태음인(太陰人) 청심연자탕(淸心蓮子湯)이 Hydrogen Peroxide에 손상(損傷)된 백서(白鼠)의 대뇌신경세포(大腦神經細胞)에 미치는 영향(影響)

옥윤영,유도곤,김경요,Ok, Yun-young,Ryu, Do-gon,Kim, Kyung-yo 사상체질의학회 1999 사상체질의학회지 Vol.11 No.2

1. 연구목적 본 실험은 청심연자탕이 대뇌신경세포의 산화적 손상에 대한 효능을 밝히기 위한 것이다. 2. 연구방법 신생 생쥐에서 분리 배양한 대뇌신경세포에 여러 농도의 hydrogen peroxide가 포함된 배양액에서 6시간 동안 처리하여 hydrogen peroxide가 배양 대뇌신경세포에 미치는 영향을 조사하였으며 hydrogen peroxide의 독성효과에 대한 청심연자당의 영향을 조사하였다. 3. 결과 및 결론 산소자유기인 hydrogen peroxide는 NR assay와 MTT assay에 의한 세포생존율을 감소시켰고 lipid peroxidation의 증가 및 LDH양의 증가에 의하여 생쥐의 배양 대뇌신경세포에 독성을 나타냈다. 청심연자탕(淸心蓮子湯)은 hydrogen peroxide의 산화적 손상에 대한 신경독성에 대하여 Iipid peroxidation의 감소에 유의한 효과를 보였다. 청심연자탕(淸心蓮子湯)은 hydrogen peroxide의 산화적 손상에 의한 신경독성에 대하여 LDH양의 감소에 유의한 효과를 보였다. 이상의 결과로 보아 hydrogen peroxide는 생쥐에서 분리한 대뇌신경세포에 산화적 손상에 의한 신경독성을 나타냈으며 청심연자탕(淸心蓮子湯)이 hydrogen peroxide와 같은 산소자유기의 산화적 손상에 대한 방어에 효과적인 것으로 사료된다. 1. Purpose : The purpose of this study was to determine the effects of Chungsimyeunjatang on the cerebral neurons injured by hydrogen peroxide($H_2O_2$). 2. Methods : I observed cell viability in mouse cerebral neurons exposed to hydrogen peroxide by NR assay and MTT assay and determined lipid peroxidation and amounts of LDH release in mouse cerebral neurons exposed to hydrogen peroxide. After administration of Chungsimyeunjatang water extracts, I observed significant changes of cell viability, lipid peroxidation and amounts of LDH release in mouse cerebral neurons exposed to hydrogen peroxide. 3. Results : Hydrogen peroxide showed neurotoxicity. Cell viability in mouse cerebral neurons exposed to hydrogen peroxide decreased in NR assay and MTT assay. Lipid peroxidation and amounts of LDH release in mouse cerebral neurons exposed to hydrogen peroxide increased. Chungsimyeunjatang was very effective in blocking hydrogen peroxide-induced neurotoxicity.

Glutamine Deprivation Causes Hydrogen Peroxide-induced Interleukin-8 Expression via Jak1/Stat3 Activation in Gastric Epithelial AGS Cells

Lee, Yun Mi,Kim, Mi Jung,Kim, Youngha,Kim, Hyeyoung Korean Society of Cancer Prevention 2015 Journal of cancer prevention Vol.20 No.3

<P><B>Background:</B></P><P>The Janus kinase (Jak)/Signal transducers of activated transcription (Stat) pathway is an upstream signaling pathway for NF-κB activation in <I>Helicobacter pylori</I>-induced interleukin (IL)-8 production in gastric epithelial AGS cells. <I>H. pylori</I> activates NADPH oxidase and produces hydrogen peroxide, which activates Jak1/Stat3 in AGS cells. Therefore, hydrogen peroxide may be critical for IL-8 production via Jak/Stat activation in gastric epithelial cells. Glutamine is depleted during severe injury and stress and contributes to the formation of glutathione (GSH), which is involved in conversion of hydrogen peroxide into water as a cofactor for GSH peroxidase.</P><P><B>Methods:</B></P><P>We investigated whether glutamine deprivation induces hydrogen peroxide-mediated IL-8 production and whether hydrogen peroxide activates Jak1/Stat3 to induce IL-8 in AGS cells. Cells were cultured in the presence or absence of glutamine or hydrogen peroxide, with or without GSH or a the Jak/Stat specific inhibitor AG490.</P><P><B>Results:</B></P><P>Glutamine deprivation decreased GSH levels, but increased levels of hydrogen peroxide and IL-8, an effect that was inhibited by treatment with GSH. Hydrogen peroxide induced the activation of Jak1/Stat3 time-dependently. AG490 suppressed hydrogen peroxide- induced activation of Jak1/Stat3 and IL-8 expression in AGS cells, but did not affect levels of reactive oxygen species in AGS cells.</P><P><B>Conclusions:</B></P><P>In gastric epithelial AGS cells, glutamine deprivation increases hydrogen peroxide levels and IL-8 expression, which may be mediated by Jak1/Stat3 activation. Glutamine supplementation may be beneficial for preventing gastric inflammation by suppressing hydrogen peroxide-mediated Jak1/Stat3 activation and therefore, reducing IL-8 production. Scavenging hydrogen peroxide or targeting Jak1/Stat3 may also prevent oxidant-mediated gastric inflammation.</P>

2.6% Hydrogen Peroxide 부착대(strip)의 치아미백효과에 관한 실험실(In vitro) 실험연구

정세환,박덕영,마득상,김지영,김종호 대한구강보건학회 2002 大韓口腔保健學會誌 Vol.26 No.3

A laboratory trial was conducted to evaluate post-treatment color improvement following bleaching with 2.6% hydrogen peroxide containing tooth-whitening strip. Hydroxyapatite tablet(HPT) was made by compressing and sintering 2.5 g hydroxyapatite powder. HPTs were embedded in epoxy resin except active surface. This active surface was stained using Stookey' s methods. Bleaching materials were 2.6% hydrogen peroxide strip, 5,3% hydrogen peroxide strip(Crest^ Whitestips), 2.6% hydrogen peroxide solution, and placebo strip without hydrogen peroxide. HPTs were allocated to each bleaching materials by matching on color grade. After HPTs were treated for 30 minutes at 37℃ and washed and dried, color was measured by Colorimeter CR-321 (Minolta, Japan). The efficacy was evaluated by comparing delta L and delta E_ab values of HPTs surface at baseline and each repeated steps. There was no change of L' value in placebo strip. Each delta L* and delta E'ab values of 2.6% hydrogen peroxide strip, 5.3% hydrogen peroxide strip(Crest Whitestips), and 2.6% hydrogen peroxide solution was increased significantly compared to baseline and placebo strip, respectively (P<0.05). There was no significant change in delta L and delta E_ab values among 2.6% hydrogen peroxide strip, 5.3% hydrogen peroxide strip(Crest^ Whitestips), and 2.6% hydrogen peroxide solution. Whitening effect of 2.6% hydrogen peroxide strip was fairly high compared to 5.3% hydrogen peroxide strip in this in vitro experiment, but further in vivo experiment is needed to prove its efficacy.

A Study on the Damage to the Bleached Hair Based on the Concentration of Hydrogen Peroxide

( Woo-been Kim ),( Ji-hyeon Im ),( Je-hee Park ),( Eun-jun Park ) 한국미용예술경영학회 2012 미용예술경영연구 Vol.6 No.4

This paper relates to the investigation into the color difference of hair, physical changes in hair, and morphological changes of hair, which aimed to determine the effect of hair damage caused by bleaching agent based on the concentration of hydrogen peroxide. The results of the investigation were as follows In relation to the color difference of hair, L*a*b values increased as concentration of hydrogen peroxide increased to 3%, 6%, and 9%. The L*value and b*value for 6% and 9% increased even more as the concentration of hydrogen peroxide increased. The hair thickness decreased as the concentration of hydrogen peroxide increased to 3%, 6%, and 9%. Also, the tensile strength of hair decreased as the concentration of hydrogen peroxide increased. There was no significant difference in the morphological changes at 3% concentration of hydrogen peroxide, compared to health hair. However, the cuticle scale layers, which had been piled one upon another, decreased and the hair became loose as the concentration of hydrogen peroxide increased to 6% and 9%. In addition, the results of experiment showed that the cuticle of bleached hair was damaged the most in the 9% concentration of hydrogen peroxide. Based on the hair decolorization of hydrogen peroxide concentration was increased above results, the increase of hair color changes, so you can easily appear due to the decrease of the tensile strength and the thickness of the hair, but the hair cuticle damage, because the concentration of hydrogen peroxide 6%▯ is considered to be suitable.

Tooth whitening effect according ot the concentration of hydrogen peroxide

Lee, Jae-Min Shin, Seung-Chul Cho, Ja-Won Choi, Yun-Hwa Moon, Yang-Mi Jung, Su-Jin Kwon, Jung-Hee 대한임상예방치과학회 2007 International Journal of Clinical Preventive Denti Vol.3 No.1

Objectives: The authors investigated tooth whitening effect according to the concentration of hydrogen peroxide. Methods: 60 healthy anterior teeth which had extracted in a recent month were selected, and classified into 3 groups as 15% hydrogen peroxide tooth whitening group, 22% group, and 35% group. Tooth whitening had performed 3 times for 20 minutes/day for 3 days with applying each concentration hydrogen peroxide solution. Vita shade manual guide and Shade scan were used for checking the value, hue and the translucency by manual or computer program. The data were analysed and compared the before and after tooth whitening. Results: The changes of the tooth colors were examined as the increase of B strain colors and the decease of C strain colors on higher hydrogen peroxide concentration tooth whitening group. There was no statistically difference between the value changes of 3 groups(p>0.05), measured by Vita shade manual guide. There was no statistically difference between the value changes of 3 groups(p>0.05), measured by Shadescan. Level 5 translucency, as high value of the translucency on the tooth surface was increased in 15% hydrogen peroxide tooth whitening group(p>0.05), decreased in 22% group(p>0.05), but there were no significant difference between the two groups statistically. And Level 5 translucency statistically decreased in 35% group(p<0.05). Level 1 translucency, as low value of the translucency on the tooth surface was decreased in 15% hydrogen peroxide tooth whitening group(p>0.05), increased in 22% group(p>0.05), and increased in 35% group(p>0.05). Conclusion: It was recommended to use prefer the 15% or 22% hydrogen peroxide than 35% with some activating method, for effective tooth whitening to promote the hue of the tooth color without changing the translucency the irritation on tooth surface, in clinical preventive dentistry.

Monitoring and Characterization of Bacterial Contamination in a High-Purity Water System Used for Semiconductor Manufacturing

Kim, In -Seop,Lee, Geon-Hyoung,Lee, Kye-Joon The Microbiological Society of Korea 2000 The journal of microbiology Vol.38 No.2

Hydrogen peroxide has been used in cleaning the piping of an advanced high-purity water system that supplies ultra-high purity water (UHPW) for 16 megabyte DRAM semiconductor manufacturing. The level of hydrogen peroxide-resistant bacteria in UHPW water was monitored prior to and after disinfecting the piping with hydrogen peroxide. Most of the bacteria isolated after hydrogen peroxide disinfection were highly resistant to hydrogen peroxide. However, the percentage of resistant bacteria decreased with time. The hydrogen peroxide-resistant bacteria were identified as Micrococcus luteus, Bacillus cereus, Alcaligenes latus, Xanthomonas sp. and Flavobacterium indologenes. The susceptibility of the bacteria to hydrogen peroxide was tested as either planktonic cells or attached cells on glass. Attached bacteria as the biofilm on glass exhibited increased hydrogen peroxide resistnace, with the resistance increasing with respect to the age of the biofilm regrowth on piping after hydrogen peroxide treatment. In order to optimize the cleaning strategy for piping of the high-purity water system, the disinfecting effect of hydrogen preoxide and peracetic acid on the bacteria was evaluated. The combined use of hydrogen peroxide and peracetic acid was very effective in killing attached bacteria as well as planktonic bacteria.

Detection of Hydrogen Peroxide in vitro and in vivo Using Peroxalate Chemiluminescent Micelles

Iljae Lee,강길선,Donghyuck Yoo,On Hwang,이동원 대한화학회 2011 Bulletin of the Korean Chemical Society Vol.32 No.7

Hydrogen peroxide plays a key role as a second messenger in the normal cellular signaling but its overproduction has been implicated in various life-threatening diseases. Peroxalate chemiluminescence is the light emission from a three component reaction between peroxalate, hydrogen peroxide and fluorophores. It has proven great potential as a methodology to detect hydrogen peroxide in physiological environments because of its excellent sensitivity and specificity to hydrogen peroxide. We developed chemiluminescent micelles composed of amphiphilic polymers, peroxalate and fluorescent dyes to detect hydrogen peroxide at physiological concentrations. In this work, we studied the relationship between the chemiluminescence reactivity and stability of peroxalate by varying the substitutes on the aryl rings of peroxalate. Alkyl substitutes on the aryl ring of peroxalate increased the stability against water hydrolysis, but diminished the reactivity to hydrogen peroxide. Chemiluminescent micelles encapsulating diphenyl peroxalate showed significantly higher chemiluminescence intensity than the counterpart encapsulating dimethylphenyl or dipropylphenyl peroxalate. Diphenyl peroxalate-encapsulated micelles could detect hydrogen peroxide generated from macrophage cells stimulated by lipopolysaccharide (LPS) and image hydrogen peroxide generated during LPS-induced inflammatory responses in a mouse.

Detection of Hydrogen Peroxide in vitro and in vivo Using Peroxalate Chemiluminescent Micelles

Lee, Il-Jae,Hwang, On,Yoo, Dong-Hyuck,Khang, Gil-Son,Lee, Dong-Won Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.7

Hydrogen peroxide plays a key role as a second messenger in the normal cellular signaling but its overproduction has been implicated in various life-threatening diseases. Peroxalate chemiluminescence is the light emission from a three component reaction between peroxalate, hydrogen peroxide and fluorophores. It has proven great potential as a methodology to detect hydrogen peroxide in physiological environments because of its excellent sensitivity and specificity to hydrogen peroxide. We developed chemiluminescent micelles composed of amphiphilic polymers, peroxalate and fluorescent dyes to detect hydrogen peroxide at physiological concentrations. In this work, we studied the relationship between the chemiluminescence reactivity and stability of peroxalate by varying the substitutes on the aryl rings of peroxalate. Alkyl substitutes on the aryl ring of peroxalate increased the stability against water hydrolysis, but diminished the reactivity to hydrogen peroxide. Chemiluminescent micelles encapsulating diphenyl peroxalate showed significantly higher chemiluminescence intensity than the counterpart encapsulating dimethylphenyl or dipropylphenyl peroxalate. Diphenyl peroxalate-encapsulated micelles could detect hydrogen peroxide generated from macrophage cells stimulated by lipopolysaccharide (LPS) and image hydrogen peroxide generated during LPS-induced inflammatory responses in a mouse.

Characterization of Inducible Oxidative Stress Response in Vitreoscilla C₁

Youn, Myung-Ja,Choi, Yun-Young,Park, Kie-In Korean Society of Molecular Biology 2001 Molecules and cells Vol.11 No.2

Development of a thermally self-sustaining kW_e-class diesel reformer using hydrogen peroxide for hydrogen production in low-oxygen environments

Han, Gwangwoo,Lee, Kwangho,Ha, Sanghyeon,Bae, Joongmyeon Elsevier 2016 Journal of Power Sources Vol.326 No.-

<P><B>Abstract</B></P> <P>A novel technology of a diesel reformer that uses hydrogen peroxide is developed to obtain the hydrogen required for fuel cell air-independent propulsion for underwater applications, such as submarines and unmanned underwater vehicles. Diesel fuel could be a promising hydrogen source for underwater applications due to its high hydrogen density and its globally well-equipped infrastructure. An alternative oxidant, hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>), is applied to supply not only oxygen but also the water required for diesel autothermal (ATR) reforming. The proposed reformer does not require an additional heating device to supply heat for the vaporization of diesel or oxidant due to the exothermic nature of the ATR reaction and the heat of decomposition of H<SUB>2</SUB>O<SUB>2</SUB>. The effects of H<SUB>2</SUB>O<SUB>2</SUB> on diesel reforming were confirmed based on operating the engineering-scale (kW<SUB>e</SUB>-class) diesel-H<SUB>2</SUB>O<SUB>2</SUB> reformer. Undecomposed H<SUB>2</SUB>O<SUB>2</SUB> caused an excessively high temperature in the mixing zone and a corrosion effect in the reformer wall. To overcome these phenomena, we introduced a catalytic H<SUB>2</SUB>O<SUB>2</SUB> decomposer to fully decompose hydrogen peroxide into steam and oxygen. From this important step, we essentially eliminate side effects from undecomposed H<SUB>2</SUB>O<SUB>2</SUB> and retain a high reforming efficiency by utilizing the heat of decomposition of H<SUB>2</SUB>O<SUB>2</SUB>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Novel technology of a kW<SUB>e</SUB>-class diesel reformer with hydrogen peroxide was developed. </LI> <LI> Thermally self-sustaining operation is possible without any heating devices. </LI> <LI> Reforming characteristics were analyzed when operated with hydrogen peroxide. </LI> <LI> Hydrogen peroxide decomposer was newly introduced to resolve side effects. </LI> <LI> Reformer design points were suggested when hydrogen peroxide is used as an oxidant. </LI> </UL> </P>