Chromium Trioxide의 독성에 대한 산수유 추출물의 항산화 효과

서영미,Seo, Young-Mi 대한임상검사과학회 2018 대한임상검사과학회지(KJCLS) Vol.50 No.2

본 연구 목적은 산수유 추출물의 항산화 효과를 배양 C6 glioma 세포를 대상으로 조사하였다. 이 같은 목적을 위하여 C6 glioma 세포를 48시간 동안 배양한 후 6가 크롬의 세포독성 및 산수유 추출물의 방어효과를 조사하였다. 이외에 EDA 및 LDH 활성과 같은 항산화 효과를 분석하였다. 본 연구에서 $CrO_3$는 처리농도에 비례하여 세포생존율을 감소시켰다. 또한 이 과정에서 세포를 48시간 동안 20~40 uM로 각각 포함된 배양액에서 처리한 결과 $XTT_{50}$ 값은 33 uM로 나타났다. 항산화제인 CAT는 $CrO_3$로 유도된 세포독성에 의해 감소된 세포생존율을 유의하게 증가시켰다. 한편 산수유 추출물의 보호효과에 있어서, 산수유 추출물은 $CrO_3$만의 처리군에 비하여 세포생존율을 유의하게 증가시켰다. 동시에 산수유 추출물은 EDA와 LDH 활성과 같은 항산화 효과를 보였다. 이와 같은 결과로부터 $CrO_3$의 독성에 산화적 손상이 관련되어 있는 것으로 나타났다. 또한 산수유 추출물은 이의 항산화 효과에 의하여 $CrO_3$의 세포독성을 효과적으로 방어하였다. 결론적으로, 산수유 추출물과 같은 천연소재는 산화적 손상과 관련된 중금속에 의해 유발된 세포독성을 방어 내지는 치료하는데 유용한 치료적 요소의 하나로 생각된다. This study examined the antioxidative effects of Cornis fructus (CF) extract on cultured C6 glioma cells. For this purpose, cytotoxicity analysis of chromium trioxide ($CrO_3$) was performed and the protective effects of the CF extract on $CrO_3$-induced cytotoxicity was examined after the C6 glioma cells were cultured for 48 hours. The antioxidative effects, such as electron donating activity (EDA) and lactate dehydrogenase (LDH) activity were also analyzed. In this study, $CrO_3$ decreased the cell viability in a dose dependent manner. The $XTT_{50}$ value was determined to be $33{\mu}M$ after the cells were treated for 48 hours at a concentrations of $20{\sim}40{\mu}M$ $CrO_3$. The catalase (CAT) antioxidant increased significantly the cell viability that had been decreased by $CrO_3$-induced cytotoxicity. Regarding the protective effect of the CF extract, the cell viability of the CF extract was increased significantly compared to that of $CrO_3$ only. In addition, the CF extract showed antioxidative effects, such as EDA and an inhibitory effect on the LDH activity. These findings suggest that the cytotoxicity of $CrO_3$ is correlated with oxidative stress, and the CF extract effectively prevented $CrO_3$-induced cytotoxicity through the antioxidative effects. In conclusion, natural products, such as the CF extract may be a useful therapeutic agent for the prevention or treatment of toxicity induced by heavy metals via oxidative stress.

계피 추출물의 항산화 효과 및 화장품소재의 응용

이영숙,유민정 한국피부과학연구원 2019 아시안뷰티화장품학술지 Vol.17 No.1

Purpose: This study examines potential abilities and commercial values of Cinnamon extract as bioactive and cosmetics ingredients. Methods: Cinnamomum cassia bark was extracted with hot water and 70% ethanol to examine its antioxidant effects through its total polyphenol, flavonoid content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, and (2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) ABTS radical scavenging activity. Also, this study examined its nitric oxide (NO) inhibition effect through RAW 264.7 cells into which inflammatory reaction was induced by lipopolysaccharides (LPS), and antihistamine activity in the RBL-2H3 cells. The antibacterial effects of Cinnamomum cassia bark extract on 8 types of bacteria were also studied. Results: As a result of measuring the antioxidant effect, the total polyphenol contents were 90.12 and 113.07 μg/mL, respectively, while the total flavonoid contents were 36.42 and 54.31 μg/mL, respectively. The DPPH scavenging effect was confirmed as 84.93% in the hot water extract and 90.25% in the ethanol extract for 400 μg/mL. The ABTS radical scavenging effect was 82.20% in the hot water extract and 92.21% in the ethanol extract for 400 μg/ mL. Upon measuring the NO inhibitive effect through the RAW 264.7 cells into which inflammation reaction was induced by LPS, the NO generation decreased to 14.57 μM in the hot water extract and 10.15 μM in the ethanol extract in the concentration of 100 μg/mL. This result, compared to the increase up to 20.11 μM by LPS, shows the NO inhibitive effect of Cinnamoum cassia bark extract. As a result of measuring the antihistamine activity in the RBL-2H3 cells, β-hexosaminidase increased to 176.21% by IgE-NDP whereas it was 117.25% in the hot water extract and 100.09% in the ethanol extract in the concentration of 100 μg/mL, confirming the inhibitive effect on β-hexosaminidase discharge. The antibacterial effect was confirmed on all bacteria through the measurement on the Cinnamomum cassia Bark extract on eight types of bacteria. Particularly, the antibacterial effect of the hot water extract and ethanol extract was found to be high in Staphylococcus epidermidis (S. epidermidis), Pityrosporum ovale (P. ovale). Conclusion: Cinnamomum cassia bark extract is deemed prospective as a natural functional cosmetics’ ingredient with excellent antioxidant, anti-inflammatory, antihistamine inhibitive and antibacterial effects. 목적: 본 연구에서는 계피 추출물의 생리활성 및 화장품 소재로서의 가능성과 산업적 활용가치를 확인하고자 하였다. 방법: 계피를 열수 그리고 70% 에탄올로 추출물하여 이용하여 총 폴리페놀, 플라보노이드 함량, 2,2-Diphenyl-1-picrylhydrazyl (DPPH) 소거활성, ABTS radical 소거활성을 조사하여 항산화 효과를 확인하였으며, lipopolysaccharides (LPS)로 염증반응이 유도된 RAW 264.7 세포를 통해 nitric oxide (NO) 생성 저해 효과와 RBL-2H3세포에서 항히스타민 활성을 알아보았다. 그리고 8종의 균에 대한 계피 추출물의 항균효과도 확인 하였다. 결과: 항산화 효과 측정 결과, 총 폴리페놀 함량은 각각 90.12, 113.07 μg/g 이고, 총 플라보노이드 함량은 36.42, 54.31 μg/g의 함량이 확인 되었다. DPPH 소거활성은 400 μg/mL에서 열수 추출물은 84.93%, 에탄올 추출물은 90.25%의 소거능이 확인 되었으며, ABTS 라디칼 소거활성을 측정 결과, 400 μg/mL에서 열수 추출물은 82.20%, 에탄올 추출물은 92.21%의 소거능이 확인 되었다. LPS로 염증반응이 유도된 RAW 264.7 세포를 통해 NO 저해 효과 측정 결과, LPS에 의해 NO의 생성량이 20.11 μM까지 증가된 것에 비해 100 μg/mL의 농도에서는 열수 추출물은 14.57 μM 에탄올 추출물은 10.15 μM 까지 감소된 것으로 보아 계피 추출물의 NO 생성 억제 효과를 확인할 수 있었다. RBL-2H3 세포에서 항히스타민 활성을 측정한 결과, IgE-DNP에 의해 β-hexosaminidased이 176.21%로 증가된 것에 비해 100 μg/mL의 농도에서는 열수 추출물은 117.25% 에탄올 추출물은 100.09%로 β-hexosaminidase 방출에 대한 억제 효과를 확인할 수 있었다. 8종 균에 대한 계피 추출물의 추출물의 항균효과를 측정한 결과 모든 균에서 항균효과가 확인 되었으며 특히 S. epidermidis, P. ovale 에서 열수 추출물과 에탄올 추출물 항균효과가 높게 확인되었다. 결론: 계피 추출물은 항산화 효과 및 항염 효과 및 항히스타민 억제효과 그리고 항균효과가 우수하였으 며, 천연 기능성 화장품 소재로서의 가능성이 있다고 판단된다.

보문 : 염모제 성분인 FeSO4의 세포독성에 대한 산사 추출물의 항산화 효과

정재윤 ( Jai Yun Jung ),서영미 ( Young Mi Seo ),정인주 ( In Ju Jung ) 대한미용학회(구 대한미용과학회) 2013 대한미용학회지 Vol.9 No.4

To clarify the antioxidative effect of Crataegi Fructus (CF) extract on the cytotoxicity induced by FeSO4, hair dye component, antioxidative effects such as DPPH-radical scavenging activity and lactate dehydrogenase (LDH) activity as well as cell viability by XTT assay were performed. Cell viability and LDH activity were assessed after NIH3T3 fibroblasts were cultured in media containing various concentrations of FeSO4. And also, the effect of antioxidant, vitamin E on FeSO4-induced cytotoxicity was evaluated. For the protective effect of CF extract on FeSO4-induced cytotoxicity, cell viability was measured after NIH3T3 fibroblasts were pretreated with 130 or 160 μg/mL of CF extract for 2 hours, and also, DPPH-radical scavenging activity and the inhibitory activity of LDH were assessed on CF extract. In this study, FeSO4 significantly decreased cell viability in dose-dependently compared with control, and XTT50 value was calculated at 33.4 μM of FeSO4. In the protective effect of vitamin E, it significantly increased cell viability damaged by FeSO4-induced cytotoxicity. In the protective effect of CF extract on FeSO4-induced cytotoxicity, CF extract significantly increased cell viability which was decreased by FeSO4, and also it showed DPPH-radical scavenging activity and the inhibitory effect of LDH activity. From these results, it is suggested that the oxidative stress is involved in the cytotoxicity of FeSO4, and also, CF extract effectively prevented FeSO4-induced cytotoxicity by antioxidative effect. Conclusively, the natural plant extract such as CF may be a putative resources for beauty by the prevention or diminution of the cytotoxicity of hair dye component such as FeSO4 correlated with oxidative stress.

염모제 성분인 FeSO4의 세포독성에 대한 산사 추출물의 항산화 효과

정재윤,정인주,서영미 대한미용학회 2013 대한미용학회지 Vol.9 No.4

To clarify the antioxidative effect of Crataegi Fructus (CF) extract on the cytotoxicity induced by FeSO4, hair dye component,antioxidative effects such as DPPH-radical scavenging activity and lactate dehydrogenase (LDH) activity as well as cell viability by XTT assay were performed. Cell viability and LDH activity were assessed after NIH3T3 fibroblasts were cultured in media containing various concentrations of FeSO4. And also, the effect of antioxidant, vitamin E on FeSO4-induced cytotoxicity was evaluated. For the protective effect of CF extract on FeSO4-induced cytotoxicity, cell viability was measured after NIH3T3 fibroblasts were pretreated with 130 or 160 μg/mL of CF extract for 2 hours, and also,DPPH-radical scavenging activity and the inhibitory activity of LDH were assessed on CF extract. In this study, FeSO4significantly decreased cell viability in dose-dependently compared with control, and XTT50 value was calculated at 33.4μM of FeSO4. In the protective effect of vitamin E, it significantly increased cell viability damaged by FeSO4-induced cytotoxicity. In the protective effect of CF extract on FeSO4-induced cytotoxicity, CF extract significantly increased cell viability which was decreased by FeSO4, and also it showed DPPH-radical scavenging activity and the inhibitory effect of LDH activity. From these results, it is suggested that the oxidative stress is involved in the cytotoxicity of FeSO4, and also, CF extract effectively prevented FeSO4-induced cytotoxicity by antioxidative effect. Conclusively, the natural plant extract such as CF may be a putative resources for beauty by the prevention or diminution of the cytotoxicity of hair dye component such as FeSO4 correlated with oxidative stress. 장미과(Rosaceae)에 속하는 산사(Crataegi Fructus, CF) 추출물이 염모제 성분인 FeSO4에 미치는 영향을 조사하기 위하여 NIH3T3 섬유모세포를 배양한 후 FeSO4의 세포독성과 이에 대한 산사 추출물의 영향을 항산화 측면에서 조사하였다. 본 연구에서 배양 NIH3T3 섬유모세포에 FeSO4를 처리한 결과 세포생존율을 유의하게 감소시켰으며, XTT50값이 33.4μM에서 나타났다. 한편, 항산화제인 vitamin E는 FeSO4의 세포독성에 의하여 감소된 세포생존율을 유의하게 증가시킴으로서 FeSO4의 세포독성을 방어하였다. 한편, 산사 추출물이 FeSO4의 세포독성에 미치는 영향의 조사에 있어서, 산사 추출물은 FeSO4만의 처리에 비하여 세포생존율을 유의하게 증가시켰다(p<0.01). 이와 동시에, 산사 추출물은 DPPH-라디칼 소거능과 lactate dehydrogenase(LDH) 활성을 억제함으로서 항산화능을 보였다. 이상의 결과로부터 FeSO4의 세포독성에 산화적 손상이 관여하고 있으며, 또한 산사 추출물은 항산화에 의하여 염모제 성분인 FeSO4의 세포독성을 효과적으로 방어하였음을 제시하였다. 따라서, 산사 추출물과 같은 천연물질을 대상으로 FeSO4와 같이 산화적 손상과 관련된 미용제 성분의 독성을 항산화 측면에서 방어 내지는 경감할 수 있는 미용소재로서의 개발적 가치가 크다고 생각된다.

멜라닌생성 자동산화제인 FeSO4의 세포독성 및 멜라닌화에 대한 청미래덩굴 추출물의 영향

표애자,윤미영,양현옥 한국피부과학연구원 2013 대한피부미용학회지 Vol.11 No.1

To evaluate the cytotoxicity and protective effect of Smilax china L. (SC) extract on ferrous sulfate (FeSO4), an autooxidant of melanin formation, cell viability were analysed by XTT assay after human skin melanoma cells (SK-MEL-3) were cultured in media containing various concentrations of FeSO4. And also, the effect of antioxidant α-tocopherol on FeSO4-induced cytotoxicity was assessed. For the protective effect of SC extract on FeSO4-induced cytotoxicity, SK-MEL-3 cells were pretreated with 50 or 80 μg/㎖ of SC extract for 2 h before the treatment of FeSO4. And also, the antioxidative effects of SC extract were assessed by latate dehydrogenase (LDH) activity. In this study, FeSO4 remarkably decreased cell viability dose-dependent manner compared with control. And the XTT50 value was determined at 62.0 μM of FeSO4. In the effect of antioxidant, α-tocopherol effectively prevented FeSO4-induced cytotoxicity by the significant increase of cell viability. In the protective effect of SC extract on FeSO4-induced cytotoxicity, SC extract remarkably increased cell viability which was decreased by FeSO4-induced cytotoxicity, and also it showed the antioxidative effects such as a significant decrease of LDH activity. In the melanin generation, SC extract effectively blocked melanin generation by the decrease of tyrosinase activity and total amount of melanin. From these results, it is suggested that the cytotoxicity of FeSO4 was involved in oxidative stress, and also, SC extract effectively prevented the cytotoxicity and melanogenesis induced by an autooxidant of melanin formation, FeSO4 through antioxidative effect. Conclusively, SC extract may be a putative resources as an protective agent for oxidative stress-mediated skin hyperpigmentation via hyperactivity of autooxidant in melanin formation.

수세미오이(Sponge-gourd: Luffa cylindrica L.) 추출물의 항산화, 항돌연변이 및 암세포 증식 억제 효과

오세인,이미숙,Oh, Se-In,Lee, Mee-Sook 한국식품영양학회 2012 韓國食品營養學會誌 Vol.25 No.4

Considering the dearth of information regarding the medicinal properties of Luffa cylindrica, we assessed the antioxidative, antimutagenic and hyperplasia inhibitory activity of cancer cells from Luffa cylindrica extracts by employing biological and biochemical assays. Ethanol extracts of Luffa cylindrica inhibited MDA-BSA (malondialdehyde-bovine serum albumin) conjugation reaction ($66.38{\pm}2.65$), DPPH (1,1-diphenyl-2-picryl-hydrazyl) radical production ($60.13{\pm}0.42$) and lipid peroxidation ($56.04{\pm}3.24$). In this study, Luffa cylindrica is believed to exert possible antioxidative effects. The direct and indirect antimutagenic effects of the ethanol extracts of Luffa cylindrica were examined by the Ames test using Salmonella typimurium TA98 and TA100. The inhibitory effects on indirect and direct mutagenicity shows an weak tendency, particularly in direct mutagenicity mediated by 2-nitrofluorene in Salmonella typimurium TA98 ($5.82{\pm}5.74$) and in indirect mutagenicity mediated by 2-anthramine in Salmonella typimurium TA100 ($5.76{\pm}2.15$). The ethanol extracts of Luffa cylindrica on cancer cell hyperplasia inhibitory activity via MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay exerted cytotoxic effects on Hela cells ($55.83{\pm}3.83$) and MCF-7 cells ($33.03{\pm}2.09$), which were used in this study. Based on these results, it believed that the ethanol extracts of Luffa cylindrica have antioxidative capacities as well as hyperplasia inhibitory activity of cancer cells. Furthemore, Luffa cylindrica is a candidate for the prevention and dietetic treatment of chronic diseases and for the development of functional food.

차씨오일의 항산화 활성 및 뇌 해마유래 HT22 세포에서의 산화적 스트레스에 대한 보호효과

김종민(Jong Min Kim),박선경(Seon Kyeong Park),강진용(Jin Yong Kang),배성경(Seong-kyeong Bae),정가희(Ga-Hee Jeong),조경환(Kyoung Hwan Cho),김종철(Jong Cheol Kim),김재중(Jae Joong Kim),허호진(Ho Jin Heo ) 한국차학회 2018 한국차학회지 Vol.24 No.1

The antioxidant activities and hippocampal neuroprotective effects of tea (Camellia sinensis) seed oil against H 2 O 2 -induced oxidative stress were investigated to confirm its physiological effects. The antioxidant effects of tea seed oil were evaluated by measuring its 1,1-diphenyl-2-picrylhydrazyl, 2,2 -azino-bis (3-ethyl benzothiazoline-6-sulfonic acid) radical scavenging activities and malondialdehyde inhibitory effect. Tea seed oil was found to have significant antioxidant activity and inhibitory effect of lipid peroxidation. To confirm the ameliorating effect of the cholinergic system, the inhibitory effect of acetylcholinesterase (AChE) derived from PC12 was investigated. Tea seed oil was found to inhibit the AChE activity. Also, the protective effects of hippocampal neuronal cells against H 2 O 2 -induced neuronal cytotoxicity were investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide and 2 ,7 -dichlor-ofluorescein diacetate in HT22 cells. Tea seed oil was found to significantly increase the cell viability and reactive oxygen species scavenging activity.

뜰보리수 에탄올 추출물의 산화적 스트레스 억제 효과와 암세포 증식 억제 효과

오세인 ( Se In Oh ),이미숙 ( Mee Sook Lee ) 한국식품영양학회 2008 韓國食品營養學會誌 Vol.21 No.4

Elaeagnus multiflora, generally referred to as the cherry silverberry, is a plant. Elaeagnus multiflora fruit, leaves, and roots have been traditionally utilized in China as a treatment for cough, diarrhea, itch, and foul sores, and even cancer. More importantly, it is being investigated as a food that is capable of reducing the incidence of cancer, and also as a means of halting or reversing the growth of cancers. Considering the dearth of information regarding the medicinal properties of Elaeagnus multiflora, we assessed the antioxidative and cytotoxic effects of Elaeagnus multiflora by examining its scavenging effects on the 1,1-diphenyl-2-picryl-hydrazyl(DPPH) radical, its inhibitory effects on lipid peroxidation, and its inhibitory effects on cancer cell proliferation in HeLa cells, MCF-7 cells, and SNU-638 cells via MTT assay. Ethanol extracts of Elaeagnus multiflora flesh and seed inhibited DPPH radical production(36.91±1.00, 94.17±0.37) and lipid peroxidation (28.18±5.02, 40.30±1.45, respectively). The Elaeagnus multiflora seed is believed to exert a possible antioxidative effects against the DPPH radical. The ethanol extracts of Elaeagnus multiflora flesh and seed exerted the cytotoxic effects on Hela cells (6.93±1.92, 84.97±0.47), MCF-7 cells(5.45±0.41, 84.97±0.47), and SNU-638 cells(19.39±0.43, 76.84±0.63) used in this study. This result suggests that Elaeagnus multiflora seeds in contrast to its flesh, is believed to exert a possible anticancer effect. Elaeagnus multiflora seeds are considered to be a the candidate for preventative and dietetic treatment as an anticancer functional food.

Cisplatin의 세포독성에 대한 레몬밤 추출물의 억제 효과

최유선 ( Yu Sun Choi ),김선주 ( Sun Ju Kim ),유영월 ( Yeong Wol Yu ),임요섭 ( Yo Sup Rim ),황은희 ( Eun Hee Hwang ),이화정 ( Hwa Jeong Lee ),최은영 ( Eun Young Choi ),장병수 ( Byung Soo Chang ),정인주 ( In Ju Jung ) 대한미용학회(구 대한미용과학회) 2013 대한미용학회지 Vol.9 No.1

The purpose of this study is to evaluate the protective effect of Lemon balm (Mellisa officinalis) extract on the cytotoxicity induced by cisplatin, pharmaceutic alopecia inducer. To achieve this, cisplatin-induced cytotoxicity was assessed by XTT assay after human skin fibroblasts (Detroit 551) were cultured in media containing various concentrations of cisplatin. And also, the effect of vitamin E was examined on the cisplatin-induced cytotoxicity. For the protective effect of Lemon balm extract on cisplatin-induced cytotoxicity, Detroit 551 cells were pretreated with 80 or 100 μg/mL of Lemon balm extract for 2 hours, and also, antioxidative effects of Lemon balm extract such as 1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical scavenging activity and lactate dehydrogenase (LDH) activity were analysed. The results were as follows. Cisplatin showed a significant decrease of cell viability in dose dependently, and the XTT50 value was calculated at 25.7 μM. In the effect of vitamin E, it significantly increased cell viability which were decreased by the cisplatin-induced cytotoxicity. In the protective effect of Lemon balm extract on cisplatin-induced cytotoxicity, it significantly increased cell viability which was decreased by cisplatin-induced cytotoxicity, and also it showed the DPPH-radical scavenging activity and LDH inhibitory activity. Therefore, these results suggested that the cytotoxicity of cisplatin may be involved in oxidative stress, and also, Lemon balm extract effectively prevented the cytotoxicity induced by cisplatin via antioxidative effect. Conclusively, the natural extract like Lemon balm may be useful for the development as antioxidative agent via the prevention of the cytotoxicity induced by pharmaceutic alopesia inducer correlated with oxidative stress.

독성물질인 염화카드뮴으로 손상된 배양 NIH3T3 섬유모세포에 대한 애기똥풀 추출물의 항산화 효과

김태윤 ( Tae Yoon Kim ),제갈승주 ( Seung Joo Jekal ) 대한임상검사과학회 2016 대한임상검사과학회지(KJCLS) Vol.48 No.1

The aim of this study was to evaluate the cytotoxicity of cadmium chloride (CdCl2), toxicant, and the protective effect of Chelidonium majus (CM) extract on CdCl2-induced cytotoxicity in cultured NIH3T3 fibroblasts. Cell viability, the effect of butylated hydroxytoluene (BHT) against CdCl2, and the antioxidative effects including DPPH-free radical scavenging activity, superoxide anion-radical scavenging activity (SSA), and lactate dehydrogenase (LDH) activity were assessed. CdCl2 caused a significant dose-dependent decrease in cell viability, and XTT50 value was determined at 38.7 uM of CdCl2. It was determined as highly-toxic by Borenfreund and Puerner`` toxic criteria. BHT of antioxidant significantly increased cell viability severely damaged by CdCl2-induced cytotoxicity in these cultures. In the protective effect of CM extract on CdCl2-induced cytotoxicity, CM extract significantly increased cell viability, DPPH-free radical scavenging activity, SSA and inhibitory activity of LDH. From these results, it is suggested that oxidative stress is involved in the cytotoxicity of CdCl2, and CM extract showed protective efficacy on CdCl2-induced cytotoxicity via antioxidative effects. Conclusively, natural resources like CM extract may be a putative antioxidative agent for the detoxification or diminution of toxicity correlated with oxidative stress.