Enalapril 에 의한 기침 발생율

노승익(Seung Ik Roh),김명선(Myeong Seon Kim),김은실(Eun Sil Kim),서두선(Du Seon Seo),정내인(Nae In Jeong),이명숙(Myeong Suk Lee),한승수(Seung Soo Han),김광희(Kwang Hoi Kim),김윤권(Yoon Kweon Kim) 대한내과학회 1994 대한내과학회지 Vol.47 No.1

N/A Background: Enalapril is an angiotensin-converting enzyme inhibitor that has been widely used in treating hypertension, congestive heart failure, DM and various renal diseases. Cough has been recognized as a side effect of angiotensin-converting enzyme inhibitors. Therefore we studied the incidence and the chracteritics of Enalapril induced cough in Korea. Method: The sixty eight patients with Enalapril who visited out-patients department of internal medicine, Han-Il General Hospital during the period from april 1992 to october 1992 were included in this study. In all patients careful history taking and review of chart was done. Patients with respiratory disease were excluded. The criteria of angiotensin converting enzyme inhibitor induced cough was that cough developed after administration of Enalapril and improved after discontinuing drug and readministration of drug induce resume of symptom. Resnlts: Subjects include 34 males, 34 females and their average age was 53.3 years, Diseases of theirs were hypertension (57 cases}, congestive heart failure (5 eases), various renal disease (28 cases) and 9 patients of them were smokers. Average drug dosage was 11.7 mg a day and average administration duration was 182 days. Of the 68 patients, 23 patients (33.8%) had chronic cough due to enalapril, which had 12 male and 11 female patients. The cough disappearing time after withdrawal of the angiotensin-converting enzyme inhibitors was average 7.8 days. In 16 patients (two thirds of them), cough disappeared within 4 days after drug withdrawal. 9 patients had to stop taking drug because of severe cough. Generally the characters of cough induced by angiotensin-converting enzyme inhibitors were dry, persistent, tickling and tended to develope especially at night, Conclusion: Cough is not uncommon side effect in angiotensin-converting enzyme inhibitor users. There fore clinician should consider angiotensin-converting enzyme inhibitor induced cough initially when cough developed after angiotensin-converting enzyme inhibitor administration.

Angiotensin 전환효소 유전자 다형성과 양극성 장애

김경나,김종우,정주호,이기철,정홍경,임성빈 대한생물치료정신의학회 2003 생물치료정신의학 Vol.9 No.2

연구목적 : 감정조절에 Renin-Angiotensin System이 관여하는 것으로 보고되어 왔다. 그 근거로 우울증의 새로운 약물치료로 주목받고 있는 Substance P의 대사에 angiotensin 전환효소(angiotensin-converting enzyme, ACE)가 관여한다는 것과 고혈압 환자에서 angiotensin 전환효소 억제제(ACE inhibitor)를 사용했을 때 다행감이나 우울감을 초래한다는 것 등이 있다. 본 연구에서는 ACE의 유전자 다형성을 분석하여 양극성장애와 ACE와의 연관성을 알아보고자 하였다. 방 법 : 양극성장애로 진단된 환자군 82명과 대조군 135명을 대상으로 16번째 intron의 다형성 부위를 가진 시발체 쌍(primer pair)을 사용하며 중합효소 연쇄반응(Polymerase Chain Reaction, PCR)을 시행하여 490bp 산물(I allele)과 190bp 산물(D allele)을 관찰함으로써 ACE 유전자의 유전자형(genotype)의 발현율과 대립유전자(allele)의 빈도를 비교 분석하였다. 결 과 : 양극성장애 환자군과 대조군 사이에서 유전자형의 발현율과 대립유전자적 빈도는 유의한 차이가 없었다. 결 론 : 양극성장애 환자군과 ACE 유전자 다형성 사이에 유의한 관련성은 없었다. 이 결과는 ACE가 양극성 장애의 원인으로 주요한 역할을 한다는 것을 시사하지 못했다. Objectives : A possible participation of the Renin-Angiotensin Systern(RAS) in regulating of the mood has been suggested by reports as follows : the angiotensrn converting enzyme(AGE1 is involved jn the metabolism of the neuropeptide substance P impficated with novel strategies for the pharmacotherapy of depression and the use of ACE inhibitors in hypertensive patients has been associated with euphoric or depressive stales. The aim of the present study was to analyze the role of the ACE gene I/D polymorphism for bipolar disorder. Methods : We examined the frequency of a polymorphism characterized by the insertion or deletion of a 287-bp Alu repeat sequence in intron 16 of the angiotensin converting enzyme gene(located on chromosome 37q233 in groups of patients with bipolar disorder(n=82) compared to healthy control subjects[n=135). ACE genotype was determined by size-analysis of polymerase chain reaction products. Results : The ACE ID polymorphism did not show any difference in allelic frequencies and genotypic distributions between bipolar disorder patients and control subjects. Conclusions : No significant association was found with bipolar disorder and the polymorphism of ACE gene. This finding does not support that ACE I/D polymorphism is a significant risk factor for bipolar disorder.

비당뇨병성 만성 신질환 환자에서 안지오텐신 수용체 길항제의 단백뇨 감소 효과에 대한 안지오텐신 전환 효소 유전자 다형성의 영향

최훈영 ( Choe Hun Yeong ),허종호 ( Heo Jong Ho ),김현욱 ( Kim Hyeon Ug ),김현진 ( Kim Hyeon Jin ),김형종 ( Kim Hyeong Jong ),김범석 ( Kim Beom Seog ),박형천 ( Park Hyeong Cheon ),강신욱 ( Kang Sin Ug ),최규헌 ( Choe Gyu Heon ) 대한신장학회 2004 Kidney Research and Clinical Practice Vol.23 No.1

목 적 : 단백뇨를 동반한 당뇨병성 및 비당뇨병성 만성 신질환 환자에서 안지오텐신 전환효소 억제제의 단백뇨 감소 효과가 안지오텐신 전환효소 [angiotensin-converting enzyme (ACE)] 유전자 다형성에 의해 영향을 받는다는 보고가 있었다. 그러나 이들 환자에서 안지오텐신 수용체 길항제의 단백뇨 감소 효과가 ACE 유전자 다형성에 의해 영향을 받는지는 확실히 규명되어 있지 않은 실정이다. 본 연구에서는 비당뇨병성 만성 신질환 환자에서 안지오텐신 수용체 길항제의 단백뇨 감소 효과와 ACE 유전자 다형성을 검사하여 안지오텐신 수용체 길항제의 단백뇨 감소효과가 각각의 ACE 유전자 다형성에 따라 차이가 있는지를 분석하고자 하였다. 방 법 : 연세대학교 세브란스병원 신장내과에 내원하거나 입원한 환자 중 일일 500 ㎎ 이상의 단백뇨를 동반한 비당뇨병성 만성 신질환 환자 중 본 연구에 동의한 총 70명의 환자를 대상으로 전향적 연구를 시행하였다. 안지오텐신 수용체 길항제인 losartan을 연구시작 시점부터 12주까지 50 ㎎, 12주부터 24주까지 100 ㎎으로 증량하여 투여하면서 4주 간격으로 추적 관찰 하였다. 결 과 : 대상환자 70명에서 losartan 투여 전에 비해 12주 후 및 24주 후 수축기 혈압, 이완기 혈압, 평균 동맥압 등은 의의있게 감소하였다. 혈청 콜레스테롤과 중성지방은 12주 후 및 24주 후에 유의하게 감소하였으며, 혈청 알부민은 기저치에 비해 12주 후에 의의있는 증가 소견을 나타내었고, 혈청 크레아티닌의 유의한 차이는 없었다. 24시간 요단백 배설량은 losartan 투여 후 12주와 24주에서 의의있게 감소하였으며, 크레아티닌 청소율은 12주 후에는 변화가 없었으나, 24주 후 유의하게 감소하였고, 24시간 요나트륨 배설량은 의의있는 변화를 보이지 않았다. Losartan 투여 후 12주 및 24주에서의 24시간 요단백 배설량 감소 정도는 투여전의 요단백 배설량과 유의한 양의 상관관계를 보였다. Losartan 투여 12주 후 30% 이상의 단백뇨 감소 효과를 보인 환자군을 반응군 (N=50)으로 설정하여 비반응군 (N=20)과 비교 분석한 결과 losartan 투여전 혈압, 혈청 요소질소, 혈청 크레아티닌, 그리고 24시간 요단백 배설량에는 유의한 차이가 없었으나, 12주 및 24주 후 수축기 혈압, 평균 동맥압은 반응군에서 의의있게 낮았다. 24시간 요단백 배설량은 12주 후 반응군에서 유의하게 낮았으며 24주 후에도 반응군에서 낮은 경향을 보였으나 양 군간에 통계학적으로 의의있는 차이는 없었다. 한편 ACE 유전자형에 따른 기저 혈압 및 24시간 요단백 배설량에는 유의한 차이가 없었으며, losartan 투여 12주 및 24주 후 혈압의 변화, 24시간 요단백 배설량의 변화, 크레아티닌 청소율의 변화, 그리고 반응을 보인 환자의 빈도에는 의의있는 차이가 없었다. 결 론 : 이상의 연구결과로 비당뇨병성 만성 신질환 환자에서 안지오텐신 수용체 길항제는 혈압 및 요단백 배설을 유의하게 감소시킴을 관찰하였으며, 이러한 안지오텐신 수용체 길항제의 혈압 및 단백뇨 감소 효과는 안지오텐신 전환효소 유전자 다형성에 의해 영향을 받지 않음을 관찰하였다. Background : Angiotensin Ⅱ, a potent vasoconstrictor, plays a key role in renal injury and in the progression of chronic renal disease of diverse causes. In every organ system, the biologic effects of angiotensin Ⅱ are mediated through its interaction with specific receptors on cell membranes. Angiotensin Ⅱ receptor antagonist specifically inhibits angiotensin Ⅱ-mediated physiologic responses such as systemic and renal vasoconstriction, sodium reabsorption by renal proximal tubule, and stimulation of aldosterone and adrenergic hormone release by the adrenal gland. It has been reported that losartan angiotensin Ⅱ receptor antagonist, has a significant antiproteinuric effect in patients with diabetic and non-diabetic renal disease. This study was carried out to investigate the effect of angiotensin-converting enzyme (ACE) gene polymorphism on the renal response to angiotensin Ⅱ receptor antagonist in non-diabetic proteinuric chronic renal patients. Methods : Seventy patients with non-diabetic chronic renal disease with urinary protein excretion greater than 500 ㎎/day were enrolled in this prospective study. Subjects were given losartan 50 mg o.d. for the first 12 weeks, and then were given to 100 mg o.d. for another 12 weeks. Results : Twelve weeks and twenty-four weeks later, blood pressure, urinary protein excretion, total cholesterol, and triglyceride decreased significantly compared with baseline values. There was a significant correlation between the levels of baseline urinary protein excretion and the magnitudes of the reduction of urinary protein excretion after treatment with losartan. Baseline blood pressure, BUN, serum creatinine, and urinary protein excretion were not different in the responder group (patients with more than 30% reduction of urinary protein excretion after losartan treatment) compared with the nonresponder group. Systolic blood pressure and mean arterial pressure in the responder group were significantly lower than the nonresponder group after twelve and twenty-four weeks. Urinary protein excretion in the responder group was significantly lower than the nonresponder group after twelve weeks. When the patients were divided into three groups according to ACE gene polymorphism, Ⅱ, ID and DD, there were no significant differences in the blood pressure change, reduction of urinary protein excretion following losartan treatment and distributions of responder among three groups. Conclusion : Our results suggest that angiotensin Ⅱ receptor antagonist, losartan, significantly reduced blood pressure and proteinuria in patients with non-diabetic chronic renal disease. The magnitude of antiproteinuric effect of losartan was not influenced by ACE gene polymorphism. However, further studies with large number of patients are required to confirm the issues regarding the ACE gene polymorphism and the antiproteinuric effects of angiotensin disease. (Korean J Nephrol 2004;23(1):46-56)

Angiotensin-Converting Enzyme Inhibitor(ACE Inhibitor)에 의해 유발된 안면부 맥관부종(angioedema) 치험례

하유군,정기용,백종우,김동우,박종형,전찬용,최유경,Hsia, Yu-Chun,Jung, Ki-Yong,Baik, Jong-Woo,Kim, Dong-Woo,Park, Jong-Hyung,Jun, Chan-Yong,Choi, You-Kyung 대한한방내과학회 2007 大韓韓方內科學會誌 Vol.28 No.2

Angioedema is a localized transient swelling of sudden onset that can occur in the face, lips, tongue, hand, feet, respiratory system and gastrointestinal system. Angioedema is classified as allergy, hereditary, idiopathic or induced by medication as like aspirin, nonsteroidal anti-inflammatory agents, opiates, antibiotics, and angiotensin-converting enzyme. Angiotensin-converting enzyme inhibitors are widely prescribed for hypertension and heart failure. This drug is commonly associated with angioedema which may be potentially life threatening. We experienced a case of angioedema induced by ACE inhibitor (angiotensin-converting enzyme inhibitor) in a 74-year-old patient who took ACE inhibitor to control hypertension during hospitalization. We thought the angioedema in the face had relation to myenzhong (面腫, mienjong) in oriental medicine. Weiling-tang (Wiryung-tang) was effective for angioedema in the face. As a result the symptoms disappeared rapidly. After 6 days, the patient's symptoms had notably improved.

장기적인 Captopril 투여가 백서 소장 점막의 Angiotensin Converting Enzyme 활성도에 미치는 영향

김나영(Na Young Kim),김웅(Woong Kim),안준석(Zoon Seog Ahn),양석균(Suk Kyun Yang),이풍렬(Poong Lyul Rhee),정현채(Hyun Chae Jung),이효석(Hyo Suk Lee),윤용범(Yong Bum Yoon),송인성(In Sung Song),김정룡(Chung Yong Kim) 대한내과학회 1989 대한내과학회지 Vol.37 No.1

N/A Angiotensin-converting enzyme (ACE, c-terminal dipeptidyl carboxypeptidase) plays a key physiological role in the regulation of blood pressure through conversion of angiotensin I to angiotensin II and inactivation of bradykinin. Although this enzyme has been mainly found in vascular endothelial cells, recently it has been identified as a prominent mucosal brush border membrane bound enzyme in the small intestine and is estimated to take part in the digestion and absorption of dietary protein. The authors designed the following experiment to investigate the effect of long-term captopril administration, which is used broadly as an antihypertensive agent and is an active site specific ACE inhibitor, to mucosal ACE specific activity in rat small intestine. Twenty-three 215 g average weight rats were divided into three groups; the control group, consisting of 9 rats, was not fed with captopril; the second group, consisting of 9 rats, was fed with 2.5 mg/kg captopril dissolved in distilled water; and the third group, consisting of 5 rats, was fed with 12.5 mg/kg . The rats were killed by decapitation and the isolated small intestines were divided into three segments of equal length from the Treitz ligament to the terminal ileum, After the mucosal homogenate was obtained from each segment, ACE specific activities and the stable marker enzymes known for the brush border membrane (aminopeptidase N, dipeptidyl aminopeptidase IV, alkaline phosphatase) were assayed. The results were as follows; 1) ACE specific activities were highest in the middle segment and lowest in th distal segment, and there was no difference in ACE specific activities between the control group and 2.5 mg/kg or 12.5 mg/kg captopril treated groups (p>0.05). 2) There was no difference in aminopeptidase N, dipeptidyl aminopeptidase IV, and alkaline phosphatase specific activities between the control group and captopril treated groups (p>0.05). We concluded that mucosal ACE specific activities in rat small intestine were not inhibited by long-term captopril administration, and that the estimated role of rat intestinal ACE in the digestion and absorption of dietary protein would not be altered by long-term captopril administration.

안지오텐신 전환효소 유전자 다형성이 심폐지구력 요인에 미치는 영향

이경진(Lee Gyeong Jin),김지연(Kim Ji Yeon),김재등(Kim Jae Deung) 한국사회체육학회 2003 한국사회체육학회지 Vol.20 No.2

The purpose of this study was designed to investigate the effects of Angiotensin-Converting enzyme gene polymorphism on factors of cardiovascular fitness. The subjects of the survey for this study were performed by 57 men who were mostly healthy students in the twentieth ages, found and searched in the University and who had any history of cardiovascular disease. They were classified into three groups by their Angiotensin-Converting enzyme gene polymorphism: II genotype group(n= 25) , ID genotype group(n=9) which was earned by blood test. The experiments were tested with treadmill running on a motor-driven treadmill. Subjects of this study were exercised according to modified bruce protocol which is commenced with 1.7 mph ar 10% grade for 3 minute, then progress with 0.8~0.9 mph and 2% grade increase every three minute. The experiment data was analysed for the levels of VO2max, maximal heart rate response between the groups. This experiment had indicated the effects of Angiotensin-Converting Enzyme Gene Polymorphism on Factors of Cardiovascular Fitness; VO2max, Maximal heart rate and Rest heart rate. The Statistical techniques for data analysis were one-way ANOVA to determine the difference among groups. The 5% level of significance was used as the critical level for acceptance of hypotheses for the study. The results of this study are following: 1. There was no significant difference in VO2max among the groups. 2. There was no significant difference in MHR(Maximal Heart Rate) among the groups. 3. There was no significant difference in RHR(Rest Heart Rate) among the groups. In conclusion, VO2max, Maximal Heart Rate and Rest Heart Rate there had no significant differences in each Angiotensin-Converting enzyme gene polymorphism groups. Therefore, Angiotensin-Converting enzyme gene polymorphism had not influenced any significant in VO2max, Maximal heart rate and Rest heart rate in healthy male.

혈액순환과 관련하여 nitric oxide 생성과 angiotensin converting enzyme 활성에 미치는 Acai berry 에탄올 추출물의 영향

남향(Hyang Nam),이수경(Su-Gyeong Lee),김덕원(Deok Won Kim),김성구(Sung Goo Kim),김기영(Ki Young Kim),김주완(Joo Wan Kim),김문무(Moon-Moo Kim),정경태(Kyung Tae Chung) 한국생명과학회 2013 생명과학회지 Vol.23 No.6

본 연구의 목적은 혈관계 순환을 촉진시키는 약효제를 발굴하는 것이다. 아사이베리(Acai berry) 에탄올 추출물(ABEE)의 항산화 효과 뿐만 아니라 토끼의 폐에서 유래한 angiotensin converting enzyme (ACE)의 활성 및 대식세포와 혈관내피세포에서 nitric oxide 생성에 대한 효과가 본 연구에서 조사되었다. 먼저 환원력과 지질과산화에 대한 ABEE의 항산화효과가 관찰되었다. 뿐만 아니라, ABEE는 hydroxyl radical에 의하여 유발된 DNA 산화에 대한 보호효과를 보여주었다. 더욱이 ABEE는 0.01%의 농도에서 약 50% 정도의 angiotensin converting enzyme 활성에 대한 억제효과를 발휘하는 것으로 나타났다. 혈관내피세포에서 ABEE는 nitric oxide 의 생성을 증가시켰으나 대식세포에서는 lipopolysaccharide에 의하여 생성된 nitric oxide 생성을 감소시켰다. 게다가 superoxide dismutase (SOD)-2와 -3 발현수준은 ABEE 처리에 의하여 증가되었으나 SOD-1의 발현수준은 일정하였다. 더욱이 nitric oxide synthases-1 (NOS-1)의 발현수준은 ABEE 처리에 의하여 증가되었으나, 유발 효소인 NOS-2의 발현수준은 일정하였다. 또한 SOD의 전사인자인 Nrf-2의 발현수준은 ABEE에 의하여 증가되었다. 그러므로 이러한 결과들은 ABEE가 이상의 작용을 경유하여 혈액순환을 촉진시킬 수 있고, 혈관의 건강을 위한 큰 잠재성을 가지고 있다는 것을 암시하고 있다. The aim of this study is to develop a supplementary therapeutic agent capable of promoting vascular circulation. The effects of Acai berry ethanolic extracts (ABEE) on activity of angiotensin converting enzyme from rabbit lung, production of nitro oxide in both murine macrophage cells and vascular endothelial cells as well as antioxidant effects were investigated in this study. First of all, it was observed the direct effects of ABEE on reducing power and antioxidant effect lipid peroxidation. In addition, ABEE showed a protective effect on DNA oxidation induced by hydroxyl radical. Furthermore, ABEE at 0.01% exerted approximately 50% inhibition on activity of angiotensin converting enzyme. ABEE increased the production of nitric oxide in endothelial cells, but decreased the induction of nitric oxide stimulated by lipopolysaccharide in microphage. The expression levels of superoxide dismutase (SOD)-2 and -3 were enhanced by ABEE treatment, however, the expression level of SOD-1 remained constant. Moreover, the expression level of nitric oxide synthases-1 (NOS-1), a constitutive enzyme, was increased by ABEE, but that of NOS-2, a inducible enzyme, was constant. It was also found that the level of Nrf-2, a transcription factor of SOD, was increased by ABEE. Therefore, these results demonstrate that ABEE could promote blood circulation via above actions, suggesting that may be helpful for health of blood vessel.

토끼의 난소 과자극증후군 유발에 미치는 내인성 Angiotensin 2의 작용 및 Angiotensin-converting Enzyme Inhibitor의 치료효과

박원일 ( Won Il Park ),송찬호 ( Chan Ho Song ),김병길 ( Pyung Kil Kim ),조동제 ( Dong Je Cho ),박기현 ( Ki Hyun Park ),장병철 ( Byung Chul Chang ) 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.12

Ovarian hyperstimulation syndrome is most serious complication during ovulation induction. Although the incidence of this disease is increasing, the pathophysiology remains uncertain. Consensus is that main pathophysiology of the disease is increased vascular permeability, but the etiologic agent causing hyperpermeability is still unknown, Prostaglandin, histamine, angiotensin, some cytokines and growth factors have been suspected as etiologic agent. At present, angiotensin is mostly suspected agent of this disease. The purpose of this study is revealing etiologic role of angiotensin II in ovarian hyperstimulation and assessing the therapeutic or preventive effect of lowering angiotensin II by angiotensin converting enzyme inhibitor. After developing of ovarian hyperstimulation in rabbit, Captopril were administerd in study group. The correlations between angiotensin II and clinical parameters of severity of the disease such as ovarian volume, amount of ascites or changes of hematocrit were assessed. The correlations between angiotensin II and prostaglandin or estradiol were also assessed. The Microfil was perfused through the ovarian arteries and morphology of vascularities were revealed. The differences in ovarian volume, amount of ascites, changes of hematocrit, prostaglandin and estradiol were checked between study and control group. The parameters of severity of ovarian hyperstimulation were closely related with the level of angiotensin II. The levels of prostaglandin and estradiol were also positively correlated with the level of angiotensin II. The degree of neovascularization tended to be increased in control group, but the individual variations were existed. The parameters of severity of ovarian hyperstimulation were markedly improved in captopril group. From this data, we can conclude that angiotensin II is related with development of ovarian hyperstimulation syndrome and angiotensin converting enzyme inhibitor can prevent the development of this disease.

Optimizing Preparation Conditions for Angiotensin-I-Converting Enzyme Inhibitory Peptides Derived from Enzymatic Hydrolysates of Ovalbumin

Qun Huang,Shu-gang Li,Hui Teng,Yong-guo Jin,Mei-hu Ma,Hong-bo Song 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.6

Angiotensin-I-converting enzyme (ACE) inhibitory peptides were prepared from ovalbumin using enzyme hydrolysis with pepsin as an enzyme source. Effects of pH, enzyme dosage, substrate concentration, hydrolysis temperature, and time on the degree of hydrolysis and the ACE inhibition rate were investigated using single factor experiments. Preparation conditions for ACE inhibitory peptides were optimized using a response surface design on the base of single factor experiments. Optimum preparation conditions were a substrate concentration of 5.2 g/100 mL of D.W with a pH value of 2.5, an enzyme dosage of 14,000 U/g, and a hydrolysis time of 250 min at 30℃. The ACE inhibition rate was up to 70.55±1.13% under these conditions.

고 Proline식에 의한 백서소장의 Angiotensin Converting Enzyme 특이활성도의 유도

윤병철 ( Yun Byeong Cheol ),김재준 ( Kim Jae Jun ),송인성 ( Song In Seong ),김정룡 ( Kim Jeong Lyong ) 대한내과학회 1993 대한내과학회지 Vol.44 No.6

연구배경 : ACE(Angiotensin Converting Enzyme)는 carboxy terminal로부터 dipeptide를 분해하는 carboxy peptidase로 여러 기관의 혈관내피세포, 신세뇨관과 소장의 상피세포 등에 분포하고 있는 것으로 알려져 있다. 이 ACE가 소장 미소융모막에 존재함이 알려지고 소장에서는 기존의 작용외에 단백질의 분해에도 관여함이 시사되어, 소화효소로서의 중요성이 부각되고 있다. 음식물에 포함되어 있는 기질의 양에 따라 이 기질의 분해 및 흡수에 관여하는 효소들의 활성도가 변화한다는 것은 이미 널리 알려져 있다. ACE는 특이적으로 대부분의 다른 단백분해효소들이 분해하지 못하는 prolyl bond를 분해시키는 것으로 알려져 있으므로, 이에 저자들은 고 proline식으로 젤라틴을 투여하였을 때 여러 가지 단백분해효소들의 변화, 특히 ACE의 변화를 관찰하여 실제로 생체내에서 ACE가 소화효소로서의 역할을 담당하고 있는가를 확인하기 위해 이 연구를 시행하였다. 방법 : 150-200gm 가량되는 Wistar rat 14마리를 2군으로 나누어, 대조군은 일반 실험실용 쥐사료를 자유롭게 먹게하고, 실험군은 하루에 1마리당 20% 젤라틴 50gm과 대조군이 소비하는 일반사료의 절반씩을 3주간 먹게하였다. 소장을 적출하여 같은 크기로3등분한 후 각각에서 점막균질액과 미소융모막을 만들고 각각에서 단백질과 ACE, DAP-Ⅳ, aminopeptidase N, sucrase, alkaline phosphatase의 특이활성도를 측정하여 양군을 비교하였다. 결과 : ACE의 특이활성도는 점막균질액에서는 젤라틴군이 근위부와 중간부에서 각각 19.6±3.6, 1.9±2.3으로 대조군의 12.0±1.4, 8.5±1.9에 비해 유의하게 높았으며(p<0.005 & 0.05), 미소융모막에서는 젤라틴군이 근위부에서 174.2±70.9로 대조군의 103.1±26.0에 비해 유의하게 높았고(p<0.05), 다른 부위에서는 유의한 차이가 없었다(nM/minmg protein). DAP-Ⅳ의 특이활성도는 점막균질액에서는 젤라틴군이 근위부와 중간부에서 56.2±7.9, 83.3±9.3으로 대조군의 43.3±4.5, 65.9±7.6에 비해 유의하게 높았으며(p<0.05), 미소융모막에서는 젤라틴군이 근위부에서만 397.1±32.1로 대조군의 315.3±60.2에 비해 유의하게 높았고(p<0.05), 다른 부위에서는 유의한 차이가 없었다(nM/minmg protein). Aminopeptidase N, sucrase, alkaline phosphatase들의 특이활성도는 점막균질액에서는 모두 원위부에서만 대조군이 젤라틴군에 비해 유의하게 높았고(p<0.05), 다른 부위에서는 유의한 차이가 없었다. 결론 : ACE가 특이적으로 분해할 수 있는 prolyl bond가 많은 고 proline식으로 젤라틴을 투여하였을때, 기존에 생체내에서 prolyl bond의 소화에 관여하는 것으로 알려진 DAP-Ⅳ와 함께 ACE의 특이활성도가 증가함이 확인되어, 이 ACE가 DAP-Ⅳ와 마찬가지로 생체내에서 prolyl bond가 포함된 단백질의 분해에 중요한 역할을 담당하고 잇Dma을 강력히 시사하고 있다고 생각된다. Objectives : Aminopeptidase N and DAP-Ⅳ (dipeptidyl aminopeptidase Ⅳ) have been established as major functional peptidases in BBM (brush border membrane). Recent reports indicate that ACE (angiotensin converting enzyme), carboxypeptidase P and several endopeptidases are found in BBM. Proline rich proteins such as casein and collagen are important dietary constituents. Prolyl poptide bonds are generally resistant to the action of several peptidases. DAP-Ⅳ and ACE are known as prolyl bond specific peptidases in BBM. The role of intestinal ACE as a digestive enzyme in vivo is not known. The dietary regulation of intestinal enzymes has been well established. The present study was designed to investigate the response of intestinal ACE to the high proline diet (gelatin), and therefore evaluate the role as a digestive enzyme. Methods : Total 14 male Wistar rats were divided in 2 groups. Control group was maintained on standard chow diet, and gelatin group was maintained on 20% gelatin gel 50gm/rat/day and half of standard chow diet for 3 weeks. Mucosal homogenate and BBM were prepared. The protein level and specific activities of ACE, aminopeptidase N, DAP-Ⅳ, sucrase and alkaline phosphatase were measured n homogenate and BBM. Results: 1) The specific activities of ACE and DAP-Ⅳ were significantly induced in mucosal homogenates of proximal and middle intestines of gelatin group compared with those of control (p<0.05) (Table 3, 4). 2) The specific activities of aminopeptidase N, sucrase and alkaline phosphatase were not induced in mucosal homogenate and BBM of gelatin group compared with those of control. Conclusion : The specific activities of ACE and DAP-Ⅳ were significantly induced by high proline diet. This result suggests that intestinal ACE may play a physiologic role in protein digestion like DAP-Ⅳ.