위암조직에서 Ribonuclease와 Ribonuclease Inhibitor의 상호작용에 관한 연구

박경남,안광무,고재경 한양대학교 의과대학 1994 한양의대 학술지 Vol.14 No.1

In order to understand the processes involved in carcinogenesis and suppression of stomach cancer, activities of ribonuclease(RNase) and RNase inhibitor were measured in the stomach cancer tissue and were compared with those in the control tissue of the stomach. Also separated were RNases and RNase inhibitors from stomach cancer tissue by a DEAE cellulose colimn chromatography to find out the RNase and RNase inhibitor specific to the stomach cancer. Activities of acid and neutral RNase were signigicantly decreased in stomach cancer tissue and positive rates of the enzymes as markers for the stomach cancer were high suggesting the use of RNases as a biochemical marker for the stomach cancer. Activity of RNase inhibitor expressed as latent RNase activity was unchanged, although it tended to be decreased. RNases in the stomach cancer tissue was separated by a DEAE-cellulose column chromatography into six isozymes, of which RNase isozyme Ⅱ was found to be specific to the cancer and RNase isozyme V was activated. The RNase isozyme Ⅲb isolated in the control tissue disappeared from the cancer tissue. Of the RNase isozymes isolated from the stomach cancer tissue, RNase Ⅰ,Ⅱ and Ⅲ isozymes were nonseretory type of RNase active against RNA as substrate, RNase Ⅳ and Ⅴ isozymes secretory type of RNase active against poly C and RNase Ⅳ mixed type of RNase. On the other hand, RNase isozymes from the control tissue of the stomach were all secretory type of RNase except for RNase isozyme I. This indicated that RNase isozymes Ⅲ and Ⅵ isolated from the cancer tissue appeared to be different in nature from those from the control tissue. Activity of RNase inhigitor measured with poly C as a substrate was increased in RNase isozymes Ⅳ,Ⅴ and Ⅵ of the stomach cancer tissue, being increased greatly in the RNase isozyme Ⅴ. Observations that the RNase isozyme V isolated from the stomach cancer tissue was activated and exhibited higher acctivity of RNase inhibitor suggested that interaction between RNase isozyme V and RNase ingibitor play an important role in carcinogenesis and suppression of stomach cancer.

위암의 생화학적지표에 관한 연구

강성관,고재경,박경남 한양대학교 의과대학 1993 한양의대 학술지 Vol.13 No.2

In order to find out biochemicla markers for adenocarcinoma of stomach, nucleic acid and protein contents, activities of deoxyribonuclease(DNase), ribonuclease(RNase), 5'-nucleotidase and amylase were determined in gastric juice and stomach cancer tissues of patients with gastric cancer. Also analysed were the enzymes and proteins isolated from gastric juice and stomach cancer tissue by a DEAE-cellulose column chromatography to investigate the specific tumor markers for stomach cancer. Concentrations of DNA and protein were significantly increased in the stomach cancer tissue, but RNA content was nuchanged. Activities of acid DNase and 5'-nucleotidase were significantly decreased in stomach cancer tissue, but increased in gastric juice of patients with stomach cancer. A significant activity of amylase, undetected in control stomach tissue and gastric juice, was observed in some of stomach cancer tissue and gastric juice of patients with stomach cancer. Neutral RNase and acid DNase were isolated from stomach cancer tissue by a DEAE-cellulose column chromatgraphy into single peak each, suggesting the absence of the RNase and DNase isozymes specific to the cancer in the stomach cancer tissue. Two peaks each for neutral RNase and amylase ioszymes were isolated by a DEAE-cellulose column chromatography from gastric juice of patient with stomach cancer. The present study indicated that DNA and protein contents and activities of acid DNase, neutral RNase, 5'-nucleotidase and amylase in stomach tissue, and activities of neutral RNase and amylase in gastric juice coluld be used for biochemical markers for stomach cancer. Also indicated was the use as specific tumor markers of RNase and amylase isozyme isolated from gastric juice of patients with stomach cancer. The appropriate combination of two or more tumor markers might improve the specificity for the detection of stomach cancer.

위암 환자의 위암조직과 위액에서 위암에 특이한 단백에 관한 연구

박경남,신창록,고재경 한양대학교 의과대학 1994 한양의대 학술지 Vol.14 No.1

In order to investigate cancer specific proteins associated with stomach cancer, proteins of stomach cancer tissue and gastric juice from patients with stomach cancer were separated by a DEAE-cellulose column chromatography, high performance liquid chromatography(HPLC) and polyacrylamide gel electrophoresis, and the results were compared with those from control tissue and gastric juice. Protein concentration of cancer tissue and gastric juice of patients with stomach cancer was significantly increased and positive rate of protein contents in stomach cancer tissue and gastric juice as a marker for stomach cancer was high, indicating the possible use of of the protein contents as a biochemical marker for the stomach cancer. Proteins in the stomach cancer tissue were separated by a DEAE cellulose column chromatography into 6 peak proteins, of which a single peak protein (peak Ⅲb) was found to be specific to the cancer and proteins in the gastric juice from patient with stomach cancer were separated into 8 peak proteins, of which three protein peaks(peak Ⅱ, Ⅲ and Ⅳb) were specific to the cancer. Peak Ⅰ and Ⅴ proteins isolated from both cancer tissue and gastric juice from patient with stomach cancer were observed to be activated, suggesting a possible role of these proteins in carcinogenesis and suppression of the stomach cancer. Isolation patterns for proteins of the stomach cancer tissue appeared to be different form those of the control tissue, showing presence of more than 8 protein bands specific to the cancer and disappearance of more than 2 protein bands specific to the cancer and disappearance of more than 2 protein bands from the stomach cancer tissue. The results indicated that changes in proteins of cancer tissue and gastric juice from patient with stomach cancer did not take place in a single protein, but did occur in multiple proteins. Qualitative changes in the proteins were variable in nature, such as presence of cancer specific proteins, disappearance of proteins from the cancer tissue and gastric juice and activiation of the proteins.

위암환자의 위액단백과 Amylase의 분리와 성상에 관한 연구

정부근,고재경,노연희 한양대학교 의과대학 1992 한양의대 학술지 Vol.12 No.1

Amylase activity was measure din gastric juice and cancer tissue of patients with stomach cancer to find out the possible use of the enzyme as a biochemical marker for stomach cancer. Amylase and proteins in gastric juice from patients with stomach cancer were separated by a DEAE-cellulose column chromatography to investigate the enzyme and proteins specific to the stomach cancer. (1)Protein content and amylase activity were not changed in gastric juice of patients with gastritis and duodenal ulcer, but significantly increased in gastric juice of patients with stomach cancer. The positive rate of protein content and the enzyme activity in the gastric juice as a marker for stomach cancer was relatively high. (2)Protein content in the stomach cancer tissue was significantly increased and amylase activity was greatly increased in 3 cases out of 12 cases of the stomach cancer tissues studied. (3)Substrate specificity and effect of halogen ions on amylase in gastric juice of patient with the stomach cancer was similar to those on the enzyme in the stomach cancer tissue. (4)DEAE-cellulose columkn chromatography revealed that proteins in gastric juice were separated into 8 peaks in the stomach cancer and into 6 peaks in the control. Amylase in the gastric juice was separated by the chromatography into 2 isozymes in the stomach cancer, but not in the control. Substrate specificity for the two gastric juice amylase isozymes was observed to be different. These results indicate that protein content and amylase activity in the gastric juice could be used as a biochemical marker for the stomach cancer and that amylases specific to the stomach cancer appear to be released from the stomach cancer tissue.

CYP2E1 Genetic Polymorphism with Dietary, Tobacco, Alcohol Habits, H. pylori Infection Status and Susceptibility to Stomach Cancer in Mizoram, India

Malakar, Mridul,Devi, K. Rekha,Phukan, Rup Kumar,Kaur, Tanvir,Deka, Manab,Puia, Lalhriat,Baruah, Debajit,Mahanta, Jagadish,Narain, Kanwar Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.20

Background: The incidence of stomach cancer in India is highest in the state of Mizoram. In this population based matched case-control study, we evaluated the relationship between CYP450 2E1 RsaI polymorphism and risk of stomach cancer taking into considering various important dietary habits along with tobacco, alcohol consumption and H. pylori infection status. Materials and Methods: A total of 105 histologically confirmed stomach cancer cases and 210 matched healthy population controls were recruited. CYP2E1 RsaI genotypes were determined by PCR-RFLP and H. pylori infection status by ELISA. Information on various dietary, tobacco and alcohol habits was recorded in a standard questionnaire. Results: Our study revealed no significant association between the CYP2E1 RsaI polymorphism and overall risk of stomach cancer in Mizoram. However, we observed a non-significant protective effect of the variant allele (A) of CYP2E1 against stomach cancer. Tobacco smokers carrying C/C genotype have three times more risk of stomach cancer, as compared to non-smokers carrying C/C genotype. Both Meiziol and cigarette current and past smokers who smoked for more than 10 times per day and carrying the (C/C) genotype are more prone to develop stomach cancer. Smoke dried fish and preserved meat (smoked/sun dried) consumers carrying C/C genotype possesses higher risk of stomach cancer. No significant association between H. pylori infection and CYP2E1 RsaI polymorphism in terms of stomach cancer was observed. Conclusions: Although no direct association between the CYP2E1 RsaI polymorphism and stomach cancer was observed, relations with different tobacco and dietary risk habits in terms of developing stomach cancer exist in this high risk population of north-eastern part of India. Further in-depth study recruiting larger population is required to shed more light on this important problem.

사매의 KATOIII 위암세포(胃癌細胞)에 대(對)한 항암효능(抗癌效能)

임중화,김진성,윤상협,류기원,류봉하,Lim, Joong-Hwa,Kim, Jin-Sung,Yoon, Sang-Hyub,Ryu, Ki-Won,Ryu, Bong-Ha 대한한방내과학회 2005 大韓韓方內科學會誌 Vol.26 No.2

Background : Death by cancer, malignant tumor, is on the increase around the world. In South Korea, cancer is the number one cause of death, and, in South Korea, stomach cancer is the most common form of cancer. This may be due to genetic and enviornmental factors. Because stomach cancer is so prevalent here, this study was undertaken. Objectives: This study was conducted to investigate the anti-cancer effects of Duchesnea indica(Andr.) Focke. in human stomach cancer cells(KATOIII). Materials & Methods: For human stomach cancer cells KATO III cells were obtained from Korea Cell Line Bank. The water decoction of Duchesnea indica(Andr.) Focke was added to cultures. First, morphological changes were examined under an inverted microscope. The destruction of stomach cancer cells was measured through Trypan blue exclusion testing, and suppression of viability of stomach cancer cells was measured via MTT assay. Anti-cancer mechanisms were assessed by analyzing the cell cycle. Results & Conclusions : In morphologic change, stomach cancer cells showed the withdrawn and floating appearance that is typical in cellular impairment. In each test group more stomach cancer cells were killed than in the control group to a statistically significant degree. Each test group showed more suppression of viability of stomach cancer cells than that of the control group to a statistically significant degree. Analysis of the cell cycle showed that Duchesnea indica(Andr.) Focke inhibited division of stomach cancer cells. This experiment yielded empirical evidence for Duchesnea indica(Andr.) Focke as a statistically significant anticarcinogen, specifically against stomach cancer.

춘천 지역에서의 위암 발생률에 관한 연구, 2000-2002년

김성중 ( Sung Jung Kim ),백광호 ( Gwang Ho Baik ),윤광희 ( Kwang Hee Youn ),송성욱 ( Sung Wook Song ),김동준 ( Dong Joon Kim ),김진봉 ( Jin Bong Kim ),민슬기 ( Seul Ki Min ),백일현 ( Il Hyun Baek ),박철희 ( Cheol Hee Park ),장현주 대한내과학회 2007 대한내과학회지 Vol.73 No.4

목적: 위암은 아직까지 국내 발생률 1위의 암으로 조기진단과 확진이 일선 의료기관에서 가능한 질환이다. 최근 Helicobacter pylori (H. pylori)가 위암의 원인으로 알려지면서 위암 역학에 대해서 관심이 고조되고 있다. 이번 연구는 춘천시 지역의 위암 발생률을 추정함으로써 조기위암을 발견하기 위한 노력과 함께 암관리사업과 향후 H. pylori 제균 후 위암 발생률의 변화를 알아 보기 위한 기초 자료를 만들고자 한다. 방법: 2000년 7월부터 만 2년간 춘천지역의 전 의료기관에서 내시경검사, 상부위장관 촬영의 자료를 수집하고 주민등록상 춘천시에 거주하는 환자를 대상으로 연령, 나이, 증상, 진단방법, 위내시경검사 소견, 치료 등 임상 특징을 조사하였다. 위암 진단은 병리 조직학적 확진이 있거나 내시경검사 소견과 상부위장관 촬영 소견이 모두 위암 소견을 보이는 경우로 하였다. 발생률 추정에 이용한 모집단은 2000-2002년 춘천시 인구의 중앙인구를 이용하였고, 연령과 성별에 따라 위암 환자의 분포와 조발생률을 구한 후 직접 표준화법을 이용하여 전국인구에 대한 연령 보정 표준화 발생률을 구하였다. 결과: 2년간 진단된 위암 환자는 총 186명으로 남자 117명, 여자 69명이었다. 조직학 진단을 통한 확진율은 97.8%이었다. 10만명 당 조발생률은 남자 47.0명, 여자 27.2명이었고, 전체적으로는 37.0명이었다. 2000-2002년의 우리나라의 중앙인구를 표준으로 한 연령 보정 표준화 발생률은 인구 10만명 당 33.7명이었고 남, 여 각각 인구 10만명 당 42.2, 25.1명이었다. 연령대별로는 남자의 경우 60대, 70대, 50대 순이었고, 여자의 경우 70대, 40대, 60대 순이었으며, 남녀 모두 합쳐서는 60대가 59명(31.7%)으로 가장 많은 연령대였다. 증상으로는 상복부통증이 제일 많았고, 그 외에 소화 불량, 체중 감소 등이었으며 무증상의 건강 검진 환자도 11.3%이었다. 위암의 내시경 분류로는 진행 위암이 112명(65.1%), 조기위암 60명(34.9%)이었다. 위암을 진단받은 환자 중 H. pylori검사를 한 환자는 86명으로 이 중 64명(74%)에서 양성이었다. 결론: 저자들은 2000-2002년에 춘천시 지역에서 발생한 위암의 발생률과 위암환자의 특성에 대하여 조사하였다. 이번 연구는 H. pylori가 감염된 환자에서 시행하는 제균치료가 향후 위암 환자의 발생률에 미치는 영향에 대한 기초 자료로 이용할 수 있다. Background: Stomach cancer is the most common cancer in Korea, and it can be easily diagnosed by performing endoscopy. Helicobacter pylori (H. pylori) is associated with stomach cancer, and there has been much recent interest in the epidemiology of stomach cancer. Therefore, we estimated the incidence of stomach cancer in Chuncheon-si during 2000-2002. Methods: From July 1st, 2000 through June 30th, 2002, we investigated the subjects who were confined to be newly diagnosed cases living in Chuncheon-si. The data was collected from the medical records from all the medical facilities located in Chuncheon-si. Results: The total number of cases of newly diagnosed stomach cancer during this period (2000-2002) was 186 (117 males and 69 females). The annual crude incidence rate of stomach cancer was estimated to be 37.0 per 100,000 persons overall, and 47.0 and 27.2 in the males and females, respectively. The annual age standardized incidence rate for the Korean population was 33.7 per 100,000 persons overall, and 42.2 and 25.1 for the males and females, respectively. The major symptoms of stomach cancer patients were epigastric pain (61.8%) and weight loss (31.2%). The proportion of early gastric cancer was 34.9% (60 among 172 persons) of the cases of stomach cancer. 64 patients (74%) were H. pylori positive. Conclusions: We report here on the estimated incidence rate of the stomach cancer and the characteristics of the stomach cancer patients in Chuncheon-si during 2000-2002. This study has generated basic epidemiologic data to identify the effect of H. pylori eradication on the future incidence rate of stomach cancer.(Korean J Med 73:368-374, 2007)

위암,대직장암 환자에서 혈청 CEA 및 CA 19-9의 수술전 및 수술 후의 변동에 관한 연구

하홍근,이승도 高神大學 醫學部 1989 高神大學醫學部 論文集 Vol.5 No.1

Genetic Polymorphism of Glutathione S-transferases M1 and T1, Tobacco Habits and Risk of Stomach Cancer in Mizoram, India

Malakar, Mridul,Devi, K. Rekha,Phukan, Rup Kumar,Kaur, Tanvir,Deka, Manab,Puia, Lalhriat,Barua, Debajit,Mahanta, Jagadish,Narain, Kanwar Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Aim: The incidence of stomach cancer in Mizoram is highest in India. We have conducted a population based matched case-control study to identify environmental and genetic risk factors in this geographical area. Methods: A total of 102 histologically confirmed stomach cancer cases and 204 matched healthy population controls were recruited. GSTM1 and GSTT1 genotypes were determined by PCR and H. pylori infections were determined by ELISA. Results: Tobacco-smoking was found to be an important risk factor for high incidence of stomach cancer in Mizoram. Meiziol (local cigarette) smoking was a more important risk factor than other tobacco related habits. Cigarette, tuibur (tobacco smoke infused water) and betel nut consumption synergistically increased the risk of stomach cancer. Polymorphisms of GSTM1 and GSTT1 genes were not found to be directly associated with stomach cancer in Mizoram. However, they appeared to be effect modifiers. Persons habituated with tobacco smoking and/or tuibur habit had increased risk of stomach cancer if they carried the GSTM1 null genotype and GSTT1 non-null genotype. Conclusion: Tobacco smoking, especially meiziol is the important risk factor for stomach cancer in Mizoram. GSTM1 and GSTT1 genes modify the effect of tobacco habits. This study is a first step in understanding the epidemiology of stomach cancer in Mizoram, India.

Spatial Analysis of Stomach Cancer Incidence in Iran

Pakzad, Reza,Khani, Yousef,Pakzad, Iraj,Momenimovahed, Zohre,Mohammadian-Hashejani, Abdollah,Salehiniya, Hamid,Towhidi, Farhad,Makhsosi, Behnam Reza Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.no.sup3

Stomach cancer, the fourth most common cancer and the second leading cause of cancer-related death through the world, is very common in parts of Iran. Geographic variation in the incidence of stomach cancer is due to many different factors. The aim of this study was to assess the geographical and spatial distribution of stomach cancer in Iran using data from the cancer registry program in Iran for the year 2009. The reported incidences of stomach cancer for different provinces were standardized to the world population structure. ArcGIS software was used to analyse the data. Hot spots and high risk areas were determined using spatial analysis (Getis-Ord Gi). Hot and cold spots were determined as more than or less than 2 standard deviations from the national average, respectively. A significance level of 0.10 was used for statistical judgment. In 2009, a total of 6,886 cases of stomach cancers were reported of which 4,891 were in men and 1,995 in women (standardized incidence rates of 19.2 and 10.0, respectively, per 100,000 population). The results showed that stomach cancer was concentrated mainly in northwest of the country in both men and women. In women, northwest provinces such as Ardebil, East Azerbaijan, West Azerbaijan, Gilan, and Qazvin were identified as hot spots (p<0.1). In men, all northwest provinces, Ardabil, East Azerbaijan, Gilan, Qazvin, Zanjan and Kurdistan, the incidences were higher than the national average and these were identified as hot spots (P<0.01). As stomach cancer is clustered in the northwest of the country, further epidemiological studies are needed to identify factors contributing to this concentration.