체내에서 성숙이 재개된 생쥐난자의 투명대 경화

김지수,김해권,박종민,이승재,이준영,김문규,Kim, Ji-Soo,Kim, Hae-Kwon,Park, Jong-Min,Lee, Seung-Jae,Lee, Joon-Young,Kim, Moon-Kyoo 대한생식의학회 1997 Clinical and Experimental Reproductive Medicine Vol.24 No.1

It is well known that the zona pellucidae of mouse oocytes become "hardened" when they are allowed to mature in vitro in the absence of serum components. To see if oocytes already undergone meiotic resumption in vivo exhibit similar zona hardening, hardening of ZP of cumulus-enclosed oocytes(CEOs) was examined after culture in vitro since their release from follicles various hours after hCG injection. When CEOs matured in vivo for 3h or longer were subjected to culture in vitro for 14h with BSA alone, zona hardening was significantly reduced compared to those cultured in vitro from the begining of maturation. However, when CEOs matured in vivo for 5h were freed from cumulus cells and then cultured in vitro with BSA alone, little reduction of zona hardening was observed. Preincubation of CEOs for 5h with fetuin, one of the well known inhibitor of in vitro zone hardening, did not prevent zona hardening during its subsequent culture of CEOs for 14h without fetuin. However, when CEOs precultured with both fetuin and PMSG for 5h and then further cultured with BSA alone for 14h, zona hardening was dramatically reduced. Under these conditions, the expansion of cumulus cell was observed. In addition, CEOs cultured with both BSA and dbcAMP to prevent their meiotic resumption showed a significant increase of zona hardening. Whether the observed zona hardening was correlated with the conversion of ZP2 to $ZP2_{f}$ was examined. Zona pellucida, isolated from CEOs matured for 5h in vivo and then further cultured with BSA alone was subjected to SDS-PAGE. Most of ZP2 molecules from these CEOs did not undergo conversion from ZP2 to $ZP2_{f}$. From these results, it is concluded that CEOs undergone meiotic resumption in vivo do not exhibit zona hardening when they were subsequently cultured in vitro without serum components. It appears that cumulus cells play an important role in this phenomenon.

공공도서관의 VR 기반 교육문화프로그램 개발에 관한 연구

김지수,김성주,박신이,신지원,권선영,Kim, Ji Soo,Kim, Seong Ju,Park, Si Ni,Shin, Ji Won,Kwon, Sun Young 한국정보관리학회 2021 정보관리학회지 Vol.38 No.2

최근 들어 다양한 분야에서 VR 기술을 활용한 콘텐츠가 점차 증가하고 있으며 기술 또한 급속도로 보급되는 추세이다. 이러한 배경에서 공공도서관 또한 시대의 흐름에 따라 VR 기술을 활용한 서비스를 제공할 필요가 있다. 이에 본 연구는 공공도서관의 교육적, 문화적 기능을 고려한 VR 기반 교육문화프로그램을 제안하는 것을 목적으로 한다. 이를 위해 VR 프로그램이 진행되고 있는 국내·외 공공도서관 및 산업군 사례 조사 그리고 선행연구, 이론분석을 통해 적용점 및 시사점을 도출하였으며 이를 종합, 검토하여 공공도서관에서의 VR 기반 교육문화프로그램을 제안하였다. 본 연구에서 제시한 VR 기반 교육문화프로그램은 향후 공공도서관에서 VR 기반 교육문화프로그램 기획을 위한 기초 자료로 활용될 수 있을 것이다. Recently, content using VR technology in various fields is gradually increasing, and the technology is also rapidly spreading. Against this background, public libraries also need to provide services using VR technology according to the flow of the times. Therefore, this study drew application points through the investigation and analysis of domestic and foreign public libraries where VR programs are underway. And based on this, a final proposal is prepared after planning and reviewing VR-based educational and cultural programs in public libraries. VR-based educational and cultural programs introduced can be used as basic data for planning VR-based cultural programs in public library.

생료사물탕(生料四物湯)이 DNCB로 유발된 생쥐의 아토피 피부염에 미치는 영향

김지수,최정화,김종한,정민영,박수연,Kim, Ji-Soo,Choi, Jung-Hwa,Kim, Jong-Han,Jeong, Min-Yeong,Park, Soo-Yeon 대한한방안이비인후피부과학회 2017 한방안이비인후피부과학회지 Vol.30 No.1

Objectives : Atopic Dermatitis (AD) is chronic skin disease characterized by allergic hypersensitivity reactions. Saeng-ryo-Samul-tang (SRSM) can treat skin disease by cooling down blood heat, cealering away congenital fever and detoxicating. This study was conducted to investigate the effects of SRSM on AD induced by 2,4-dinitrochlorobenzene (DNCB) in mice Methods : In this study, the effects of SRSM on changes in body weights, thicknesses of dorsum skin, thicknesses and weights of ear, changes of symptoms on the dorsum skin, histopathological degree of ear and dorsum skin, IL-10 and $TNF-{\alpha}$ in serum were observed. And the effects on the proliferation rates of splenocytes were also investigated in vivo and in vitro study. Results : In SRSM topical application (Topical) group, SRSM oral application (Oral) group and SRSM Combination (Combi) group thickness of dorsum skin decreased significantly. But in TPC, ORL and CBN group, weight of ear didn't show any changes, but thickness of ear decreased significantly. And TPC, ORL and CBN group showed meaningful effectiveness symptoms like desquamation and erythema on AD's clinical espect. In histopathological observation, spongiosis, edema and inflammatory cell infiltration of epidermal were remarkably diminished in TPC, ORL and CBN group. And SRSM diminished the proliferation rates of splenocytes in vivo and vitro study. Conclusions : The present study suggests that SRSM can significantly reduced symptoms of AD, therefore SRSM is effective to treatment of AD.

구안와사에 대한 훈증요법 복합치료 효과

김지수,박수연,최창원,김경수,김경옥,위통순,양승정,Kim, Ji-Soo,Park, Soo-Yeon,Choi, Chang-Won,Kim, Kyeong-Soo,Kim, Kyeong-Ok,Wei, Tung-Shuen,Yang, Seung-Joung 대한한의학방제학회 2015 大韓韓醫學方劑學會誌 Vol.23 No.1

Objectives : This study was performed to investigate the effect of herbal steam therapy on peripheral facial paralysis. Methods : 55 patients with peripheral facial paralysis were treated with Korean Medicine therapy including herbal steam therapy. We evaluated the effect of Korean Medicine therapy including herbal steam therapy by Yanagihara grading system. Results : 1. 55 patients were treated with Korean Medicine therapy and 23.27±14.11 times of herbal steam therapy. 2. After treatment, Y-system score was significantly increased(p-value＜0.001) from 18.00±6.5514 to 35.00±6.3404. Conclusions : Herbal stream therapy seem to be effective to improve symptoms of peripheral facial paralysis. Further studies will be needed to identify the beneficial of herbal steam therapy on peripheral facial paralysis.

한방안이비인후피부과학회지에 게재된 여드름과 관련된 논문에 관한 고찰

김지수,박수연,최정화,김종한,이두희,Kim, Ji-Soo,Park, Soo-Yeon,Choi, Jeong-Hwa,Kim, Jong-Han,Rhee, Doo-Hee 대한한방안이비인후피부과학회 2014 한방안이비인후피부과학회지 Vol.27 No.3

Objective : This study was carried out to analyze the trends of Acne related articles that have been published in the Journal of Korean Medicine Ophthalmology, Otolaryngology & Dermatology(JKOOD). Method : We studied 29 research papers relevant to Acne that published in JKOOD from 1998 to 2013. All the paper were classified into three categories; original article, review article and case report. We sub-classified the original articles into three categories; descriptive study, analytic study and experimental study. We analyzed the case report according to various types of treatment. Results : 1. The number of searched journals is 29 papers; 17 original ordicles, 4 review articles, 8 case reports. 2. Classification of 17 original articles into three categories; 10 experimental studies, 7 descriptive studies, 0 analytic study. 3. Method of Evaluation; Korean Acne Grading System(KAGS) is used frequently in descriptive studies and experimental studies. 4. Treatment in Case reports; 7 Case reports used External medical treatments. Conclusions : It is needed to develop the methods of grading system in acne related to Oriental medicine to obtain objectivity of studies. The number of analytic study, clinical trial and experimental study is need to increase.

희첨 추출물이 B16F10 세포에서 Melanin 생성과 Tyrosinase 활성에 미치는 억제효과

김지수,정민영,김종한,최정화,박수연,Kim, Ji-Soo,Jeong, Min-Young,Kim, Jong-Han,Choi, Jeong-Hwa,Park, Soo-Yeon 대한한방안이비인후피부과학회 2015 한방안이비인후피부과학회지 Vol.28 No.1

Objective : Siegesbeckia Herba can treat various skin disease by expelling wind and removing dampness and clearing away heat and toxic material effects. This study was designed to investigate effects of Siegesbeckia Herba Extracts(SHE) on skin elasticity and whitening using B16F10 cell lines. Method : In this experiment, We observed effect of SHE on cell viability, inhibition of melanin synthesis and inhibitory effect on tyrosinase and elastase. Results : 1. SHE treated group showed decreased cell viability rates significantly compared with non-treated group. More than SHE $250{\mu}g/ml$, $500{\mu}g/ml$ and $1,000{\mu}g/ml$ of treated groups were lower levels of melanin synthesis respectively. 2. SHE significantly showed tyrosinase inhibitory activity in vitro, SHE increased tyrosinase inhibitory activity and elastase inhibitory activity in B16F10 cells, and tyrosinase inhibitory activity in vitro. 3. Tyrosinase inhibitory activity and elastase inhibitory activity in B16F10 cells, tyrosinase inhibitory activity in vitro were not accepted statistical significance compared with non-treated group. 4. SHE treated group showed increased SOD-like activity rates significantly compared with non-treated group. More than SHE $250{\mu}g/ml$, $500{\mu}g/ml$ and $1,000{\mu}g/ml$ of treated groups were lower levels of melanin synthesis respectively. Conclusion : These results suggest that SHE can inhibit melanin synthesis and tyrosinase inhibtory activity. So, We suggest that SHE can be maintained skin whitening.

자연영상 텍스트 이진화를 위한 3단계 색상 군집화 알고리즘

김지수,김수형,Kim Ji-Soo,Kim Soo-Hyung 한국정보처리학회 2005 정보처리학회논문지B Vol.12 No.7

본 논문에서는 자연 영상에서 추출된 텍스트 영상의 이진화를 위한 3단계 색상 분할 알고리즘을 제안한다. 1단계 색상 분할은 입력 영상 중에서 텍스트색상과 배경색상이 잘 구별되는 단순 영상의 이진화를 수행하고, 2단계 색상 분할은 입력 영상에 고주파 필터를 적용하여 자연조명 이나 인공조명에 영향을 받은 영상의 이진화를 수행하며, 3단계 색상 분할은 저주파 필터를 기반으로 텍스트나 배경에 텍스쳐가 존재하는 영상의 이진화를 수행한다. 제안한 이진화 알고리즘이 그레이 정보를 이용하는 이진화 알고리즘보다 효과적으로 텍스트 영역을 이진화함을 입증하기 위해 상용문서 인식기인 아르미 6.0을 사용하여 이진화된 문자영상에 대한 인식 실험을 실행하였다. 그 결과 제안한 알고리즘의 필드 단위 인식률이 그레이 정보를 이용한 이진화 알고리즘의 인식률보다 $35\%$ 이상 우수함을 관측하였다. In this paper, we propose a three-level color clustering algerian for the binarization of text regions extracted from natural scene images. The proposed algorithm consists of three phases of color segmentation. First, the ordinary images in which the texts are well separated from the background, are binarized. Then, in the second phase, the input image is passed through a high pass filter to deal with those affected by natural or artificial light. Finally, the image Is passed through a low pass filter to deal with the texture in texts and/or background. We have shown that the proposed algorithm is more effective used gray-information binarization algorithm. To evaluate the effectiveness of the proposed algorithm we use a commercial OCR software ARMI 6.0 to observe the recognition accuracies on the binarized images. The experimental results on word and character recognition show that the proposed approach is more accurate than conventional methods by over $35\%$.

색상레이어를 이용한 스팸메일 영상에서의 텍스트 영역 추출

김지수,김수형,한승완,남택용,손화정,오성열,Kim Ji-Soo,Kim Soo-Hyung,Han Seung-Wan,Nam Taek-Yong,Son Hwa-Jeong,Oh Sung-Ryul 한국정보처리학회 2006 정보처리학회논문지B Vol.13 No.4

In this paper, we propose an algorithm for extracting text regions from spam-mail images using color layer. The CLTE(color layer-based text extraction) divides the input image into eight planes as color layers. It extracts connected components on the eight images, and then classifies them into text regions and non-text regions based on the component sizes. We also propose an algorithm for recovering damaged text strokes from the extracted text image. In the binary image, there are two types of damaged strokes: (1) middle strokes such as 'ㅣ' or 'ㅡ' are deleted, and (2) the first and/or last strokes such as 'ㅇ' or 'ㅁ' are filled with black pixels. An experiment with 200 spam-mail images shows that the proposed approach is more accurate than conventional methods by over 10%. 본 논문에서는 스팸메일 영상에서 텍스트 영역의 추출을 위한 색상 레이어기반의 알고리즘을 제안한다. CLTE(color layer-based text extraction)는 색상 레이어를 사용하여 영상을 8개로 나눈다. 8개 각각의 영상에서 연결요소를 추출한 후, 연결요소의 크기에 의해서 텍스트 영역과 비텍스트 영역을 분류하고 텍스트 영역을 추출한다. 또한, 추출된 텍스트 영역으로부터 회손된 획 정보를 복구하는 알고리즘을 제안한다. 이진영상내의 한글 문자에는 두 가지 형태의 손상된 획이 존재한다. 첫째 중성 획에 해당하는 'ㅣ' 나 'ㅡ' 등의 획들이 지워지는 경우와, 둘째 초 종성 획에 해당하는 'ㅁ' 이나 'ㅇ'이 흑화소로 채워지는 경우가 있다. 제안한 알고리즘은 이러한 두 가지 손상된 획들을 복구해준다. 200개의 스팸메일 영상을 사용한 실험 결과 제안한 알고리즘이 기존의 텍스트 추출 알고리즘보다 10% 이상 우수함을 관측하였다.

알레르기성 자반증 한방 치험 2례

김지수,최정화,김종한,정민영,박수연,Kim, Ji-Soo,Choi, Jeong-Hwa,Kim, Jong-Han,Jeong, Min-Young,Park, Soo-Yeon 대한한의학방제학회 2016 大韓韓醫學方劑學會誌 Vol.24 No.1

Objective This study is designed to investigate the effect of Korean medical therapy on Allergic purpura patients.Method This is a case report on a male and a female patients who has been suffering from allergic purpura. To reduce her symptoms, we provided with internal herbal medicine 2-3 times a day, acupuncture therapy 1-2 times a day.Results After series of treatments, the symptoms of allergic purpura were remarkably improved.Conclusions This study shows us that Korean medical treatments has remedial values for purpura patients. Hence, more studies should be demanded in Korean medicine for elevation of treatment rate.

말초성 안면신경마비에 대한 매선요법 복합치료 효과

김지수,박수연,김경수,김경옥,위통순,최창원,양승정,Kim, Ji-Soo,Park, Soo-Yeon,Kim, Kyeong-Soo,Kim, Kyeong-Ok,Wei, Tung- Shuen,Choi, Chang-Won,Yang, Seung-Joung 대한한방안이비인후피부과학회 2015 한방안이비인후피부과학회지 Vol.28 No.2

Objective : This study was performed to investigate the effect of Needle-Embedding Therapy on peripheral facial paralysis. Method : We investigated 60 cases of patients with peripheral facial paralysis, and devided patients into two groups : We treated one group by complex korean medical treatment with Needle-Embedding therapy, and did the other group by complex korean medical treatment without Needle-Embedding therapy. Yanagihara grading system at baseline and final were used for evaluating the effect of the treatment. Results : 1. In Needle-Embedding therapy group and non Needle-Embedding therapy group, compared with baseline, at final, Y score was significantly increased.2. At final, there was significant difference in improvement between Needle-Embedding therapy group and non Needle-Embedding therapy group. Conclusions : Needle-Embedding therapy seem to be effective to improve symptoms of peripheral facial paralysis. Further studies will be needed to identify the beneficial of Needle-Embedding therapy on peripheral facial paralysis.