2. MIC on Staphylococcus aureus in Hwangryunhaedoktang disappear all sample. 3. MIC on Staphylococcus aureus in Coptidis rhizoma was 100%, $50\;{\mu}l$ and anti-bacterial potency increase compare with quantity. Conclusions : The present author think that Soyum Herbal-acupuncture must be extracted a new method for anti-bacterial potency and Coptidis rhizoma was made Herbal-acupuncture for eye drops.

진피산(秦皮散)이 Staphylococcus species(S. aureus, S. epidermis)의 배양일에 따른 항균과의 지속성에 관한 연구

서형식,Seo, Hyeong-Sik 대한약침학회 2007 Journal of pharmacopuncture Vol.10 No.2

Objectives : This experimental study was performed to investigate the continuous anti-bacterial potency of Jinpi-san on cultivation of Staphylococcus species(S. aureus, S. epidermidis) that induce eye disease. Methods : Minimal inhibitory concentration(MIC) was measured by dropping to 50${\mu}$l diluted Jinpi-san(100%, 10%, 1%, 0.1%) on S. aureus, S. epidermidis that were cultivated from 2 to 6 days. Anti-bacterial potency was measured by the size of inhibition zone with change of volume(20${\mu}$l, 30${\mu}$l, 40${\mu}$l, 50${\mu}$l). Results : 1. Anti-bacterial potency of Jinpi-san on S. aureus was appeared in 100% and increased on 6 days. Anti-bacterial potency with change of volume was increased in propotion to increase volume. Anti-bacterial potency of Jinpi-san on S. aureus was appeared continuous. 2. Anti-bacterial potency of Jinpi-san on S. epidermidis was appeared in 100%, 10%, 1% on 2 days and in 100%, 10% on 6 days. In 100% Jinpi-san, Anti-bacterial potency of 6 days was increased, in 10%, Anti-bacterial potency of 2 days was increased, in 1%, Anti-bacterial potency of 6 days was disappear. Anti-bacterial potency with change of volume was increased in propotion to increase volume except for 20${\mu}$l of 6days and increased on 6 days, but bacteria was increased. Anti-bacterial potency of Jinpi-san on S. epidermidis wasn't appeared continuous. Conclusions : Anti-bacterial potency of Jinpi-san on cultivation of S. aureus was showed continuous, but on cultivation of S. epidermidis was not showed continuous.