http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
오늘 본 자료
Ultra Real-Time PCR을 활용한 Avian Influenza Virus Subtype의 조기진단법
김상태,김영균,김장수,Kim, Sang-Tae,Kim, Young-Kyoon,Kim, Jang-Su 한국미생물학회 2011 미생물학회지 Vol.47 No.1
조류 인플루엔자 바이러스(AIV) 아형을 ultra-time PCR법(UPCR)을 이용하여 초스피드로 진단할 수 있는 방법을 고안하였다. 표적 대상의 프라이머는 AIV H5N1 아형의 hemagglutinin(HA) 유전자 중 가장 상보성이 높은 133 bp의 부위를 선택하였고, 실험의 안전을 위하여 인공합성의 방법으로 제작하였다. 압타머와 결합한 molecular beacon 기반 Mini-Opticon Q-PCR 기기를 사용한 UPCR법으로, 총 UPCR 반응액의 양을 10 ${\mu}l$으로, UPCR과 용융온도 분석시간을 15분 이내로 매우 짧게 단축시켰다. 민감도 측정에서 최소의 주형인 5분자의 HA 유전자만으로 정확히 AIV의 특이적 133 bp를 합성하였다. UPCR로 디자인된 이 PCR은 AIV 아형의 진단에 적용될 수 있을 뿐 아니라, UPCR이 기반되는 진단을 이용하여 다른 병원체에도 널리 적용 될 수 있을 것으로 기대된다. This ultra real-time PCR (UPCR) based diagnosis system for avian influenza A virus (AIV) subtype was designed. The target primer in this study was derived from H5N1 subtype-specific 133 bp partial gene of hemagglutinin (HA), and was synthesized by using PCR-based gene synthesis on the ground of safety. UPCR was operated by Mini-Opticon Q-PCR Quantitative Thermal Cycler using aptamer-based molecular beacon, total 10 ${\mu}l$ of reaction mixture with extraordinarily short time in each steps in PCR. The detection including UPCR and analysis of melting temperature was totally operated within 15 min. The AIV-specific 133 bp PCR product was correctly amplified until 5 molecules of HA gene as minimum of templates. This kind of PCR was drafted as UPCR in this study and it could be used to detect not only AIV subtype, but also other pathogens using UPCR-based diagnosis.
피로수명예측을 위한 잔류강도 저하모델의 파라미터 결정법 제안(II)
김상태,장성수,Kim, Sang-Tae,Jang, Seong-Su 대한기계학회 2001 大韓機械學會論文集A Vol.25 No.9
A new method of parameter determination in the fatigue residual strength degradation model is proposed. The new method and minimization technique is compared experimentally to account for the effect of tension-compression fatigue loading of spheroidal graphite cast iron and graphite/epoxy laminate. It is shown that the correlation between the experimental results and the theoretical prediction on the fatigue life and residual strength distribution using the proposed method is very reasonable. Therefore, the proposed method is more adjustable in the determination of the parameter than minimization technique for the prediction of the fatigue characteristics.
김상태,Kim, Sang-Tae 한국건강관리협회 1987 건강소식 Vol.11 No.1
직장인의 스트레스와 나아가 이로 인한 정신장애의 극복책은 우선적으로 스트레스의 바른 해소책 개발과 인격적 취약성의 자각 및 인격 성숙을 위한 부단한 노력 이다.
사례기반추론을 이용한 개략비용 예측시스템 개발 - 토양경작법 정화비용사례를 중심으로 적용가능성 검토 -
김상태,심진아,김흥래,Kim, Sang-Tae,Shim, Jin-Ah,Kim, Heung-Rae 한국건설관리학회 2016 한국건설관리학회 논문집 Vol.17 No.1
가중사례기반추론 알고리즘을 이용하여 Excel VBA 기반의 개략비용 예측시스템을 개발하였다. 개발 시스템의 특징은 사례학습과 신규추정시 학습사례건수와 신규추정건수 만큼의 시트를 자동생성하며, 각각의 시트는 셀수식이 자동으로 입력되어 시스템의 활용성을 고려하였으며, 또한 영향인자를 최대 10개까지 선택이 가능하도록 하여 영향인자의 조합을 자유롭게 구성할 수 있도록 하였다. 개발한 비용예측시스템을 토양경작법 정화비용예측에 이용하여 토양정화 예측모델의 적용가능성을 검토하였다. 평균단가 예측모델은 물론 최적다중회귀 예측모델보다 개선된 결과를 확인하였다. 본 연구에서는 토양경작법을 대상으로 검토 하였으나 토양정화기술과 오염물질 종류 등 다양한 시나리오가 나타나는 토양정화사업분야에 사례기반추론을 이용한 비용예측모델은 향후 사례데이터 축적과 더불어 적용가능성이 커질 것으로 기대된다 This study proposes a approximate cost estimation system based on Excel with VBA using weighted CBR(Case Based Reasoning). One characteristic of this system is that it generates the sheet automatically as many as the number of similar case and new estimation when it performs a case learning and a new estimate and cell formula is automatically entered into each sheet. User can be free to compose a combination of attribute factors because they can select up to ten attribute factors. This paper presents an applicability of estimation model for estimating the soil remediation cost when it use a landfarming method. When compared to a estimation model by using average unit cost and optimum multiple regression, this model shows a better result. This study was aimed at landfarming method, but it is expected that a cost estimation model using CBR will be more likely to apply in soil remediation technologies which various remediation technologies and pollutant species exist.
김상태,고우찬,이승우,김흥래,Kim, Sang-Tae,Koh, Woo-Chan,Lee, Seung-Woo,Kim, Heung-Rae 한국지하수토양환경학회 2015 지하수토양환경 Vol.20 No.7
In this study, we have developed the performance evaluation model for the optimal soil remediation technology selection. Performance evaluation model is composed in the evaluation of two steps. In the first stage, the candidate technologies are derived according to the conditions of drilling, type and concentration of pollutants, and the saturated/unsaturated of target site. In the second stage, each individual candidate technology is evaluated by performance evaluation model. The performance evaluation model has 5 groups of evaluation items and 12 evaluation items which have their own evaluation index and their own weights through the AHP approach surveying 40 experts. From the case study of actual design cases, the applicability of the performance evaluation model was confirmed.
흰쥐 정소세포에서의 Cadmium 독성 작용과 Selenium 전처리의 영향
김상태,부문종,최임순 ( Sang Tae Kim,Moon Jong Boo,Rim Soon Choe ) 한국환경생물학회 1990 환경생물 : 환경생물학회지 Vol.8 No.1
To investigate the cadmium toxicity to testis and the effect of selenium pretreatment, male rats were subcutaneously injected with Cd and/or Se prior to Cd. In doing so, the respiration rates of mitochondria, the lysosomal activities of acid phosphatase and the cellular levels of GSH were measured. Also, the testicular ultrastructures were observed. Cd only-treated group showed that the respiration rates of mitochondria, the lysosomal activities of acid phosphatase and the level of GSH were reduced on the whole. In contrast to above group, Se-pretreated group appeared to be recovered: the respiration rate was restored more than that of control group and the enzyme activity was restored somewhat, however less than that of the control group. The level of GSH was recovered to the level of control group in Se-pretreated group. According to ultrastructural studies, Cd-treated group showed the swelling and destruction of mitochondria and the production of lipid droplets. In contrast, Se-pretreated group appeared not to be different from control group. So, all of these results would indicate that Se has a protective function against Cd toxicity in the rat testis.