LncRNA PVT1 influences breast cancer cells glycolysis through sponging miR-145-5p

Qu Huan,Li Xingxing,Chen Fei,Zhang Min,Lu Xun,Gu Yun,Lv Mingming,Lu Cheng 한국유전학회 2023 Genes & Genomics Vol.45 No.5

Background Long-non-coding RNA PVT1 (lncRNA PVT1) can be used as an oncogenic regulatory non-coding RNA (ncRNA) for many cancers. However, its function and mechanism in breast cancer (BRCA) are still not clearly elucidated. Objective We attempt to explain the mechanism of PVT1’s role in breast cancer from different perspectives. Methods We analyzed the expression of PVT1 and its correlation with the breast cancer related clinical data in the The Cancer Genome Atlas (TCGA) database. We used PVT1 overexpression and knockdown lentivirus to infect breast cancer MDA-MB-231 cell line for cell function verification, in vitro using CCK-8 to measure proliferation, flow cytometry to measure apoptosis, transwell test to measure invasion and migration ability, detecting cell extracellular acidification rate (ECAR) to assess glycolysis metabolism and explore the biological functions of PVT1 in breast cancer cells. Transcriptome sequencing was used to analyze the changes of related genes in cells after overexpression of PVT1. In vivo we used a xenograft model to study the effect of PVT1 on breast cancer. Results PVT1 was up-regulated in breast cancer tissues and was positively correlated with the clinical stage of breast cancer patients. Overexpression of PVT1 in vitro promoted cell proliferation, migration and invasion, and promoted tumor growth in vivo. Knockdown of PVT1 led to the opposite biological consequence. Further bioinformatics analysis showed that PVT1 changes the glycolysis metabolism of tumors through regulation of glycolysis-related genes. In addition, the expression of miR-145-5p is negatively correlated with PVT1. We consider the possibility of PVT1 promoting cell proliferation and metastasis by regulating the aerobic glucose metabolism in breast cancer cells through sponging the miR-145-5p. Conclusion Our results reveal a potential pathway for competing endogenous RNA to regulate breast cancer glucose metabolism. PVT1 regulates glycolysis related genes expression by competitively binding to endogenous miR-145-5p in breast cancer cells to change the metabolic phenotype. This may Provide new ideas for precise molecular therapy targets for breast cancer.

실험에 의한 액체식 Unglazed PVT모듈의 성능 분석

천진아(Chun Jin-Aha),김진희(Kim Jin-Hee),강준구(Kang Jun-Gu),김준태(Kim Jun-Tae) 한국태양에너지학회 2010 한국태양에너지학회 학술대회논문집 Vol.2010 No.11

The heat from PV modules should be removed for better electrical performance, and can be converted into useful thermal energy. A photovoltaic-thermal(PVT) collector is a combination of PV module with a solar thermal collector which forms one device that converts solar radiation into electricity and heat simultaneously. In general, there are two different types of PVT collectors: glass-covered PVT module, which produces high-temperature heat but has a slightly lower electrical yield, and uncovered PVT module, which produces relatively low-temperature heat but has a somewhat higher electrical performance. This paper dealt with the electrical and thermal performance of water-type unglazed PVT collector, in relation to the inlet water temperature in particular. The experimental results showed that the thermal efficiency of the PVT module was 32.3% average and its PV efficiency was about 12.4% average, both depending on solar radiation, inlet water temperature and ambient temperature. The relationship between the PVT surface temperature and inlet water temperature into the unglazed PVT combined module was analysed. For the unglazed PVT combined module, it can be concluded that the PVT module required to maintain under a certain inlet water temperature to prevent the electrical performance decrease prevention depending on temperature increase.

감자T바이러스 검정을 위한 RT-PCR 및 Nested PCR 진단시스템 개발

이시원(Si Won Lee),신용길(Yong-Gil Shin),이진영(Jin-Young Lee),김영석(Young-Suk Kim),양미희(Mi Hee Yang),최인철(In-Cheol Choi) 충남대학교 농업과학연구소 2015 농업과학연구 Vol.42 No.2

Potato virus T (PVT) is a plant pathogen in the family Betaflexiviridae, group IV single-stranded positive sense RNA viruses. The major host of PVT is potato, and it has been reported in Ullucus tuberosus, Oxalis tuberosa and Tropaeolum tuberosum. This study aimed at developing reverse transcription (RT)-polymerase chain reaction (PCR) and nested PCR techniques for specific detection of PVT. Finally, Two RT-PCR primer sets were developed and verified. The RT-PCR products were amplified to 734 (PVT RT-PCR primer set 6) and 828 bp (PVT RT-PCR primer set 29) long to detect PVT. The nested PCR primer sets [PVT-N70/C20 (734→315 bp) and PVT-N75/C30 (828→529 bp)] were developed which are high sensitivity and verification for detection of PVT. Furthermore, a modified-positive control plasmid is use to verify contamination of laboratory in PVT detection. This study supported the diagnose PVT in potato or PVT related hosts.

감자T바이러스 검정을 위한 RT-PCR 및 Nested PCR 진단시스템 개발

이시원(Si Won Lee),신용길(Yong-Gil Shin),이진영(Jin-Young Lee),김영석(Young-Suk Kim),양미희(Mi Hee Yang),최인철(In-Cheol Choi) 충남대학교 농업과학연구소 2015 Korean Journal of Agricultural Science Vol.42 No.2

Potato virus T (PVT) is a plant pathogen in the family Betaflexiviridae, group IV single-stranded positive sense RNA viruses. The major host of PVT is potato, and it has been reported in Ullucus tuberosus, Oxalis tuberosa and Tropaeolum tuberosum. This study aimed at developing reverse transcription (RT)-polymerase chain reaction (PCR) and nested PCR techniques for specific detection of PVT. Finally, Two RT-PCR primer sets were developed and verified. The RT-PCR products were amplified to 734 (PVT RT-PCR primer set 6) and 828 bp (PVT RT-PCR primer set 29) long to detect PVT. The nested PCR primer sets [PVT-N70/C20 (734→315 bp) and PVT-N75/C30 (828→529 bp)] were developed which are high sensitivity and verification for detection of PVT. Furthermore, a modified-positive control plasmid is use to verify contamination of laboratory in PVT detection. This study supported the diagnose PVT in potato or PVT related hosts.

인공 신경망을 이용한 GTS 기반 PVT 집열기의 성능 예측 및 평가

이드아함드파지 ( Ahmed Fawzy Eid ),이상익 ( Sangik Lee ),이종혁 ( Jonghyuk Lee ),정영준 ( Youngjoon Jeong ),서병훈 ( Byunghun Seo ),김동수 ( Dongsu Kim ),서예진 ( Yejin Seo ),최원 ( Won Choi ) 한국농공학회 2022 한국농공학회 학술대회초록집 Vol.2022 No.-

Recently, Deep learning methods have been widely used in PVT collector systems based on artificial neural networks (ANNs) due to their high precision in evaluating the performances without using physical modeling. A photovoltaic thermal (PVT) collector is a hybrid system that merges a photovoltaic (PV) module and a thermal collector in one unit to generate electricity and heat simultaneously. In addition to that, it increases efficiency and reduces required costs and space. In this study, three different models of PVT collectors were fabricated based on Tedlar Polyester Tedlar (TPT), Graphite Thermal Sheet (GST), and TPT/GST as a back sheet and tested. An Ethylene Glycol Coolant (EGC) was also utilized with a water tank and a heat exchanger. The ANN model is used to predict PVT collectors’ electrical and thermal efficiencies based on measured weather datasets. Besides, both electrical and thermal models have been tested experimentally. Accurate modeling methods have been applied to generate the output results for training and validation. The actual climatic data and PVT collectors’ outputs of one year with a 5-minute step have been used to calculate the thermal and electrical efficiencies. The solar irradiance, wind speed, humidity, PVT collectors’ outputs, and temperature are the most critical variables to consider as input in the ANN-based model. This study’s expected results are increasing the PVT’s total efficiency by using high thermal conductivity material (GTS) instead of low thermal conductivity material (TPT). The electrical efficiency of the PVT module will increase due to the cooling effect. We also expect congruous results between the ANN analysis and experimental test.

PVT1 knockdown inhibited the biological behavior of LPS-induced cardiac fibroblasts by regulating miR-24

Dai Qing,Hong Yi,Li Jie 한국유전학회 2021 Genes & Genomics Vol.43 No.9

Background The heart is one of the target organs vulnerable to sepsis. About 50% of sepsis patients will sufer from myocardial injury and cardiac dysfunction, which will aggravate the sepsis and afect its prognosis. Objectives Here, we attempt to investigate the function of long non coding RNA PVT1 in LPS-induced cardiac fbroblasts in vitro, and explore its potential mechanism. Methods The expression of PVT1 in LPS-induced cardiac fbroblasts was detected by qRT-PCR. CCK-8 assay, cell migration, qRT-PCR and western blotting analysis were applied to evaluating the efect of PVT1 knockdown on LPS-induced cardiac fbroblasts. The bioinformatics analysis and the rescue experiment were devoted to the underlying mechanism. Results PVT1 expression was up-regulated in LPS-induced cardiac fbroblasts. And knockdown of PVT1 inhibited cell viability and migration, alleviated infammation cytokines production of LPS-treated cardiac fbroblasts. The bioinformatics analysis predicted PVT1 negatively regulates miR-24 and KLF6 is a direct target of miR-24. Conclusions In a word, we observed PVT1 expression level was up-regulated in LPS- treated cardiac fbroblasts. PVT1 knockdown could alleviate LPS-induced biological behavior of cardiac fbroblasts through sponging miR-24 in vitro.

PVT 변화 보상 기능을 가지는 시간-디지털 변환기

신은호,김종선 한국전기전자학회 2023 전기전자학회논문지 Vol.27 No.3

본 논문에서는 PVT(process, voltage, and temperature) 변화에 대한 보상기능을 가지는 시간-디지털 변환기(time-to-digitalconverter:TDC)를 제안한다. 일반적인 지연 라인(delay line) 기반의 TDC는 인버터의 전파 지연을 기반으로 시간을 측정하기 때문에 근본적으로 PVT 변화에 민감하다. 이 논문은 PVT 변화에 의한 전파 지연을 보상하여 TDC의 해상도 변화를 최소화시키는방법을 제안한다. 또한 넓은 입력 측정 범위(detection range)를 갖기 위해 Cyclic Vernier TDC (CVTDC) 구조를 채택한다. 제안하는 PVT보상 기능의 CVTDC는 45nm CMOS 공정으로 설계되어, 8mW의 전력을 소모하며, 5 ps의 TDC 해상도 및 약 5.1ns 입력 측정 범위를 갖는다. In this paper, we propose a time-to-digital converter (TDC) with compensation capability for PVT (process,voltage, and temperature) variations. A typical delay line-based TDC measures time based on the inverter’s propagationdelay, making it fundamentally sensitive to PVT variations. This paper presents a method to minimize theresolution change of TDC by compensating for the propagation delay caused by the PVT variations. Additionally,it dopts Cyclic Vernier TDC (CVTDC) structure to provide a wide input detection range. The proposed CVTDC withPVT compensation function is designed using a 45nm CMOS process, consumes 8mW of power, offers a TDCresolution of 5 ps, and has an input detection range of about 5.1 ns.

계절별 PV 및 PVT의 효율 비교를 통한 공기식 PVT 시스템의 태양에너지 활용 효과 분석

김강현,최영진 한국태양에너지학회 2022 한국태양에너지학회 논문집 Vol.42 No.4

Recently, the use of new and renewable energy for suppressing the generation of carbon dioxide to prevent global warming has attracted considerable attention. Among the various new and renewable energy sources, solar energy has been attracting increasing interest for the reduction of building energy, as it is easy to implement in buildings and excellent for maintenance and repair. Therefore, in this study, an air-based photovoltaic thermal (PVT) system, which can increase the utilization of solar energy, was compared with the existing PV system through measurements. The PVT system can increases the amount of power generated by reducing the temperature of the panel via the air passing through the lower part of the panel. Furthermore, useing the air whose temperature has been increased by the heat obtained from the panel for indoor heating or hot water supply in the building is possible. The performance of existing PV and PVT panels was measured under the same weather conditions, and result indicated that the power generation efficiency of PVT panels, through which the air passes was higher than that of PV panels. Furthermore, the air whose temperature was increased to ≥40°C by the PVT system may be utilized for heating and hot water supply. Overall, by utilizing solar power, air-based PVT systems can utilize 3.4 ~ 3.9 times more solar power than can conventional PVs.

Knockdown of lncRNA PVT1 Inhibits Vascular Smooth Muscle Cell Apoptosis and Extracellular Matrix Disruption in a Murine Abdominal Aortic Aneurysm Model

Zhidong Zhang,Gangqiang Zou,Xiaosan Chen,Wei Lu,Jianyang Liu,Shuiting Zhai,Gang Qiao 한국분자세포생물학회 2019 Molecules and cells Vol.42 No.3

This study was designed to determine the effects of the long non-coding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) on vascular smooth muscle cell (VSMC) apoptosis and extracellular matrix (ECM) disruption in a murine abdominal aortic aneurysm (AAA) model. After injection of PVT1-silencing lentiviruses, AAA was induced in Apolipoprotein E-deficient (ApoE?/?) male mice by angiotensin II (Ang II) infusion for four weeks. After Ang II infusion, mouse serum levels of pro-inflammatory cytokines were analysed, and aortic tissues were isolated for histological, RNA, and protein analysis. Our results also showed that PVT1 expression was significantly upregulated in abdominal aortic tissues from AAA patients compared with that in controls. Additionally, Ang II treatment significantly increased PVT1 expression, both in cultured mouse VSMCs and in AAA murine abdominal aortic tissues. Of note, the effects of Ang II in facilitating cell apoptosis, increasing matrix metalloproteinase (MMP)-2 and MMP-9, reducing tissue inhibitor of MMP (TIMP)-1, and promoting switching from the contractile to synthetic phenotype in cultured VSMCs were enhanced by overexpression of PVT1 but attenuated by knockdown of PVT1. Furthermore, knockdown of PVT1 reversed Ang II-induced AAA-associated alterations in mice, as evidenced by attenuation of aortic diameter dilation, marked adventitial thickening, loss of elastin in the aorta, enhanced aortic cell apoptosis, elevated MMP-2 and MMP-9, reduced TIMP-1, and increased proinflammatory cytokines. In conclusion, our findings demonstrate that knockdown of lncRNA PVT1 suppresses VSMC apoptosis, ECM disruption, and serum pro-inflammatory cytokines in a murine Ang II-induced AAA model.

Knockdown of lncRNA PVT1 Inhibits Vascular Smooth Muscle Cell Apoptosis and Extracellular Matrix Disruption in a Murine Abdominal Aortic Aneurysm Model

Zhang, Zhidong,Zou, Gangqiang,Chen, Xiaosan,Lu, Wei,Liu, Jianyang,Zhai, Shuiting,Qiao, Gang Korean Society for Molecular and Cellular Biology 2019 Molecules and cells Vol.42 No.3

This study was designed to determine the effects of the long non-coding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) on vascular smooth muscle cell (VSMC) apoptosis and extracellular matrix (ECM) disruption in a murine abdominal aortic aneurysm (AAA) model. After injection of PVT1-silencing lentiviruses, AAA was induced in Apolipoprotein E-deficient ($ApoE^{-/-}$) male mice by angiotensin II (Ang II) infusion for four weeks. After Ang II infusion, mouse serum levels of pro-inflammatory cytokines were analysed, and aortic tissues were isolated for histological, RNA, and protein analysis. Our results also showed that PVT1 expression was significantly upregulated in abdominal aortic tissues from AAA patients compared with that in controls. Additionally, Ang II treatment significantly increased PVT1 expression, both in cultured mouse VSMCs and in AAA murine abdominal aortic tissues. Of note, the effects of Ang II in facilitating cell apoptosis, increasing matrix metalloproteinase (MMP)-2 and MMP-9, reducing tissue inhibitor of MMP (TIMP)-1, and promoting switching from the contractile to synthetic phenotype in cultured VSMCs were enhanced by overexpression of PVT1 but attenuated by knockdown of PVT1. Furthermore, knockdown of PVT1 reversed Ang II-induced AAA-associated alterations in mice, as evidenced by attenuation of aortic diameter dilation, marked adventitial thickening, loss of elastin in the aorta, enhanced aortic cell apoptosis, elevated MMP-2 and MMP-9, reduced TIMP-1, and increased pro-inflammatory cytokines. In conclusion, our findings demonstrate that knockdown of lncRNA PVT1 suppresses VSMC apoptosis, ECM disruption, and serum pro-inflammatory cytokines in a murine Ang II-induced AAA model.