pseudomonas sp. SJ-320로 부터 알칼리성 단백질 가수분해효소의 생산과 특성

김영활 대구보건대학 1995 대구보건대학 論文集 Vol.15 No.-

An alkaline pretense producing microorganism was isolated from soil and identified as Pseudomonas sp. SJ-320. The optimum cultivation condition of Pseudomenas sp. SJ-320 for the production of alkaline pretense was as follow : 1.0% casein 0.2% ammonium chloride, 0.2% NaH₂PO₄, 1.0% fructose, 20t, pH 10.0 and 140h. The optimum pH and temperature for the enzyme activity were pH 8.0 and 50℃, respectively. The enzyme was relatively stable at pH 7.5∼8.5 and at temperature below 50℃. The activity of the partially purified enzyme was inhibited by Hg^(2+) and Cu^(2+) whereas Mn^(2+), Mg^(2+) and Ca^(2+) gave rather activating effects on the enzyme activity. ε-Aminocaproic acid and 4-dinitiophenol did not inhibit the enzyme, but p-chloromercuribenzoic acid and ethytendiaminetetraacetic acid inhibited the enzyme activity, indicating that reactive sulfhydryl group and metal ion group are required for the enzyme activity. The enzyme was resistant to various detergents, except for sodium dodecyl sulfate.

밀폐된 공간에서 에어로빅스가 청각유발전위에 미치는 영향

김영활,박상남 대구보건대학 2009 대구보건대학 論文集 Vol.29 No.-

Aerobicdancing in closedspacecausessomedisorderin thehuman body. Atpresent,therearemanystudiesontheeffectsoftheaerobicdancetothe humanbody,butfew clinicalstudyontheleadersandparticipantsofaerobic dance.In thisstudy,weaimedtogetusefulbasicdatafortheprotection of an auditory disorder,therefore,weselected 30males(testgroup of15males who were acting as leaderofaerobic dancing fora long time,and control group of15maleswhowerenotexperienced in an aerobicdancing and did nothavea neurologicaland otolaryngologicaldisorder).Theaverageageof test group and control group was 34.87±8.80 and 34.07±8.45 year's old respectively,and the average ofthe professionals aerobic dancing in test groupwas8.33±4.73years.Wemeasuredtheauditory evokedpotential(AEP) oftwogroupstreatedwith70㏈ and85㏈ intensityandanalyzedtheabsolute latency(AL)and interpeak latency(IPL)by the SPSS/pc+ 12.0 program.For the comparison ofthe AL between the experimentalgroup and the control groupaccording tointensity,bothearstreatedwith70㏈ showedsignificant difference(p<0.05)intheⅠ,Ⅲ,Ⅴ waveandintheⅠ,Ⅴ waverespectively, andtheexperimentalgroup treatedwith 85㏈ wasshown in theⅠ,Ⅲ,Ⅴ wave(leftear)andintheⅠ wave(rightear)respectively. TheAL and IPL in aerobicdancing.instructordid notshow asignificant differenceintestgroupswithouttheintensity. Inthegroupage20,AL oftheleftearin thetestgrouptreatedwiththe intensity of70㏈ showed asignificantly differencein theⅤ waveand AL oftherightearintheⅠ,Ⅲ,Ⅴ wavesthecontrolgroup.AL oftheleftear in thetestgroup treated with theintensity of85㏈ revealed asignificant differenceintheⅤ waveandAL oftherightearintheⅢ,Ⅴ wavesinthe controlgroup. Inthegroupage30,AL oftheleftearin thetestgrouptreatedwiththe intensity of70㏈ showed significantdifferencein theⅠ,Ⅲ,Ⅴ wavesand AL oftherightearintheⅤ wavethecontrolgroup.Whentreatedwiththe intensity of85 ㏈,the leftand rightearofthe testgroup did notshow significantlydifferenceinthecontrolgroup. In thegroup age40,when treated with theintensity of70㏈ and 85㏈, the rightand lesfear ofthe testgroup did notshow any a significant differencethecontrolgroup. From the above results,we know thatlowersound intensity in the test group showed amoresignificantdifferencethan thecontrolgroup.Forthe professionals aerobic dancing no a significantdifference was revealed.And thenweknow thattestgroup(theleaderofaerobicdancing)exposedtohigh pitched sound alltimethecontrolgroup may havehad morepossibilitiesof developing auditory disorder related with the volume ofa noise and this loudnessmayhavecausedtheotolaryngologicaldisorder.

자동혈구분석기 Coulter Counter GEN-S(USA)로 측정한 백혈구 백분율 참고치

김영활,나영악 대구보건대학 2005 대구보건대학 論文集 Vol.25 No.-

The correlation and reference value between Coulter GEN-S and the manual leukocyte shows as fellows : 1. Reference value of WBC count : 3.5-9.9×10^(3)/㎣ (Male: 4.1-10.5, Female: 3.6-9.2). 2. Correlation for neutrophils for neutrophils(r=0.88), Reference value of neutrophils : 37.2-71.2% (Male: 38.0-71.2, Female: 36.5-71.3). 3. Correlatioin for lymphocytes(r=0.76), Reference value of lymphocytes : 20.5-52.5% (Male: 19.9-51.1, Female: 21.4-53.4). 4. Correlation for monocytes(r=0.52), Reference value of monocytes : 3.2-9.6% (Male: 3.2-10.0, Female: 3.0-9.4). 5. Correlation for eosinophils(r=0.89), Reference value of eosinophils : 0.0-5.9% (Male: 0.0-6.5, Female: 0.0-5.4). 6. Correlation for basophils (r=0.18), Reference value of basophils : 0.0-1.4% (Male: 0.0-1.1, Female:0.0-1.7).

ECG를 이용한 초등학생 집단 심장검진

김영활,김병원 대구보건대학 2002 대구보건대학 論文集 Vol.22 No.-

We know that heart disease is the most common case on the sudden death in children. And so for 6 months in Gyungbuk province since march 1998. we have done mass screening about 1,734 of the elementary school children (male 894, female 840) in first grade using 12-lead ECG, Among 20 children (male 14, female 6) who had an abnormal ECG, pediatric cardiologist examined 18 children to detect heart diseases by 24 hour ECG, echocardiography cardiac catheterization and angiocardiography. In the result, 11.5% of children had abnormal ECG (Male 8.1%, female 3.5%). The most frequent abnormal ECG was incompelete right bundle branch block (ICRBBB) and it was shown the incidence of 5.2 among 1,000 followed by premature ventricular contraction (1.7 among 1,000), right bundle hypertrophy (1.2 among 1,000) and compelete right bundle branch block (1.2 per 1,000). In the echocardiography 3 of 18 had heart disease (2 atrial septal defects, ASD and lventricular septal defect, VSD) and the patients with structural defect (VSD) took open heart surgery with problems. From this results, we thought that 12 lead ECG was one of the useful methods for mass screenung of heart disease, but we could not find the structural defect of heart in the child with abnomal ECG. For the health promotion of the elementary school children with heart disease, we thought that other methods of echocardiogram etc. were necessary.

흡연력에 따른 혈압, 혈액성상 및 혈중 지질패턴의 비교

김영활,황경희 대구보건대학교 2012 대구보건대학 論文集 Vol.32 No.-

The purpose of this study was to compare the blood pressure, blood findings and serum lipid profile according to smoking status. Subjects were recruited from adult men, which included smokers (n=50) and non-smokers (n=50). Anthropometrical measurement, blood pressure measurement and blood collection and analysis were conducted. There was no significant difference in general characteristics(average age, height and weight) among two groups. The RBC count and eosinophil count of the subjects were not significantly different. The hemoglobin, hematocrit, WBC count and neutrophil count of the smokers were significantly higher than those of the non-smokers. Also blood pressure of the smokers were significantly higher than those of the non-smokers. Also blood pressure of the smokers were significantly higher than those of the non-smokers. Serum lipids of the smokers were 198.6±41.7 mg/dL for total cholesterol, 173.4±112.8 mg/dL for triglyceride, 39.6±10.0 mg/dL for HDL-cholesterol, 104.4±29.8 mg/dL for LDL-cholesterol. Especially serum total cholesterol and triglyceride of smokers was significantly higher than those of non-smoker. The above results revealed that blood pressure, hematological findings(hemoglobin, hematocrit, WBC count and neutrophil count) and serum total cholesterol and triglyceride of the smokers were different from non-smokers. Therefore, in order to stop smoking, the effect of smoking on health status should be informed to smokers and more systematic study should be conducted.

콜린에스테라아제 억제제가 국소대뇌 혈류변화에 미치는 영향

김영활,김병원 대구보건대학 2003 대구보건대학 論文集 Vol.23 No.-

To evaluated a regional cerebral blood flow(rCBF) changes and contemporary clinical responses by cholinesterase inhibitor (ChEI), we selected eight probable AD patients (4 males and females 69.63 ages) who were recruited from the dept, of Neurology at Yeungnam University Medical Center, between August 2000 and April 2002. The clinical diagnosis of AD was based on the DSM-Ⅳ and NINCDS-ADRDA criteria. Huchinski ischemic scores of all the patients were below 1. The mean treatment duration was 30.38 weeks ranged from 24 to 44 weeks. Four patients treated with Rivastigmine 12㎎ after titration, and three patients Donepezil 10㎎ during the whole period and only one patient treated with Donepezil 10㎎ after the initial 5㎎ for three weeks. The base line and follow up ^(99m)TC-ECD SPECT studies done within one week before the ChEI treatment and within one week after finishing the study. Regions of interest(ROIs) were drawn over the left and right frontal, temporal, parietal and cerebellum. Region to cerebellar ratio(RCR), each count in the ROIs divided by mean cerebellar ROI count, were calculated as a estimation of rCBF, and base line RCRs were compared with those of the follow up. We found that the most significant increase in blood flow occurred in the bilateral parietal lobes(p<0.05). Reduction in the rCBF is more profound on the left hemisphere in the base line(p<0.05) and it achieved significant increase of rCBF after ChEI treatment compared with right hemisphere(p<0.05).

正常人의 血液化學檢査 結果에 의한 參考値의 推定

김영활 최신의학사 1991 最新醫學 Vol.34 No.4

小兒 肝蛭 患者의 臨床的 特性

김영활,정소웅 대구보건대학 2001 대구보건대학 論文集 Vol.21 No.-

Clinical characteristics of childhood epilepsy was obtained by direct interview or telephone Clinical characteristics of childhood epilepsy was like these : 1. 50% Patient had first seizure under the 5years old, 28% from to 10years old, and 13% over the 11years old. 2. Blood typing study showed 32% at the A-typing, 33% at the B-typing, 20% at the O-typing, and 14% at the AB-typing. 3. 28% Showed high social intro-version(Si) and 47% showed lower Si and 25% nonspecific. 4. 13% Patients showed seizure family history and 87% showed no. 5. The methods of controlling seizure were different : 30% by traditional acupuncture. 27% by herb doctor, 16% by hospital, another 16% by traditional acupuncture with herb doctor, and 11% by others. 6. The religion acticites of epileptic Patient showed that there were 36% at Buddisn, 10% Cathalic, 8% at the Christian and 45% had no any religion.

검사 성적으로부터 정한 전해질 검사 및 신장 검사의 차고치들

김영활 최신의학사 1997 最新醫學 Vol.40 No.3

임상생리학적 검사의 범위와 분야에 대한 고찰

金英豁 대구보건대학 1992 대구보건대학 論文集 Vol.13 No.-

A test dealing with the human body belongs to the science of clinical physiology and test includes Electrocardiogram, Electromyogram, Electroencephalogram, plumonary function, Basal metabolism, Phenocardiogram, Mecanocardiogram etc.