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영지(Ganoderma lucidum) 균사체의 액체배양에 의한 세포외 생물고분자의 생산조건과 특성
이신영,강태수 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1
새로운 생물산업소재의 탐색 및 개발연구의 일환으로 Ganoderma lucidum(영지1호) 균사체의 액체배양에 의하여 세포외 생물고분자의 생산을 위한 최적 생산조건을 검토하였고, 생성 생물고분자를 분획 정제하여 이의 성분특성을 조사하였다. 세포의 생물고분자 생산을 위한 최적 배양조건은 glucose 5%, yeast extract 0.5%, (NH_4)_2HPO_4 0.1% 및 KH_2PO_4 0.05%(w/v)를 함유한 배지에서 pH 5.0, 온도 30℃ 및 교반속도 100 rpm일 때 얻어졌다. 이들 조건하에서 7일간 플라스크 배양하였을 때, 최대의 균체 및 세포외 생물고분자 농도는 각각 15.2 및 18.8 g/l이었으며, 비생육속도, 비기질소비속도 및 비생산속도는 각각 0.039 hr^-1, 0.043 gg ^-1hr^-1이었다. 세포의 생물고분자는 실온의 물추출에 의하여 수용성 및 물불용성 다당류로 분획되었으며, 두 시료 모두 97% 이상의 당을 함유하였다. 수용성 생물고분자 분획(BWS)과 물불용성 분획(BWI)은 모두 glucose, galactose, mannose, xylose 및 fucose를 함유하였으며, 구성당의 몰비는 각각 3.6 : 1.5 : 2.1 : 0.5: trace 및 2.9 : 3.1 : 2.0 : 1.6 : 0.3이었다. For the screening and the development of the new bio-material, cultural conditions for the exo-biopolymer (EBP) production through the submerged cultivation of Ganoderma lucidum mycelium were investigated. Also, the fractionations and the purifications of the exo-biopolymer were carried out and the chemical compositions of the exo-biopolymer were examined. The optimal culture conditions for the exo-biopolymer production were pH 5.0, 30℃ and 100 rpm of agitation speed in the medium containing of 5% (w/v) glucose, 0.5% (w/v) yeast extract, 0.1% (w/v) (NH_4_)_2HPO_4, and 0.05% (w/v) KH_2PO_4. In the flask cultivation for 7 days under these conditions, the concentration of the maximum exo-biopolymer and the cell mass were 15.4 g/l and 18.8 g/l, respectively. The specific growth rate was 0.039 hr^-1. In addition, the substrate consumption rate, and the exo-biopolymer production rate were 0.043 gg ^-1hr^-1 and 0.025 gg ^-1hr^-1, respectively. The exo-biopolymer was fractionated into BWS (water soluble exo-biopolymer) and BWI (war insoluble exo-biopolymer) by the water insoluble exo-biopolymer) by the water extraction, and the sugar contents fo two fractions were higher than 97% (based on dry basis). The components sugar of BWS and BWI fractions were glucose, galactose, mannose, xylose, and fucose. Their molar ratios were 3.6:1.5:2.1:0.5: trace and 2.9:3.1:2.0:1.6:0.3, respectively.
Optimization of Submerged Culture Conditions for Exo-Biopolymer Production by Paecilomyces japonica
BAE, JUN TAE,SINHA, JAYANTA,PARK, JONG PIL,SONG, CHI HYUN,YUN, JONG WON 한국미생물 · 생명공학회 2000 Journal of microbiology and biotechnology Vol.10 No.4
Optimization of submerged culture conditions for the production of exo-biopolymer from Paecilomyces japonica was studied. Maltose, yeast extract, and potassium phosphate were the most suitable sources of carbon, nitrogen, and inorganic salt, respectively, for both production of the exo-biopolymer and mycelial growth. The optimal culture conditions in a flask culture were pH 5.0, 25℃, and 150rpm in a medium containing (as in g/l) 30 maltose. 6 yeast extract, 2 polypeptone, 0.5 K_2HPO_4, 0.2 KH_2PO_4, 0.2MnSO_4·5H_2O, 0.2MgSO_4·7H_2O. Exo-biopolymer production and mycelial growth in the above suggested medium were significantly increased in a 2.5-1 jar fermentor, where the maximum biopolymer concentration was 8g/l. The morphological changes of the mycelium in the submerged culture were observed within pH ranges from 4.0 to 9.0; i.e., growth of the filamentous form was optimal at culture pHs of 5.0 and 6.0, whereas pellet was formed at other pHs.
발효기 종류에 따른 Cordyceps militaris에 의한 생물고분자 생산
이중헌,김경주 조선대학교 생산기술연구소 2005 生産技術硏究 Vol.27 No.1
The effects of fermenter types on biopolymer production with Cordyceps militaris has been studied in this articles. Since the shear stresses of stirred tank ferementor and bubble column fermenter were different. the mycelium growth and biopolymer production were different, The mycerial growth was high with the bubble column fermenter for the early stage but biopolymer production was lower than that with stirred tank fermenter. The production of biopolymer was closely related to shear stress to the cells and biopolymer production was increased with increased shear stress.