GC-NPD 동시분석법을 이용한 유니코나졸 잔류실태조사

김은정(Eun-Jeung Kim),최부철(Bu-Chuhl Choi),최수정(Su-Jeong Choi),홍채규(Chae-Kyu Hong),김은희(Eun-Hee Kim),최재만(Chae-Man Choi),서영호(Young-Ho Seo),황영숙,장미라(Mi-Ra Jang),황인숙(In-Sook Hwang),김무상(Moo-Sang Kim),채영주(Young-Zoo 한국식품과학회 2012 한국식품과학회지 Vol.44 No.3

2011년 6월부터 11월까지 농산물 중 GC-NPD 및 GC-MSD를 이용한 동시 다성분 분석법을 이용하여 uniconazole의 잔류실태조사를 실시하였다. 2011년 6월부터 11월까지 서울지역에 유통되고 있는 농산물 121종 총 3,939건에 대하여 잔류실태조사를 실시한 결과 49건에서 uniconazole이 검출되었다. 품목별로는 겨자채가 69.4%로 가장 많이 검출되었다. Uniconazole의 검출양은 0.09 ㎎/㎏에서 17.89 ㎎/㎏로 나타났다. 본 연구에서는 uniconazole에 대하여 GC-NPD 및 GC-MSD를 이용한 동시분석법의 가능성을 검토하였다. GC-NPD의 검출한계와 정량한계는 각각 0.006 ㎎/㎏와 0.018 ㎎/㎏이었다. 겨자채에 표준용액을 0.5, 5 ㎎/㎏ 농도로 하여 회수율을 측정한 결과 83.4%에서 101.4%까지 양호한 결과를 나타내었다. 본 연구를 통하여 동시분석법으로 보다 효율적인 검사가 가능할 것으로 기대된다. The monitoring of the uniconazole residual pesticide for agricultural products was conducted by multiclass pesticide multiresidue methods. Samples were collected from June to November, 2011. Uniconazole pesticide was detected in 49 samples from a total of 3,939 samples. The amount of uniconazole pesticide ranged from 0.09 to 17.89 ㎎/㎏ in 49 samples. This method was described for the simultaneous determination of uniconazole by gas chromatography with a nitrogen phosphorus detector (GC-NPD) and mass spectrometry (MS). The limit of detection and quantification were 0.006 and 0.018 ㎎/㎏ GC-NPD, respectively. For an evaluation of the GC-NPD method, uniconazole spiked into gyeojachae at a level of 0.5, 5 ㎎/㎏ was determined. The recoveries of uniconazole by the GC-NPD method ranged from 83.4 to 101.4%. The results indicate that the method of simultaneous analysis is applicable to uniconazole analysis.

Reply to Park’s Review of Money and Totality

프레드 모슬리 경상대학교 사회과학연구원 2019 마르크스주의 연구 Vol.16 No.2

This short paper is a reply to Hyun Woong Park's review essay of my recent book Money and Totality: A Macro-Monetary of Marx's Logic in Capital and the End of the ‘Transformation Problem’. The paper argues that it is not possible to reconstruct my macro-monetary interpretation of Marx's theory in terms of linear production theory; these two theories are incompatible. It also explains how inputs prices are determined in my macro-monetary interpretation. And, it argues that the assumption that the economy is in long-run equilibrium does not necessarily imply that input and output prices and the rate of profit are determined simultaneously. I very much appreciate Park's substantial review of my book.

Simultaneous determination and identity confirmation of thiodicarb and its degradation product methomyl in animal-derived foodstuffs using high-performance liquid chromatography with fluorescence detection and tandem mass spectrometry

Rahman, Md. Musfiqur,Abd El-Aty, A.M.,Kim, Sung-Woo,Lee, Young-Jun,Na, Tae-Woong,Park, Joon-Seong,Shin, Ho-Chul,Shim, Jae-Han Elsevier 2017 Journal of chromatography. B, Analytical technolog Vol.1040 No.-

<P><B>Abstract</B></P> <P>A high-performance liquid chromatography-fluorescence detection method was developed for the simultaneous determination of thiodicarb and its degradation product methomyl in animal-derived food products, including chicken muscle, beef, pork, table eggs, and milk. Thiodicarb is known to degrade during analysis; therefore, a thorough investigation was carried out, revealing that thiodicarb degrades to methomyl immediately after spiking into a matrix of animal-derived food products. Consequently, thiodicarb was determined as the sum of the parent compound and methomyl. Samples were extracted with acetonitrile and sodium salts, and purified using solid-phase extraction (SPE). The limits of detection (LODs) and quantification (LOQs) were 0.0013 and 0.004mg/kg, respectively, for both analytes in various matrices. Seven-point external calibration curves were obtained, and they showed excellent linearity with determination coefficients (<I>R<SUP>2</SUP> </I>)≥0.999 for all tested matrices. The method was validated at three fortification levels (LOQ, LOQ×2, and LOQ×10) in triplicate with average recoveries ranging from 84.24 to 112.8% (for methomyl) and relative standard deviations (RSDs)≤6.5% in all matrices. The converted recoveries of thiodicarb in various matrices ranged from 74.80 to 107.80% with RSDs≤4.5%. The identities of both compounds in standard solutions and for recovery were confirmed using liquid chromatography-tandem mass spectrometry (LC–MS/MS). The developed method was further validated by accurate reproduction at another laboratory. Finally, the method was applied to market samples collected from different areas (and, in the case of milk, different brands), and none of the samples tested positive for thiodicarb or methomyl. In conclusion, the developed method can be successfully applied for a single-run analysis of thiodicarb and methomyl in livestock products.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Due to the frequent degradation of thiodicarb in animal food products, its recovery was very poor. </LI> <LI> It was observed that thiodicarb was breakdown to methomyl just after fortification. </LI> <LI> Improvement or development of a methodology cannot improve thiodicarb recovery. </LI> <LI> At last, a simultaneous method was developed after consideration of methomyl and thiodicarb as a total residue of thiodicarb. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

가압경수로형 원전에서 바생된 폐수지의 14C 및 3H 동시 분리 측정

박순달,김종구,김정석,한선호,지광용 한국방사성폐기물학회 2007 방사성폐기물학회지 Vol.5 No.3

Simultaneous Determination of Anions and Cations in Natural Water by Ion-exclusion/Cation-exchange Chromatography with a Weakly Acidic Cation-exchange Resin Column

Lee, Kwang-Pill,Choi, Seong-Ho,Park, Yu-Chul,Bae, Zun-Ung,Lee, Mu-Sang,Lee, Sang-Hak,Chang, Hye-Yong,Kwon, Se-Mok,Kazuhiko Tanaka Korean Chemical Society 2003 Bulletin of the Korean Chemical Society Vol.24 No.9

The simultaneous determination of anions ($SO_4 ^{2-},\;Cl^-,\;and\;NO_3^-$) and cations ($Na^+,\;NH^{4+},\;K^+,\;Mg^{2+},\;and\;Ca^{2+}$) in natural water obtained by Nakdong River waters system in Korea were performed by ion-exclusion/cationexchange chromatography with conductimetric detection. The stationary phase was a polymethacrylate-based weakly acidic cation-exchange resin column in the $H^+$-form and a weak-acid eluent. When using only a 1.4 mM sulfosalicylic acid/6 mM 18-crown-6 ether as an eluent, good resolution of both anions and cations, minimum time required for the separation, and satisfactory detection sensitivity were obtained in a reasonable time. The method was successfully applied to the simultaneous determination of anions and cations in natural waters.

Simultaneous Spectrometric Determination of Caffeic Acid, Gallic Acid, and Quercetin in Some Aromatic Herbs, Using Chemometric Tools

Kachbi, Abdelmalek,Abdelfettah-Kara, Dalila,Benamor, Mohamed,Senhadji-Kebiche, Ounissa Korean Chemical Society 2021 대한화학회지 Vol.65 No.4

The purpose of this work is the development of a method for an effective, less expensive, rapid, and simultaneous determination of three phenolic compounds (caffeic acid, gallic acid, and quercetin) widely present in food resources and known for their antioxidant powers. The method relies on partial least squares (PLS) calibration of UV-visible spectroscopic data. This model was applied to simultaneously determine, the concentrations of caffeic acid (CA), gallic acid (GA), and quercetin (Q) in six herb infusion extracts: basil, chive, laurel, mint, parsley, and thyme. A wavelength range (250-400) nm, and an experimental calibration matrix with 21 samples of ternary mixtures composed of CA (6.0-21.0 mg/L), GA (10.0-35.2 mg/L), and Q (6.4-17.5 mg/L) were chosen. Spectroscopic data were mean-centered before calibration. Two latent variables were determined using the contiguous block cross-validation procedure after calculating the root mean square error cross-validation RMSECV. Other statistic parameters: RMSEP, R², and Recovery (%) were used to determine the predictive ability of the model. The results obtained demonstrated that UV-visible spectrometry and PLS regression were successfully applied to simultaneously quantify the three phenolic compounds in synthetic ternary mixtures. Moreover, the concentrations of CA, GA and Q in herb infusion extracts were easily predicted and found to be 3.918-18.055, 9.014-23.825, and 9.040-13.350 mg/g of dry sample, respectively.

Simultaneous Determination of Eight Marker Compounds in the Traditional Herbal Medicine, Sipjundaebo-tang by HPLC-DAD

Hye Jin Yang,마충제,마진열,Jin Bae Weon 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.9

Sipjundaebo-tang, known as a traditional herbal medicine, has been used in the treatment of anemia, inflammation, and tumor. For simultaneous determination of eight components, namely 5-HMF, paeoniflorin, ferulic acid, cinnamaldehyde, decursinol, 6-gingerol, decursin, and glycyrrhizin in Sipjundaebo-tang, a high performance liquid chromatography-diode array detector (HPLC-DAD) method was established. In order to develop and validate this HPLC-DAD method, C_18 column (S-5 μm, 4.6 × 250 mm) was used with gradient mobile phase at the column temperature of 35^oC. The mobile phase was consisted of water and methanol containing 0.1% trifluoroacetic acid. UV wavelength was set at 230, 254, 280, and 300 nm. Validation of the analytical method was evaluated by linearity, precision, and accuracy test. Calibration curve for eight marker compounds showed good linearity with R^2 > 0.9994. Limits of detection and Limits of quantification ranged from 0.01 μg/mL to 0.13 μg/mL and 0.03 μg/mL to 0.41 μg/mL, respectively. The relative standard deviations (RSDs) value of precision test, intra-day and inter-day test, was less than 1.14% and 2.54%, respectively. The results of accuracy test were varied from 98.31% to 104.88% with RSD < 2%. This developed simultaneous determination method was efficient to the quality control of Sipjundaebo-tang.

HPLC-DAD를 이용한 귀비탕 중 6종 생리활성 물질의 동시분석법 확립

양혜진,원진배,마진열,마충제 한국생약학회 2010 생약학회지 Vol.41 No.4

Guibi-tang, a traditional herbal medicine, is used for anti-oxidant, anti-osteoporosis, hemostasis and gastroprotection. To develop an analysis method of simultaneous determination of six compounds, swertisin, decursinol, glycyrrhizin,6-gingerol, costunolide and decursin in Guibi-tang, a high performance liquid chromatography was used with diode array detector. Six bioactive components were separated on a SHISEIDO C18 column (5 µm, 4.6 mm I.D.×250 mm) with column temperature 30oC. The gradient elution was composed of water with 0.1% trifluoroacetic acid (TFA) and acetonitrile. UV wavelength was set at 230 nm, 254 nm and 330 nm, respectively. Calibration curve showed good linear regression (R 2>0.9999). The limits of detection (LOD) and the limits of quantification (LOQ) ranged in 0.03 - 0.23 µg/ml and 0.08 - 0.70 µg/ml, respectively. The RSD values of intra- and inter-day test were in the range of 0.03 ~ 0.96% and 0.01 ~ 1.46%, respectively. The evaluated results of accuracy test were varied from 92.28% ~ 105.14% with RSD < 1.60%. In conclusion, this developed simultaneous determination method was accuracy and sensitive to the quality evaluation of Guibi-tang.

Simultaneous Determination of Cinnamaldehyde and Coumarin in Oryeong-san using HPLC with Photodiode Array Detector

서창섭,신현규 대한한의학방제학회 2010 大韓韓醫學方劑學會誌 Vol.18 No.2

Objectives : To develop and validate High-performance liquid chromatography-photodiode array methods for simultaneous determination of two constituents in Oryeong-san(ORS). Methods : Reverse-phase chromatography using a Gemini C18 column operating at 40°C, and photodiode array(PDA) detection at 280 nm, were used for quantification of the two marker components of ORS. The mobile phase using a gradient flow consisted of two solvent systems. Solvent A was H2O and solvent B was acetonitrile. Results : Calibration curves were acquired with correlation coefficient (r2)>0.9999, and the relative standard deviation(RSD) values(%) for intra- and inter-day precision were not exceed 1.0%. The recovery rate of each compound was in the range of 93.01-104.16%, with an RSD less than 2.0%. The contents of two compounds in ORS were 1.10-3.72 mg/g. Conclusions : The established HPLC method will be helpful to improve quality control of ORS. Objectives : To develop and validate High-performance liquid chromatography-photodiode array methods for simultaneous determination of two constituents in Oryeong-san(ORS). Methods : Reverse-phase chromatography using a Gemini C18 column operating at 40°C, and photodiode array(PDA) detection at 280 nm, were used for quantification of the two marker components of ORS. The mobile phase using a gradient flow consisted of two solvent systems. Solvent A was H2O and solvent B was acetonitrile. Results : Calibration curves were acquired with correlation coefficient (r2)>0.9999, and the relative standard deviation(RSD) values(%) for intra- and inter-day precision were not exceed 1.0%. The recovery rate of each compound was in the range of 93.01-104.16%, with an RSD less than 2.0%. The contents of two compounds in ORS were 1.10-3.72 mg/g. Conclusions : The established HPLC method will be helpful to improve quality control of ORS.

Simultaneous Determination of Seven Compounds in Samsoeum by HPLC-PDA

서창섭,김정훈,황대선,신현규 대한한의학방제학회 2010 大韓韓醫學方劑學會誌 Vol.18 No.1

A HPLC method was developed for simultaneous determination of puerarin, daidzin, liquiritin, naringin, hesperidin, neohesperidin, and glycyrrhizin, to evaluate the quality of SSE. In the present work, simultaneous determination of the seven marker compounds in SSE was validated with respect to linearity, precision, and accuracy. The method will be helpful to improve quality control of SSE.